Abstract:
:Activated macrophages are known to release a variety of immunoregulatory substances including the low-molecular-weight substances hydrogen peroxide and lactate. We report here that lactate but not hydrogen peroxide is capable of supporting a substantial production of T-cell growth factor (TCGF) in cultures of accessory cell-depleted splenic T-cell populations after stimulation with concanavalin A. Hydrogen peroxide and its biosynthetic precursor superoxide anion (O2-) mediate, however, a strong augmentation of the TCGF production by accessory cell-depleted T-cell populations in the presence of lactate. Lactate inhibits the incorporation of [3H]thymidine in short-term cultures (18-26 hr) of accessory cell-depleted T cells. This confirms the rule that (optimal) production of T-cell growth factor requires a growth inhibitory signal. Concentrations of hydrogen peroxide which augment TCGF production most effectively (i.e., 1 X 10(-5) M) do not inhibit the incorporation of [3H]thymidine; and higher concentrations (3 X 10(-5)-1 X 10(-4) M) of hydrogen peroxide inhibit both the production of TCGF and the incorporation of [3H]thymidine. In agreement with the augmenting effect of hydrogen peroxide on TCGF production, it was observed that the proliferative response in mixed lymphocyte cultures is suppressed by catalase and augmented by 1 X 10(-5) M H2O2. Proliferative and cytotoxic responses in mixed lymphocyte cultures with an external source of interleukin 2 (IL-2) in contrast, are not augmented by 1 X 10(-5) M H2O2. The relatively high concentration of 1 X 10(-4) M hydrogen peroxide was found to inhibit the proliferative responses in mixed lymphocyte cultures with or without external IL-2 but not the cytotoxic response in the presence of IL-2. This indicates that CTL precursor cells may be relatively resistant against H2O2.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Roth S,Dröge Wdoi
10.1016/0008-8749(87)90224-3subject
Has Abstractpub_date
1987-09-01 00:00:00pages
417-24issue
2eissn
0008-8749issn
1090-2163pii
0008-8749(87)90224-3journal_volume
108pub_type
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90166-1
更新日期:1983-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Cellular immunology
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更新日期:1986-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1050
更新日期:1995-04-15 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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更新日期:2018-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1997-09-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90207-4
更新日期:1989-05-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1985-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2001.1883
更新日期:2001-11-25 00:00:00