A comparison of 3H-cocaine binding on melanin granules and human hair in vitro.


:The in vitro experiments on the interaction of 3H-cocaine and melanin from Sepia officinalis confirmed the existence of drug binding sites on melanin granules. The results suggested that the binding of 3H-cocaine to melanin could be analyzed by assuming that the binding to the surface of pigment granules is analogous to the adsorption of a drug on a solid and follows Langmuir adsorption isotherm type I. Scatchard analysis indicated heterogeneity of binding sites. Structural and chemical alterations caused by isolation of the melanoproteins, which are heterogeneous in nature and show different physico-chemical properties, are considered to be most crucial. The studies on hair samples confirmed that melanin-drug interaction occur on the surface of melanin granules. These seem to be of minor importance compared to the drug-melanoprotein loading during melanogenesis for the observed influence of pigmentation on the drug content of hair fibers. From the results it was concluded that in vitro studies on melanin provide limited information and even drug-soaked hair must be regarded as inappropriate for the study of melanin-drug-binding in hair.


Int J Legal Med


Pötsch L,Skopp G,Rippin G




Has Abstract


1997-01-01 00:00:00












  • The application of dental methods of identification to human burn victims in a mass disaster.

    abstract::This paper deals with the usefulness of dental identification in the case of 28 human burn victims of a bus accident in Spain. Postmortem forensic procedures for identification were used including general external examination, routine photographs and radiographs and complementary biological methods. Dental identificat...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Valenzuela A,Martin-de las Heras S,Marques T,Exposito N,Bohoyo JM

    更新日期:2000-01-01 00:00:00

  • Rare cause of natural death in forensic setting: hemophagocytic syndrome.

    abstract::We report about the case of a sudden unexpected death of a 25-year-old male suffering from infectious disease. An autopsy was ordered with no final premortem diagnosis. Microscopic and microbiological examination revealed a pneumococcal bronchopneumonia and hemophagocytic lesions in the bone marrow. After integrating ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Ondruschka B,Habeck JO,Hädrich C,Dreßler J,Bayer R

    更新日期:2016-05-01 00:00:00

  • Backspatter from experimental close-range shots to the head. I. Macrobackspatter.

    abstract::Backspatter is the ejection of biological material from a gunshot entrance wound against the line of fire. This phenomenon was investigated experimentally in transverse gunshots to the heads of calves (n = 9) using two types of 9 mm Parabellum ammunition from shooting distances of 0-10 cm. The resulting bloodstains we...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Karger B,Nüsse R,Schroeder G,Wüstenbecker S,Brinkmann B

    更新日期:1996-01-01 00:00:00

  • Short tandem repeat HumACTBP2 (SE33) and HumVWA: population genetic study on a north Italian population.

    abstract::Allele frequencies at the short tandem repeat (STR) loci HumACTBP2 and HumVWA were determined in 118 unrelated individuals from Northern Italy (Milan area). For locus HumACTBP2 (SE33) a total of 39 alleles was observed. Furthermore, two interalleles (N18m+N19m) and one allele (> N35) were found which were not observed...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Piccinini A,Waterkamp K,Meyer E

    更新日期:1997-01-01 00:00:00

  • Postmortem interrogation of cardiac implantable electrical devices may clarify time and cause of death.

    abstract:BACKGROUND:Postmortem interrogation of cardiac implantable electrical devices (CIED) in autopsy is not routinely performed. Thus, it remains unclear whether an interrogation might clarify time and cause of death. METHODS:Seventy of 4401 patients (1.6%) undergoing autopsy in 2014 and 2015 presented with a CIED. The exp...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Riesinger L,Fichtner S,Schuhmann CG,Estner HL,Czermak T,Graw M,Fischer F,Lackermair K

    更新日期:2019-05-01 00:00:00

  • Molecular characterisation of the nucleic acids recovered from aged forensic samples.

    abstract::The molecular composition of the genetic substrate recovered from seven aged forensic samples has been extensively investigated. A simple enzymatic test based on DNAseI incubation of the extracts showed that the UV-fluorescent material from the forensic specimens is composed of nucleic acids, with the DNA fraction rep...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Previderè C,Micheletti P,Perossa R,Grignani P,Fattorini P

    更新日期:2002-12-01 00:00:00

  • Selection of highly informative SNP markers for population affiliation of major US populations.

    abstract::Ancestry informative markers (AIMs) can be used to detect and adjust for population stratification and predict the ancestry of the source of an evidence sample. Autosomal single nucleotide polymorphisms (SNPs) are the best candidates for AIMs. It is essential to identify the most informative AIM SNPs across relevant p...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Zeng X,Chakraborty R,King JL,LaRue B,Moura-Neto RS,Budowle B

    更新日期:2016-03-01 00:00:00

  • Differences in sampling techniques on total post-mortem tryptase.

    abstract:BACKGROUND:The measurement of mast cell tryptase is commonly used to support the diagnosis of anaphylaxis. In the post-mortem setting, the literature recommends sampling from peripheral blood sources (femoral blood) but does not specify the exact sampling technique. Sampling techniques vary between pathologists, and it...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Tse R,Garland J,Kesha K,Elstub H,Cala AD,Ahn Y,Stables S,Palmiere C

    更新日期:2018-05-01 00:00:00

  • Single nucleotide polymorphisms over the entire mtDNA genome that increase the power of forensic testing in Caucasians.

    abstract::We have sequenced the entire mtDNA genome (mtGenome) of 241 individuals who match 1 of 18 common European Caucasian HV1/HV2 types, to identify sites that permit additional forensic discrimination. We found that over the entire mtGenome even individuals with the same HV1/HV2 type rarely match. Restricting attention to ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Coble MD,Just RS,O'Callaghan JE,Letmanyi IH,Peterson CT,Irwin JA,Parsons TJ

    更新日期:2004-06-01 00:00:00

  • Carisoprodol intoxications: a retrospective study of forensic autopsy material from 1992-2003.

    abstract::Carisoprodol is commonly prescribed as a centrally acting muscle relaxant, but it is also subject to abuse. The literature describing fatal intoxications with the drug is limited to a relatively small number of cases, and there are inconsistencies with regard to which concentration levels that are toxic. We therefore ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Høiseth G,Bramness JG,Christophersen AS,Mørland J

    更新日期:2007-09-01 00:00:00

  • Fatality following a suicidal overdose with varenicline.

    abstract::The smoking cessation agent varenicline acts as a partial agonist on α(4)β(2) nicotinic acetylcholine receptors. Although debated, several reports have linked varenicline therapy to an increased risk of suicidal thoughts and/or suicide. In addition, several non-fatal overdose cases have been reported. In this report, ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Stove CP,De Letter EA,Piette MH,Lambert WE

    更新日期:2013-01-01 00:00:00

  • Population genetic analysis of the Globalfiler STR loci in 3032 individuals from the Altay Han population of Xinjiang in northwest China.

    abstract::The genetic polymorphisms of 21 autosomal short tandem repeat (STR) loci included in the GlobalFiler™ PCR Amplification Kit were evaluated in 3032 unrelated individuals Altay Han of Xinjiang, northwest China. All of the loci reached the Hardy-Weinberg equilibrium. These loci were examined to determine allele frequenci...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Li X,Li L,Wang Q,Zhang J,Ge W,Bai R,Yu X,Shi M

    更新日期:2018-01-01 00:00:00

  • Racemization in enamel among different types of teeth from the same individual.

    abstract::We measured the quantity of D-aspartic acid (degree of racemization of aspartic acid) in the enamel of different types of teeth from the same individual. We studied the correlation between the degree of racemization and the time of formation of each particular tooth, as well as the applicability of the degree of racem...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Ohtani S,Ito R,Arany S,Yamamoto T

    更新日期:2005-03-01 00:00:00

  • How taphonomic alteration affects the detection and imaging of striations in stab wounds.

    abstract::Stabbing with a kitchen knife is a common method of homicide in Europe. Serrated knives may leave tool markings (striations) in tissues. Documentation of striations is necessary for their use as forensic evidence. Traditional methods (physical casting and photography) have significant limitations, and micro-computed t...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Stanley SA,Hainsworth SV,Rutty GN

    更新日期:2018-03-01 00:00:00

  • Pulmonary myelomonocyte subtypes in drowning and other causes of death.

    abstract::Three immunohistochemically different myelomonocytic subtypes, i.e. MRP8, MRP14 and 27E10 were quantitatively evaluated in the intraalveolar, alveolar-interstitial and alveolar-intracapillary lung compartments. Lung sections from 5 major groups with defined causes of death, i.e. drowning and death during immersion (DI...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Brinkmann B,Hernandez MA,Karger B,Ortmann C

    更新日期:1997-01-01 00:00:00

  • Application of ELISA-ABC method to the identification of minute human bloodstains.

    abstract::Bloodstained threads (1 cm in length) were tested to identify human origin by a direct ELISA-ABC method using biotinylated antibody against human HbA0. By this method human bloodstains were clearly distinguishable from bloodstains of other species including Japanese monkey. The minimum detection limit of bloodstains p...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Tokiwa K,Tajima M,Nishina M,Katsura S

    更新日期:1991-01-01 00:00:00

  • First Polish DNA "manhunt"--an application of Y-chromosome STRs.

    abstract::This study presents the application of Y-chromosomal STR polymorphisms to male identification in the case of a serial rapist and woman murderer in Poland. Since August 1996 a rapist from Swinoujscie (northwest Poland) committed at least 14 rapes. In the year 2000 he brutally raped 8 young girls and murdered a 22-year-...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Dettlaff-Kakol A,Pawlowski R

    更新日期:2002-10-01 00:00:00

  • Influence of dose-death interval on colchicine and metabolite distribution in decomposed skeletal tissues.

    abstract::The semi-quantitative analysis of decomposed bone of rats exposed to colchicine and euthanized following different time intervals postexposure (i.e., dose-death interval, DDI) is described. Rats received colchicine (50 mg/kg, i.p.) and were euthanized 30 min (DDI1; n = 4), 60 min (DDI2; n = 4), or 180 min (DDI3; n = 4...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Imfeld AB,Watterson JH

    更新日期:2016-03-01 00:00:00

  • The pathology of the adrenal glands in sudden infant death syndrome (SIDS)

    abstract::The sudden infant death syndrome (SIDS) is at present based on unknown pathogenetical mechanisms but in industrial nations is the most common cause of death in infancy after the perinatal period. Studies of a large number of adrenal glands in this syndrome have not been reported. Therefore, we evaluated 146 SIDS cases...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章,多中心研究


    authors: Pérez-Platz U,Saeger W,Dhom G,Bajanowski T

    更新日期:1994-01-01 00:00:00

  • Improved analysis of long STR amplicons from degraded single source and mixed DNA.

    abstract::DNA profiles from degraded samples often suffer from information loss at the longer short tandem repeat (STR) loci. Sensitising the reactions, either by performing additional PCR cycles or increasing the capillary electrophoresis injection settings, carries the risk of over-amplifying or overloading the shorter fragme...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Westen AA,van der Gaag KJ,de Knijff P,Sijen T

    更新日期:2013-07-01 00:00:00

  • Phylogenetic classification of Japanese mtDNA assisted by complete mitochondrial DNA sequences.

    abstract::We investigated control and coding region polymorphisms in mitochondrial DNA (mtDNA) in 100 unrelated individuals from a Japanese population and determined the basal phylogenetic haplogroup lineages in all samples under updated information. Many of the basal phylogenetic haplogroup lineages assigned on East Asian mtDN...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Nohira C,Maruyama S,Minaguchi K

    更新日期:2010-01-01 00:00:00

  • Technical note: EnVision™ FLEX improves the detectability of depletions of myoglobin and troponin T in forensic cases of myocardial ischemia/infarction.

    abstract::Immunohistochemistry is a well-established technique used in many research laboratories as well as in clinical diagnostics. The method allows to visualize the expression of proteins in biological tissues, as well as to evaluate this expression semi-quantitatively. For diagnosis, an optimal staining, based on a straigh...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Sabatasso S,Pomponio C,Fracasso T

    更新日期:2017-11-01 00:00:00

  • Third molar maturity index (I3M) for assessing age of majority in northern Chinese population.

    abstract::In many countries, assessment of legal age, also known as age of majority, has become increasingly important over the years. In China, individuals older than 18 years of age have full capacity regarding civil conduct and can be tried as an adult for criminal charges. Therefore, from a legal point of view, it is crucia...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Chu G,Wang YH,Li MJ,Han MQ,Zhang ZY,Chen T,Zhou H,Guo YC

    更新日期:2018-11-01 00:00:00

  • Genotyping of 75 SNPs using arrays for individual identification in five population groups.

    abstract::Single nucleotide polymorphism (SNP) typing offers promise to forensic genetics. Various strategies and panels for analyzing SNP markers for individual identification have been published. However, the best panels with fewer identity SNPs for all major population groups are still under discussion. This study aimed to f...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Hwa HL,Wu LS,Lin CY,Huang TY,Yin HI,Tseng LH,Lee JC

    更新日期:2016-01-01 00:00:00

  • Using bone marrow matrix to analyze meprobamate for forensic toxicological purposes.

    abstract::Bone marrow (BM) analysis is of forensic interest for postmortem toxicological investigations where blood samples are unavailable or unusable. Due to the lack of studies, it remains difficult to interpret concentrations of xenobiotics measured in this matrix. Based on a statistical approach published previously to int...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Bévalot F,Gustin MP,Cartiser N,Gaillard Y,Le Meur C,Fanton L,Guitton J,Malicier D

    更新日期:2013-09-01 00:00:00

  • Lethal thyroid storm after uncontrolled intake of liothyronine in order to lose weight.

    abstract::Thyroid hormones are sometimes used for purposes for which they are not approved. Reasons for off-label use can be overweight, prevailing depressive mood, or various somatic symptoms. Information about the intake of thyroid hormones in order to lose weight can be easily obtained from inappropriate/nonmedical websites....

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Hartung B,Schott M,Daldrup T,Ritz-Timme S

    更新日期:2010-11-01 00:00:00

  • Death by chumbinho: aldicarb intoxication-regarding a corpse in decomposition.

    abstract::Chumbinho is the popular name given to carbamate aldicarb (Temik), an insecticide commonly used in agriculture and highly toxic (LD50 = 0.9 mg/kg oral in rats) that has been sold clandestinely in several regions of Brazil. Chumbinho is sold illegally as raticide and is available in a formulation of small black granule...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Durão C,Machado MP

    更新日期:2016-07-01 00:00:00

  • Detection of vaginal fluid stains on common substrates via ATR FT-IR spectroscopy.

    abstract::The analysis of body fluids is of utmost importance in forensic casework since many biological fluids contain DNA. The ATR FT-IR spectroscopy is an emerging approach for the confirmatory, rapid, facile, non-destructive, and on-site identification and differentiation of body fluid stains. Notwithstanding the ATR FT-IR ...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Sharma S,Singh R

    更新日期:2020-09-01 00:00:00

  • Updating the risk profile of fatal head trauma: an autopsy study with focus on age- and sex-dependent differences.

    abstract::Fatal head trauma (FHT) represents one of the most frequent causes of death diagnosed in forensic pathology. However, profound statistic autopsy data on FHT is still sparse. Therefore, the purpose of this study was to investigate the circumstances and injury patterns of FHT with particular focus on age and sex, and ad...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Kronsbein K,Karger B,Budczies J,Pfeiffer H,Wittschieber D

    更新日期:2020-01-01 00:00:00

  • In vitro study of bacterial degradation of ethyl glucuronide and ethyl sulphate.

    abstract::Recent studies show that ethyl glucuronide (EtG) can be decomposed by bacteria; whilst so far no degradation of ethyl sulphate (EtS) has been observed. In the present study, in vitro experiments with bacterial colonies were performed. Bacteria (Escherichia coli, Klebsiella pneumoniae, Clostridium sordellii) were isola...

    journal_title:International journal of legal medicine

    pub_type: 杂志文章


    authors: Baranowski S,Serr A,Thierauf A,Weinmann W,Grosse Perdekamp M,Wurst FM,Halter CC

    更新日期:2008-09-01 00:00:00