Abstract：
:Messenger RNA (mRNA) markers have been extensively investigated for the identification of forensically relevant body fluids and tissues based on their expression profiles among cell types. As products of the backsplicing of pre-mRNAs, circular RNAs (circRNAs) share exonic sequences with their linear counterparts. The inclusion of circRNAs in mRNA profiling is shown to facilitate the detection of biomarkers in the identification of body fluids. In this study, we identified the expression of circRNAs of 14 out of 45 biomarkers from five body fluid types using outward-facing primer sets and revealed the ratio of circular to total transcripts of biomarkers by RNase R treatment. Furthermore, our results of qPCR analysis show that the inclusion of circRNAs in the detection of biomarkers, including HBA and ALAS2 for blood; MMP7 and MMP10 for menstrual blood; HTN3 for saliva; SPINK5, SERPINB3, ESR1, and CYP2B7P1 for vaginal secretions; TGM4, KLK3, and PRM2 for semen; and SLC22A6 and MIOX for urine, does not impair the specificity of these biomarkers. Additionally, a high copy number of targets from linear transcripts could be employed to increase the detection sensitivity of TGM4 and KLK3 with a low expression level of circRNAs in urine samples. Altogether, these results will help with the development of robust multiplex assays for body fluid identification.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Liu B,Song F,Yang Q,Zhou Y,Shao C,Shen Y,Zhao Z,Tang Q,Hou Y,Xie Jdoi
10.1007/s00414-019-02027-ysubject
Has Abstractpub_date
2019-09-01 00:00:00pages
1321-1331issue
5eissn
0937-9827issn
1437-1596pii
10.1007/s00414-019-02027-yjournal_volume
133pub_type
杂志文章abstract：:Historically, radiographical identification has been done by comparing conventional antemortem and postmortem X-ray images. The advent of new technologies such as multislice computed tomography (MSCT) is making traditional antemortem examination increasingly less frequent. The authors present the results of MSCT study...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-007-0200-8
更新日期：2007-11-01 00:00:00
abstract：:The purpose of this study is to evaluate allelic association and linkage of 18 adjacent syntenic short tandem repeat (STR) pairs form out of 30 markers located on 12 different autosomes. Linkage disequilibrium was tested by using the unknown gametic phase genotypes and phased haplotypes from 290 unrelated individuals ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1032-y
更新日期：2014-09-01 00:00:00
abstract：:This paper considers the statistical evaluation of DNA mixtures in the following situations: (1) two unknown contributors are related respectively to two typed persons, (2) two of the unknown or untyped contributors are related and the third unknown contributor is related to a typed person, or (3) there are two pairs ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-004-0493-9
更新日期：2005-09-01 00:00:00
abstract：:For a long time, it has been thought that fresh and frozen tissues are the only possible source of biological material useful to extract nucleic acids suitable for downstream molecular analysis. Recently, for forensic purpose such as personal identification, also fixed tissues have been used to recover DNA molecules, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1066-1
更新日期：2015-01-01 00:00:00
abstract：:In many forensic cases, the existence of a traumatic brain injury (TBI) is an essential factor, and the determination of the survival time is nearly as important as the determination of whether or not a trauma exists. Since it is known that glucose uptake increases in injured brain cells in order to perpetuate the neu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1893-6
更新日期：2019-07-01 00:00:00
abstract：:Determining the time of injury is an important but still a challenging task in forensic anthropology. In literature, many descriptions can be found to make a distinction between perimortem and postmortem fractures. Characteristics that are more related to fractures in fresh conditions, however, are not extensively inv...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1514-1
更新日期：2017-05-01 00:00:00
abstract：:We report about the case of a sudden unexpected death of a 25-year-old male suffering from infectious disease. An autopsy was ordered with no final premortem diagnosis. Microscopic and microbiological examination revealed a pneumococcal bronchopneumonia and hemophagocytic lesions in the bone marrow. After integrating ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1305-0
更新日期：2016-05-01 00:00:00
abstract：:Scars of human skin can on occasions provide a very useful ancillary method of identification of an unknown deceased. If the age of any visible scars can be estimated objectively, then this might be of some assistance in the identification procedure. Melanocytes migrate into scar tissue as it ages and their number wit...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100225
更新日期：2001-10-01 00:00:00
abstract：:The genetic polymorphisms of 15 autosomal short tandem repeat (STR) loci included in the AmpFISTR Sinofiler™ kit were evaluated in 547 healthy unrelated Han individuals from Gansu, China. All of the loci reached the Hardy-Weinberg equilibrium after the Bonferroni correction (p > 0.0033). These loci were examined to de...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1132-8
更新日期：2015-07-01 00:00:00
abstract：:Population studies on six short tandem repeat loci, HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 were carried out in a sample of unrelated Japanese individuals (n = 337-545) living in Gifu Prefecture (central region of Japan). Five alleles could be identified for HUMFXIIIB, six for HUMF13A01, HUMLIPO...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01369599
更新日期：1996-01-01 00:00:00
abstract：INTRODUCTION:The determination of age of majority (the 18-year-old threshold) using the popular Demirjian tooth staging method is unreliable, so other maturity markers are required. This study examines whether the Root Pulp Visibility (RPV) of the mandibular third molar is a useful indicator of age. METHODS:One thousa...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02155-5
更新日期：2020-01-01 00:00:00
abstract：:Thyroid hormones are sometimes used for purposes for which they are not approved. Reasons for off-label use can be overweight, prevailing depressive mood, or various somatic symptoms. Information about the intake of thyroid hormones in order to lose weight can be easily obtained from inappropriate/nonmedical websites....
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0423-y
更新日期：2010-11-01 00:00:00
abstract：:Six synthetic head models wearing ballistic protective helmets were used to recreate two military combat-related shooting incidents (three per incident, designated 'Incident 1' and 'Incident 2'). Data on the events including engagement distances, weapon and ammunition types was collated by the Defence Science and Tech...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1802-z
更新日期：2019-01-01 00:00:00
abstract：:The goal of the present study is to examine cross-sectional information on the growth of the humerus based on the analysis of four measurements, namely, diaphyseal length, transversal diameter of the proximal (metaphyseal) end of the shaft, epicondylar breadth and vertical diameter of the head. This analysis was perfo...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0713-7
更新日期：2013-01-01 00:00:00
abstract：:A chimera is an organism whose cells derive from two or more distinct zygote lineages. and therefore two different blood cell populations circulate in one individual. To point out the potential pitfalls in forensic analysis, a set of triplets (a girl and two boys) who revealed blood chimerism was investigated with fou...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050263
更新日期：1999-01-01 00:00:00
abstract：:In this casuistry, two accidents from Germany and Switzerland are presented that happened during the shot of recoilless anti-tank weapons. In both cases, the injuries led to the death of two soldiers: A 22-year-old soldier in Germany was struck by the counter mass of a so-called Davis gun which had been fired by a com...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02301-4
更新日期：2020-11-01 00:00:00
abstract：:Necrophagous blow fly larvae can provide accurate estimates of the minimum postmortem interval in death investigations. During larval development, predictable morphological changes occur and measurements of weight, length, and width are compared to species-specific growth curves for reliable age estimates. However, ag...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0699-1
更新日期：2013-01-01 00:00:00
abstract：:The use of tracker dogs is the main method of finding hidden bodies, and in their search the dogs use typical scent patterns. "Electronic noses" can also be used to find and compare such patterns. Highly sensitive scent detectors have been successfully applied, e.g. in the examination of foodstuffs, in environmental t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-009-0338-7
更新日期：2010-01-01 00:00:00
abstract：:Sexual assault is a complex situation with medical, psychological, and legal aspects. Forensic experts play a major role in terms of forensic and gynecological medical examination and evidence collection in order to maintain the chain of custody. Victims should be examined by a specially trained medico-legal examiner ...
journal_title：International journal of legal medicine
pub_type： 杂志文章,实务指引
doi：10.1007/s00414-018-1791-y
更新日期：2018-11-01 00:00:00
abstract：:Postmortem ethanol formation is a well-known problem in forensic toxicology. The aim of this study was to interpret findings of ethanol in blood, in a large collection of forensic autopsy cases, by use of the nonoxidative ethanol metabolites, ethyl glucuronide (EtG), and ethyl sulfate (EtS). In this study, according t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1031-z
更新日期：2014-09-01 00:00:00
abstract：:We performed a population study on 15 polymorphic STR loci (FH2010, FH2079, PEZ2, VWF.X, FH2054, FH2087Ub, FH2611, WILMS-TF, PEZ12, PEZ15, PEZ6, FH2087Ua, ZUBECA4, ZUBECA6, FH2132) on 131 randomly selected dogs. Alleles were identified and grouped according to their estimated fragment length using fixed allelic bins e...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-004-0452-5
更新日期：2004-10-01 00:00:00
abstract：:Blood, saliva, and semen are some of the forensically most relevant biological stains commonly found at crime scenes, which can often be of small size or challenging due to advanced decay. In this context, it is of great importance to possess reliable knowledge about the effects of degradation under different environm...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1373-9
更新日期：2016-11-01 00:00:00
abstract：:The original version of this article unfortunately contained a mistake in the Results section. The correct equation from the cubic regression analysis for male is: Age = 14.408 + 0.475X-0.064X2 + 0.005X3, and for the female is: 18.196-1.637X + 0.204X2-0.005X3. The error doesn't affect the main conclusion. ...
journal_title：International journal of legal medicine
pub_type： 杂志文章,已发布勘误
doi：10.1007/s00414-017-1751-y
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:We investigated the incidence and distribution of post-mortem gas detected with multidetector computed tomography (MDCT) to identify factors that could distinguish artifactual gas from cardiac air embolism. MATERIAL AND METHODS:MDCT data of 119 cadavers were retrospectively examined. Gas was semiquantitatively...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0542-5
更新日期：2012-01-01 00:00:00
abstract：:Evaluation of the radiographic visibility of root pulp in mandibular third molars has been suggested as an alternative method for estimation of legal age threshold in living individuals when the root apices are mature. Here, we assessed the accuracy of this method for age thresholds of 18 and 21 years. A sample of 463...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02036-x
更新日期：2019-09-01 00:00:00
abstract：:The molecular composition of the genetic substrate recovered from seven aged forensic samples has been extensively investigated. A simple enzymatic test based on DNAseI incubation of the extracts showed that the UV-fluorescent material from the forensic specimens is composed of nucleic acids, with the DNA fraction rep...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-002-0326-7
更新日期：2002-12-01 00:00:00
abstract：:A single gunshot (or multiple) does (do) not necessarily lead to immediate loss of consciousness or rapid neurological deficits, so the victim may be able to repeatedly pull the trigger before achieving the lethal effect. Despite multiple gunshot wounds can lead to the suspicious participation of other person to the d...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02085-2
更新日期：2019-09-01 00:00:00
abstract：:Cyanide (CN) blood concentration is hardly considered during routine when evaluating smoke gas intoxications and fire victims, although some inflammable materials release a considerable amount of hydrogen cyanide. CN can be significant for the capacity to act and can in the end even be the cause of death. Systematic d...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1426-0
更新日期：2017-01-01 00:00:00
abstract：:The authors report on a young boy who was bitten into his face by an unknown animal while being asleep in a tent. Given the bite marks and the location of the scene, members of the mustelidae and canidae families were the first "suspects." Deoxyribunucleic acid (DNA) recovered from the tent's wall was analyzed with re...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0674-x
更新日期：2012-05-01 00:00:00
abstract：:DNA typing of cellular debris from perforating bullets was investigated following shooting experiments. A total of 14 perforating gunshots were fired into 9 calves. PCR typing of tissue fragments was done using bovine-specific primers flanking a 247 bp segment within the bovine lactoglobulin gene. Positive amplificati...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01369787
更新日期：1996-01-01 00:00:00
