Abstract:
:The effects of IL-10 on in vitro antigen-driven murine antibody responses and helper cell IL-4 and IFN-gamma secretory capacity were investigated. Low antigen concentrations stimulated high responses in all antibody isotypes examined; IgD was not assayed. Under these conditions, exogenous IL-10 minimally potentiated synthesis of antigen-specific IgM, IgG1, IgG2a, IgG2b, IgG3, and IgA, but inhibited antigen-specific IgE secretion. High antigen levels stimulated antigen-specific IgM, IgG2a, and IgG2b responses, but inhibited synthesis of all other isotypes. In high antigen cultures, IL-10 augmented secretion of antigen-specific antibody in all isotypes except IgE. Essentially all of the antibody produced in the presence of high or low antigen concentrations was antigen-specific. Exogenous IL-10 substantially stimulated production of antigen-nonspecific antibody in all isotypes except IgG3. IL-10 allowed for greater Ig+ cell yield; comparable numbers of CD4+ and CD8+ cells were observed in the presence or absence of IL-10 in culture. The stimulatory effects of IL-10 for in vitro antibody responses were observed during a limited period of time after in vivo antigen priming of the responding cell populations. In contrast, IL-10 inhibited IgE synthesis at all time points tested. Low concentrations of antigen maintained the in vitro capacity of helper cells to secrete IL-4, while high antigen concentrations did not. Exogenous IL-10 potentiated IL-4 secretory capacity in high antigen cultures. The capacity for IFN-gamma secretion was comparable in high and low antigen cultures and exogenous IL-10 significantly inhibited such capacity under both sets of conditions. We conclude that IL-10 is generally stimulatory for murine antibody responses in vitro, with the possible exception of antigen-specific IgE. Such stimulatory effects appear to reflect increased activity of type 2 helper cells with concurrent decrease in type 1 helper cell activity.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Marcelletti JF,Katz DHdoi
10.1006/cimm.1996.0011subject
Has Abstractpub_date
1996-01-10 00:00:00pages
86-98issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(96)90011-8journal_volume
167pub_type
杂志文章abstract::Activin A is a multifunctional factor of the transforming growth factor-beta (TGF-beta) superfamily and acts as an anti-inflammatory cytokine produced by microglia and macrophages. In this study, we investigated the regulatory effect and possible mechanism of activin A on activation of lipopolysaccharide (LPS)-induced...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.11.001
更新日期:2009-01-01 00:00:00
abstract::The induction of ornithine decarboxylase (ODC) activity in lymphocytes is associated with activation and the initiation of cellular proliferation. ODC is also an essential component in tumor promotion. Phorbol myristic acetate (PMA) is a mitogen for lymphocytes, but can also promote tumor formation. Tumor promotion is...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90015-9
更新日期:1988-04-15 00:00:00
abstract::Patients with head and neck cancer often have decreased local or regional immunocompetence. Lymphocytes obtained from tumor-involved or -uninvolved lymph nodes (LNL) of these patients showed low or undetectable levels of antitumor cytotoxicity and low proliferative responses in vitro to interleukin 2 (IL2) or mitogens...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90286-z
更新日期:1990-10-15 00:00:00
abstract::Immunohistochemistry and flow cytometry analyses were performed to characterize the specific T cell subpopulations infiltrating basal cell carcinomas (BCC) and to determine their phenotypic response to in vitro expansion with IL-2. Tumor-infiltrating lymphocytes (TIL) within BCC predominantly expressed the CD45RO (act...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1038
更新日期:1993-02-01 00:00:00
abstract::Neutrophils exhibit intrinsic sinusoidal metabolite concentration oscillations of 3 min in resting cells and an additional approximately 10- or 20-s oscillation in migrating/adhering cells. To better understand immune complex (IC)-mediated leukocyte activation, we have studied neutrophil metabolic oscillations in the ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1481
更新日期:1999-05-25 00:00:00
abstract::The proliferation of Molt-4 lymphoblasts and phytohemagglutinin-stimulated human T lymphocytes in vitro was inhibited significantly by 10(-12) to 10(-10) M to 10(-13) to 10(-9) M somatostatin, as assessed by the uptake of [3H]thymidine and [3H]leucine, respectively. The inhibitory effect of somatostatin was not attrib...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90117-5
更新日期:1984-04-01 00:00:00
abstract::Toll-like receptors are a family of pattern-recognition receptors that contribute to the innate immune response. Toll-like receptor 3 (TLR3) signals in response to foreign, endogenous and synthetic ligands including viral dsRNA, bacterial RNA, mitochondrial RNA, endogenous necrotic cell mRNA and the synthetic dsRNA an...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.10.002
更新日期:2007-08-01 00:00:00
abstract::PICOT (PKC-interacting cousin of thioredoxin) consists of one thioredoxin homology domain in the N-terminal and two tandem PICOT homology domains in the C-terminal. PICOT specifically interacts with protein kinase C theta (PKC-theta) via its thioredoxin homology domain and acts as an important modulator of T cell rece...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2008.04.005
更新日期:2008-01-01 00:00:00
abstract::We have reported that Burkitt lymphomas (BL) that arise in HLA-A11 positive individuals are resistant to lysis by HLA-A11-specific and HLA-A11-restricted CTLs(10,11). Here we show that this phenomenon can be explained by a selective loss of the HLA-A11 polypeptide. The HLA-A11 negative phenotype is due to a regulatory...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90207-4
更新日期:1989-05-01 00:00:00
abstract::NK and extrathymic T cells are abundant in the decidua of the pregnant uterus. To determine how this unique pattern is induced, overall populations of leukocytes were examined in the blood and other tissues in pregnant women. Time-kinetic studies showed that a basal change of leukocytes during pregnancy was granulocyt...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1999.1541
更新日期:1999-08-25 00:00:00
abstract::T lymphocytes respond to mitogenic stimulation by expressing the receptor for interleukin 2 (Il-2) and secreting Il-2; once the receptor is expressed, Il-2 induces these cells to proliferation. In the present report using mouse T lymphocytes, thymocytes, and the lymphoma cell line EL4, we studied receptor expression a...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90096-1
更新日期:1986-12-01 00:00:00
abstract::We investigated the effects of prostaglandin E2 and IL-4 on the acquisition of cytokine-producing ability by naive CD4(+) T cells in human umbilical cord blood. The presence of PGE2 or IL-4 at primary stimulation inhibited the production of IFN-gamma at secondary stimulation, and the combination of these stimuli resul...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1180
更新日期:1997-10-10 00:00:00
abstract::The primary and secondary responses to Tnp-Ficoll, a class 2 thymus-independent antigen, were assessed in various inbred strains of mice. The eventual implication of H-2 or IgH linked genes was searched for. Contrasting with our previous reports using Tnp-LPS, a class 1 thymus-independent antigen, no homologous memory...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90135-7
更新日期:1984-08-01 00:00:00
abstract::We previously reported that T cell proliferation in response to a primary signal through the T cell receptor (TCR) and a costimulatory signal via the CD28 molecule is impaired in healthy, aged mice. Here we extend these studies to examine factors which may be involved in this defect in T cells from aged mice. To deter...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0144
更新日期:1996-05-25 00:00:00
abstract::The relay hypothesis [R. Nayak, S. Mitra-Kaushik, M.S. Shaila, Perpetuation of immunological memory: a relay hypothesis, Immunology 102 (2001) 387-395] was earlier proposed to explain perpetuation of immunological memory without requiring long lived memory cells or persisting antigen. This hypothesis envisaged cycles ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.05.001
更新日期:2007-03-01 00:00:00
abstract::In view of the eminently important role of interleukin-2 (IL-2) in T-cell responses, and in view of reports about immune stimulatory effects of PGE2, we reinvestigated the question whether PGE2 inhibits IL-2 production. It was found that PGE2 does not inhibit IL-2 production in murine spleen cell cultures after optima...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90180-7
更新日期:1985-01-01 00:00:00
abstract::We have studied the effects of prostaglandin E2 (PGE2) and cholera toxin, two modulators of adenylyl cyclase, and 8-bromo cAMP (8-BrcAMP) on various parameters of lymphocyte activation using the human T cell line Jurkat. Our results show that PGE2 and cholera toxin inhibit, in a dose-related manner, the phytohemagglut...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90035-p
更新日期:1990-07-01 00:00:00
abstract::Enteric immunization with schistosome ova results in a diminished granulomatous response. This study explored a mechanism by which enteric immunization may decrease granuloma size. Granulomas from livers of acutely infected mice were dissociated and the dispersed cells were depleted of macrophages. As defined by a dir...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(87)90227-9
更新日期:1987-09-01 00:00:00
abstract::We investigated the influence of Panagen DNA preparations on laboratory animals and IFN-induced human dendritic cells, as well as analyzed the data from a phase II clinical trial in the therapy of breast cancer. It was shown that this treatment resulted in increased number of CD8+/perforin+ T cells in peripheral lymph...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2012.04.004
更新日期:2012-03-01 00:00:00
abstract::Twenty-seven different CD45 monoclonal antibodies (mAb) were assessed for their ability to block cytotoxicity of alloreactive CD4+ MHC class II-specific or CD8+ class I-specific human T cell clones (n = 3 and 5, respectively). Twelve of 27 blocked the former but only 1/27 the latter, although all 27 significantly inhi...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90151-g
更新日期:1990-05-01 00:00:00
abstract::T-Cell hybridomas were constructed by fusing BW5147, an AKR lymphoma, with concanavalin A-stimulated murine splenic lymphocytes. The hybrids which were formed were studied for their ability to produce a lymphokine which inhibits tumor cell migration (TMIF) as well as macrophage migration (MIF) using in vitro assays. C...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90158-3
更新日期:1985-07-01 00:00:00
abstract::The third complementarity determining region (CDR3) is the most variable part of alpha beta T cell receptors (TCR) and represents the putative antigen contacting site. To identify parameters determining the structural diversity of the CDR3 region, the CDR3 length distributions of 66 BV-J combinations in peripheral CD4...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1996.0071
更新日期:1996-03-15 00:00:00
abstract::Pentraxin 3 (PTX3) is a prototype protein of long pentraxin. PTX3 is produced by various cells, such as monocytes/macrophages (Mphis) in response to lipopolysaccharide (LPS) and proinflammatory signals. We performed immunoblotting, immunohistochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR),...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2007.09.003
更新日期:2007-08-01 00:00:00
abstract::Phagocytosis of pathogens by macrophages is crucial for the successful resolution of inflammation induced by microbial infection. Taurine chloramine (TauCl), an endogenous anti-inflammatory and antioxidative substance, is produced by reaction between taurine and hypochlorous acid by myeloperoxidase activity in neutrop...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.02.003
更新日期:2018-05-01 00:00:00
abstract::The cellular requirements for the in vitro induction of antigen-specific suppressor T cells were examined. Previous reports indicated that Ia-bearing macrophages and anti-idiotypic B cells are required as accessory cells to facilitate the generation of suppressor effector (TS3) cells which regulate the response to the...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90242-0
更新日期:1988-10-15 00:00:00
abstract::The antifungal activity of nonactivated resident murine peritoneal macrophages for Cryptococcus neoformans was studied. Macrophages from five of six mouse strains tested had significant (40 to 80%) fungistatic activity, depending on the inoculum size, in a 24-hr coculture system. Macrophages from two outbred (SW and I...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1200
更新日期:1994-08-01 00:00:00
abstract::Adoptive immunotherapy is a promising cancer treatment that entails infusion of immune cells manipulated to have antitumor specificity, in vitro. Antigen-specific cytotoxic T lymphocytes are the main executors of transformed cells during cancer immunotherapy. To induce antigen-specific cytotoxic T lymphocytes, we deve...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2018.10.002
更新日期:2018-12-01 00:00:00
abstract::Humans with autoimmune disease and autoimmune mouse strains such as NZB have been shown to produce reduced levels of the cytokines interleukin 1 and interleukin 2. The NZB X C58 recombinant inbred (N X 8 RI) strains exhibit certain of the autoimmune characteristics of the NZB strain. Their abilities to produce interle...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90177-1
更新日期:1984-10-15 00:00:00
abstract::Hybrid human-murine major histocompatibility antigens have been constructed and expressed on the surface of both human RD and murine L cell lines after DNA mediated gene transfer. These antigens linked the polymorphic domains (alpha 1 and alpha 2) of H-2Kb and the carboxy-terminal domains (alpha 3, transmembrane, and ...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90235-2
更新日期:1986-04-15 00:00:00
abstract::Enriched human B lymphocytes cocultivated with mouse L cells produced human leukocyte interferon (IFN-alpha) and shortly thereafter transferred antiviral activity to the recipient cells (99% inhibition of expected virus yield). In contrast, cocultivation of enriched T-cell populations with mouse L cells resulted in no...
journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90035-2
更新日期:1984-09-01 00:00:00