Abstract：
:Tumor-associated chemokines, including CC chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2), are thought to play many roles in cancer progression. Here we demonstrate the novel finding that during growth of the D1-7,12-dimethylbenzanthracene-3 mammary tumor in BALB/c mice, there is a dramatic up-regulation of CCL2 in splenic T cells at both the mRNA and protein levels upon stimulation. Of particular relevance is the finding that tumor-infiltrating T cells also produce high levels of CCL2. While a variety of tumor cell lines have been found to produce CCL2, we found no detectable levels of CCL2 protein in supernatants of the cultured mammary tumor cells. Investigation of the mechanisms involved in CCL2 induction showed that treatment of splenic T cells with the tumor-derived factors GM-CSF and phosphatidyl serine (PS) resulted in increased CCL2 production. This increased production may be involved in the downregulation of IFN-gamma by the T cells of tumor-bearing mice previously reported in this model, as treatment of splenic T lymphocytes with CCL2 resulted in a decreased secretion of IFN-gamma by those cells.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Owen JL,Lopez DM,Grosso JF,Guthrie KM,Herbert LM,Torroella-Kouri M,Iragavarapu-Charyulu Vdoi
10.1016/j.cellimm.2005.08.032subject
Has Abstractpub_date
2005-06-01 00:00:00pages
122-35issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(05)00192-9journal_volume
235pub_type
杂志文章abstract：:Antigen-nonspecific CD8+ T suppressor cells, which suppressed delayed-type hypersensitivity (DTH) against sheep red blood cells in BALB/c mice, were induced by incubating spleen cells from mice treated with 7,12-dimethylbenz[a]anthracene (DMBA), a tumor initiator, with 12-O-tetradecanoylphorbol 13-acetate (TPA), a tum...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(91)90167-a
更新日期：1991-12-01 00:00:00
abstract：:Inflammatory leukocytes of DA-to-WF rat renal allografts displayed significant cytolytic activity to natural killer (NK) target cells on Day 2 after transplantation. The NK activity, which was associated with large granular lymphocytes in discontinuous Percoll gradients, peaked on Day 4 and disappeared rapidly thereaf...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90342-3
更新日期：1984-12-01 00:00:00
abstract：:This study addresses the nature of the pathogenic effector T cell in experimental autoimmune uveoretinitis and the effect of different cytokines on these cells in vitro. Lymph node cells of B10.RIII mice immunized with the uveitogenic peptide 161-180 of interphotoreceptor retinoid binding protein were cultured with th...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1997.1121
更新日期：1997-05-25 00:00:00
abstract：:Murine histocompatibility antigens were solubilized from the spleens and lungs of C57BL/6 (H-2b) animals with hypertonic salt (3 M KC1). Aggregate-free soluble antigens were incubated with nonadherent lymph node cells from BALB/c (H-2d) mice for 18 hr prior to their use as responder cells in the mixed-lymphocyte react...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90281-2
更新日期：1983-06-01 00:00:00
abstract：:Putative monocytic myeloid-derived suppressor cells (mMDSC; lineage-HLA-DR-/lo) were generated in 7-day cultures from normal rhesus macaque bone marrow (BM) cells in GM-CSF and IL-6. Three subsets were identified based on their differential expression of CD14, CD33, CD34 and CD11b. Following flow sorting, assessment o...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.04.013
更新日期：2018-07-01 00:00:00
abstract：:Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is caused by mutations in the AIRE gene. Murine studies suggest that AIRE controls thymic expression of tissue-restricted antigens, its absence allowing nonselected autoreactive cells to escape. We tested this in humans using the TCRβ CDR3 length ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2015.03.005
更新日期：2015-06-01 00:00:00
abstract：:The biological effects of IL-2 are mediated through high (complex of alpha and beta chain) or intermediate (beta chain) affinity IL-2 receptors. Previously, chimeric proteins composed of IL-2 and Pseudomonas exotoxin (IL-2-PE) were shown to be specifically cytotoxic to cells bearing IL-2 receptors. It has also been sh...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90029-o
更新日期：1992-09-01 00:00:00
abstract：:Cells from the draining lymph nodes of DBA/2 mice bearing syngeneic intradermal P-815 tumors represent an excellent responding cell population for secondary stimulation in vitro, despite the virtual absence of response by spleen cells of the same animals. Cytotoxicity is a result of stimulation with intact mitomycin-t...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90255-1
更新日期：1983-05-01 00:00:00
abstract：:Experimental autoimmune thyroiditis is induced in mice by immunization with thyroglobulin emulsified in Freund's complete adjuvant. The disease is characterized both by thyroid infiltration with mononuclear cells and by circulating thyroglobulin antibodies. The magnitude of the thyroid infiltration and the titer of th...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90302-9
更新日期：1987-10-01 00:00:00
abstract：:The role of macrophages (M phi) in the in vitro primary antibody response of murine lymphocytes to sheep erythrocytes was investigated. Peritoneal M phi were activated to express Ia antigens either in vitro or in vivo. Nonactivated Ia- M phi were also examined. We observed that only Ia- M phi but also Ia+ M phi failed...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90316-8
更新日期：1985-10-15 00:00:00
abstract：:The effects of LTs are mediated by GPCRs: cysLTs interact with CYSLTR1, CYSLTR2, or GPR17, and LTB4 acts via BLT1R or BLT2R. Data relating to the presence of these receptors in mature tissue mast cells are not entirely known. By confocal microscopy with image analyses and flow cytometry, we established that native rat...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2017.04.010
更新日期：2017-07-01 00:00:00
abstract：:Recent studies have indicated that Macrophage migration inhibitory factor (MIF) plays an important role in the prevention and treatment of asthma. However the role of MIF in airway inflammation and airway epithelial barrier disruption in house dust mite (HDM)-induced asthma has not been addressed. We hypothesized that...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2019.103965
更新日期：2020-01-01 00:00:00
abstract：:PD-1 is an immunoregulatory receptor expressed on the surface of activated T cells, B cells, and monocytes. We describe four alternatively spliced PD-1 mRNA transcripts (PD-1Deltaex2, PD-1Deltaex3, PD-1Deltaex2,3, and PD-1Deltaex2,3,4) in addition to the full length isoform. PD-1Deltaex2 and PD-1Deltaex3 are generated...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2005.07.007
更新日期：2005-06-01 00:00:00
abstract：:The activation of large granular lymphocytes (LGLs)/natural killer (NK) cells with interleukin-2 (IL-2) has been shown to increase the ability of these cells to lyse NK-resistant tumor target cells. Activated LGLs, termed LAK (lymphokine-activated killer) cells, have been demonstrated to be of therapeutic value in viv...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90132-b
更新日期：1990-05-01 00:00:00
abstract：:It is becoming increasingly clear that regulatory T cells (Treg cells) in specific tissues are important parts of immune system. Tissue-resident Treg cells, which are largely Foxp3-expressing CD4(+) Treg cells, are distinct from one another and conventional Treg cells, and have tissue-specific phenotype and function. ...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2015.06.005
更新日期：2015-09-01 00:00:00
abstract：:The effect of prior maternal immunization on the murine offspring response to subsequent immunization with hen egg-white lysozyme was examined. Adult female A/J mice were immunized with 100 micrograms HEL-CFA intraperitoneally 10-27 weeks before conception. The offspring of these experimental female mice were then imm...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1123
更新日期：1993-05-01 00:00:00
abstract：:We investigated the time course of the 17 beta-estradiol effect on mouse natural killer (NK) activity and the number of splenic large granular lymphocytes (LGL), a cell population recently associated with natural cytotoxicity and enriched in low density fractions of Percoll discontinuous density gradients. After 7 day...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90163-8
更新日期：1987-05-01 00:00:00
abstract：:Vibrio vulnificus, a pathogenic bacterium causing primary septicemia, exhibited cytotoxicity towards Jurkat cells of T-lymphocytes through intracellular reactive oxygen species (ROS) production. Pretreatment of Jurkat T-cells with diphenyleneiodonium chloride (DPI) abolished V. vulnificus-induced ROS generation and ba...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.05.003
更新日期：2008-05-01 00:00:00
abstract：:Anti-CD3 mAb can activate T cells to help in B cell activation as detected by late events, such as maturation of B cells into Ig-secreting cells (IgSC), or by early events, such as B cell surface expression of the activation marker CD23. Two different anti-CD2 mAb each inhibited anti-CD3-induced T cell-dependent B cel...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90269-w
更新日期：1990-10-15 00:00:00
abstract：:Experimental autoimmune neuritis (EAN) is a T-cell-mediated autoimmune disease characterized by demyelination and mononuclear cell infiltration of the peripheral nervous system. It is induced in Lewis rats by administration of myelin P2 protein or a synthetic peptide (SP-26) corresponding to amino acid residues 53-78 ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1156
更新日期：1994-06-01 00:00:00
abstract：:Varying the concentration of anti-CD3 immobilized on Sepharose beads allowed us to study both the inhibitory and the stimulatory effects of IL-4 on purified T cells and contrast IL-4 versus IL-2-driven T-cell proliferative responses. In the presence of low density immobilized anti-CD3, IL-4 was unable to stimulate pur...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1081
更新日期：1993-04-01 00:00:00
abstract：:This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34(+)CD38(-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.07.008
更新日期：2006-05-01 00:00:00
abstract：:Double immunofluorescence studies for terminal deoxynucleotidyl transferase (TdT) and leucocyte surface membrane antigens have been used to characterize the small subpopulation of TdT-positive cells in human peripheral blood. The predominant antigens demonstrated were those coded for by the major histocompatibility co...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90223-0
更新日期：1985-02-01 00:00:00
abstract：:Recombinant IL-4 inhibits IL-2-induced lymphokine-activated killer (LAK) cell development of PBMC. We evaluated the effect of various cytokines in reversing IL-4-mediated LAK inhibition. PBMC were cultured in IL-2 (10-1000 u/ml) with or without IL-4 (2-100 u/ml) and tested for cytotoxicity against the NK-sensitive K56...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90040-x
更新日期：1990-07-01 00:00:00
abstract：:We tested the effect of different inhibitors of cytochrome P450 on tumor necrosis factor (TNF) production. Metyrapone and SKF525A (100 and 50 mg/kg, ip, respectively) suppressed serum TNF induced by cotreatment with endotoxin (LPS), (2.5 micrograms/mouse). Inhibition was independent of endogenous corticosteroids since...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1209
更新日期：1993-09-01 00:00:00
abstract：:Monoclonal-nonspecific suppressor factor (MNSF) is a lymphokine derived from murine T cell hybridoma. The target tissues are both LPS-stimulated B cells and Con A-stimulated T cells. Since the action of MNSF may be mediated by its binding to specific cell surface receptors, we characterized the mode of this binding. T...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(90)90271-r
更新日期：1990-10-15 00:00:00
abstract：:Neuroendocrine hormones such as growth hormone (GH) and prolactin (PRL) have been demonstrated to accelerate the recovery of the immune response after chemotherapy and bone marrow transplantation and to enhance the restoration of immunity in individuals infected with HIV and in normal individuals with compromised immu...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2007.12.003
更新日期：2008-03-01 00:00:00
abstract：:Medicinal plants are considered immunomodulatory as they display various biological activities. There is no report addressing the anti-inflammatory effects of Daphne gnidium. In this study, we investigated the effects of D. gnidium ethyl acetate (EA) leaf extract on mice immune cell function in vitro. Production of pr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.12.002
更新日期：2011-01-01 00:00:00
abstract：:It has been previously shown that the activated form of Factor B (Factor Bb) of the alternative pathway of complement activation stimulates monocyte spreading and killing of xenogenic erythrocytes and staphylococci. Factor Bb also stimulates lymphocyte blastogenesis in vitro, and native (uncleaved) Factor B is a major...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90005-x
更新日期：1985-05-01 00:00:00
abstract：:Human leucocytes from peripheral blood and tonsil were examined for the presence of the IL-3 receptor using monoclonal antibodies directed to epitopes of the alpha and beta chains of the receptor. We found that the beta chain, common to IL-3, IL-5, and GM-CSF, was either present at low levels or not detected on the ma...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0049
更新日期：1996-02-25 00:00:00
