Morphology of sweat glands in determining time of death.


:This study demonstrates post-mortem autolytic alterations in the skin at cellular and subcellular levels and identifies parameters which may assist in determining the time of death in the first few hours post-mortem. Serial skin samples from the ventral surface of the arm were taken at intervals of 3, 6, 9 and 12 h after death in 29 subjects of various ages, with no signs of skin disease; causes of death were various. Three types of tests were performed: cytochemical (hematoxylin-eosin and alcian-PAS), immunohistochemical (S-100, CEA, Cytokeratin, ASM) and ultrastructural (electron microscopy). Electron microscopy proved useful for identifying transformations which were found to be specific for each chronological step considered: reduction of intracellular glycogen in clear cells and reduction of secretory granules in dark cells are typical signs of the first stage (3 h) after death; mitochondrial dilatation and rarefaction of cristae in clear and dark cells are typical of the second stage (6 h); rarefaction of microvilli in dark and clear cells is a sign of the last stage (12 h). Cytochemistry and immunohistochemistry supply useful information--not for all the chronological stage considered here, but for individual phases (3 h for hematoxylin-eosin and 6 h for alcian-PAS). However, it is particularly important to use the results from all such techniques simultaneously, so that the question of the exact time of death within the first 12 h post-mortem may be more accurately answered.


Int J Legal Med


Cingolani M,Osculati A,Tombolini A,Tagliabracci A,Ghimenton C,Ferrara SD




Has Abstract


1994-01-01 00:00:00












  • Sudden cardiac death in hereditary hemochromatosis: an underestimated cause of death?

    abstract::Hereditary hemochromatosis (HH) is a frequent autosomal recessive disease which causes iron-overload of various organs. Of all northern European affected individuals, 90-95% show 1 of 3 known point mutations in the HFE gene. Symptoms and organs involved can vary considerably: Only a small fraction of the 200,000-400,0...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Klintschar M,Stiller D

    更新日期:2004-06-01 00:00:00

  • Perspiration versus saliva--basic aspects concerning their use in roadside drug testing.

    abstract::Various aspects concerning the practical application and forensic interpretation of data obtained by saliva drug testing and drug monitoring from the skin surface are discussed. Basic information on the composition of saliva and skin secretions and their particular transport mechanisms, as far as known, are given. For...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章,评审


    authors: Skopp G,Pötsch L

    更新日期:1999-01-01 00:00:00

  • Sequence of melanocyte migration into human scar tissue.

    abstract::Scars of human skin can on occasions provide a very useful ancillary method of identification of an unknown deceased. If the age of any visible scars can be estimated objectively, then this might be of some assistance in the identification procedure. Melanocytes migrate into scar tissue as it ages and their number wit...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Dressler J,Busuttil A,Koch R,Harrison DJ

    更新日期:2001-10-01 00:00:00

  • Identification and characterization of two novel human polymorphic STRs on the Y chromosome.

    abstract::From sequence database information, we have identified two male-specific and polymorphic tetranucleotide STRs, DYS 441 (GDB:10013873) and DYS 442 (GDB: 10030304), on the Y chromosome. Analysis of 184 males allowed 7 and 5 alleles to be distinguished in the DYS 441 and DYS 442 systems, respectively, yielding 21 haploty...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Iida R,Tsubota E,Matsuki T

    更新日期:2001-08-01 00:00:00

  • Forensic identification of urine samples: a comparison between nuclear and mitochondrial DNA markers.

    abstract::Urine samples from 20 male volunteers of European Caucasian origin were stored at 4 degrees C over a 4-month period in order to compare the identification potential of nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) markers. The amount of nDNA recovered from urines dramatically declined over time. Consequently, nDNA ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Castella V,Dimo-Simonin N,Brandt-Casadevall C,Robinson N,Saugy M,Taroni F,Mangin P

    更新日期:2006-03-01 00:00:00

  • Crack propagation through sandwich bones due to low-velocity projectile impact.

    abstract::Projectile impact in sandwich bones typically results in formation of conoidal wounds exhibiting a larger region of damage on the inner cortical plate termed the bevel. To date, a number of hypotheses have been put forward to explain the formation of this wound type. The plug and spall hypothesis suggests that the con...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Rickman JM,Shackel J

    更新日期:2019-09-01 00:00:00

  • DNA typing of epithelial cells after strangulation.

    abstract::DNA typing was carried out on epithelial cells which were transferred from the hands of the suspect onto the neck of the victim. In an experimental study 16 suspect-victim combinations were investigated for estimating the typing success. Alternatively to an attack against the neck, the upper arm was used for "strangul...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Wiegand P,Kleiber M

    更新日期:1997-01-01 00:00:00

  • How taphonomic alteration affects the detection and imaging of striations in stab wounds.

    abstract::Stabbing with a kitchen knife is a common method of homicide in Europe. Serrated knives may leave tool markings (striations) in tissues. Documentation of striations is necessary for their use as forensic evidence. Traditional methods (physical casting and photography) have significant limitations, and micro-computed t...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Stanley SA,Hainsworth SV,Rutty GN

    更新日期:2018-03-01 00:00:00

  • VWA STR genotyping: further inconsistencies between Perkin-Elmer and Promega kits.

    abstract::We report a simultaneous study of the VWA STR locus by the Perkin-Elmer Profiler Plus kit and the Promega GenePrint CTTv kit in a population sample from North Portugal and in 418 meiosis from family material and paternity cases. PCR amplification and genotyping were performed according to the manufacturer's instructio...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Alves C,Amorim A,Gusmão L,Pereira L

    更新日期:2001-10-01 00:00:00

  • Age estimation from the acetabulum in South African black males.

    abstract::Anthropologists are constantly seeking to improve methods for age estimation in the human skeleton. A new method was introduced about a decade ago that assesses the morphological changes that take place in the acetabulum as an individual ages. The pelvis is usually well preserved in forensic cases, which makes this me...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Botha D,Pretorius S,Myburgh J,Steyn M

    更新日期:2016-05-01 00:00:00

  • Fatality following a suicidal overdose with varenicline.

    abstract::The smoking cessation agent varenicline acts as a partial agonist on α(4)β(2) nicotinic acetylcholine receptors. Although debated, several reports have linked varenicline therapy to an increased risk of suicidal thoughts and/or suicide. In addition, several non-fatal overdose cases have been reported. In this report, ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Stove CP,De Letter EA,Piette MH,Lambert WE

    更新日期:2013-01-01 00:00:00

  • Evaluation of new primers for CSF1PO.

    abstract::We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150-182 bp) than the primers in standard use (295-327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Yoshida K,Sekiguchi K,Kasai K,Sato H,Seta S,Sensabaugh GF

    更新日期:1997-01-01 00:00:00

  • Stability of carbohydrate deficient transferrin (CDT) in stored blood samples.

    abstract::The alcoholism marker CDT was determined on 257 blood samples 1-2 days after the blood samples were taken and again after storage for 7 months at +4 degrees C. The differences between the pairs of CDT values were so large that the determination of CDT after long term storage of the blood sample has no evidential value...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Köhler H,West A,Brinkmann B

    更新日期:2000-01-01 00:00:00

  • Correction to: Age estimation based on pelvic ossification using regression models from conventional radiography.

    abstract::The original version of this article unfortunately contained a mistake in the Results section. The correct equation from the cubic regression analysis for male is: Age = 14.408 + 0.475X-0.064X2 + 0.005X3, and for the female is: 18.196-1.637X + 0.204X2-0.005X3. The error doesn't affect the main conclusion. ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章,已发布勘误


    authors: Zhang K,Dong XA,Fan F,Deng ZH

    更新日期:2018-01-01 00:00:00

  • Fatal sepsis by Klebsiella pneumoniae in a patient with systemic lupus erythematosus: the importance of postmortem microbiological examination for the ex post diagnosis of infection.

    abstract::The utility of postmortem microbiology has continuously been a topic of controversy. The present study describes a case of fatal sepsis in a patient with systemic lupus erythematosus. Postmortem culture and genotyping analyses allowed us to identify Klebsiella pneumoniae as the cause of sepsis, revealing the inadequat...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: D'Ovidio C,Pompilio A,Crocetta V,Gherardi G,Carnevale A,Di Bonaventura G

    更新日期:2015-09-01 00:00:00

  • Determination of latent fingerprint degradation patterns-a real fieldwork study.

    abstract::For over a century, law enforcement agencies, forensic laboratories, and penal courts worldwide have used fingerprint impressions as reliable and conclusive evidence to identify perpetrators of criminal activity. Although fingerprint identification has been repeatedly proven as one of the most robust and definite fore...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: De Alcaraz-Fossoul J,Mestres Patris C,Balaciart Muntaner A,Barrot Feixat C,Gené Badia M

    更新日期:2013-07-01 00:00:00

  • Myeloperoxidase activity in skin lesions. I. Influence of the loss of blood, depth of excoriations and thickness of the skin.

    abstract::The measurement of the myeloperoxidase activity was used to quantify the acute granulocyte reaction. A 35% loss of blood resulted in a clear decrease of the myeloperoxidase activity at the edges of experimental incision wounds in rat skin in the first day after inflicting the wounds. After 12 h only about one-third an...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Laiho K

    更新日期:1998-01-01 00:00:00

  • The iliac crest in forensic age diagnostics: evaluation of the apophyseal ossification in conventional radiography.

    abstract::Due to the increasing significance of forensic age estimations in the age of globalisation, novel radiographic criteria besides clavicles and hand bones may provide additional certainty for forensic age expertises. The present study analyses the suitability of the iliac crest apophysis by means of 643 pelvic radiograp...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Wittschieber D,Vieth V,Domnick C,Pfeiffer H,Schmeling A

    更新日期:2013-03-01 00:00:00

  • Comparison of HPLC and CE for the analysis of dichlorprop in a case of intoxication.

    abstract::A 49-year-old white male was found lying unconscious at home. He had vomited, his mouth was filled with a white foam and a pungent odour filled the room. After emergency treatment blood, urine and stomach contents were screened for drugs after acid and alkaline extraction with subsequent derivatisation and GC-MS analy...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: West A,Frost M,Köhler H

    更新日期:1997-01-01 00:00:00

  • German population data for 18 X-STRs: a hexaplex PCR adding two clusters of X-STRs to the Argus X-12 set and expanding the German haplotype databases.

    abstract::In kinship analysis, large data sets with estimated haplotype frequencies for marker clusters are very important for the likelihood calculation. Practical use of the X-STRs demonstrated that in some complex kinship cases, the marker set of the Investigator Argus X-12 kit can be insufficient. This study aimed to extend...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Hering S,Klimova A,Edelmann J

    更新日期:2020-11-01 00:00:00

  • The role of hereditary KCNQ1 mutations in water-related death.

    abstract::Drowning remains one of the major causes of death in most developed countries despite the fact that many of the victims are known to be at least moderate swimmers as well as healthy directly before the event. Here, fatal arrhythmias and especially the long QT syndrome (LQTS) have been proposed as the underlying mechan...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Tzimas I,Bajanowski T,Poetsch M

    更新日期:2016-03-01 00:00:00

  • Ethyl glucuronide and ethyl sulfate in autopsy samples 27 years after death.

    abstract::The unique case of a 50-year-old known alcoholic whose corpse was exhumed 27 years after death is reported. The man apparently committed suicide by hanging, but many years later the case was questioned and homicide-linked to a long-lasting serial killer case-was suspected. Thus, the corpse was exhumed, and at the auto...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Politi L,Morini L,Mari F,Groppi A,Bertol E

    更新日期:2008-11-01 00:00:00

  • The incidence and position of melanocytic nevi for the purposes of forensic image comparison.

    abstract::Expert witness opinion based on the comparison of images has been accepted by UK courts as admissible evidence in relation to issues of identity. Within images of the hand are a multiplicity of anatomical features of different aetiology, incidence and distribution patterns and this includes melanocytic nevi, referred ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Black S,MacDonald-McMillan B,Mallett X,Rynn C,Jackson G

    更新日期:2014-05-01 00:00:00

  • Evaluation of chronological age based on third molar development in the Spanish population.

    abstract::A cross-sectional study was carried out to assess chronological age estimation based on the stages of third lower molar development, following the eight stages (A-H) method of Demirjian et al. The final sample consisted of 1,054 orthopantomograms from Spanish individuals of known chronological age (range 14-21 years) ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Prieto JL,Barbería E,Ortega R,Magaña C

    更新日期:2005-11-01 00:00:00

  • The pathology of the adrenal glands in sudden infant death syndrome (SIDS)

    abstract::The sudden infant death syndrome (SIDS) is at present based on unknown pathogenetical mechanisms but in industrial nations is the most common cause of death in infancy after the perinatal period. Studies of a large number of adrenal glands in this syndrome have not been reported. Therefore, we evaluated 146 SIDS cases...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章,多中心研究


    authors: Pérez-Platz U,Saeger W,Dhom G,Bajanowski T

    更新日期:1994-01-01 00:00:00

  • How reliable is apparent age at death on cadavers?

    abstract::The assessment of age at death for identification purposes is a frequent and tough challenge for forensic pathologists and anthropologists. Too frequently, visual assessment of age is performed on well-preserved corpses, a method considered subjective and full of pitfalls, but whose level of inadequacy no one has yet ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Amadasi A,Merusi N,Cattaneo C

    更新日期:2015-07-01 00:00:00

  • Genetic data for 26 autosomal STR markers from Brazilian population.

    abstract::The allelic frequency distributions and statistical forensic parameters of 26 mini short tandem repeat (mini-STR) loci in a sample of 1575 unrelated individuals from five different Brazilian regions were obtained. All the analyzed loci showed great diversity and were highly informative. The results were compared with ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Pereira TFC,Malaghini M,Magalhães JCM,Moura-Neto R,Sotomaior VS

    更新日期:2018-09-01 00:00:00

  • Multiple suicidal injuries with shotgun slugs.

    abstract::Shotguns are usually used to fire multiple pellets, but they are capable of firing single projectiles. Shotgun slug injuries are rare, severe, and fully comparable to those inflicted by high-velocity projectiles. A case of gunshot suicide of a 59-year-old man with a shotgun loaded with shotgun slugs is presented. The ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Hejna P

    更新日期:2010-01-01 00:00:00

  • Towards developing forensically relevant single-cell pipelines by incorporating direct-to-PCR extraction: compatibility, signal quality, and allele detection.

    abstract::Current analysis of forensic DNA stains relies on the probabilistic interpretation of bulk-processed samples that represent mixed profiles consisting of an unknown number of potentially partial representations of each contributor. Single-cell methods, in contrast, offer a solution to the forensic DNA mixture problem b...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Sheth N,Swaminathan H,Gonzalez AJ,Duffy KR,Grgicak CM

    更新日期:2021-01-23 00:00:00

  • DNA typing in cases of blood chimerism.

    abstract::A chimera is an organism whose cells derive from two or more distinct zygote lineages. and therefore two different blood cell populations circulate in one individual. To point out the potential pitfalls in forensic analysis, a set of triplets (a girl and two boys) who revealed blood chimerism was investigated with fou...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Milde A,Kühl-Burmeister R,Ritz-Timme S,Kaatsch HJ

    更新日期:1999-01-01 00:00:00