当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF LEGAL MEDICINE期刊下所有文献 > Molecular analysis of different classes of RNA molecules from formalin-fixed paraffin-embedded autoptic tissues: a pilot study.

Molecular analysis of different classes of RNA molecules from formalin-fixed paraffin-embedded autoptic tissues: a pilot study.

Abstract：

:For a long time, it has been thought that fresh and frozen tissues are the only possible source of biological material useful to extract nucleic acids suitable for downstream molecular analysis. Recently, for forensic purpose such as personal identification, also fixed tissues have been used to recover DNA molecules, whereas RNA extracted from such material is still considered too degraded for gene expression studies. In the present pilot study, we evaluated the possibility to use forensic formalin-fixed paraffin-embedded (FFPE) samples, collected at autopsy at different postmortem intervals (PMI) from four individuals, to perform advanced molecular analyses. In particular, we performed qualitative and quantitative analyses of total RNAs extracted from different FFPE tissues and put expression profiles in relation with the organ type and the duration of PMI. Different classes of RNA molecular targets were studied by real-time quantitative RT-PCR. We report molecular evidence that small RNAs are the only RNA molecules still detectable in all the FFPE autoptic tissues. In particular, microRNAs (miRNAs) represent a consistent, stable, and well-preserved molecular target detectable even from tissue sources displaying signs of ongoing putrefaction at autopsy. In this pilot study, we show that miRNAs could represent a highly sensitive and potentially useful forensic marker. Amplification of specific miRNAs using paraffin-embedded blocks could facilitate retrospective molecular analysis using specific forensic-archived tissues chosen as most suitable according to PMI, and this approach would address molecular evidence in forensic cases in which fresh or frozen material is no longer available.

journal_name

Int J Legal Med

journal_title

International journal of legal medicine

authors

Muciaccia B,Vico C,Aromatario M,Fazi F,Cecchi R

doi

10.1007/s00414-014-1066-1

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

11-21

issue

1

eissn

0937-9827

issn

1437-1596

journal_volume

129

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF LEGAL MEDICINE文献大全
  • An acute gabapentin fatality: a case report with postmortem concentrations.

    abstract：:Gabapentin (GBP) (Neurontin®, Horizant®, Gralise®) is a widely prescribed medication used primarily for the treatment of epilepsy and neuropathic pain. GBP has a favorable adverse effect profile in therapeutic dosing with the most common reported effects being dizziness, fatigue, drowsiness, weight gain, and periphera...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-015-1193-3

    authors： Cantrell FL,Mena O,Gary RD,McIntyre IM

    更新日期：2015-07-01 00:00:00

  • Metal pins fired from unmodified blank cartridge guns and very small calibre weapons--technical and wound ballistic aspects.

    abstract：:Blank cartridge guns are generally regarded as being harmless and are not considered to be firearms in most countries. A comparison of the legal situations in Germany and Austria concerning weapons is given. There have been several reports of serious injuries and even fatalities due to these weapons. Ballistic experim...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004140050156

    authors： Rabl W,Riepert T,Steinlechner M

    更新日期：1998-01-01 00:00:00

  • Fatal poisonings where ethylmorphine from antitussive medications contributed to death.

    abstract：:The hypothesis that antitussives containing ethylmorphine are abused by alcoholics and drug addicts and that this may lead to fatal poisonings where ethylmorphine causes or contributes to death was investigated. For this purpose 14 cases were analysed where a blood ethylmorphine concentration above the therapeutic lev...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004140050253

    authors： Jonasson B,Jonasson U,Holmgren P,Saldeen T

    更新日期：1999-01-01 00:00:00

  • Impact energy of everyday items used for assault.

    abstract：:Blunt force is a frequently used type of violence especially because it can be performed with basically every object of our daily lives or with bare hands or feet. The injuries and medical consequences have been widely examined, whereas the forces and especially the energies acting on impact have rarely been analyzed....

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-017-1689-0

    authors： Siegenthaler L,Sprenger FD,Kneubuehl BP,Jackowski C

    更新日期：2018-01-01 00:00:00

  • Population data of 30 insertion-deletion markers in four Chinese populations.

    abstract：:In this study, we assessed 30 insertion-deletion polymorphisms (Indels) (Investigator DIPplex® kit) in four Chinese populations (n = 952) and evaluated their usefulness in forensic genetic applications. After the Bonferroni correction at a 95 % significance level (p = 0.0017), there were no deviations from the Hardy-W...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1091-0

    authors： Shi M,Liu Y,Bai R,Jiang L,Lv X,Ma S

    更新日期：2015-01-01 00:00:00

  • Humeral development from neonatal period to skeletal maturity--application in age and sex assessment.

    abstract：:The goal of the present study is to examine cross-sectional information on the growth of the humerus based on the analysis of four measurements, namely, diaphyseal length, transversal diameter of the proximal (metaphyseal) end of the shaft, epicondylar breadth and vertical diameter of the head. This analysis was perfo...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-012-0713-7

    authors： Rissech C,López-Costas O,Turbón D

    更新日期：2013-01-01 00:00:00

  • Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?

    abstract：:In humans, the amelogenin gene is present on both the X and the Y chromosomes. However, there are size differences in this gene between these chromosomes, which have been utilised for sexing in forensic casework and prenatal diagnosis. Our study using the AmpFl STR Profiler Plus kit, showed a deletion of Y chromosome-...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-001-0262-y

    authors： Thangaraj K,Reddy AG,Singh L

    更新日期：2002-04-01 00:00:00

  • A new technique for the postmortem detection of tooth-coloured dental restorations.

    abstract：:Identification of unknown bodies is mainly made by dental examination and comparison with accurate dental records. Therefore it is necessary to examine the jaws carefully and to locate every tooth-coloured dental restoration. Overlooking dental fillings can make positive identification impossible. The technique descri...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004140050138

    authors： Benthaus S,DuChesne A,Brinkmann B

    更新日期：1998-01-01 00:00:00

  • Polymorphisms in genes of respiratory control and sudden infant death syndrome.

    abstract：:Sudden infant death syndrome (SIDS) is a multifactorial syndrome and assumingly, among other mechanisms, a deficit in respiratory control leads to a failure of arousal and autoresuscitation when the child is challenged by a stressful homeostatic event, e.g., hypoxia. We hypothesize that genetic polymorphisms involved ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-015-1232-0

    authors： Läer K,Dörk T,Vennemann M,Rothämel T,Klintschar M

    更新日期：2015-09-01 00:00:00

  • Capillary zone electrophoresis and artificial neural networks for estimation of the post-mortem interval (PMI) using electrolytes measurements in human vitreous humour.

    abstract：:Determination of electrolyte concentrations (mainly potassium) in vitreous humour has long been considered an important tool in human death investigations for the estimation of the post-mortem interval (PMI). On the basis of its well known potential in ion analysis, capillary zone electrophoresis (CZE) has recently be...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004140100239

    authors： Bocaz-Beneventi G,Tagliaro F,Bortolotti F,Manetto G,Havel J

    更新日期：2002-02-01 00:00:00

  • Feasibility of an accelerated PVAL method for the collection of GSR and biological traces.

    abstract：:The polyvinyl alcohol method (PVAL) is known as an effective technique to thoroughly collect traces of gunshot residue (GSR) from different surfaces, e.g., from hands or gunshot wounds. Despite obvious advantages over other methods using adhesive tapes, PVAL is still not widely accepted and applied in routine case wor...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-019-02166-2

    authors： Lux C,Taube L,Verhoff MA,Kurscheid S,Zöller-Huse G,Welkerling S,Schumacher R,Neimke D,Kettner M

    更新日期：2020-05-01 00:00:00

  • Superimposition technique for skull identification with Afloat® software.

    abstract：:The craniofacial superimposition technique is still an important way of identifying skulls when there is no reference sample for a forensic DNA analysis, when DNA typing from remaining tissue samples has failed, or when antemortem dental records are not available. Through the widespread use of digital photography, the...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-010-0494-9

    authors： Birngruber CG,Kreutz K,Ramsthaler F,Krähahn J,Verhoff MA

    更新日期：2010-09-01 00:00:00

  • Detainees in police custody in the Paris, France area: medical data and high-risk situations (a prospective study over 1 year).

    abstract：BACKGROUND AND OBJECTIVE:The annual number of detainees held in police custody in France is approximately 700,000. Medical data regarding arrestees are scarce across countries. We present the medical characteristics of detainees kept in police custody, including addictive behaviours and high-risk medical situations. M...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-0990-4

    authors： Chariot P,Beaufrère A,Denis C,Dang C,Vincent R,Boraud C

    更新日期：2014-09-01 00:00:00

  • Higher detection rate of hepatitis G and C virus RNA in liver tissue than in serum of deceased injection drug users.

    abstract：:To examine the prevalence of hepatitis G virus (HGV) and hepatitis C virus (HCV) infections in deceased injection drug users and for comparison of the detection rates of HGV and HCV RNA in liver tissue with detection rates in postmortem serum samples, RT-PCR was performed in 50 drug abuse-related fatalities. HGV RNA w...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004140050195

    authors： Seidl S,Koenig B,Reinhardt G,Hampl W,Mertens T,Michel D

    更新日期：1998-01-01 00:00:00

  • Differences in sampling techniques on total post-mortem tryptase.

    abstract：BACKGROUND:The measurement of mast cell tryptase is commonly used to support the diagnosis of anaphylaxis. In the post-mortem setting, the literature recommends sampling from peripheral blood sources (femoral blood) but does not specify the exact sampling technique. Sampling techniques vary between pathologists, and it...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-017-1738-8

    authors： Tse R,Garland J,Kesha K,Elstub H,Cala AD,Ahn Y,Stables S,Palmiere C

    更新日期：2018-05-01 00:00:00

  • Classifying stages of third molar development: crown length as a predictor for the mature root length.

    abstract：:Multiple tooth development staging techniques were reported based on arbitrarily set borderlines between succeeding stages. Anatomic tooth features or predictions of future tooth part dimensions were described to identify the thresholds between the established stages. The need to predict mature tooth dimensions, while...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1011-3

    authors： Altalie S,Thevissen P,Willems G

    更新日期：2015-01-01 00:00:00

  • European council of legal medicine (ECLM) guidelines for the examination of suspected elder abuse.

    abstract：:Article 25 of the Charter of Fundamental Rights of the European Union (adopted in Nice on 7 December 2000) recognizes and respects the rights of older people to lead a life of dignity and independence and to participate in social and cultural life. It also highlights the importance of prevention and recognition of eld...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章,实务指引

    doi：10.1007/s00414-018-1880-y

    authors： Keller E,Santos C,Cusack D,Väli M,Ferrara D,Ludes B,Mangin P,Payne-James JJ,Vieira DN

    更新日期：2019-01-01 00:00:00

  • The combined evidential value of autosomal and Y-chromosomal DNA profiles obtained from the same sample.

    abstract：:When a Y-chromosomal and a (partial) autosomal DNA profile are obtained from one crime sample, and both profiles match the suspect's profiles, we would like to know the combined evidential value. To calculate the likelihood ratio of observing the autosomal and Y-chromosomal DNA profiles combined, we need to know the c...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-0971-7

    authors： de Zoete J,Sjerps M,Meester R,Cator E

    更新日期：2014-11-01 00:00:00

  • Racemization in enamel among different types of teeth from the same individual.

    abstract：:We measured the quantity of D-aspartic acid (degree of racemization of aspartic acid) in the enamel of different types of teeth from the same individual. We studied the correlation between the degree of racemization and the time of formation of each particular tooth, as well as the applicability of the degree of racem...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-004-0506-8

    authors： Ohtani S,Ito R,Arany S,Yamamoto T

    更新日期：2005-03-01 00:00:00

  • Lethal thyroid storm after uncontrolled intake of liothyronine in order to lose weight.

    abstract：:Thyroid hormones are sometimes used for purposes for which they are not approved. Reasons for off-label use can be overweight, prevailing depressive mood, or various somatic symptoms. Information about the intake of thyroid hormones in order to lose weight can be easily obtained from inappropriate/nonmedical websites....

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-010-0423-y

    authors： Hartung B,Schott M,Daldrup T,Ritz-Timme S

    更新日期：2010-11-01 00:00:00

  • Individual identification of flood victims by DNA polymorphisms and autopsy findings.

    abstract：:On September 23rd 1993 Genova was flooded by heavy rainstorms and 4 people disappeared, including an elderly couple. Four days later a partially skeletonized body was found floating near the coast. No visual identification was possible. Autopsy findings were consistent with the medical history of a possible victim. DN...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/BF01428409

    authors： Mannucci A,Casarino L,Bruni G,Lomi A,De Stefano F

    更新日期：1995-01-01 00:00:00

  • BamHI polymorphism of locus D2S44 in a West German population as revealed by VNTR probe YNH24.

    abstract：:BamHI polymorphism at the VNTR locus D2S44 was investigated, concentrating on band frequencies, mutation rate and confirmation of Mendelian inheritance. In this series 39 restriction fragments showing frequencies less than 10% could clearly be distinguished. No mutations could be observed and the Mendelian character o...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/BF01816481

    authors： Henke L,Cleef S,Zakrzewska M,Henke J

    更新日期：1990-12-01 00:00:00

  • Genetic study of 12 X-STRs in Malay population living in and around Kuala Lumpur using Investigator Argus X-12 kit.

    abstract：:We investigated 12 X-chromosomal short tandem repeat (STR) polymorphisms in 283 unrelated Malay individuals (160 males and 123 females) living in and around Kuala Lumpur using the Investigator Argus X-12 kit. Heterozygosity among the present 12 X-STRs showed a distribution of from 55.3 to 93.5 %. The diversity values ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-012-0705-7

    authors： Samejima M,Nakamura Y,Nambiar P,Minaguchi K

    更新日期：2012-07-01 00:00:00

  • Gunshot wound to the head with full recovery.

    abstract：:A 28-year-old man was shot in the back of the head at close range by a robber who then locked him in a room assuming that he was dead. The man was discovered 2 days later. The entrance wound of the bullet was in the left occipital region and it passed into the periphery of the right temporal lobe, where it lodged. The...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004149900120

    authors： Rothschild MA,Schneider V

    更新日期：2000-01-01 00:00:00

  • Forensic discrimination of three common brands of kitchen knives in China by ICP-AES and infrared absorption.

    abstract：:A block of an injury instrument will be left in wounds sometimes, and the suspect instrument can be discriminated by comparison with the block that was left through elemental analysis. In this study, three brands (Shibazi, Zhangxiaoquan, Qiaoxifu) of kitchen knives with forged, chop, and slice application series were ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-013-0829-4

    authors： Bai RF,Ma SH,Zhang HD,Chang L,Zhang Z,Liu L,Zhang FQ,Guo ZM,Shi MS

    更新日期：2014-03-01 00:00:00

  • A proposed nomenclature for 15 canine-specific polymorphic STR loci for forensic purposes.

    abstract：:We performed a population study on 15 polymorphic STR loci (FH2010, FH2079, PEZ2, VWF.X, FH2054, FH2087Ub, FH2611, WILMS-TF, PEZ12, PEZ15, PEZ6, FH2087Ua, ZUBECA4, ZUBECA6, FH2132) on 131 randomly selected dogs. Alleles were identified and grouped according to their estimated fragment length using fixed allelic bins e...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-004-0452-5

    authors： Eichmann C,Berger B,Parson W

    更新日期：2004-10-01 00:00:00

  • Development of a skull/brain model for military wound ballistics studies.

    abstract：:Reports on penetrating ballistic head injuries in the literature are dominated by case studies of suicides; the penetrating ammunition usually being .22 rimfire or shotgun. The dominating cause of injuries in modern warfare is fragmentation and hence, this is the primary threat that military helmets protect the brain ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1073-2

    authors： Carr D,Lindstrom AC,Jareborg A,Champion S,Waddell N,Miller D,Teagle M,Horsfall I,Kieser J

    更新日期：2015-05-01 00:00:00

  • Characterising the dynamics of expirated bloodstain pattern formation using high-speed digital video imaging.

    abstract：:During forensic investigations, it is often important to be able to distinguish between impact spatter patterns (blood from gunshots, explosives, blunt force trauma and/or machinery accidents) and bloodstain patterns generated by expiration (blood from the mouth, nose or lungs). These patterns can be difficult to dist...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-010-0498-5

    authors： Donaldson AE,Walker NK,Lamont IL,Cordiner SJ,Taylor MC

    更新日期：2011-11-01 00:00:00

  • Population genetic analysis of the Globalfiler STR loci in 3032 individuals from the Altay Han population of Xinjiang in northwest China.

    abstract：:The genetic polymorphisms of 21 autosomal short tandem repeat (STR) loci included in the GlobalFiler™ PCR Amplification Kit were evaluated in 3032 unrelated individuals Altay Han of Xinjiang, northwest China. All of the loci reached the Hardy-Weinberg equilibrium. These loci were examined to determine allele frequenci...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-017-1641-3

    authors： Li X,Li L,Wang Q,Zhang J,Ge W,Bai R,Yu X,Shi M

    更新日期：2018-01-01 00:00:00

  • The use of post-mortem computed tomography in the investigation of intentional neonatal upper airway obstruction: an illustrated case.

    abstract：:We present a single case report illustrating the diagnostic role of multi-slice computed tomography (MSCT) in the investigation of suspicious child death where mechanical asphyxia is suspected. The case illustrates how radiological findings that may not be observable on conventional plain X-ray were identified by post...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-010-0438-4

    authors： Rutty GN,Jeffery AJ,Raj V,Morgan B

    更新日期：2010-11-01 00:00:00