Abstract：
:Gene expression analyses based on messenger RNA (mRNA) profiling require accurate data normalisation. When using endogenous reference genes, these have to be validated carefully. Therefore, we examined the transcript stability of 10 potential reference genes using quantitative real-time polymerase chain reaction: beta actin, 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase, TATA box-binding protein, hypoxanthine phosphoribosyl-transferase I, beta-2-microglobulin, hydroxymethylbilane synthase, succinate dehydrogenase complex, subunit A, cyclophilin A and ubiquitin C. The aim of the current study was to assess which reference genes show stable mRNA levels in human post mortem cardiac muscle, skeletal muscle and brain tissue. Considering cardiac muscle tissue, CYCA and TBP were identified as the most stable while in skeletal muscle tissue, SDHA and TBP, and in brain tissue, SDHA and HMBS turned out to be the most stable. Furthermore, we recommend a minimum of four carefully validated endogenous control genes for reliable data normalisation in human post mortem tissue. Parameters influencing the stability of transcript amounts were found to be mainly the post mortem interval in cardiac muscle and skeletal muscle tissue and the donor's cause of death in skeletal muscle and brain samples. Further parameters like gender, age at death and body mass index were found to influence mRNA quantities in skeletal muscle only. The set of stable control genes identified in this study may be used in further studies if the composition of the samples is similar to the one used here.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Koppelkamm A,Vennemann B,Fracasso T,Lutz-Bonengel S,Schmidt U,Heinrich Mdoi
10.1007/s00414-010-0433-9subject
Has Abstractpub_date
2010-09-01 00:00:00pages
371-80issue
5eissn
0937-9827issn
1437-1596journal_volume
124pub_type
杂志文章abstract：:This study demonstrates post-mortem autolytic alterations in the skin at cellular and subcellular levels and identifies parameters which may assist in determining the time of death in the first few hours post-mortem. Serial skin samples from the ventral surface of the arm were taken at intervals of 3, 6, 9 and 12 h af...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01225600
更新日期：1994-01-01 00:00:00
abstract：:The purpose of this study was to explore the applicability of periodontal ligament visibility (PLV) at the 18-year threshold. This mandibular maturity marker is graded into four separate age related stages, PLV-A, PLV-B, PLV-C, and PLV-D. These are discernible on a dental panoramic tomograph (DPT). The sample comprise...
journal_title：International journal of legal medicine
pub_type： 杂志文章
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-004-0506-8
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0935-3
更新日期：2014-05-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1049-2
更新日期：2015-03-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0674-x
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1259-2
更新日期：2016-03-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0817-8
更新日期：2013-09-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0105-y
更新日期：2006-11-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-009-0328-9
更新日期：2010-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1926-1
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140000158
更新日期：2000-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0713-7
更新日期：2013-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 传,历史文章,杂志文章
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更新日期：2003-04-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-013-0841-8
更新日期：2013-05-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100225
更新日期：2001-10-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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journal_title：International journal of legal medicine
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journal_title：International journal of legal medicine
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journal_title：International journal of legal medicine
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journal_title：International journal of legal medicine
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journal_title：International journal of legal medicine
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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journal_title：International journal of legal medicine
pub_type： 杂志文章
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