Abstract：
:Linking biological samples found at a crime scene with the actual crime event represents the most important aspect of forensic investigation, together with the identification of the sample donor. While DNA profiling is well established for donor identification, no reliable methods exist for timing forensic samples. Here, we provide for the first time a biochemical approach for determining deposition time of human traces. Using commercial enzyme-linked immunosorbent assays we showed that the characteristic 24-h profiles of two circadian hormones, melatonin (concentration peak at late night) and cortisol (peak in the morning) can be reproduced from small samples of whole blood and saliva. We further demonstrated by analyzing small stains dried and stored up to 4 weeks the in vitro stability of melatonin, whereas for cortisol a statistically significant decay with storage time was observed, although the hormone was still reliably detectable in 4-week-old samples. Finally, we showed that the total protein concentration, also assessed using a commercial assay, can be used for normalization of hormone signals in blood, but less so in saliva. Our data thus demonstrate that estimating normalized concentrations of melatonin and cortisol represents a prospective approach for determining deposition time of biological trace samples, at least from blood, with promising expectations for forensic applications. In the broader context, our study opens up a new field of circadian biomarkers for deposition timing of forensic traces; future studies using other circadian biomarkers may reveal if the time range offered by the two hormones studied here can be specified more exactly.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Ackermann K,Ballantyne KN,Kayser Mdoi
10.1007/s00414-010-0457-1subject
Has Abstractpub_date
2010-09-01 00:00:00pages
387-95issue
5eissn
0937-9827issn
1437-1596journal_volume
124pub_type
杂志文章abstract：:The diagnosis of drowning is one of the most difficult in forensic medicine. Forensic diatomology has been proposed to be useful in solving the diagnosis of drowning and considered to be a reliable indicator of the site of drowning. The Yangtze River and Jialing River are the main rivers in the Chongqing area (China),...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02297-x
更新日期：2020-07-01 00:00:00
abstract：:We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150-182 bp) than the primers in standard use (295-327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF02441025
更新日期：1997-01-01 00:00:00
abstract：:We report a simultaneous study of the VWA STR locus by the Perkin-Elmer Profiler Plus kit and the Promega GenePrint CTTv kit in a population sample from North Portugal and in 418 meiosis from family material and paternity cases. PCR amplification and genotyping were performed according to the manufacturer's instructio...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100215
更新日期：2001-10-01 00:00:00
abstract：:When blood reaches an extracorporeal surface, a drying process is initiated. Properties of this drying process may be crucial for the correct assessment of case-specific time lapses, however, there is a lack of systematic studies concerning the drying times of blood. We present a study on drying properties of small bl...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0734-2
更新日期：2012-09-01 00:00:00
abstract：:Dendritic cells (DCs) can essentially contribute to innate and adaptive immune system in various organs. A double-color immunofluorescence analysis was carried out with anti-CD11c and -HLA-DRα antibodies to detect DCs in 53 skin wounds (their postinfliction intervals: group I, 0-3 days; group II, 4-7 days; group III, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02185-z
更新日期：2020-03-01 00:00:00
abstract：:Due to the requirement to minimise exposure to radiation, it is desirable to develop non-ionising imaging procedures for the analysis of skeletal maturation for forensic age diagnostics in living individuals. The present pilot study analyses the applicability of ultrasound examinations for the evaluation of apophyseal...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-011-0554-9
更新日期：2011-03-01 00:00:00
abstract：:The analysis of a mixed biological stain by means of highly polymorphic VNTR systems usually reveals a profile composed of multiple alleles. If the victim and one or several suspects match the profile the evidential strength of the matches has to be very carefully analysed. The appropriate methods for the statistical ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050019
更新日期：1999-01-01 00:00:00
abstract：:A total of 34 deployed driver and passenger airbags from altogether 20 vehicles after frontal collisions were investigated. In 80% of the airbags possible biological traces could be located with an alternative light source (ALS, Polilight) at a wavelength of 450-470 nm. These traces were swabbed, a part of them additi...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-003-0406-3
更新日期：2004-02-01 00:00:00
abstract：:Fatal head trauma (FHT) represents one of the most frequent causes of death diagnosed in forensic pathology. However, profound statistic autopsy data on FHT is still sparse. Therefore, the purpose of this study was to investigate the circumstances and injury patterns of FHT with particular focus on age and sex, and ad...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02187-x
更新日期：2020-01-01 00:00:00
abstract：:Diagnosis of ischaemia-related sudden cardiac death in the absence of microscopic and macroscopic ischaemic lesions remains a challenge for medical examiners. Medical imaging techniques increasingly provide support in post-mortem examinations by detecting and documenting internal findings prior to autopsy. Previous st...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02211-0
更新日期：2020-03-01 00:00:00
abstract：:Messenger RNA (mRNA) markers have been extensively investigated for the identification of forensically relevant body fluids and tissues based on their expression profiles among cell types. As products of the backsplicing of pre-mRNAs, circular RNAs (circRNAs) share exonic sequences with their linear counterparts. The ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02027-y
更新日期：2019-09-01 00:00:00
abstract：:The unique case of a 50-year-old known alcoholic whose corpse was exhumed 27 years after death is reported. The man apparently committed suicide by hanging, but many years later the case was questioned and homicide-linked to a long-lasting serial killer case-was suspected. Thus, the corpse was exhumed, and at the auto...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0273-z
更新日期：2008-11-01 00:00:00
abstract：:Allele frequencies of 15 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA) were determined in a sample of 163 unrelated individuals from the Republic of Macedonia. AmpFISTR Identifiler Kit (Applied Biosystems) was used for PCR amplificatio...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-005-0025-2
更新日期：2006-01-01 00:00:00
abstract：:In order to establish a reliable age indicator in the period when all other epiphyseal age indicators have already been inactivated, medial clavicle as the bone with the longest period of growth became the object of various investigations. However, the lack of population-specific method often made it unreliable in som...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0791-6
更新日期：2013-05-01 00:00:00
abstract：:Current analysis of forensic DNA stains relies on the probabilistic interpretation of bulk-processed samples that represent mixed profiles consisting of an unknown number of potentially partial representations of each contributor. Single-cell methods, in contrast, offer a solution to the forensic DNA mixture problem b...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-021-02503-4
更新日期：2021-01-23 00:00:00
abstract：:Advances in forensic identification using molecular genetics are helpful in resolving some historical mysteries. The aim of this study was to confirm the authenticity of shrunken-head artifacts exhibited by two Polish museums. Shrunken heads, known as tsantsas, were headhunting trophies of South American Indians (Jiva...
journal_title：International journal of legal medicine
pub_type： 历史文章,杂志文章
doi：10.1007/s00414-016-1448-7
更新日期：2017-05-01 00:00:00
abstract：:Myocardial pathology and cardiac functional abnormalities known to occur in association with chronic alcohol abuse have been attributed to a direct toxic effect of ethanol on the intra-myocardial vasculature: alcohol-induced occlusion of small arteries with consequent secondary ischaemia leads to individual myocyte lo...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225513
更新日期：1996-01-01 00:00:00
abstract：:Volatile substances are used widespread, especially among young people, as a cheap and easily accessible drug. Tetrachloroethylene is one of the solvents exerting effects on the central nervous system with experiences of disinhibition and euphoria. The case presented is that of a 27-year-old female, found dead by her ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1143-0
更新日期：2015-05-01 00:00:00
abstract：:DXS8378, DXS7132, HPRTB, and DXS7423 microsatellite markers located in four different X-chromosomal linkage groups were studied in the Hungarian population. After genotyping unrelated men (219) and women (165), forensic efficiency parameters showing that the four X-linked short tandem repeats are informative for foren...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-005-0051-0
更新日期：2007-01-01 00:00:00
abstract：:Scars of human skin can on occasions provide a very useful ancillary method of identification of an unknown deceased. If the age of any visible scars can be estimated objectively, then this might be of some assistance in the identification procedure. Melanocytes migrate into scar tissue as it ages and their number wit...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100225
更新日期：2001-10-01 00:00:00
abstract：:In this study, the effect of sample purification on total signal intensities of samples amplified with genRES MPX-2 (nine-locus multiplex) prior to capillary electrophoretic analysis has been investigated. Sample purification with the Qiaquick PCR purification kit led to an increase of the relative fluorescent signal ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-001-0277-4
更新日期：2002-06-01 00:00:00
abstract：:The goal of the present study is to examine cross-sectional information on the growth of the humerus based on the analysis of four measurements, namely, diaphyseal length, transversal diameter of the proximal (metaphyseal) end of the shaft, epicondylar breadth and vertical diameter of the head. This analysis was perfo...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0713-7
更新日期：2013-01-01 00:00:00
abstract：:An accurate and precise documentation of injuries is fundamental in a forensic pathological context. Photographs and manual measurements are taken of all injuries during autopsies, but ordinary photography projects a 3D wound on a 2D space. Using technologies such as photogrammetry, it is possible to create 3D detaile...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1499-9
更新日期：2017-05-01 00:00:00
abstract：:Qualitative information on the sequence composition of the allele and locus structure of the X-STRs DXS8378, DXS9898, DXS6789, GATA31E08, and GATA172D05 was generated in this study. Sequence data were obtained from chimpanzees (Pan troglodytes) and diverse human population groups including Africans, Caucasians, Asians...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0303-x
更新日期：2009-03-01 00:00:00
abstract：:When a Y-chromosomal and a (partial) autosomal DNA profile are obtained from one crime sample, and both profiles match the suspect's profiles, we would like to know the combined evidential value. To calculate the likelihood ratio of observing the autosomal and Y-chromosomal DNA profiles combined, we need to know the c...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-0971-7
更新日期：2014-11-01 00:00:00
abstract：:A chimera is an organism whose cells derive from two or more distinct zygote lineages. and therefore two different blood cell populations circulate in one individual. To point out the potential pitfalls in forensic analysis, a set of triplets (a girl and two boys) who revealed blood chimerism was investigated with fou...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050263
更新日期：1999-01-01 00:00:00
abstract：:Forensic identification comprises legal, social, ethical, and religious aspects where age detection is an important factor. When the case is a fetus or infant, recording various measurements of the body, head, and teeth is essential. The aim of this research is to evaluate the effects of different tooth and body measu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0935-3
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND:As sex estimation is an important step to delineate the biological profile, the development of tools employing anatomical structures which may maintain their integrity even after extreme events, such as the maxillary sinus, become useful for forensic identification. Thus, the aim in the present study was to ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1869-6
更新日期：2019-07-01 00:00:00
abstract：:Determining the time of injury is an important but still a challenging task in forensic anthropology. In literature, many descriptions can be found to make a distinction between perimortem and postmortem fractures. Characteristics that are more related to fractures in fresh conditions, however, are not extensively inv...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1514-1
更新日期：2017-05-01 00:00:00
abstract：:Population genetic studies were carried out on 932 caucasians from Austria using the short tandem repeat system ACTBP2 (SEE33). A sequenced allelic ladder was used for typing (Möller et al. 1995). After native polyacrylamide gel electrophoresis all 26 alleles of the ladder were found as well as 194 alleles which migra...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050111
更新日期：1998-01-01 00:00:00
