Abstract:
:Five thiol compounds including 2-mercaptoethanol (2-ME) were examined for their augmenting effects on in vitro antibody response to sheep erythrocytes. Three compounds were effective with the following order of activity; 2-ME greater than dithiothreitol greater than cysteamine. Glutathione and thioglycollate failed to enhance the response. The same order or effectiveness was seen in the stimulation of [35S]cystine uptake by murine lymphocytes by these thiols. Murine lymphocytes took up cysteine five to six times more rapidly than cystine. It is, however, unlikely that 2-ME stimulation of cystine uptake is solely due to the reduction of cystine into cysteine, because 2-ME was still stimulatory after free thiol groups had disappeared in the medium containing 2-ME and [35S]cystine. The mixed disulfide of cysteine with 2-ME (Cys-2-ME) was found to be an only product after free thiols had been oxidized. [35S]Cys-2-ME was taken up by the lymphocytes with a comparable rate to cysteine via a transport system common to that of leucine and phenylalanine. Cysteine was, however, transported via a different route. It was observed that Cys-2-ME was readily metabolized to cysteine and glutathione after the uptake. Cys-2-ME added to cystine-free RPMI 1640 medium could support the antibody response as efficiently as cystine plus 2-ME. These observations strongly suggest that 2-Me stimulates cystine uptake and, therefore, enhances the antibody response through the formation of the mixed disulfide with cysteine.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Ohmori H,Yamamoto Idoi
10.1016/0008-8749(83)90060-6subject
Has Abstractpub_date
1983-07-01 00:00:00pages
173-85issue
1eissn
0008-8749issn
1090-2163pii
0008-8749(83)90060-6journal_volume
79pub_type
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1038
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journal_title:Cellular immunology
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更新日期:1986-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90372-6
更新日期:1983-03-01 00:00:00
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更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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更新日期:2009-01-01 00:00:00
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doi:10.1016/j.cellimm.2011.03.009
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abstract:AIMS:Evaluate the participation of IL-17 pathway in T1D pathogenesis. T helper 17 cells are potent, highly inflammatory cells that produce interleukin 17A (IL-17A), considered a mediator of various immune disorders. However, their role in Type 1 diabetes (T1D) pathogenesis in humans is not totally elucidated. METHODS:...
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更新日期:2018-02-01 00:00:00