Abstract:
:The authors report on a young boy who was bitten into his face by an unknown animal while being asleep in a tent. Given the bite marks and the location of the scene, members of the mustelidae and canidae families were the first "suspects." Deoxyribunucleic acid (DNA) recovered from the tent's wall was analyzed with regard to parts of the mitochondrial 12S ribosomal ribunucleic acid (12S rRNA) and cytochrome b (cytb) genes as well as nuclear short tandem repeats (STRs). Since Sanger sequencing revealed a mixed sequence with a strong human component overlying the nonhuman contributor, an animal screening using a duplex real-time polymerase chain reaction (PCR) with an intercalating dye and melt curve analysis was employed. The results were later confirmed by cloning. The applied commercial canine STR kit verified the animal family (canidae) but did not help in discriminating the species due to cross-species amplification. In the presented case, the real-time PCR assay offered the cheapest and fastest method for animal family determination, which then allowed for an appropriate and sample-saving strategy to characterize the causative animal species.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Naue J,Lutz-Bonengel S,Pietsch K,Sänger T,Schlauderer N,Schmidt Udoi
10.1007/s00414-012-0674-xsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
483-8issue
3eissn
0937-9827issn
1437-1596journal_volume
126pub_type
杂志文章abstract::One of the most common conditions during fetal development is anencephaly, which often involves many identification difficulties in the context of physical anthropology, as it causes severe skull challenges. In this paper, we describe the alterations found in the skulls of two perinatal individuals with anencephaly fr...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-014-1123-9
更新日期:2015-07-01 00:00:00
abstract::From sequence database information, we have identified two male-specific and polymorphic tetranucleotide STRs, DYS 441 (GDB:10013873) and DYS 442 (GDB: 10030304), on the Y chromosome. Analysis of 184 males allowed 7 and 5 alleles to be distinguished in the DYS 441 and DYS 442 systems, respectively, yielding 21 haploty...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140100207
更新日期:2001-08-01 00:00:00
abstract::The analysis of a mixed biological stain by means of highly polymorphic VNTR systems usually reveals a profile composed of multiple alleles. If the victim and one or several suspects match the profile the evidential strength of the matches has to be very carefully analysed. The appropriate methods for the statistical ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050019
更新日期:1999-01-01 00:00:00
abstract::The assessment of age at death for identification purposes is a frequent and tough challenge for forensic pathologists and anthropologists. Too frequently, visual assessment of age is performed on well-preserved corpses, a method considered subjective and full of pitfalls, but whose level of inadequacy no one has yet ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-014-1042-9
更新日期:2015-07-01 00:00:00
abstract::Kinship relation and, in particular, paternity probability estimation using a Bayesian approach require the input of a priori probabilities of different hypotheses. In practical case work, a priori probabilities or priors, for short, must often be estimated using only common sense and symmetry arguments because in mos...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-013-0827-6
更新日期:2013-11-01 00:00:00
abstract::A 12-year-old boy with no previous serious medical history experienced abdominal discomfort and chest pains for 5 days and suddenly died. The autopsy revealed diffuse and extensive infiltration of eosinophils into the myocardium, with poorly formed granulomas and few fibrotic changes. The necrotic changes was so exten...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF01369797
更新日期:1996-01-01 00:00:00
abstract::The first aim of this study was to assess the diagnostic performance of presepsin (sCD14-ST) in postmortem serum from femoral blood compared to procalcitonin (PCT) to detect sepsis-related fatalities. The second aim was to compare sCD14-ST levels found in postmortem serum to the values in pericardial fluid to investig...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-012-0804-5
更新日期:2013-07-01 00:00:00
abstract::A medicolegal/algological collaboration lasting several years aimed at developing methods for dealing with dead bodies found in water where the circumstances are not clear, has led to an interdisciplinary procedure. To enable algological analysis, sample preservation and preparation must be free from contamination or ...
journal_title:International journal of legal medicine
pub_type: 杂志文章,评审
doi:10.1007/s004149900122
更新日期:2000-01-01 00:00:00
abstract::A block of an injury instrument will be left in wounds sometimes, and the suspect instrument can be discriminated by comparison with the block that was left through elemental analysis. In this study, three brands (Shibazi, Zhangxiaoquan, Qiaoxifu) of kitchen knives with forged, chop, and slice application series were ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-013-0829-4
更新日期:2014-03-01 00:00:00
abstract::The aim of this study was to measure catecholamines and their O-methylated metabolites in urine and vitreous humor collected in cardiac deaths and noncardiac control cases that underwent medicolegal investigations. Our first goal was to assess whether cardiac events of different types are characterized by different ca...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-015-1303-2
更新日期:2016-07-01 00:00:00
abstract::A publically available database of the most highly cited scientists in all disciplines was used to identify people that belonged to the subject category "forensic science and legal medicine." This bibliometric information was derived from Elsevier's SCOPUS database containing eight million scientists with at least fiv...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-020-02491-x
更新日期:2021-01-02 00:00:00
abstract::The development of online software designed for genetic studies has been exponentially growing, providing numerous benefits to the scientific community. However, they should be used with care, since some require adjustments. The efficiency of two programs for haplogroup prediction was tested with 119 samples of known ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-009-0404-1
更新日期:2011-01-01 00:00:00
abstract::Article 25 of the Charter of Fundamental Rights of the European Union (adopted in Nice on 7 December 2000) recognizes and respects the rights of older people to lead a life of dignity and independence and to participate in social and cultural life. It also highlights the importance of prevention and recognition of eld...
journal_title:International journal of legal medicine
pub_type: 杂志文章,实务指引
doi:10.1007/s00414-018-1880-y
更新日期:2019-01-01 00:00:00
abstract::Evaluation of the radiographic visibility of root pulp in mandibular third molars has been suggested as an alternative method for estimation of legal age threshold in living individuals when the root apices are mature. Here, we assessed the accuracy of this method for age thresholds of 18 and 21 years. A sample of 463...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-019-02036-x
更新日期:2019-09-01 00:00:00
abstract::The original version of this article unfortunately contained a mistake in the Results section. The correct equation from the cubic regression analysis for male is: Age = 14.408 + 0.475X-0.064X2 + 0.005X3, and for the female is: 18.196-1.637X + 0.204X2-0.005X3. The error doesn't affect the main conclusion. ...
journal_title:International journal of legal medicine
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00414-017-1751-y
更新日期:2018-01-01 00:00:00
abstract::An accurate and precise documentation of injuries is fundamental in a forensic pathological context. Photographs and manual measurements are taken of all injuries during autopsies, but ordinary photography projects a 3D wound on a 2D space. Using technologies such as photogrammetry, it is possible to create 3D detaile...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-016-1499-9
更新日期:2017-05-01 00:00:00
abstract::The tetrameric STRs, HUMTH01, HUMVWA31A and HUMFES/FPS, were studied in a population from the Basque Country (northern Spain) for their frequency distribution and applicability to identity and paternity testing. All systems conformed to Hardy-Weinberg equilibrium; pairwise comparisons demonstrated the allelic independ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF01245481
更新日期:1995-01-01 00:00:00
abstract::Diagnosis of ischaemia-related sudden cardiac death in the absence of microscopic and macroscopic ischaemic lesions remains a challenge for medical examiners. Medical imaging techniques increasingly provide support in post-mortem examinations by detecting and documenting internal findings prior to autopsy. Previous st...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-019-02211-0
更新日期:2020-03-01 00:00:00
abstract::To examine the prevalence of hepatitis G virus (HGV) and hepatitis C virus (HCV) infections in deceased injection drug users and for comparison of the detection rates of HGV and HCV RNA in liver tissue with detection rates in postmortem serum samples, RT-PCR was performed in 50 drug abuse-related fatalities. HGV RNA w...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050195
更新日期:1998-01-01 00:00:00
abstract::The purpose of the present research is to study the radiographic visibility of the periodontal ligament in third molars from a northern Chinese population to determine if this methodology could be used for the 18-year threshold. A total of 1300 conventional orthopantomograms from 650 males and 650 females of northern ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-017-1664-9
更新日期:2018-03-01 00:00:00
abstract::We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up....
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-008-0223-9
更新日期:2008-05-01 00:00:00
abstract::Blood, saliva, and semen are some of the forensically most relevant biological stains commonly found at crime scenes, which can often be of small size or challenging due to advanced decay. In this context, it is of great importance to possess reliable knowledge about the effects of degradation under different environm...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-016-1373-9
更新日期:2016-11-01 00:00:00
abstract::Allele frequencies and forensic statistics of 13 autosomal short tandem repeat loci were estimated in 56 unrelated Iranian individuals. Except for two loci which were monomorph, a total of 5-11 alleles at each locus were observed and altogether 94 alleles for all selected loci were found. Our results show that 11 out ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-016-1342-3
更新日期:2016-11-01 00:00:00
abstract::This paper reports population data and statistics for the HumFES/FPS, HumVWA, HumFGA and D12S391 loci in Austria. The sequences of some rare and new variant alleles which have been identified in the course of the present population study and other investigations are described. Sequence variation occurred in a HumFES/F...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/pl00007712
更新日期:2000-01-01 00:00:00
abstract::The measurement of the myeloperoxidase activity was used to quantify the acute granulocyte reaction. A 35% loss of blood resulted in a clear decrease of the myeloperoxidase activity at the edges of experimental incision wounds in rat skin in the first day after inflicting the wounds. After 12 h only about one-third an...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050101
更新日期:1998-01-01 00:00:00
abstract::Warping has been used to determine the pre-burning condition of human skeletal remains. In the literature, this modification has been associated more often with the burning of fleshed and green bones, but it also arises during the burning of dry bones. The objective of this paper was to assess if bone collagen content...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-016-1400-x
更新日期:2016-11-01 00:00:00
abstract::Scars of human skin can on occasions provide a very useful ancillary method of identification of an unknown deceased. If the age of any visible scars can be estimated objectively, then this might be of some assistance in the identification procedure. Melanocytes migrate into scar tissue as it ages and their number wit...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140100225
更新日期:2001-10-01 00:00:00
abstract::P-glycoprotein (P-gp), encoded by the ABCB1/MDR1 gene, is a drug transporter at the blood-brain barrier. Several polymorphisms in the ABCB1 gene are known to affect the activity and/or expression of P-gp, thereby influencing the treatment response and toxicity of P-gp substrates like citalopram and venlafaxine. In thi...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-013-0849-0
更新日期:2013-05-01 00:00:00
abstract::Cocaine and alcohol toxicity is well known, especially when simultaneously abused. These drugs perform both acute and chronic harmfulness, with significant cardiac events such as ventricular arrhythmias, tachycardia, systemic hypertension, acute myocardial infarction, ventricular hypertrophy, and acute coronary syndro...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-014-1119-5
更新日期:2015-01-01 00:00:00
abstract::Messenger RNA (mRNA) markers have been extensively investigated for the identification of forensically relevant body fluids and tissues based on their expression profiles among cell types. As products of the backsplicing of pre-mRNAs, circular RNAs (circRNAs) share exonic sequences with their linear counterparts. The ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-019-02027-y
更新日期:2019-09-01 00:00:00