Abstract：
:A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly degraded DNA from casework samples, which had shown no or only poor results for these loci in previous analyses with standard primer sets. The application of the new miniSTR-multiplex resulted in an increased overall typing success rate for degraded DNA samples. In a concordance study between the conventional and the newly designed primers, no genotype differences were revealed in 124 randomly selected individuals.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Grubwieser P,Mühlmann R,Berger B,Niederstätter H,Pavlic M,Parson Wdoi
10.1007/s00414-005-0013-6subject
Has Abstractpub_date
2006-03-01 00:00:00pages
115-20issue
2eissn
0937-9827issn
1437-1596journal_volume
120pub_type
杂志文章abstract：:Mitragyna speciosa (Rubiaceae), commonly known as kratom, is a tropical tree with a long history of traditional use in parts of Africa and Southeast Asia. In recent years, kratom has gained popularity for use as a recreational drug across the globe. Relatively new to the illicit market and used in a manner different f...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-015-1279-y
更新日期：2016-01-01 00:00:00
abstract：:Blood, saliva, and semen are some of the forensically most relevant biological stains commonly found at crime scenes, which can often be of small size or challenging due to advanced decay. In this context, it is of great importance to possess reliable knowledge about the effects of degradation under different environm...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1373-9
更新日期：2016-11-01 00:00:00
abstract：:The problems of testing patients or the deceased using classical blood group systems for identity or paternity purposes after multiple transfusions are well known. In a paternity case where the putative father received multiple transfusions it was possible to determine some classical blood group systems. The investiga...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01371333
更新日期：1994-01-01 00:00:00
abstract：:We developed two multiplex systems for the coamplification of X-chromosomal short tandem repeats (STRs). X-Multiplex 1 consisted of DXS6807, DXS6800, DXS7424, DXS101, GATA172D05 and HPRTB and X-Multiplex 2 consisted of DXS8378, DXS9898, DXS6801, DXS6809, DXS6789, DXS7133, DXS8377 and DXS7423. In addition, we present a...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-011-0629-7
更新日期：2012-03-01 00:00:00
abstract：:For a long time, it has been thought that fresh and frozen tissues are the only possible source of biological material useful to extract nucleic acids suitable for downstream molecular analysis. Recently, for forensic purpose such as personal identification, also fixed tissues have been used to recover DNA molecules, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1066-1
更新日期：2015-01-01 00:00:00
abstract：:The purpose of this investigation was to determine the cause of death of a 13-year-old girl, where none was immediately evident. Our analysis showed it to be a very unusual case of a drug-facilitated sexual assault (DFSA), which led to the tragic death of the young rape victim and then to the suicide of the rapist. Th...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-005-0020-7
更新日期：2006-07-01 00:00:00
abstract：:Crania are a reliable source for sex estimation in Euro-Americans, Europeans, and most other populations. Besides morphological assessments, the application of Fordisc® has become a useful tool within the last two decades, creating discriminant functions from morphometric data. Unfortunately, until now, white populati...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1469-2
更新日期：2017-07-01 00:00:00
abstract：:Inferring an unknown DNA's ancestry using a set of ancestry-informative single nucleotide polymorphisms (SNPs) in forensic science is useful to provide investigative leads. This is especially true when there is no DNA database match or specified suspect. Thus, a set of SNPs with highly robust and balanced differential...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1863-z
更新日期：2019-07-01 00:00:00
abstract：:Qualitative information on the sequence composition of the allele and locus structure of the X-STRs DXS8378, DXS9898, DXS6789, GATA31E08, and GATA172D05 was generated in this study. Sequence data were obtained from chimpanzees (Pan troglodytes) and diverse human population groups including Africans, Caucasians, Asians...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0303-x
更新日期：2009-03-01 00:00:00
abstract：:We analysed 15 autosomal STRs in 200 unrelated individuals (102 males and 98 females) and 17 Y-STRs in 102 unrelated males living in Jharkhand, India, to establish parameters of forensic interest. The examined autosomal STRs revealed high combined power of exclusion (CPE) and combined power of discrimination (CPD) as ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1610-x
更新日期：2018-01-01 00:00:00
abstract：:Allele frequencies at the short tandem repeat (STR) loci HumACTBP2 and HumVWA were determined in 118 unrelated individuals from Northern Italy (Milan area). For locus HumACTBP2 (SE33) a total of 39 alleles was observed. Furthermore, two interalleles (N18m+N19m) and one allele (> N35) were found which were not observed...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050090
更新日期：1997-01-01 00:00:00
abstract：:One of the most common conditions during fetal development is anencephaly, which often involves many identification difficulties in the context of physical anthropology, as it causes severe skull challenges. In this paper, we describe the alterations found in the skulls of two perinatal individuals with anencephaly fr...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1123-9
更新日期：2015-07-01 00:00:00
abstract：:When a Y-chromosomal and a (partial) autosomal DNA profile are obtained from one crime sample, and both profiles match the suspect's profiles, we would like to know the combined evidential value. To calculate the likelihood ratio of observing the autosomal and Y-chromosomal DNA profiles combined, we need to know the c...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-0971-7
更新日期：2014-11-01 00:00:00
abstract：:The performance of two detection techniques for body fluids, the Spectra-Physics(R) Revealtrade mark portable forensic laser system and the mercury-arc lamp Lumatec Superlite 400, was evaluated with various biological stains on different substrates. Serial dilutions of neat, 1/10, 1/100 and 1/1,000 using fluid semen, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-007-0214-2
更新日期：2008-05-01 00:00:00
abstract：:Expert witness opinion based on the comparison of images has been accepted by UK courts as admissible evidence in relation to issues of identity. Within images of the hand are a multiplicity of anatomical features of different aetiology, incidence and distribution patterns and this includes melanocytic nevi, referred ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0821-z
更新日期：2014-05-01 00:00:00
abstract：:To increase the reliability of forensic age estimations in living adolescents and young adults where there is no legitimation for X-ray examinations, it would be desirable to establish radiation-free imaging methods. In the present study, the ossification stage of the distal femoral epiphysis was determined retrospect...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-0967-3
更新日期：2014-05-01 00:00:00
abstract：:The smoking cessation agent varenicline acts as a partial agonist on α(4)β(2) nicotinic acetylcholine receptors. Although debated, several reports have linked varenicline therapy to an increased risk of suicidal thoughts and/or suicide. In addition, several non-fatal overdose cases have been reported. In this report, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0695-5
更新日期：2013-01-01 00:00:00
abstract：:Suppose that part of the prosecution's evidence in some crime case is analysis of a blood stain, and that the traits E discovered in the stain suggest multiple donors. Then the prosecution will probably allege some specific inculpatory hypothesis H0 about the sources of the stain, and P (E [symbol: see text] H0) can b...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225523
更新日期：1996-01-01 00:00:00
abstract：:The histological staining technique using rhodizonate is also effective for the determination of the firing distance by examining the distribution and intensity of the staining reaction. The differentiation between absolute close-range shots and long-range shots is generally possible without any doubt. The method is n...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100220
更新日期：2002-02-01 00:00:00
abstract：:We performed a population study on 15 polymorphic STR loci (FH2010, FH2079, PEZ2, VWF.X, FH2054, FH2087Ub, FH2611, WILMS-TF, PEZ12, PEZ15, PEZ6, FH2087Ua, ZUBECA4, ZUBECA6, FH2132) on 131 randomly selected dogs. Alleles were identified and grouped according to their estimated fragment length using fixed allelic bins e...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-004-0452-5
更新日期：2004-10-01 00:00:00
abstract：:The morphological structure and immunohistochemical reactions of 100 pituitaries from cases of SIDS children (58 males and 42 females, average age 5.34 +/- 3.12 months) were studied. Controls consisted of 19 pituitaries from children (14 males and 5 females, average age 5.63 +/- 2.52 months) with a clearly identifiabl...
journal_title：International journal of legal medicine
pub_type： 杂志文章,多中心研究
doi：10.1007/BF01225414
更新日期：1994-01-01 00:00:00
abstract：:The name of an author was misspelled. The correct spelling is "Linlin Gao" instead of Linin Gao. ...
journal_title：International journal of legal medicine
pub_type： 已发布勘误
doi：10.1007/s00414-020-02342-9
更新日期：2020-11-01 00:00:00
abstract：:Allele frequencies for 15 autosomal STR loci (N = 290) and haplotype data for 17 Y-STR loci (N = 157) were determined for an admixed population from Belize. There were no detectable departures from Hardy-Weinberg equilibrium expectations at any autosomal STR loci except for the D8S1179 locus (p = 0.002). The combined ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1082-1
更新日期：2015-11-01 00:00:00
abstract：:Frontal sinuses are of special interest for personal identification thanks to their high variability. A common procedure for comparing ante-mortem and post-mortem material is based on coding systems classifying frontal sinuses according to their morphological and metrical characteristics. However, the calculation of p...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02293-1
更新日期：2020-09-01 00:00:00
abstract：:Cyanide (CN) blood concentration is hardly considered during routine when evaluating smoke gas intoxications and fire victims, although some inflammable materials release a considerable amount of hydrogen cyanide. CN can be significant for the capacity to act and can in the end even be the cause of death. Systematic d...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1426-0
更新日期：2017-01-01 00:00:00
abstract：:We propose that clusters of closely linked markers, which segregate as stable haplotypes, provide a high potential to solve complex kinship cases. It is known that the X-chromosomal centromere region shows an extremely low degree of recombination. Hence, we focused our interest on the region between 56 and 64 Mb dista...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-009-0328-9
更新日期：2010-01-01 00:00:00
abstract：:Linking biological samples found at a crime scene with the actual crime event represents the most important aspect of forensic investigation, together with the identification of the sample donor. While DNA profiling is well established for donor identification, no reliable methods exist for timing forensic samples. He...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0457-1
更新日期：2010-09-01 00:00:00
abstract：:An obese man (height, 178 cm; weight, 160 kg; body mass index, 50.5 kg/m2) with a history of recurrent ventricular tachycardia (VT), cardiomyopathy, coronary sclerosis, and insulin-resistant diabetes suffered a right femur fracture in a vehicular accident. His fracture was repaired the following day, but shortly after...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1392-6
更新日期：2016-11-01 00:00:00
abstract：:Thyroid hormones are sometimes used for purposes for which they are not approved. Reasons for off-label use can be overweight, prevailing depressive mood, or various somatic symptoms. Information about the intake of thyroid hormones in order to lose weight can be easily obtained from inappropriate/nonmedical websites....
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-010-0423-y
更新日期：2010-11-01 00:00:00
abstract：:The level of exposure or concealment of a cadaver is known to have an important impact on insect colonisation and decomposition but has been the subject of few investigations. In the present study, 30 pig carcasses were stored in three different types of containers (suitcases, trashcans and drums) with different level...
journal_title：International journal of legal medicine
pub_type：
doi：10.1007/s00414-019-02089-y
更新日期：2019-09-01 00:00:00
