Abstract:
:The 5 tetranucleotide short tandem repeats, HUMTH01, HUMVWA31/A, HUMF13A1, HUMFES/FPS and HUMLPL were studied using different electrophoretic methods and PCR amplification conditions in order to optimize the typing conditions. A genetic population study in the population of Galicia was carried out and the allele and genotype frequencies are given. Compliance with the Hardy-Weinberg equilibrium was tested using different statistical parameters, with clear advantages resulting in favor of using the exact test (Guo-Thompson method) instead of conventional chi-square methods. Some statistical parameters of forensic interest (PD, CE, h) were also calculated. There were no mutations found in a total of 73 paternal meioses and 101 maternal meioses. Abnormal electrophoretic mobility was found in the AT-rich STR HUMF13A1 under non-denaturing conditions and, therefore, the use of denaturing conditions is absolutely necessary. No "stutter" bands were found, although double peaks in the HUMFES/FPS system were observed in some samples. The advantage of using automated sequencers with fluorescent technology is also reported.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Pestoni C,Lareu MV,Rodríguez MS,Muñoz I,Barros F,Carracedo Adoi
10.1007/BF01246874subject
Has Abstractpub_date
1995-01-01 00:00:00pages
283-90issue
6eissn
0937-9827issn
1437-1596journal_volume
107pub_type
杂志文章abstract::Determinations of chronologic age are of great importance in forensic science. At present the aspartic acid racemization method on teeth provides one of the best means in adult individuals. However, if teeth are not available, some other stable tissue has to be used. In this study, the applicability of cartilage from ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF01845611
更新日期:1995-01-01 00:00:00
abstract::Forensic identification comprises legal, social, ethical, and religious aspects where age detection is an important factor. When the case is a fetus or infant, recording various measurements of the body, head, and teeth is essential. The aim of this research is to evaluate the effects of different tooth and body measu...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-013-0935-3
更新日期:2014-05-01 00:00:00
abstract::The development rate of immature Calliphora vicina reared on decomposed liver was significantly slower, by as much as 30 h (55.4 % of total development time) for mid-sized larvae, and 71 h (35.0 %) and 58 h (14.6 %) if using times to the onset of pupariation and eclosion, respectively, than those of immatures that dev...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-012-0697-3
更新日期:2013-01-01 00:00:00
abstract::Medico-legal experts are increasingly enlisted to assess the methamphetamine and amphetamine serum concentrations after a criminal offense. However, since criminal users rarely provide useful information to medico-legal experts regarding the substances abused, when the substance(s) was/were used, dose of ingestion too...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-017-1607-5
更新日期:2017-09-01 00:00:00
abstract::The use of DNA to determine the ancestry of an individual is becoming more and more important in the areas of forensics. Kidd et al. (Forensic Sci Int Genet 12:215-224, 2014) have been the first to identify and catalog haplotypes, termed as minihaplotypes (1-10-kilobase spans) and microhaplotypes (≤ 200 bp), with pote...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-018-1881-x
更新日期:2019-07-01 00:00:00
abstract::Cyanide (CN) blood concentration is hardly considered during routine when evaluating smoke gas intoxications and fire victims, although some inflammable materials release a considerable amount of hydrogen cyanide. CN can be significant for the capacity to act and can in the end even be the cause of death. Systematic d...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-016-1426-0
更新日期:2017-01-01 00:00:00
abstract::The analysis of a mixed biological stain by means of highly polymorphic VNTR systems usually reveals a profile composed of multiple alleles. If the victim and one or several suspects match the profile the evidential strength of the matches has to be very carefully analysed. The appropriate methods for the statistical ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050019
更新日期:1999-01-01 00:00:00
abstract::In the present study 161 Japanese father/son haplotype transfers in 147 pedigrees were analyzed at 14 Y-STRs with two multiplex PCR-based typing systems. Five isolated single repeat mutations were identified at the DYS389I, DYS439, Y-GATA-H4, DYS389II and DYS391 loci, and a pedigree showing triple alleles at the DYS38...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-003-0422-3
更新日期:2004-06-01 00:00:00
abstract::Allele frequencies and forensic parameters for 17 autosomal short tandem repeat (STR) loci were investigated from 1542 individuals of Va ethnic minority in Yunnan Province in the southwest of China. Also, genetic distances between Va and 13 published populations were compared, and a neighbor-joining (NJ) phylogenetic ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-017-1620-8
更新日期:2017-09-01 00:00:00
abstract::Paradoxical undressing is a phenomenon characterizing some fatal hypothermia cases. The victims, despite low environmental temperatures, paradoxically remove their clothes due to a sudden feeling of warmth. In this report, we describe a case of suspected paradoxical undressing in a non-hypothermia case. The victim, a ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-017-1597-3
更新日期:2017-09-01 00:00:00
abstract::The use of phenethylamines in the dance scene is now well established. Apart from amphetamine, the commonest phenethylamine encountered in clinical and forensic settings is 3,4-methylenedioxymethamphetamine (MDMA) commonly known as ecstasy. Other phenethylamines, which have similar effects are encountered, such as 3,4...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050291
更新日期:2000-01-01 00:00:00
abstract::A case of rupture of the superior mesenteric vein with hemoretroperitoneum is described. The autopsy revealed the presence of an acute pancreatitis and thrombosis of the superior mesenteric artery. From the histological findings it appeared that the acute pancreatitis had damaged the venous wall from the outside and t...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF01369553
更新日期:1992-01-01 00:00:00
abstract::Sequence variation of the hypervariable segments (HVS) I/II of mitochondrial DNA (mtDNA) and the haplogroup affiliation were determined in a sample of 271 Italian subjects. This analysis showed that 42% of the individuals could be ascribed to H, the most frequent haplogroup in European Caucasian populations. This frac...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-005-0059-5
更新日期:2006-05-01 00:00:00
abstract::Six synthetic head models wearing ballistic protective helmets were used to recreate two military combat-related shooting incidents (three per incident, designated 'Incident 1' and 'Incident 2'). Data on the events including engagement distances, weapon and ammunition types was collated by the Defence Science and Tech...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-018-1802-z
更新日期:2019-01-01 00:00:00
abstract::Chumbinho is the popular name given to carbamate aldicarb (Temik), an insecticide commonly used in agriculture and highly toxic (LD50 = 0.9 mg/kg oral in rats) that has been sold clandestinely in several regions of Brazil. Chumbinho is sold illegally as raticide and is available in a formulation of small black granule...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-016-1336-1
更新日期:2016-07-01 00:00:00
abstract::The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise. In this study, we sequenced the mitochondrial cytochrome c oxidase subunit I (COI) gene of 126 specimens of 56 W European Sarcophaga species and added GenBank data to our database to ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-012-0767-6
更新日期:2013-03-01 00:00:00
abstract::We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150-182 bp) than the primers in standard use (295-327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF02441025
更新日期:1997-01-01 00:00:00
abstract::A 28-year-old man was shot in the back of the head at close range by a robber who then locked him in a room assuming that he was dead. The man was discovered 2 days later. The entrance wound of the bullet was in the left occipital region and it passed into the periphery of the right temporal lobe, where it lodged. The...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004149900120
更新日期:2000-01-01 00:00:00
abstract::The population origin of an individual is often requested to be determined from specimens left at a crime scene for identifying a suspect and individual identity. The melanocortin 1 receptor gene (MC1R) and P gene are associated with human pigmentation. Although several studies have reported that these genes are highl...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-008-0289-4
更新日期:2009-05-01 00:00:00
abstract::We present a single case report illustrating the diagnostic role of multi-slice computed tomography (MSCT) in the investigation of suspicious child death where mechanical asphyxia is suspected. The case illustrates how radiological findings that may not be observable on conventional plain X-ray were identified by post...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-010-0438-4
更新日期:2010-11-01 00:00:00
abstract::Most standard procedures for extracting DNA from forensic substrates involve manipulations that expose the sample to potential contamination and which reduce yields. Furthermore, most methods require centrifugation and/or solvent extraction steps that render them difficult to automate. We describe a simple closed-tube...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-003-0400-9
更新日期:2003-12-01 00:00:00
abstract::Mitragyna speciosa (Rubiaceae), commonly known as kratom, is a tropical tree with a long history of traditional use in parts of Africa and Southeast Asia. In recent years, kratom has gained popularity for use as a recreational drug across the globe. Relatively new to the illicit market and used in a manner different f...
journal_title:International journal of legal medicine
pub_type: 杂志文章,评审
doi:10.1007/s00414-015-1279-y
更新日期:2016-01-01 00:00:00
abstract::The purpose of the present research is to study the radiographic visibility of the periodontal ligament in third molars from a northern Chinese population to determine if this methodology could be used for the 18-year threshold. A total of 1300 conventional orthopantomograms from 650 males and 650 females of northern ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-017-1664-9
更新日期:2018-03-01 00:00:00
abstract::A study was performed on the detection, separation and quantification of isomers from the new designer drugs named fluoroamphetamines (FAs) in forensic cases in eastern Denmark. The drugs were detected in whole blood extracts by ultraperformance liquid chromatography with time of flight mass spectrometer (UPLC-TOF-MS)...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-012-0671-0
更新日期:2012-07-01 00:00:00
abstract::Various aspects concerning the practical application and forensic interpretation of data obtained by saliva drug testing and drug monitoring from the skin surface are discussed. Basic information on the composition of saliva and skin secretions and their particular transport mechanisms, as far as known, are given. For...
journal_title:International journal of legal medicine
pub_type: 杂志文章,评审
doi:10.1007/s004140050239
更新日期:1999-01-01 00:00:00
abstract:PURPOSE:The response to traumatic brain injury (TBI) is complex and induces various biological pathways in all brain regions that contribute to bad outcomes. The cerebellar hypoxia after a frontal cortex injury may potentiate the pathophysiological impacts of TBI. Therefore, a gene expression analysis was conducted to ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-014-1129-3
更新日期:2015-07-01 00:00:00
abstract::Short tandem repeats (STRs) are DNA regions with a variable number of repeated units (2-6 bp) and attractive to genetic application like forensic, population genetics, and anthropological studies. The Huaxia Platinum System allows the multiplex amplification of 23 autosomal STR loci and two gender determination loci. ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-017-1679-2
更新日期:2018-07-01 00:00:00
abstract::Y-chromosomal short tandem repeats (Y-STRs) are commonly used to study population histories, discover ancestral relationships, and identify males for criminal justice purposes. Y-STRs being largely in forensic use have low haplotype diversity in some populations and cannot discriminate between paternal male relatives....
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-018-01997-9
更新日期:2019-05-01 00:00:00
abstract::Messenger RNA (mRNA) markers have been extensively investigated for the identification of forensically relevant body fluids and tissues based on their expression profiles among cell types. As products of the backsplicing of pre-mRNAs, circular RNAs (circRNAs) share exonic sequences with their linear counterparts. The ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-019-02027-y
更新日期:2019-09-01 00:00:00
abstract::The ability to estimate stature from various parts of the body is essential in medicolegal investigations, particularly in cases in which human remains have been dismembered or mutilated. Therefore, the aim of this study was to generate linear regression models for predicting stature on the basis of sternum length in ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-014-0975-3
更新日期:2014-09-01 00:00:00