Abstract：
:Finding spermatozoa is of the utmost importance in judicial cases involving both the living and the dead; however, most of literature actually deals with inner genitalia and does not take into consideration the chance of external deposition of semen on skin, which is not rare. In addition, the most advanced microscopic technologies such as scanning electron microscopy (SEM) have not been thoroughly investigated within this specific field of research. This study aims at applying SEM analysis to samples of decomposed skin in order to test its potential in detecting spermatozoa particularly in decomposed cadavers. A sample of skin was obtained at autopsy and divided into two thin strips; one of the samples was used as a negative control. Semen was then taken from a "donor" (with a normal spermiogram) and was spread onto the other skin sample. Every 3 days for the first 15 days (for a total of six samples), a standard slide was prepared from swabs on the treated and control skin and analyzed by standard light microscopy. In addition, every 7 days up to 91 days (3 months circa), a skin sample was taken from the positive and negative control and examined by SEM for a total of 14 samples. Results show that after 12 days, light microscopy failed in detecting spermatozoa, whereas they were still visible up to 84 days by SEM analysis. This study therefore suggests the persistence of sperm structures in time and in decomposing material as well as the possible application of SEM technology to decomposed skin in order to detect semen.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Gibelli D,Mazzarelli D,Rizzi A,Kustermann A,Cattaneo Cdoi
10.1007/s00414-013-0817-8subject
Has Abstractpub_date
2013-09-01 00:00:00pages
975-9issue
5eissn
0937-9827issn
1437-1596journal_volume
127pub_type
杂志文章abstract：:We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150-182 bp) than the primers in standard use (295-327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF02441025
更新日期：1997-01-01 00:00:00
abstract：:In the present study 161 Japanese father/son haplotype transfers in 147 pedigrees were analyzed at 14 Y-STRs with two multiplex PCR-based typing systems. Five isolated single repeat mutations were identified at the DYS389I, DYS439, Y-GATA-H4, DYS389II and DYS391 loci, and a pedigree showing triple alleles at the DYS38...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-003-0422-3
更新日期：2004-06-01 00:00:00
abstract：:The assessment of age at death for identification purposes is a frequent and tough challenge for forensic pathologists and anthropologists. Too frequently, visual assessment of age is performed on well-preserved corpses, a method considered subjective and full of pitfalls, but whose level of inadequacy no one has yet ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1042-9
更新日期：2015-07-01 00:00:00
abstract：:DNA typing of cellular debris from perforating bullets was investigated following shooting experiments. A total of 14 perforating gunshots were fired into 9 calves. PCR typing of tissue fragments was done using bovine-specific primers flanking a 247 bp segment within the bovine lactoglobulin gene. Positive amplificati...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01369787
更新日期：1996-01-01 00:00:00
abstract：:Organophosphorus insecticides, such as parathion-ethyl, quinalphos, chlorpyrifos, chlorfenvinphos or diazinon, are still widely used for pest control on crops. These compounds are extremely toxic to humans, and, even though specific legislation exists that controls the use of these substances, the frequency of toxic a...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-019-02119-9
更新日期：2019-11-01 00:00:00
abstract：:The formation of skull fractures is an important topic in legal medicine. In particular, the influence of boundary conditions is controversially discussed in the literature. A study focusing solely on this aspect was missing. This study aimed to investigate the influence of boundary conditions on the energy threshold ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02023-2
更新日期：2020-03-01 00:00:00
abstract：:Blood, saliva, and semen are some of the forensically most relevant biological stains commonly found at crime scenes, which can often be of small size or challenging due to advanced decay. In this context, it is of great importance to possess reliable knowledge about the effects of degradation under different environm...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1373-9
更新日期：2016-11-01 00:00:00
abstract：:Age estimation in living individuals around the age of 18 years remains a difficult challenge. In this study, the anterior inferior vertebral ring apophysis development of cervical vertebrae C2, C3, and C4 of 496 white and 478 black South African individuals aged between 15 and 22 years was assessed from cephalometric...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02137-7
更新日期：2019-11-01 00:00:00
abstract：:Determining the time of injury is an important but still a challenging task in forensic anthropology. In literature, many descriptions can be found to make a distinction between perimortem and postmortem fractures. Characteristics that are more related to fractures in fresh conditions, however, are not extensively inv...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1514-1
更新日期：2017-05-01 00:00:00
abstract：:A chimera is an organism whose cells derive from two or more distinct zygote lineages. and therefore two different blood cell populations circulate in one individual. To point out the potential pitfalls in forensic analysis, a set of triplets (a girl and two boys) who revealed blood chimerism was investigated with fou...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050263
更新日期：1999-01-01 00:00:00
abstract：:Genetic data from 21 autosomal insertion-null (INNULs) markers found in the InnoTyper® 21 Human DNA Analysis (InnoGenomics®) was evaluated in 190 unrelated individuals from Andalusia. Allele frequencies and forensic parameters were estimated for the 20 INNULs. All loci were in Hardy-Weinberg equilibrium in the study p...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02044-x
更新日期：2019-09-01 00:00:00
abstract：:The measurement of the myeloperoxidase activity was used to quantify the acute granulocyte reaction. A 35% loss of blood resulted in a clear decrease of the myeloperoxidase activity at the edges of experimental incision wounds in rat skin in the first day after inflicting the wounds. After 12 h only about one-third an...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050101
更新日期：1998-01-01 00:00:00
abstract：:We present a single case report illustrating the diagnostic role of multi-slice computed tomography (MSCT) in the investigation of suspicious child death where mechanical asphyxia is suspected. The case illustrates how radiological findings that may not be observable on conventional plain X-ray were identified by post...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0438-4
更新日期：2010-11-01 00:00:00
abstract：:The systemic distribution of kerosene components in blood and tissues was analysed in rats following dermal exposure. Four types of trimethylbenzenes (TMBs) and aliphatic hydrocarbons (AHCs) with carbon numbers 9-16 (C(9)-C(16)) were analysed as major kerosene components by capillary gas chromatography/mass spectromet...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-001-0282-7
更新日期：2002-08-01 00:00:00
abstract：:The alcoholism marker CDT was determined on 257 blood samples 1-2 days after the blood samples were taken and again after storage for 7 months at +4 degrees C. The differences between the pairs of CDT values were so large that the determination of CDT after long term storage of the blood sample has no evidential value...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050013
更新日期：2000-01-01 00:00:00
abstract：:The 5 tetranucleotide short tandem repeats, HUMTH01, HUMVWA31/A, HUMF13A1, HUMFES/FPS and HUMLPL were studied using different electrophoretic methods and PCR amplification conditions in order to optimize the typing conditions. A genetic population study in the population of Galicia was carried out and the allele and g...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01246874
更新日期：1995-01-01 00:00:00
abstract：:In wounds that are inflicted at least 60 min before death, histamine levels can increase up to 100%. This functional effect might have a morphometric counterpart. Mast cells play a crucial role in acute inflammatory reactions and in the healing process of wounds. Therefore, the density of these cells was immunohistoch...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-002-0304-0
更新日期：2003-02-01 00:00:00
abstract：:A case of rupture of the superior mesenteric vein with hemoretroperitoneum is described. The autopsy revealed the presence of an acute pancreatitis and thrombosis of the superior mesenteric artery. From the histological findings it appeared that the acute pancreatitis had damaged the venous wall from the outside and t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01369553
更新日期：1992-01-01 00:00:00
abstract：:This paper considers the statistical evaluation of DNA mixtures in the following situations: (1) two unknown contributors are related respectively to two typed persons, (2) two of the unknown or untyped contributors are related and the third unknown contributor is related to a typed person, or (3) there are two pairs ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-004-0493-9
更新日期：2005-09-01 00:00:00
abstract：:A publically available database of the most highly cited scientists in all disciplines was used to identify people that belonged to the subject category "forensic science and legal medicine." This bibliometric information was derived from Elsevier's SCOPUS database containing eight million scientists with at least fiv...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02491-x
更新日期：2021-01-02 00:00:00
abstract：:Allele frequencies for 15 autosomal STR loci (N = 290) and haplotype data for 17 Y-STR loci (N = 157) were determined for an admixed population from Belize. There were no detectable departures from Hardy-Weinberg equilibrium expectations at any autosomal STR loci except for the D8S1179 locus (p = 0.002). The combined ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1082-1
更新日期：2015-11-01 00:00:00
abstract：:Stabbing with a kitchen knife is a common method of homicide in Europe. Serrated knives may leave tool markings (striations) in tissues. Documentation of striations is necessary for their use as forensic evidence. Traditional methods (physical casting and photography) have significant limitations, and micro-computed t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1715-2
更新日期：2018-03-01 00:00:00
abstract：:The development rate of immature Calliphora vicina reared on decomposed liver was significantly slower, by as much as 30 h (55.4 % of total development time) for mid-sized larvae, and 71 h (35.0 %) and 58 h (14.6 %) if using times to the onset of pupariation and eclosion, respectively, than those of immatures that dev...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0697-3
更新日期：2013-01-01 00:00:00
abstract：:In kinship analysis, large data sets with estimated haplotype frequencies for marker clusters are very important for the likelihood calculation. Practical use of the X-STRs demonstrated that in some complex kinship cases, the marker set of the Investigator Argus X-12 kit can be insufficient. This study aimed to extend...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02306-z
更新日期：2020-11-01 00:00:00
abstract：:We demonstrate the applicability of ion-pair reversed-phase high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry (ICEMS) for the simultaneous characterization of length and nucleotide polymorphisms. Two sections within the first (HVS-I) and second (HVS-II) hypervariable segme...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0117-7
更新日期：2007-01-01 00:00:00
abstract：:The authors report on a young boy who was bitten into his face by an unknown animal while being asleep in a tent. Given the bite marks and the location of the scene, members of the mustelidae and canidae families were the first "suspects." Deoxyribunucleic acid (DNA) recovered from the tent's wall was analyzed with re...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0674-x
更新日期：2012-05-01 00:00:00
abstract：:Reports on penetrating ballistic head injuries in the literature are dominated by case studies of suicides; the penetrating ammunition usually being .22 rimfire or shotgun. The dominating cause of injuries in modern warfare is fragmentation and hence, this is the primary threat that military helmets protect the brain ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1073-2
更新日期：2015-05-01 00:00:00
abstract：:For a long time, it has been thought that fresh and frozen tissues are the only possible source of biological material useful to extract nucleic acids suitable for downstream molecular analysis. Recently, for forensic purpose such as personal identification, also fixed tissues have been used to recover DNA molecules, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1066-1
更新日期：2015-01-01 00:00:00
abstract：:The semi-quantitative analysis of decomposed bone of rats exposed to colchicine and euthanized following different time intervals postexposure (i.e., dose-death interval, DDI) is described. Rats received colchicine (50 mg/kg, i.p.) and were euthanized 30 min (DDI1; n = 4), 60 min (DDI2; n = 4), or 180 min (DDI3; n = 4...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1196-0
更新日期：2016-03-01 00:00:00
abstract：:We analysed 15 autosomal STRs in 200 unrelated individuals (102 males and 98 females) and 17 Y-STRs in 102 unrelated males living in Jharkhand, India, to establish parameters of forensic interest. The examined autosomal STRs revealed high combined power of exclusion (CPE) and combined power of discrimination (CPD) as ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1610-x
更新日期：2018-01-01 00:00:00
