Abstract:
:Blood, saliva, and semen are some of the forensically most relevant biological stains commonly found at crime scenes, which can often be of small size or challenging due to advanced decay. In this context, it is of great importance to possess reliable knowledge about the effects of degradation under different environmental conditions and to use appropriate methods for retrieving maximal information from limited sample amount. In the last decade, RNA analysis has been demonstrated to be a reliable approach identifying the cell or tissue type of an evidentiary body fluid trace. Hence, messenger RNA (mRNA) profiling is going to be implemented into forensic casework to supplement the routinely performed short tandem repeat (STR) analysis, and therefore, the ability to co-isolate RNA and DNA from the same sample is a prerequisite. The objective of this work was to monitor and compare the degradation process of both nucleic acids for human blood, saliva, and semen stains at three different concentrations, exposed to dry and humid conditions during a 17-month time period. This study also addressed the question whether there are relevant differences in the efficiency of automated, magnetic bead-based single DNA or RNA extraction methods compared to a manually performed co-extraction method using silica columns. Our data show that mRNA, especially from blood and semen, can be recovered over the entire time period surveyed without compromising the success of DNA profiling; mRNA analysis indicates to be a robust and reliable technique to identify the biological source of aged stain material. The co-extraction method appears to provide mRNA and DNA of sufficient quantity and quality for all different forensic investigation procedures. Humidity and accompanied mold formation are detrimental to both nucleic acids.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Sirker M,Schneider PM,Gomes Idoi
10.1007/s00414-016-1373-9subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1431-1438issue
6eissn
0937-9827issn
1437-1596pii
10.1007/s00414-016-1373-9journal_volume
130pub_type
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journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-015-1297-9
更新日期:2016-03-01 00:00:00
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journal_title:International journal of legal medicine
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doi:10.1007/s00414-020-02333-w
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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doi:10.1007/BF01369553
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-013-0817-8
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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pub_type: 杂志文章
doi:10.1007/s00414-021-02501-6
更新日期:2021-01-27 00:00:00
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
abstract::Population studies on six short tandem repeat loci, HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 were carried out in a sample of unrelated Japanese individuals (n = 337-545) living in Gifu Prefecture (central region of Japan). Five alleles could be identified for HUMFXIIIB, six for HUMF13A01, HUMLIPO...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF01369599
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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