Abstract：
:DNA profiles from degraded samples often suffer from information loss at the longer short tandem repeat (STR) loci. Sensitising the reactions, either by performing additional PCR cycles or increasing the capillary electrophoresis injection settings, carries the risk of over-amplifying or overloading the shorter fragments. We explored whether profiling of degraded DNA can be improved by preferential capturing of the longer amplified fragments. To this aim, a post-PCR purification protocol was developed that is based on AMPure XP beads that have size-selective properties. A comparison was made with an unselective post-PCR purification system (DTR gel filtration) and no purification of the PCR products. Besides a set of differently and serially degraded single source samples, unequal mixtures of degraded DNAs were analysed, in order to extract more genotyping information for the minor contributor without overloading the major component at the shorter amplicons. Purification by the AMPure protocol resulted in higher peak heights especially for the longer amplicons, while DTR gel filtration gave higher peaks for all amplicon sizes. Both purification methods presented more detected alleles, with the AMPure protocol performing slightly better, on average. In conclusion, the in-house developed AMPure protocol can be employed to improve STR profile analysis of degraded single source and (unequally) mixed DNA samples.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Westen AA,van der Gaag KJ,de Knijff P,Sijen Tdoi
10.1007/s00414-012-0816-1subject
Has Abstractpub_date
2013-07-01 00:00:00pages
741-7issue
4eissn
0937-9827issn
1437-1596journal_volume
127pub_type
杂志文章abstract：:The craniofacial superimposition technique is still an important way of identifying skulls when there is no reference sample for a forensic DNA analysis, when DNA typing from remaining tissue samples has failed, or when antemortem dental records are not available. Through the widespread use of digital photography, the...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0494-9
更新日期：2010-09-01 00:00:00
abstract：:The measurement of the myeloperoxidase activity was used to quantify the acute granulocyte reaction. A 35% loss of blood resulted in a clear decrease of the myeloperoxidase activity at the edges of experimental incision wounds in rat skin in the first day after inflicting the wounds. After 12 h only about one-third an...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050101
更新日期：1998-01-01 00:00:00
abstract：:The yaw angle in flight of high velocity bullets has been discussed for a number of years, due to its contribution to the tumbling of the bullet in the target. Since few unclassified reports of contemporary bullets are available, we investigated the yaw angles of the bullets used in the M16A2, G3, AK-47 and AK-74 rifl...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225601
更新日期：1994-01-01 00:00:00
abstract：:A method has been developed for the simultaneous determination of lysergic acid diethylamide (LSD), its epimer iso-LSD, and its main metabolites nor-LSD and 2-oxo-3-hydroxy LSD in blood, urine, and, for the first time, vitreous humor samples. The method is based on liquid/liquid extraction and liquid chromatography-mu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0078-x
更新日期：2007-07-01 00:00:00
abstract：:One of the most fundamental issues in forensic anthropology is the determination of sex and population affinity based on various skeletal elements. Therefore, we compared the sexual dimorphism of the upper facial skeleton from a recent Czech population (twenty-first century) with that of a population from Early Modern...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1625-3
更新日期：2018-01-01 00:00:00
abstract：:A short tandem repeat at the D1S1656 locus was sequenced in 45 selected alleles and 13 different alleles were found which were designated according to the total number of repeats. This STR is a compound hypervariable STR consisting of blocks of (TAGR) repeats with a basic sequence structure (TAGA)4(TGA)0-1(TAGA)6-16(T...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050161
更新日期：1998-01-01 00:00:00
abstract：:Various aspects concerning the practical application and forensic interpretation of data obtained by saliva drug testing and drug monitoring from the skin surface are discussed. Basic information on the composition of saliva and skin secretions and their particular transport mechanisms, as far as known, are given. For...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s004140050239
更新日期：1999-01-01 00:00:00
abstract：:In cooperation with the Crime Investigation Agency (Landeskriminalamt) of North Rhine-Westphalia, we carried out a study of 150 cases of (suspected) neonaticide dating from 1993 to 2007 from all over Germany. The autopsy reports and additional expert opinions (if performed) were evaluated for a minimum of 78 variables...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0841-8
更新日期：2013-05-01 00:00:00
abstract：:Kinship relation and, in particular, paternity probability estimation using a Bayesian approach require the input of a priori probabilities of different hypotheses. In practical case work, a priori probabilities or priors, for short, must often be estimated using only common sense and symmetry arguments because in mos...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0827-6
更新日期：2013-11-01 00:00:00
abstract：:Allele frequencies for 15 autosomal STR loci (N = 290) and haplotype data for 17 Y-STR loci (N = 157) were determined for an admixed population from Belize. There were no detectable departures from Hardy-Weinberg equilibrium expectations at any autosomal STR loci except for the D8S1179 locus (p = 0.002). The combined ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1082-1
更新日期：2015-11-01 00:00:00
abstract：AIM:The aim of the current study was to retrospectively collect dental panoramic radiographs from Somali children living in Finland, to use the radiographic data to develop a new age estimation model based on the model established by Willems et al. (J Forensic Sci 46(4):893-895, 2001), and to compare the age prediction...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1926-1
更新日期：2018-11-01 00:00:00
abstract：:The ability to estimate stature from various parts of the body is essential in medicolegal investigations, particularly in cases in which human remains have been dismembered or mutilated. Therefore, the aim of this study was to generate linear regression models for predicting stature on the basis of sternum length in ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-0975-3
更新日期：2014-09-01 00:00:00
abstract：:In wounds that are inflicted at least 60 min before death, histamine levels can increase up to 100%. This functional effect might have a morphometric counterpart. Mast cells play a crucial role in acute inflammatory reactions and in the healing process of wounds. Therefore, the density of these cells was immunohistoch...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-002-0304-0
更新日期：2003-02-01 00:00:00
abstract：:As a contribution to the geographic coverage of EMPOP, currently the best available forensic mitochondrial DNA (mtDNA) database, a total of 299 Swedish individuals were analysed by sequencing of the first and second hypervariable regions of the mtDNA genome. In this sample set, a total of 179 different haplotypes were...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0908-6
更新日期：2013-11-01 00:00:00
abstract：:We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up....
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0223-9
更新日期：2008-05-01 00:00:00
abstract：:We measured the quantity of D-aspartic acid (degree of racemization of aspartic acid) in the enamel of different types of teeth from the same individual. We studied the correlation between the degree of racemization and the time of formation of each particular tooth, as well as the applicability of the degree of racem...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-004-0506-8
更新日期：2005-03-01 00:00:00
abstract：:The in vitro experiments on the interaction of 3H-cocaine and melanin from Sepia officinalis confirmed the existence of drug binding sites on melanin granules. The results suggested that the binding of 3H-cocaine to melanin could be analyzed by assuming that the binding to the surface of pigment granules is analogous ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050031
更新日期：1997-01-01 00:00:00
abstract：:Chumbinho is the popular name given to carbamate aldicarb (Temik), an insecticide commonly used in agriculture and highly toxic (LD50 = 0.9 mg/kg oral in rats) that has been sold clandestinely in several regions of Brazil. Chumbinho is sold illegally as raticide and is available in a formulation of small black granule...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1336-1
更新日期：2016-07-01 00:00:00
abstract：:Age estimation in living individuals around the age of 18 years remains a difficult challenge. In this study, the anterior inferior vertebral ring apophysis development of cervical vertebrae C2, C3, and C4 of 496 white and 478 black South African individuals aged between 15 and 22 years was assessed from cephalometric...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02137-7
更新日期：2019-11-01 00:00:00
abstract：:Necrophagous blow fly larvae can provide accurate estimates of the minimum postmortem interval in death investigations. During larval development, predictable morphological changes occur and measurements of weight, length, and width are compared to species-specific growth curves for reliable age estimates. However, ag...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0699-1
更新日期：2013-01-01 00:00:00
abstract：:Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which recognize the pericentromeric regions DXZ1 and DYZ1/DYZ3 of the X...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225497
更新日期：1994-01-01 00:00:00
abstract：:Diagnosis of ischaemia-related sudden cardiac death in the absence of microscopic and macroscopic ischaemic lesions remains a challenge for medical examiners. Medical imaging techniques increasingly provide support in post-mortem examinations by detecting and documenting internal findings prior to autopsy. Previous st...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02211-0
更新日期：2020-03-01 00:00:00
abstract：:Forensic age estimation of living individuals has become increasingly important in forensic practice with the constant increase of migration movements to developed countries. The method of Greulich and Pyle is one of the most frequently used methods for age determination. The aim of our study was to verify the reliabi...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1028-7
更新日期：2015-01-01 00:00:00
abstract：:In kinship analysis, large data sets with estimated haplotype frequencies for marker clusters are very important for the likelihood calculation. Practical use of the X-STRs demonstrated that in some complex kinship cases, the marker set of the Investigator Argus X-12 kit can be insufficient. This study aimed to extend...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02306-z
更新日期：2020-11-01 00:00:00
abstract：:The aim of this paper is twofold: first, to evaluate an Indian sample by Cameriere's European formula; and second, if this formula turns out to be unsuitable, to study a specific formula for Indian children. Orthopantomographs taken from 480 Indian children (227 girls and 253 boys) aged between 3 and 15 years were ana...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0427-7
更新日期：2010-05-01 00:00:00
abstract：INTRODUCTION:Electronic control with the CEW (conducted electrical weapon) has gained widespread acceptance as the preferred force option due to its significant injury reduction. However, a CEW application does stress the human body. In the case of the CEW, the human body response is similar to the challenge of physica...
journal_title：International journal of legal medicine
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00414-018-1771-2
更新日期：2018-09-01 00:00:00
abstract：:Allele frequency distributions for 15 tetrameric short tandem repeat (STR) loci were determined using the AmpFlSTR® Identifiler Plus™ PCR amplification kit. There was little evidence of departures from Hardy-Weinberg equilibrium or association of alleles of different loci in the population samples. The probability of ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0898-4
更新日期：2014-03-01 00:00:00
abstract：:This study reports mtDNA polymorphisms in both hypervariable segments HV1 and HV2 of the non coding D-loop region from 60 unrelated Koreans. In contrast to two previous Korean data base studies, mtDNA was extracted separately from pulp tissue and root dentin of teeth obtained from dentists. Dentin turned out to be a r...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050177
更新日期：1998-01-01 00:00:00
abstract：:In many forensic cases, the existence of a traumatic brain injury (TBI) is an essential factor, and the determination of the survival time is nearly as important as the determination of whether or not a trauma exists. Since it is known that glucose uptake increases in injured brain cells in order to perpetuate the neu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1893-6
更新日期：2019-07-01 00:00:00
abstract：:From sequence database information, we have identified two male-specific and polymorphic tetranucleotide STRs, DYS 441 (GDB:10013873) and DYS 442 (GDB: 10030304), on the Y chromosome. Analysis of 184 males allowed 7 and 5 alleles to be distinguished in the DYS 441 and DYS 442 systems, respectively, yielding 21 haploty...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100207
更新日期：2001-08-01 00:00:00
