Abstract：
:During the testing of alternative primers for the Y chromosome short tandem repeat marker DYS19, a duplicated region of the Y chromosome was discovered. The duplicated sequence is contained within GenBank accession AC006335 and has a high degree of homology with the DYS19 flanking region (GenBank accession AC017019) but without the polymorphic TAGA repeat. Bioinformatic approaches have been taken to try and understand the implications of this homolog to enable improved primer design for DYS19. Sequence alignments and careful placement of primers in order to obtain specific amplification of the DYS19 locus are discussed in the context of all previously published primer sets. Since the DYS19 locus is part of the widely used minimal haplotype, its robust amplification is highly desirable particularly in multiplex reactions. The discovery of this duplicated region of the Y chromosome shows the value of newly available human genome sequence information for assay design and the importance of using sequence queries and alignments in the primer design process.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Butler JM,Schoske Rdoi
10.1007/s00414-004-0436-5subject
Has Abstractpub_date
2004-06-01 00:00:00pages
178-83issue
3eissn
0937-9827issn
1437-1596journal_volume
118pub_type
杂志文章abstract：:The main criteria used in dental age estimation in living young individuals are mineralisation and eruption of the third molars. In order to further diversify the spectrum of characteristics after completion of third molar development, tests were undertaken to determine whether the characteristics studied by Gustafson...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0701-y
更新日期：2012-07-01 00:00:00
abstract：:The allele frequencies of DYS19 and DYS390 located on the Y chromosomes were determined from male bloodstain samples of 308 (DYS19) and 268 (DYS390) unrelated male adults in south-east Hungary. Six alleles could be distinguished in both systems, yielding 22 DYS19/DYS390 halotypes in a subset of 239 males. ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050236
更新日期：1999-01-01 00:00:00
abstract：:Allele frequencies and forensic parameters for 17 autosomal short tandem repeat (STR) loci were investigated from 1542 individuals of Va ethnic minority in Yunnan Province in the southwest of China. Also, genetic distances between Va and 13 published populations were compared, and a neighbor-joining (NJ) phylogenetic ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1620-8
更新日期：2017-09-01 00:00:00
abstract：:We report a simultaneous study of the VWA STR locus by the Perkin-Elmer Profiler Plus kit and the Promega GenePrint CTTv kit in a population sample from North Portugal and in 418 meiosis from family material and paternity cases. PCR amplification and genotyping were performed according to the manufacturer's instructio...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100215
更新日期：2001-10-01 00:00:00
abstract：:Forensic statistical parameters based on allelic frequencies of commonly used short tandem repeats were estimated for the Han population of Jiangsu province from P.R. China. The 6-dye GlobalFiler™ PCR amplification kit incorporates 21 autosomal STRs, providing reliable DNA typing results with enhanced the power of dis...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1815-7
更新日期：2018-09-01 00:00:00
abstract：:Short tandem repeats (STRs) are DNA regions with a variable number of repeated units (2-6 bp) and attractive to genetic application like forensic, population genetics, and anthropological studies. The Huaxia Platinum System allows the multiplex amplification of 23 autosomal STR loci and two gender determination loci. ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1679-2
更新日期：2018-07-01 00:00:00
abstract：:Recent studies show that ethyl glucuronide (EtG) can be decomposed by bacteria; whilst so far no degradation of ethyl sulphate (EtS) has been observed. In the present study, in vitro experiments with bacterial colonies were performed. Bacteria (Escherichia coli, Klebsiella pneumoniae, Clostridium sordellii) were isola...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0229-3
更新日期：2008-09-01 00:00:00
abstract：:Expert witness opinion based on the comparison of images has been accepted by UK courts as admissible evidence in relation to issues of identity. Within images of the hand are a multiplicity of anatomical features of different aetiology, incidence and distribution patterns and this includes melanocytic nevi, referred ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0821-z
更新日期：2014-05-01 00:00:00
abstract：:A short tandem repeat at the D1S1656 locus was sequenced in 45 selected alleles and 13 different alleles were found which were designated according to the total number of repeats. This STR is a compound hypervariable STR consisting of blocks of (TAGR) repeats with a basic sequence structure (TAGA)4(TGA)0-1(TAGA)6-16(T...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050161
更新日期：1998-01-01 00:00:00
abstract：:According to European regulations and the legislations of individual member states, children who seek asylum have a different set of rights than adults in a similar position. To protect these rights and ensure rule of law, migration authorities are commonly required to assess the age of asylum seekers who lack reliabl...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1730-3
更新日期：2018-05-01 00:00:00
abstract：:The diagnosis of drowning is one of the most difficult in forensic medicine. Forensic diatomology has been proposed to be useful in solving the diagnosis of drowning and considered to be a reliable indicator of the site of drowning. The Yangtze River and Jialing River are the main rivers in the Chongqing area (China),...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02297-x
更新日期：2020-07-01 00:00:00
abstract：:In humans, the amelogenin gene is present on both the X and the Y chromosomes. However, there are size differences in this gene between these chromosomes, which have been utilised for sexing in forensic casework and prenatal diagnosis. Our study using the AmpFl STR Profiler Plus kit, showed a deletion of Y chromosome-...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-001-0262-y
更新日期：2002-04-01 00:00:00
abstract：:In order to increase the validity of age estimation in adolescents and young adults when there is no legitimation for X-ray examinations, it seems desirable to be able to assess the mineralization of third molars using X-ray-free imaging procedures. In the present study, the mineralization stages of lower third molars...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1238-7
更新日期：2015-11-01 00:00:00
abstract：:Most standard procedures for extracting DNA from forensic substrates involve manipulations that expose the sample to potential contamination and which reduce yields. Furthermore, most methods require centrifugation and/or solvent extraction steps that render them difficult to automate. We describe a simple closed-tube...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-003-0400-9
更新日期：2003-12-01 00:00:00
abstract：:We analysed 15 autosomal STRs in 200 unrelated individuals (102 males and 98 females) and 17 Y-STRs in 102 unrelated males living in Jharkhand, India, to establish parameters of forensic interest. The examined autosomal STRs revealed high combined power of exclusion (CPE) and combined power of discrimination (CPD) as ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1610-x
更新日期：2018-01-01 00:00:00
abstract：:This paper describes the general design and main results of the Italian proficiency testing program for the analysis of psychoactive substances in urine, a long-term initiative created in 1995 on an educational basis and characterized by an innovative internet-based service for data exchange between laboratories and t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050279
更新日期：1999-01-01 00:00:00
abstract：:Determining the time of injury is an important but still a challenging task in forensic anthropology. In literature, many descriptions can be found to make a distinction between perimortem and postmortem fractures. Characteristics that are more related to fractures in fresh conditions, however, are not extensively inv...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1514-1
更新日期：2017-05-01 00:00:00
abstract：:Immunohistochemistry is a well-established technique used in many research laboratories as well as in clinical diagnostics. The method allows to visualize the expression of proteins in biological tissues, as well as to evaluate this expression semi-quantitatively. For diagnosis, an optimal staining, based on a straigh...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1575-9
更新日期：2017-11-01 00:00:00
abstract：:Reports on penetrating ballistic head injuries in the literature are dominated by case studies of suicides; the penetrating ammunition usually being .22 rimfire or shotgun. The dominating cause of injuries in modern warfare is fragmentation and hence, this is the primary threat that military helmets protect the brain ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1073-2
更新日期：2015-05-01 00:00:00
abstract：:The 5 tetranucleotide short tandem repeats, HUMTH01, HUMVWA31/A, HUMF13A1, HUMFES/FPS and HUMLPL were studied using different electrophoretic methods and PCR amplification conditions in order to optimize the typing conditions. A genetic population study in the population of Galicia was carried out and the allele and g...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01246874
更新日期：1995-01-01 00:00:00
abstract：:In clinical and forensic practice, fatal thromboembolism is a major problem, particularly in a patient with no pre-existing risk factors. Out of a recent case, we discuss here the criteria for age determination of venous thrombi in a 46-year-old female with concomitant deep vein thrombosis in the common femoral left v...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1705-4
更新日期：2018-05-01 00:00:00
abstract：:Immunohistochemical investigation of the respiratory tract and lungs of 63 fire victims revealed a statistically significant enhanced expression of heat-shock protein 70 (Hsp70) in the epiglottis, the trachea, and the main and the peripheral bronchi compared with a control group. In the fire victims, a strong expressi...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0105-y
更新日期：2006-11-01 00:00:00
abstract：:Blank cartridge guns are generally regarded as being harmless and are not considered to be firearms in most countries. A comparison of the legal situations in Germany and Austria concerning weapons is given. There have been several reports of serious injuries and even fatalities due to these weapons. Ballistic experim...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050156
更新日期：1998-01-01 00:00:00
abstract：:Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which recognize the pericentromeric regions DXZ1 and DYZ1/DYZ3 of the X...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225497
更新日期：1994-01-01 00:00:00
abstract：:To investigate the genetic variation and forensic efficiency of 19 X-STR loci in the Zhuang and Mulao minorities, we calculated allele frequencies, forensic parameters, and haplotype frequencies of 405 Zhuang and 234 Mulao unrelated healthy individuals from Guangxi Zhuang Autonomous Region of China. Furthermore, we an...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02004-5
更新日期：2020-03-01 00:00:00
abstract：:A nucleotide polymorphism of C or T was detected at position 465 in the sex-determining region Y (SRY) gene. To evaluate the utility of this dimorphism in human population studies, the frequency and the frequency of the haplotype combined with the two polymorphic loci YAP and M9 were examined in a total of 130 unrelat...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004149900111
更新日期：2001-01-01 00:00:00
abstract：:In many forensic cases, the existence of a traumatic brain injury (TBI) is an essential factor, and the determination of the survival time is nearly as important as the determination of whether or not a trauma exists. Since it is known that glucose uptake increases in injured brain cells in order to perpetuate the neu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1893-6
更新日期：2019-07-01 00:00:00
abstract：:The brains of 70 fatal cases with AIDS were studied by means of immunohistochemistry and in-situ hybridization in a consecutive autopsy series (1985-July 1992). In addition, the neuropathological changes were correlated with the neurological and neuroimaging findings. Opportunistic infections included toxoplasmosis (1...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01222119
更新日期：1993-01-01 00:00:00
abstract：:Postmortem ethanol formation is a well-known problem in forensic toxicology. The aim of this study was to interpret findings of ethanol in blood, in a large collection of forensic autopsy cases, by use of the nonoxidative ethanol metabolites, ethyl glucuronide (EtG), and ethyl sulfate (EtS). In this study, according t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1031-z
更新日期：2014-09-01 00:00:00
abstract：:DNA typing was carried out on epithelial cells which were transferred from the hands of the suspect onto the neck of the victim. In an experimental study 16 suspect-victim combinations were investigated for estimating the typing success. Alternatively to an attack against the neck, the upper arm was used for "strangul...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050063
更新日期：1997-01-01 00:00:00
