Abstract：
:We demonstrate the applicability of ion-pair reversed-phase high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry (ICEMS) for the simultaneous characterization of length and nucleotide polymorphisms. Two sections within the first (HVS-I) and second (HVS-II) hypervariable segments of the mitochondrial (mt)DNA control region were selected as targets, both containing poly-cytosine (C) tracts, which display length heteroplasmy at a substantial frequency in the population. The two mtDNA sections were simultaneously amplified and analyzed by ICEMS in 90 maternally unrelated mother-offspring pairs from Austria. The findings were confirmed by direct sequencing of the polymerase chain reaction products. For the detailed characterization of present-length heteroplasmic variants, the results retrieved through ICEMS were more informative compared with those derived from direct sequencing. Hence, ICEMS represents an interesting option for successful application in forensic science.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Oberacher H,Niederstätter H,Parson Wdoi
10.1007/s00414-006-0117-7subject
Has Abstractpub_date
2007-01-01 00:00:00pages
57-67issue
1eissn
0937-9827issn
1437-1596journal_volume
121pub_type
杂志文章abstract：:The analysis of a mixed biological stain by means of highly polymorphic VNTR systems usually reveals a profile composed of multiple alleles. If the victim and one or several suspects match the profile the evidential strength of the matches has to be very carefully analysed. The appropriate methods for the statistical ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050019
更新日期：1999-01-01 00:00:00
abstract：:Allele frequencies and forensic parameters for 17 autosomal short tandem repeat (STR) loci were investigated from 1542 individuals of Va ethnic minority in Yunnan Province in the southwest of China. Also, genetic distances between Va and 13 published populations were compared, and a neighbor-joining (NJ) phylogenetic ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1620-8
更新日期：2017-09-01 00:00:00
abstract：:In certain circumstances, tumour tissue specimens are the only DNA resource available for forensic DNA analysis. However, cancer tissues can show microsatellite instability and loss of heterozygosity which, if concerning the short tandem repeats (STRs) used in the forensic field, can cause misinterpretation of the res...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0443-7
更新日期：2011-05-01 00:00:00
abstract：:ABO blood grouping of human bloodstains was performed by a sandwich ELISA using a species-specific monoclonal antibody to the amino-terminal cytoplasmic domain of human red cell membrane band 3. In a blind trial, all A, B and O bloodstains (a 1 cm long thread) and AB bloodstains (a 1.5 cm long thread) were accurately ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01642795
更新日期：1993-01-01 00:00:00
abstract：:Biological samples, including skeletal remains exposed to environmental insults for extended periods of time, exhibit increasing levels of DNA damage and fragmentation. Human forensic identification methods typically use a combination of mitochondrial (mt) DNA sequencing and short tandem repeat (STR) analysis, which t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02110-4
更新日期：2019-09-01 00:00:00
abstract：:Due to the requirement to minimise exposure to radiation, it is desirable to develop non-ionising imaging procedures for the analysis of skeletal maturation for forensic age diagnostics in living individuals. The present pilot study analyses the applicability of ultrasound examinations for the evaluation of apophyseal...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-011-0554-9
更新日期：2011-03-01 00:00:00
abstract：:The original version of this article contained an author name error. In this article, Katrina Madella has been corrected to Katrina Maddela. ...
journal_title：International journal of legal medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00414-019-02031-2
更新日期：2019-05-01 00:00:00
abstract：:Allele frequencies and forensic statistics of 13 autosomal short tandem repeat loci were estimated in 56 unrelated Iranian individuals. Except for two loci which were monomorph, a total of 5-11 alleles at each locus were observed and altogether 94 alleles for all selected loci were found. Our results show that 11 out ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1342-3
更新日期：2016-11-01 00:00:00
abstract：:Linking biological samples found at a crime scene with the actual crime event represents the most important aspect of forensic investigation, together with the identification of the sample donor. While DNA profiling is well established for donor identification, no reliable methods exist for timing forensic samples. He...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0457-1
更新日期：2010-09-01 00:00:00
abstract：:Mitragyna speciosa (Rubiaceae), commonly known as kratom, is a tropical tree with a long history of traditional use in parts of Africa and Southeast Asia. In recent years, kratom has gained popularity for use as a recreational drug across the globe. Relatively new to the illicit market and used in a manner different f...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-015-1279-y
更新日期：2016-01-01 00:00:00
abstract：:Volatile substances are used widespread, especially among young people, as a cheap and easily accessible drug. Tetrachloroethylene is one of the solvents exerting effects on the central nervous system with experiences of disinhibition and euphoria. The case presented is that of a 27-year-old female, found dead by her ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1143-0
更新日期：2015-05-01 00:00:00
abstract：:In order to establish a reliable age indicator in the period when all other epiphyseal age indicators have already been inactivated, medial clavicle as the bone with the longest period of growth became the object of various investigations. However, the lack of population-specific method often made it unreliable in som...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0791-6
更新日期：2013-05-01 00:00:00
abstract：:Alleles of the STR systems HumFES/FPS, HumVWA and HumD21S11 were sequenced and analyzed. Sequence data revealed 3 different systems concerning the complexity of their sequence structure. HumFES/FPS belongs to the STR polymorphism with a simple repeat structure. Only 2 subtypes were found with a base substitution in th...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01224779
更新日期：1994-01-01 00:00:00
abstract：:The yaw angle in flight of high velocity bullets has been discussed for a number of years, due to its contribution to the tumbling of the bullet in the target. Since few unclassified reports of contemporary bullets are available, we investigated the yaw angles of the bullets used in the M16A2, G3, AK-47 and AK-74 rifl...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225601
更新日期：1994-01-01 00:00:00
abstract：:Despite being a common form of abuse, there is a paucity of literature describing shackling and wrist restraint injuries among survivors of torture. Forensic evaluation of alleged wrist restraint/handcuff injuries in survivors of torture presents challenges to the evaluator, especially if the injuries are remote and d...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02451-5
更新日期：2021-01-07 00:00:00
abstract：:The likelihood ratio is the fundamental quantity that summarizes the evidence in forensic cases. Therefore, it is important to understand the theoretical properties of this statistic. This paper is the last in a series of three, and the first to study linked markers. We show that for all non-inbred pairwise kinship co...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1416-2
更新日期：2016-11-01 00:00:00
abstract：:Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which recognize the pericentromeric regions DXZ1 and DYZ1/DYZ3 of the X...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225497
更新日期：1994-01-01 00:00:00
abstract：:Sudden infant death syndrome (SIDS) is a multifactorial syndrome and assumingly, among other mechanisms, a deficit in respiratory control leads to a failure of arousal and autoresuscitation when the child is challenged by a stressful homeostatic event, e.g., hypoxia. We hypothesize that genetic polymorphisms involved ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1232-0
更新日期：2015-09-01 00:00:00
abstract：:Establishing individuality is an imperative aspect in any investigation procedure. At times, it becomes necessary to determine the sex of the individual to establish identity, and saliva stains found at the scene of crime are of major help in such cases. In the present study, we have determined the sex of the individu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0285-8
更新日期：2009-09-01 00:00:00
abstract：:We present a case of fatal intoxication by the application of a transdermal fentanyl patch upon a superficial bleeding abrasion of a 2-year-old girl. The grandmother discovered the body of the child in bed at approximately 7 a.m. External examination revealed a properly developed, nourished, and hydrated child, with s...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1209-z
更新日期：2015-11-01 00:00:00
abstract：:The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise. In this study, we sequenced the mitochondrial cytochrome c oxidase subunit I (COI) gene of 126 specimens of 56 W European Sarcophaga species and added GenBank data to our database to ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0767-6
更新日期：2013-03-01 00:00:00
abstract：:Population genetic studies were carried out on randomly selected and unrelated healthy individuals from Sicily (n = 140-150 individuals) using the short tandem repeat (STR) systems HumTH01, HumvWA31, HumFESFPS and HumF13A01. After vertical electrophoresis on polyacrylamide denaturing gels 6 alleles could be identified...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050171
更新日期：1998-01-01 00:00:00
abstract：:Bullet velocity is a basic parameter in wound ballistics studies. It is usually measured electronically by means of a variety of solid or photoelectric barriers connected to equipment measuring the time elapsing between impulses, enabling the velocity to be calculated. With the advent of Doppler radar velocity measure...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01247266
更新日期：1994-01-01 00:00:00
abstract：:We immunohistochemically examined the intrathrombotic dynamics of autophagy during thrombogenesis using murine deep vein thrombosis (DVT) models. To perform the immunohistochemical analyses, we used anti-LC3 antibody and anti-p62 antibody for detecting the intrathrombotic autophagic functions. We estimated dynamics of...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02168-0
更新日期：2020-05-01 00:00:00
abstract：:Carisoprodol is commonly prescribed as a centrally acting muscle relaxant, but it is also subject to abuse. The literature describing fatal intoxications with the drug is limited to a relatively small number of cases, and there are inconsistencies with regard to which concentration levels that are toxic. We therefore ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0139-1
更新日期：2007-09-01 00:00:00
abstract：:One of the fundamental questions in forensic medicine and anthropology is whether or not a bone or bone fragment is human. Surprisingly at times for the extreme degradation of the bone (charred, old), DNA cannot be successfully performed and one must turn to other methods. Histological analysis at times can be propose...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1781-0
更新日期：2018-09-01 00:00:00
abstract：:Warping has been used to determine the pre-burning condition of human skeletal remains. In the literature, this modification has been associated more often with the burning of fleshed and green bones, but it also arises during the burning of dry bones. The objective of this paper was to assess if bone collagen content...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1400-x
更新日期：2016-11-01 00:00:00
abstract：:Short tandem repeats (STRs) are DNA regions with a variable number of repeated units (2-6 bp) and attractive to genetic application like forensic, population genetics, and anthropological studies. The Huaxia Platinum System allows the multiplex amplification of 23 autosomal STR loci and two gender determination loci. ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1679-2
更新日期：2018-07-01 00:00:00
abstract：:A 28-year-old man was shot in the back of the head at close range by a robber who then locked him in a room assuming that he was dead. The man was discovered 2 days later. The entrance wound of the bullet was in the left occipital region and it passed into the periphery of the right temporal lobe, where it lodged. The...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004149900120
更新日期：2000-01-01 00:00:00
abstract：:For over a century, law enforcement agencies, forensic laboratories, and penal courts worldwide have used fingerprint impressions as reliable and conclusive evidence to identify perpetrators of criminal activity. Although fingerprint identification has been repeatedly proven as one of the most robust and definite fore...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0797-0
更新日期：2013-07-01 00:00:00
