Abstract:
:In this study, the effect of sample purification on total signal intensities of samples amplified with genRES MPX-2 (nine-locus multiplex) prior to capillary electrophoretic analysis has been investigated. Sample purification with the Qiaquick PCR purification kit led to an increase of the relative fluorescent signal intensity by a factor of 3.8 +/- 0.8. In contrast, the application of larger sample volumes led to a decrease of signal intensities from 20% to 80%, depending on whether the samples were purified or not. In addition, increase of injection time showed a linear increase of signal intensity between 3 s and 10 s. Increasing the number of PCR cycles from 30 to 33 also led to a significant increase of signal intensities. Nevertheless, this increase greatly depended on the fragment lengths and was sometimes accompanied by the appearance of non-specific signals. In combination, optimisation of sample preparation and increase of injection time may intensify signals up to 12-fold, thereby increasing the overall sensitivity of the assay. This may be of special interest for forensic analysis of microspecimens containing limited amounts of DNA.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Lederer T,Braunschweiger G,Betz P,Seidl Sdoi
10.1007/s00414-001-0277-4subject
Has Abstractpub_date
2002-06-01 00:00:00pages
165-9issue
3eissn
0937-9827issn
1437-1596journal_volume
116pub_type
杂志文章abstract::Diffeomorphic shape registration allows for the seamless geometric alignment of shapes. In this study, we demonstrated the use of a registration algorithm to automatically seed anthropological landmarks on the CT images of the pelvis. We found a high correlation between manually and automatically seeded landmarks. The...
journal_title:International journal of legal medicine
pub_type: 杂志文章
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abstract::Bullet velocity is a basic parameter in wound ballistics studies. It is usually measured electronically by means of a variety of solid or photoelectric barriers connected to equipment measuring the time elapsing between impulses, enabling the velocity to be calculated. With the advent of Doppler radar velocity measure...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/BF01247266
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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doi:10.1007/s004140100239
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journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-008-0237-3
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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abstract::Gabapentin (GBP) (Neurontin®, Horizant®, Gralise®) is a widely prescribed medication used primarily for the treatment of epilepsy and neuropathic pain. GBP has a favorable adverse effect profile in therapeutic dosing with the most common reported effects being dizziness, fatigue, drowsiness, weight gain, and periphera...
journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050124
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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abstract::We measured the quantity of D-aspartic acid (degree of racemization of aspartic acid) in the enamel of different types of teeth from the same individual. We studied the correlation between the degree of racemization and the time of formation of each particular tooth, as well as the applicability of the degree of racem...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s00414-004-0506-8
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abstract::Forensic age estimation of living individuals has become increasingly important in forensic practice with the constant increase of migration movements to developed countries. The method of Greulich and Pyle is one of the most frequently used methods for age determination. The aim of our study was to verify the reliabi...
journal_title:International journal of legal medicine
pub_type: 杂志文章
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journal_title:International journal of legal medicine
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doi:10.1007/s00414-020-02432-8
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abstract::A total of 17 Y-specific STR loci were studied in 12 districts of the European part of Russia aiming to ascertain the amount of substructure required for the construction of a representative regional database. All groups exhibited high haplotype diversities but low inter-population variance as measured by an analysis ...
journal_title:International journal of legal medicine
pub_type: 杂志文章
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abstract::An accurate and precise documentation of injuries is fundamental in a forensic pathological context. Photographs and manual measurements are taken of all injuries during autopsies, but ordinary photography projects a 3D wound on a 2D space. Using technologies such as photogrammetry, it is possible to create 3D detaile...
journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140050205
更新日期:1998-01-01 00:00:00
abstract::STR-based individualisation of biological deposits on bullets after perforation of tissue, can identify the person injured or killed by a particular bullet and comparison with the firearms used can identify the weapon and thus possibly the person who did the shooting. In this study, the effect of subsequent impacts on...
journal_title:International journal of legal medicine
pub_type: 杂志文章
doi:10.1007/s004140000203
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abstract::Hereditary hemochromatosis (HH) is a frequent autosomal recessive disease which causes iron-overload of various organs. Of all northern European affected individuals, 90-95% show 1 of 3 known point mutations in the HFE gene. Symptoms and organs involved can vary considerably: Only a small fraction of the 200,000-400,0...
journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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abstract::We have analyzed 11 Y-STR loci (DYS19, the two DYS385 loci, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DXYS156Y) in 700 males from ten ethnic groups in east Asia in order to evaluate their usefulness for forensic and population genetic studies. A total of 644 different haplotypes were identified, among which ...
journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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