当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF LEGAL MEDICINE期刊下所有文献 > Technical note: EnVision™ FLEX improves the detectability of depletions of myoglobin and troponin T in forensic cases of myocardial ischemia/infarction.

Technical note: EnVision™ FLEX improves the detectability of depletions of myoglobin and troponin T in forensic cases of myocardial ischemia/infarction.

Abstract：

:Immunohistochemistry is a well-established technique used in many research laboratories as well as in clinical diagnostics. The method allows to visualize the expression of proteins in biological tissues, as well as to evaluate this expression semi-quantitatively. For diagnosis, an optimal staining, based on a straightforward protocol, is crucial. In many sudden cardiac death cases, immunohistochemistry is the only tool enabling the diagnosis of myocardial ischemia/infarction, thus the diagnosis of the cause of death. Improvements in immunoreactions are actually possible thanks to optimized detection systems. The recently introduced detection system EnVision Flex™ by Dako allows to dramatically improve (in terms of intensity of the signal and practically absence of background) the visualization of antigens in formalin-fixed paraffin-embedded (FFPE) tissue sections. We tested this method for the detection of myoglobin and troponin T in human postmortem cases of myocardial infarction, as the results obtained by using the « classical » ABC (avidin-biotin complex) method have proven to be sub-optimal, thus rendering any interpretation very difficult, if not impossible.

journal_name

Int J Legal Med

journal_title

International journal of legal medicine

authors

Sabatasso S,Pomponio C,Fracasso T

doi

10.1007/s00414-017-1575-9

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

1643-1646

issue

6

eissn

0937-9827

issn

1437-1596

pii

10.1007/s00414-017-1575-9

journal_volume

131

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF LEGAL MEDICINE文献大全
  • Insertion-deletion polymorphisms--utilization on forensic analysis.

    abstract：:Insertion-deletion (INDEL) markers are very frequent in the human genome and present several advantages for population and forensic studies, such as low mutation rates, easy interpretation, small amplicons, easy genotyping, and the possibility of using multiplex PCR. The great adaptability of INDELs for amplification ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-011-0588-z

    authors： da Costa Francez PA,Rodrigues EM,de Velasco AM,dos Santos SE

    更新日期：2012-07-01 00:00:00

  • Correction to: Age estimation based on pelvic ossification using regression models from conventional radiography.

    abstract：:The original version of this article unfortunately contained a mistake in the Results section. The correct equation from the cubic regression analysis for male is: Age = 14.408 + 0.475X-0.064X2 + 0.005X3, and for the female is: 18.196-1.637X + 0.204X2-0.005X3. The error doesn't affect the main conclusion. ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章,已发布勘误

    doi：10.1007/s00414-017-1751-y

    authors： Zhang K,Dong XA,Fan F,Deng ZH

    更新日期：2018-01-01 00:00:00

  • A comparison of 3H-cocaine binding on melanin granules and human hair in vitro.

    abstract：:The in vitro experiments on the interaction of 3H-cocaine and melanin from Sepia officinalis confirmed the existence of drug binding sites on melanin granules. The results suggested that the binding of 3H-cocaine to melanin could be analyzed by assuming that the binding to the surface of pigment granules is analogous ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s004140050031

    authors： Pötsch L,Skopp G,Rippin G

    更新日期：1997-01-01 00:00:00

  • Age estimation from the acetabulum in South African black males.

    abstract：:Anthropologists are constantly seeking to improve methods for age estimation in the human skeleton. A new method was introduced about a decade ago that assesses the morphological changes that take place in the acetabulum as an individual ages. The pelvis is usually well preserved in forensic cases, which makes this me...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-015-1299-7

    authors： Botha D,Pretorius S,Myburgh J,Steyn M

    更新日期：2016-05-01 00:00:00

  • Characterising the dynamics of expirated bloodstain pattern formation using high-speed digital video imaging.

    abstract：:During forensic investigations, it is often important to be able to distinguish between impact spatter patterns (blood from gunshots, explosives, blunt force trauma and/or machinery accidents) and bloodstain patterns generated by expiration (blood from the mouth, nose or lungs). These patterns can be difficult to dist...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-010-0498-5

    authors： Donaldson AE,Walker NK,Lamont IL,Cordiner SJ,Taylor MC

    更新日期：2011-11-01 00:00:00

  • Postmortem CT and MRI findings of massive fat embolism.

    abstract：OBJECTIVE:To elucidate postmortem computed tomography (PMCT) and postmortem magnetic resonance (PMMR) imaging findings suggesting massive fat embolism. MATERIALS AND METHODS:Consecutive forensic cases with PMCT and PMMR scans of subjects prior to autopsy were assessed. For PMCT, 16- or 64-row multidetector CT scans we...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-019-02128-8

    authors： Makino Y,Kojima M,Yoshida M,Motomura A,Inokuchi G,Chiba F,Torimitsu S,Hoshioka Y,Yamaguchi R,Saito N,Urabe S,Tsuneya S,Horikoshi T,Yajima D,Iwase H

    更新日期：2020-03-01 00:00:00

  • Comparative study on the effect of ethnicity on wisdom tooth eruption.

    abstract：:The current knowledge base for evaluating the influence of ethnic origin on wisdom tooth eruption is still inadequate. We therefore analyzed and compared the chronology of wisdom tooth eruption in three ethnic populations-German, Japanese, and black South African-based on evidence from 2,482 conventional orthopantomog...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-007-0171-9

    authors： Olze A,van Niekerk P,Ishikawa T,Zhu BL,Schulz R,Maeda H,Schmeling A

    更新日期：2007-11-01 00:00:00

  • Evaluation of the radiographic visibility of the root pulp in the lower third molars for the purpose of forensic age estimation in living individuals.

    abstract：:The question of whether an individual has reached the age of 18 is of crucial importance in forensic age estimation practice. In some countries, the age threshold of 21 years is relevant as well. A completed mineralization of third molars is not a sufficient criterion for a diagnosis of a minimum age of 18 years with ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-009-0415-y

    authors： Olze A,Solheim T,Schulz R,Kupfer M,Schmeling A

    更新日期：2010-05-01 00:00:00

  • Correction to: DNA typing from skeletal remains: a comparison between capillary electrophoresis and massively parallel sequencing platforms.

    abstract：:The name of an author was misspelled. The correct spelling is "Linlin Gao" instead of Linin Gao. ...

    journal_title：International journal of legal medicine

    pub_type： 已发布勘误

    doi：10.1007/s00414-020-02342-9

    authors： Liu Z,Gao L,Zhang J,Fan Q,Chen M,Cheng F,Li W,Shi L,Zhang X,Zhang J,Zhang G,Yan J

    更新日期：2020-11-01 00:00:00

  • Allele frequencies for 15 autosomal STR loci and haplotype data for 17 Y-STR loci in a population from Belize.

    abstract：:Allele frequencies for 15 autosomal STR loci (N = 290) and haplotype data for 17 Y-STR loci (N = 157) were determined for an admixed population from Belize. There were no detectable departures from Hardy-Weinberg equilibrium expectations at any autosomal STR loci except for the D8S1179 locus (p = 0.002). The combined ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1082-1

    authors： Flores S,Sun J,King J,Eisenberg A,Budowle B

    更新日期：2015-11-01 00:00:00

  • Intravenous methadone application as a serious risk factor for an overdose death: methadone-related fatalities in Hamburg from 2007 to 2012.

    abstract：:Methadone plays an increasing role in drug-related deaths in Hamburg. To find out whether intravenous application of methadone plays a relevant role in methadone-related deaths, body fluids of all methadone-positive cases (n=130) and three buprenorphine-positive cases where a urine sample was available (n=58+3) were i...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1017-x

    authors： Iwersen-Bergmann S,Jungen H,Andresen-Streichert H,Müller A,Elakkary S,Püschel K,Heinemann A

    更新日期：2014-09-01 00:00:00

  • Drying properties of bloodstains on common indoor surfaces.

    abstract：:When blood reaches an extracorporeal surface, a drying process is initiated. Properties of this drying process may be crucial for the correct assessment of case-specific time lapses, however, there is a lack of systematic studies concerning the drying times of blood. We present a study on drying properties of small bl...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-012-0734-2

    authors： Ramsthaler F,Schmidt P,Bux R,Potente S,Kaiser C,Kettner M

    更新日期：2012-09-01 00:00:00

  • Radiographic evaluation of Gustafson's criteria for the purpose of forensic age diagnostics.

    abstract：:The main criteria used in dental age estimation in living young individuals are mineralisation and eruption of the third molars. In order to further diversify the spectrum of characteristics after completion of third molar development, tests were undertaken to determine whether the characteristics studied by Gustafson...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-012-0701-y

    authors： Olze A,Hertel J,Schulz R,Wierer T,Schmeling A

    更新日期：2012-07-01 00:00:00

  • Lethal thyroid storm after uncontrolled intake of liothyronine in order to lose weight.

    abstract：:Thyroid hormones are sometimes used for purposes for which they are not approved. Reasons for off-label use can be overweight, prevailing depressive mood, or various somatic symptoms. Information about the intake of thyroid hormones in order to lose weight can be easily obtained from inappropriate/nonmedical websites....

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-010-0423-y

    authors： Hartung B,Schott M,Daldrup T,Ritz-Timme S

    更新日期：2010-11-01 00:00:00

  • How taphonomic alteration affects the detection and imaging of striations in stab wounds.

    abstract：:Stabbing with a kitchen knife is a common method of homicide in Europe. Serrated knives may leave tool markings (striations) in tissues. Documentation of striations is necessary for their use as forensic evidence. Traditional methods (physical casting and photography) have significant limitations, and micro-computed t...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-017-1715-2

    authors： Stanley SA,Hainsworth SV,Rutty GN

    更新日期：2018-03-01 00:00:00

  • Molecular analysis of different classes of RNA molecules from formalin-fixed paraffin-embedded autoptic tissues: a pilot study.

    abstract：:For a long time, it has been thought that fresh and frozen tissues are the only possible source of biological material useful to extract nucleic acids suitable for downstream molecular analysis. Recently, for forensic purpose such as personal identification, also fixed tissues have been used to recover DNA molecules, ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1066-1

    authors： Muciaccia B,Vico C,Aromatario M,Fazi F,Cecchi R

    更新日期：2015-01-01 00:00:00

  • Investigation of phase II metabolism of 11-hydroxy-Δ-9-tetrahydrocannabinol and metabolite verification by chemical synthesis of 11-hydroxy-Δ-9-tetrahydrocannabinol-glucuronide.

    abstract：:(-)-Δ-9-tetrahydrocannabinol ((-)-Δ-9-THC) is the main psychoactive constituent in cannabis. During phase I metabolism, it is metabolized to (-)-11-hydroxy-Δ-9-tetrahydrocannabinol ((-)-11-OH-Δ-9-THC), which is psychoactive, and to (-)-11-nor-9-carboxy-Δ-9-tetrahydrocannabinol ((-)-Δ-9-THC-COOH), which is psychoinacti...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-020-02387-w

    authors： Hassenberg C,Clausen F,Hoffmann G,Studer A,Schürenkamp J

    更新日期：2020-11-01 00:00:00

  • Bite through the tent.

    abstract：:The authors report on a young boy who was bitten into his face by an unknown animal while being asleep in a tent. Given the bite marks and the location of the scene, members of the mustelidae and canidae families were the first "suspects." Deoxyribunucleic acid (DNA) recovered from the tent's wall was analyzed with re...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-012-0674-x

    authors： Naue J,Lutz-Bonengel S,Pietsch K,Sänger T,Schlauderer N,Schmidt U

    更新日期：2012-05-01 00:00:00

  • Population data and phylogenetic structure of Han population from Jiangsu province of China on GlobalFiler STR loci.

    abstract：:Forensic statistical parameters based on allelic frequencies of commonly used short tandem repeats were estimated for the Han population of Jiangsu province from P.R. China. The 6-dye GlobalFiler™ PCR amplification kit incorporates 21 autosomal STRs, providing reliable DNA typing results with enhanced the power of dis...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-018-1815-7

    authors： Adnan A,Zhan X,Kasim K,Rakha A,Xin XJ

    更新日期：2018-09-01 00:00:00

  • Sudden unexpected death in childhood due to eosinophilic myocarditis.

    abstract：:A 12-year-old boy with no previous serious medical history experienced abdominal discomfort and chest pains for 5 days and suddenly died. The autopsy revealed diffuse and extensive infiltration of eosinophils into the myocardium, with poorly formed granulomas and few fibrotic changes. The necrotic changes was so exten...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/BF01369797

    authors： Aoki Y,Nata M,Hashiyada M,Sagisaka K

    更新日期：1996-01-01 00:00:00

  • Crack propagation through sandwich bones due to low-velocity projectile impact.

    abstract：:Projectile impact in sandwich bones typically results in formation of conoidal wounds exhibiting a larger region of damage on the inner cortical plate termed the bevel. To date, a number of hypotheses have been put forward to explain the formation of this wound type. The plug and spall hypothesis suggests that the con...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-019-02086-1

    authors： Rickman JM,Shackel J

    更新日期：2019-09-01 00:00:00

  • First Polish DNA "manhunt"--an application of Y-chromosome STRs.

    abstract：:This study presents the application of Y-chromosomal STR polymorphisms to male identification in the case of a serial rapist and woman murderer in Poland. Since August 1996 a rapist from Swinoujscie (northwest Poland) committed at least 14 rapes. In the year 2000 he brutally raped 8 young girls and murdered a 22-year-...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-002-0320-0

    authors： Dettlaff-Kakol A,Pawlowski R

    更新日期：2002-10-01 00:00:00

  • A new "miniSTR-multiplex" displaying reduced amplicon lengths for the analysis of degraded DNA.

    abstract：:A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly de...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-005-0013-6

    authors： Grubwieser P,Mühlmann R,Berger B,Niederstätter H,Pavlic M,Parson W

    更新日期：2006-03-01 00:00:00

  • Forensic reconstruction of two military combat related shooting incidents using an anatomically correct synthetic skull with a surrogate skin/soft tissue layer.

    abstract：:Six synthetic head models wearing ballistic protective helmets were used to recreate two military combat-related shooting incidents (three per incident, designated 'Incident 1' and 'Incident 2'). Data on the events including engagement distances, weapon and ammunition types was collated by the Defence Science and Tech...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-018-1802-z

    authors： Mahoney P,Carr D,Harrison K,McGuire R,Hepper A,Flynn D,Delaney RJ,Gibb I

    更新日期：2019-01-01 00:00:00

  • Development of a five ChX STRs loci typing system.

    abstract：:We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up....

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-008-0223-9

    authors： Liu QL,Lv DJ,Wu XL,Sun HY,Wu XY,Lu HL

    更新日期：2008-05-01 00:00:00

  • A new method for ABO genotyping to avoid discrepancy between genetic and serological determinations.

    abstract：:In Japan and elsewhere, ABO genotyping is frequently used in forensic practice for identification of a decomposed body. However, the phenotype deduced from the genotyping data is occasionally inconsistent with the real phenotype. In this paper, we report a simple ABO genotyping method in which five single nucleotide p...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-006-0148-0

    authors： Shintani-Ishida K,Zhu BL,Maeda H,Uemura K,Yoshida K

    更新日期：2008-01-01 00:00:00

  • PCR-based typing of DNA extracted from cigarette butts.

    abstract：:Limited genetic marker information can be obtained from saliva by typing by conventional serological means. Thus, the application of PCR-based DNA typing methods was investigated as a potential approach for typing genetic markers in saliva. DNA was isolated from 200 cigarettes smoked by 10 different individuals (20 ci...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/BF01369812

    authors： Hochmeister MN,Budowle B,Jung J,Borer UV,Comey CT,Dirnhofer R

    更新日期：1991-01-01 00:00:00

  • Allele frequency data of 15 autosomal STR loci in four major population groups of South Africa.

    abstract：:Allele frequency distributions for 15 tetrameric short tandem repeat (STR) loci were determined using the AmpFlSTR® Identifiler Plus™ PCR amplification kit. There was little evidence of departures from Hardy-Weinberg equilibrium or association of alleles of different loci in the population samples. The probability of ...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-013-0898-4

    authors： Lucassen A,Ehlers K,Grobler PJ,Shezi AL

    更新日期：2014-03-01 00:00:00

  • Sex determination from buccal mucosa scrapes.

    abstract：:Establishing individuality is an imperative aspect in any investigation procedure. At times, it becomes necessary to determine the sex of the individual to establish identity, and saliva stains found at the scene of crime are of major help in such cases. In the present study, we have determined the sex of the individu...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-008-0285-8

    authors： Mittal T,Saralaya KM,Kuruvilla A,Achary C

    更新日期：2009-09-01 00:00:00

  • Species identification from hair by means of spectral library searches.

    abstract：:Species identification from hair has been performed in the past by several techniques, such as scanning electron microscopy and polymerase chain reaction. Despite the great promise of mass spectrometry herein, the repetitive glycine stretches and the evolutionary conserved sequences of keratins make the results from c...

    journal_title：International journal of legal medicine

    pub_type： 杂志文章

    doi：10.1007/s00414-014-1008-y

    authors： Van Steendam K,De Wulf O,Dhaenens M,Deforce D

    更新日期：2014-09-01 00:00:00