Abstract:
:Varying the concentration of anti-CD3 immobilized on Sepharose beads allowed us to study both the inhibitory and the stimulatory effects of IL-4 on purified T cells and contrast IL-4 versus IL-2-driven T-cell proliferative responses. In the presence of low density immobilized anti-CD3, IL-4 was unable to stimulate purified T cells and was inhibitory to IL-2-driven T-cell responses. The inhibitory effects of IL-4 were enhanced by preincubation of T cells with IL-4 prior to stimulation. In contrast, the inhibitory effects of IL-4 could be avoided by delaying the addition of IL-4 until Days 3-5 of culture or they could be reversed by the addition of IL-1. In the presence of high-density anti-CD3, IL-4 elicited an IL-2-independent proliferative response by purified T cells or sorted CD8+ cells. Comparison of IL-4-driven versus IL-2-driven T-cell responses demonstrated that IL-2 was able to upregulate mRNA for IL-4 receptors and interferon-gamma, while IL-4 had minimal effects on upregulating mRNA for either the p55 or the p75 IL-2 receptor subunits or interferon-gamma. The timing of the presence of IL-4, the state of T-cell activation, and the nature and strength of the stimulatory signal influenced the regulatory effect of IL-4 on the immune response.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Jones-Tiffany LA,Mehrotra PT,Horohov DW,Siegel J,Kozak RWdoi
10.1006/cimm.1993.1081subject
Has Abstractpub_date
1993-04-01 00:00:00pages
425-37issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(83)71081-6journal_volume
147pub_type
杂志文章abstract::Endogenous retroviral superantigens such as vSAG-7 are highly stimulatory for T cells through interaction with the T cell receptor on the basis of V beta usage. Priming of adult MMTV 7-negative mice with vSAG-7-expressing cells has been shown to result in peripheral V beta 6/CD4+ T cell activation followed by toleranc...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1995.1164
更新日期:1995-09-01 00:00:00
abstract::FOXP3+ regulatory T cells (Tregs) constitute a critical barrier that enforces tolerance to both the self-peptidome and the extended-self peptidome to ensure tissue-specific resistance to autoimmune, allergic, and other inflammatory disorders. Here, we review intuitive models regarding how T cell antigen receptor (TCR)...
journal_title:Cellular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.cellimm.2020.104173
更新日期:2020-09-01 00:00:00
abstract::Using an in vitro model, we demonstrate that when CD4 T cells from HIV infected subjects are enriched from total blood lymphocytes the immune response to antigen is augmented. However, augmentation of this response is confined to HIV infected subjects with relatively preserved CD4 T cell counts. Enriching for CD4 T ce...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2006.09.003
更新日期:2006-07-01 00:00:00
abstract::Purine nucleoside phosphorylase (PNP) is a purine salvage pathway enzyme which we have found to be 8-10 times more active (per cell) in human peripheral blood null lymphocytes than in T lymphocytes. To test the hypothesis that null cells are, in part, pre-T lymphocytes we have defined an in vitro system for null cell ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90288-5
更新日期:1983-06-01 00:00:00
abstract::A low-virulence, agerminative strain of Candida albicans (PCA-2) is able to confer a high degree of nonspecific protection against subsequent challenge with highly virulent microorganisms in mice. In an attempt to better define the effect of PCA-2 vaccination on the immune system and the nature of the mechanisms invol...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90135-4
更新日期:1989-12-01 00:00:00
abstract::Although most intraepithelial lymphocytes (IEL) in mouse small intestine bear surface markers classically associated with T lymphocytes, the T-cell nature of these cells remains controversial. In the present study IEL from normal mice, or from mice infected with the gut nematode Trichinella spiralis, were therefore te...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90093-6
更新日期:1986-12-01 00:00:00
abstract:BACKGROUND:Interleukin (IL)-23, composed of p19 and p40 subunits, has diverse functions in regulating immune systems, enhancing cell-mediated immunity. In the present study, we investigated whether forced expression of the p19-linked p40 gene in murine mammary cancer cells (MA891) produced antitumor effects in vivo. Tu...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.05.002
更新日期:2009-01-01 00:00:00
abstract::Recently, it has been reported that IL-4 production by murine Th2 cell lines is insensitive to inhibition by E-type prostaglandins. In the present study, IL-4 production in vitro by freshly isolated concanavalin A (Con A)-stimulated murine spleen cells was readily suppressed by PGE2 with an I50 of 2 nM. Comparable sup...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(95)80039-l
更新日期:1995-02-01 00:00:00
abstract::Conalbumin (CA)-specific type 2 helper T cell (Th2) clone, D10G4.1 (D10) produces IL4 when stimulated with varying doses of TNP-CA in the presence of mitomycin C-treated C3H spleen cells or purified B cells as antigen-presenting cells (APC). The production of IL4 was assessed by bioassay and by expression of IL4 mRNA....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(92)90333-k
更新日期:1992-12-01 00:00:00
abstract::Putative monocytic myeloid-derived suppressor cells (mMDSC; lineage-HLA-DR-/lo) were generated in 7-day cultures from normal rhesus macaque bone marrow (BM) cells in GM-CSF and IL-6. Three subsets were identified based on their differential expression of CD14, CD33, CD34 and CD11b. Following flow sorting, assessment o...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.04.013
更新日期:2018-07-01 00:00:00
abstract::We have recently established a new animal model for primary Sjögren's syndrome in NFS/sld mutant mice thymectomized 3 days after birth (3dTX) bearing an autosomal recessive gene with sublingual gland differentiation arrest. In this study, we analyze developing mechanisms of experimental autoimmune sialadenitis (EAS) i...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0196
更新日期:1996-08-01 00:00:00
abstract::Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (alpha 1 and alpha 2) of H-2Kb and the carboxy-terminal domains (alpha 3, transmembrane, and ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90235-2
更新日期:1986-04-15 00:00:00
abstract::We have studied the antibody-induced effect of cross-linking the major histocompatibility complex class I (MHC-I) in human T leukemic cells (Jurkat) and human B lymphoma cells (Solubo, Burkitts lymphoma) on intracellular [Ca2+]i levels. The increase in [Ca2+]i after MHC-I cross-linking in Jurkat cells and Solubo cells...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0281
更新日期:1996-11-01 00:00:00
abstract::Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes w...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(83)90313-1
更新日期:1983-01-01 00:00:00
abstract::The purpose of this study was to determine whether multiple types of suppressor factors play a role in the regulation of immune responses by ultraviolet radiation-induced suppressor T lymphocytes (UV Ts). The UV Ts were induced by applying contact allergens to the ventral, unirradiated skin of mice exposed 5 days earl...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90007-5
更新日期:1989-06-01 00:00:00
abstract::Intercellular adhesion molecule (ICAM)-1, or CD54, is a member of the immunoglobulin superfamily that binds to lymphocyte function-associated antigen-1 and macrophage-1 antigen. ICAM-1:LFA-1/Mac-1 interaction may be involved in both activation and extravasation of leukocytes. To determine the roles of ICAM-1 in the de...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1998.1395
更新日期:1998-11-25 00:00:00
abstract::Non-obese diabetic (NOD) mice develop spontaneous T-cell responses against pancreatic beta-cells, leading to islet cell destruction and diabetes. Despite high genetic similarity, non-obese resistant (NOR) mice do not develop diabetes. We show here that spleen cells of both NOD and NOR mice respond to the islet cell an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2005.05.003
更新日期:2005-05-01 00:00:00
abstract::Mature thymocytes are usually defined and separated from other less mature thymocytes on the basis of their mutually exclusive expression of either CD4 or CD8. However, such murine "single positives" include a subpopulation of immature cells with properties resembling CD4- CD8- thymocytes or CD4+ CD8+ cortical blasts....
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90042-1
更新日期:1988-05-01 00:00:00
abstract::The effect of the addition of dialyzable leukocyte extract (DLE)(transfer factor) to tuberculostatic drugs in the treatment of superinfected fistulating tuberculosis of bones and joints was evaluated in a controlled study. Eleven patients whose disease had persisted for a mean of 20 +/- 4.8 years and had proved to be ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90091-1
更新日期:1984-03-01 00:00:00
abstract::We now report two healthy individuals whose T lymphocytes were over 95% positive for CD45RA antigen expression. However, these donors normally expressed both the CD29 high (CD29+) and CD45RO high (CD45RO+) antigens on approximately 40 and 50% of their CD4 cells, respectively. Despite the strong CD45RA expression on th...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(91)90089-t
更新日期:1991-10-15 00:00:00
abstract::We analyzed the effect of adherent cells on the induction of lymphokine-activated killer (LAK) cell activity by depleting adherent cells from peripheral blood lymphocytes (PBL) or by adding adherent cells to PBL before culture with interleukin-2. We found that adherent cells clearly down-regulate LAK cell induction. T...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90129-8
更新日期:1987-12-01 00:00:00
abstract::Controversies remain about NK cells direct responsiveness to Toll-like receptor (TLR) agonists or dependence on macrophages. In a melanoma lung metastasis model, aerosolized TLR9 and TLR3 agonists have been reported to induce antitumor immunity through NK cells activation. In the current study, we demonstrated that in...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2017.01.004
更新日期:2017-03-01 00:00:00
abstract::Possible causes were examined for the inability of heat-inactivated lymphocytes to induce proliferative responses in mixed-lymphocyte cultures (MLC). Cells heated at 45 degrees C for 60 min lost greater than 90% of their capacity to stimulate in primary (1 degree) or secondary (2 degrees) MLC. This was not due to acce...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90156-5
更新日期:1986-09-01 00:00:00
abstract::The candidacidal activity (CA) of peritoneal cells (PC) in vitro was used as a measure of nonspecific microbicidal activity of phagocytes after intraperitoneal injection of mice with different adjuvants. Dilutions of PC were incubated with constant numbers of C. parapsilosis in a 96-well culture plate. The PC number c...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90164-9
更新日期:1985-01-01 00:00:00
abstract::Patients treated with monoclonal antibodies and cytokines for cancer receive often co-medication, which may influence treatment efficacy. Therefore, we investigated with a flowcytometric cytotoxicity assay the effect of several immunomodulatory drugs on antibody dependent cellular cytotoxicity (ADCC), interleukin-2 (I...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.05.002
更新日期:2007-03-01 00:00:00
abstract::Antigen-specific T-helper factor (ThF) of CBA (H-2k) origin in the picryl (TNP) contact sensitivity system (Mr 60-70 kDa) was reduced with dithiothreitol under mild conditions. Affinity chromatography on antigen yielded an antigen-binding chain (Mr 20-30 kDa) and an antigen-nonbinding chain (Mr 40-50 kDa). Both chains...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90037-8
更新日期:1988-05-01 00:00:00
abstract::Abnormalities in immune response play a major role in the increased susceptibility to infection after hemorrhage and trauma. Infections occurring after injury often originate in the intestine. In order to determine the effects of hemorrhage on intestinal B cell function, we examined hemorrhage-induced alterations in a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90015-j
更新日期:1990-06-01 00:00:00
abstract::Polyunsaturated fatty acid [omega-3 polyunsaturated fatty acids (omega-3PUFAs)] incorporation into cell membranes has been shown to have potent anti-inflammatory activity, though the mechanisms involved are only partially characterized. Here, we show that PUFA enrichment of T cell membranes decreased the overall expre...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2004.04.004
更新日期:2004-04-01 00:00:00
abstract::Semaphorins are important regulators of peripheral T and B-cell mediated immune responses in mice and humans. Modulatory roles of semaphorins in T cell development are also being characterized. We carefully analyzed the gene expression and protein levels of semaphorins 4A, 4D, and 7A at various developmental stages of...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2013.03.006
更新日期:2013-02-01 00:00:00
abstract::The aim of this study was to analyze in vivo the L3T4+ T-cell-subset-independent reactivity of Lyt2+ T cells toward transplantation alloantigens. To this end, we depleted normal mice of L3T4+ T cells by injection of monoclonal antibodies to the L3T4 antigen. This procedure not only led phenotypically to a disappearanc...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90059-7
更新日期:1988-01-01 00:00:00