Abstract:
:The identification of species of the forensically important genus Sarcophaga is very difficult and requires strong taxonomic expertise. In this study, we sequenced the mitochondrial cytochrome c oxidase subunit I (COI) gene of 126 specimens of 56 W European Sarcophaga species and added GenBank data to our database to yield a total dataset of 270 COI sequences from 99 Sarcophaga species to evaluate the COI gene as a molecular diagnostic tool for species identification in this genus. Using two simple criteria (Best Match, BM and Best Close Match, BCM), we showed that the identification success using a mini-barcode region of 127 bp was very low (80.7-82.5 %) and the use of this region is not recommended as a species identifier. In contrast, identification success was very high using the standard barcode region (658 bp) or using the entire COI region (1,535 bp) (98.2-99.3 %). Yet, there was a low interspecific sequence divergence (<2 %) in six species groups so that for 16 out of the 99 species (nine of which are of forensic importance), the use of COI barcodes as species identifier should be done with care. For these species, additional markers will be necessary to achieve a 100 % identification success. We further illustrate how such reference databases can improve local reference databases for forensic entomologists.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Jordaens K,Sonet G,Richet R,Dupont E,Braet Y,Desmyter Sdoi
10.1007/s00414-012-0767-6subject
Has Abstractpub_date
2013-03-01 00:00:00pages
491-504issue
2eissn
0937-9827issn
1437-1596journal_volume
127pub_type
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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更新日期:2010-09-01 00:00:00
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