Abstract：
:Limited genetic marker information can be obtained from saliva by typing by conventional serological means. Thus, the application of PCR-based DNA typing methods was investigated as a potential approach for typing genetic markers in saliva. DNA was isolated from 200 cigarettes smoked by 10 different individuals (20 cigarettes per individual) and from 3 cigarette butts recovered from 2 crime scenes (adjudicated cases) using a Chelex 100 extraction procedure. The amount of recovered human DNA was quantified by slot-blot analysis and ranged from approximately less than 2-160 ng DNA per cigarette butt for the 200 samples, and 8 ng, 50 ng, and 100 ng for the cigarette butts from the adjudicated cases. The DNA was successfully amplified by the polymerase chain reaction (PCR) for the HLA-DQ alpha locus (99 out of 100 samples) as well as for the variable number of tandem repeat (VNTR) locus D1S80 (99 out of 100 samples). Amplification and typing of DNA was successful on all samples recovered from the crime scenes. The results suggest that PCR-based typing of DNA offers a potential method for genetically characterizing traces of saliva on cigarette butts.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Hochmeister MN,Budowle B,Jung J,Borer UV,Comey CT,Dirnhofer Rdoi
10.1007/BF01369812subject
Has Abstractpub_date
1991-01-01 00:00:00pages
229-33issue
4eissn
0937-9827issn
1437-1596journal_volume
104pub_type
杂志文章abstract：:Five short tandem repeat (STR) systems HumVWA, HumTH01, HumCD4, HumF13B and HumFES were investigated in 2 subpopulations living in Turkey (Laz Turks and Kurds). The population genetic data were compared to a Turkish population sample from the Adana area. A closer genetic relationship was found to the Laz Turks than to...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050059
更新日期：1997-01-01 00:00:00
abstract：:In order to establish a reliable age indicator in the period when all other epiphyseal age indicators have already been inactivated, medial clavicle as the bone with the longest period of growth became the object of various investigations. However, the lack of population-specific method often made it unreliable in som...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0791-6
更新日期：2013-05-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02456-0
更新日期：2020-12-19 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004149900111
更新日期：2001-01-01 00:00:00
abstract：:The performance of two detection techniques for body fluids, the Spectra-Physics(R) Revealtrade mark portable forensic laser system and the mercury-arc lamp Lumatec Superlite 400, was evaluated with various biological stains on different substrates. Serial dilutions of neat, 1/10, 1/100 and 1/1,000 using fluid semen, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-007-0214-2
更新日期：2008-05-01 00:00:00
abstract：:The utility of postmortem microbiology has continuously been a topic of controversy. The present study describes a case of fatal sepsis in a patient with systemic lupus erythematosus. Postmortem culture and genotyping analyses allowed us to identify Klebsiella pneumoniae as the cause of sepsis, revealing the inadequat...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1160-z
更新日期：2015-09-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
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更新日期：2007-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0308-5
更新日期：2010-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
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更新日期：2000-01-01 00:00:00
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journal_title：International journal of legal medicine
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更新日期：2002-08-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1288-x
更新日期：2016-05-01 00:00:00
abstract：:Genetic data from 21 autosomal insertion-null (INNULs) markers found in the InnoTyper® 21 Human DNA Analysis (InnoGenomics®) was evaluated in 190 unrelated individuals from Andalusia. Allele frequencies and forensic parameters were estimated for the 20 INNULs. All loci were in Hardy-Weinberg equilibrium in the study p...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02044-x
更新日期：2019-09-01 00:00:00
abstract：:Despite being a common form of abuse, there is a paucity of literature describing shackling and wrist restraint injuries among survivors of torture. Forensic evaluation of alleged wrist restraint/handcuff injuries in survivors of torture presents challenges to the evaluator, especially if the injuries are remote and d...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02451-5
更新日期：2021-01-07 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-002-0320-0
更新日期：2002-10-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004149900099
更新日期：2000-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0285-8
更新日期：2009-09-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02023-2
更新日期：2020-03-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0243-5
更新日期：2008-09-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050080
更新日期：1997-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2001-08-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2021-01-27 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
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更新日期：2006-07-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01247266
更新日期：1994-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225046
更新日期：1993-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
abstract：:We developed two multiplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-Multiplex 1 consisted of DXS6807, DXS6800, DXS7424, DXS101, GATA172D05 and HPRTB and X-Multiplex 2 consisted of DXS8378, DXS9898, DXS6801, DXS6809, DXS6789, DXS7133, DXS8377 and DXS7423. In addition, we present a...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-011-0629-7
更新日期：2012-03-01 00:00:00
