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An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase.

Abstract：

:Most standard procedures for extracting DNA from forensic substrates involve manipulations that expose the sample to potential contamination and which reduce yields. Furthermore, most methods require centrifugation and/or solvent extraction steps that render them difficult to automate. We describe a simple closed-tube DNA extraction procedure using a proteinase from the thermophilic Bacillus species EA1 that produces good DNA yields from a wide range of forensic substrates. The reaction is controlled by a temperature shift regime programmed into a thermal cycler and so eliminates the need for solvent extraction or column purification. The new method is ideally suited to forensic samples where exposure to extraneous contaminating DNA must be avoided. In addition, The simplicity of the procedure makes it suitable for automation.

journal_name

Int J Legal Med

journal_title

International journal of legal medicine

authors

Moss D,Harbison SA,Saul DJ

doi

10.1007/s00414-003-0400-9

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

340-9

issue

6

eissn

0937-9827

issn

1437-1596

journal_volume

117

pub_type

杂志文章

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