Abstract：
:Rapid identification of human remains following mass casualty events is essential to bring closure to family members and friends of the victims. Unfortunately, disaster victim identification, missing persons identification, and forensic casework analysis are often complicated by sample degradation due to exposure to harsh environmental conditions. Following a mass disaster, forensic laboratories may be overwhelmed by the number of dissociated portions that require identification and reassociation or compromised by the event itself. The interval between the disaster and receipt of victim samples at a laboratory is critical in that sample quality deteriorates as the postmortem interval increases. When bodies decompose due to delay in collection, transport, and sample processing, DNA becomes progressively fragmented, adversely impacting identification. We have previously developed a fully automated, field-forward Rapid DNA identification system that produces STR profiles (also referred to as DNA IDs or DNA fingerprints) from buccal and crime scene samples. The system performs all sample processing and data interpretation in less than 2 h. Here, we present results on Rapid DNA identification performed on several tissue types (including buccal, muscle, liver, brain, tooth, and bone) from exposed human bodies placed above ground or stored in a morgue/cooler, two scenarios commonly encountered following mass disasters. We demonstrate that for exposed remains, buccal swabs are the sample of choice for up to 11 days exposure and bone and tooth samples generated excellent DNA IDs for the 1-year duration of the study. For refrigerated remains, all sample types generated excellent DNA IDs for the 3-month testing period.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Turingan RS,Brown J,Kaplun L,Smith J,Watson J,Boyd DA,Steadman DW,Selden RFdoi
10.1007/s00414-019-02186-ysubject
Has Abstractpub_date
2020-05-01 00:00:00pages
863-872issue
3eissn
0937-9827issn
1437-1596pii
10.1007/s00414-019-02186-yjournal_volume
134pub_type
杂志文章abstract：:Population genetic studies were carried out on randomly selected and unrelated healthy individuals from Sicily (n = 140-150 individuals) using the short tandem repeat (STR) systems HumTH01, HumvWA31, HumFESFPS and HumF13A01. After vertical electrophoresis on polyacrylamide denaturing gels 6 alleles could be identified...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050171
更新日期：1998-01-01 00:00:00
abstract：:In humans, the amelogenin gene is present on both the X and the Y chromosomes. However, there are size differences in this gene between these chromosomes, which have been utilised for sexing in forensic casework and prenatal diagnosis. Our study using the AmpFl STR Profiler Plus kit, showed a deletion of Y chromosome-...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-001-0262-y
更新日期：2002-04-01 00:00:00
abstract：:Fatal head trauma (FHT) represents one of the most frequent causes of death diagnosed in forensic pathology. However, profound statistic autopsy data on FHT is still sparse. Therefore, the purpose of this study was to investigate the circumstances and injury patterns of FHT with particular focus on age and sex, and ad...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02187-x
更新日期：2020-01-01 00:00:00
abstract：:A population database was generated from 118 unrelated Caucasoid individuals living in Spain. Sequence polymorphisms of the mitochondrial DNA (mtDNA) control region, hypervariable regions I and II (HVRI and HVRII) were determined using the polymerase chain reaction (PCR) and direct sequencing. A total of 102 different...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140000158
更新日期：2000-01-01 00:00:00
abstract：:STR-based individualisation of biological deposits on bullets after perforation of tissue, can identify the person injured or killed by a particular bullet and comparison with the firearms used can identify the weapon and thus possibly the person who did the shooting. In this study, the effect of subsequent impacts on...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140000203
更新日期：2001-01-01 00:00:00
abstract：:In order to establish a reliable age indicator in the period when all other epiphyseal age indicators have already been inactivated, medial clavicle as the bone with the longest period of growth became the object of various investigations. However, the lack of population-specific method often made it unreliable in som...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0791-6
更新日期：2013-05-01 00:00:00
abstract：:This paper investigates the effects that different summary statistics (minimum, median, mean, or maximum), temporal sampling resolutions (duration between sampling events), and sample sizes (number of individuals sampled per sampling event) had on the accuracy and precision of the regression coefficients of a typical ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0243-5
更新日期：2008-09-01 00:00:00
abstract：:Bee and wasp stings represent an uncommon cause of death. In a retrospective study of all fatalities due to venomous animals in Sweden during a 10-year-period, we found 19 fatalities due to wasps, 1 due to bees but none due to snake bite. This gives an annual incidence of 0.2 per million inhabitants. Most of the decea...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01626039
更新日期：1991-03-01 00:00:00
abstract：:Myocardial infarctions go along with biomechanical stress, i.e. stretching of muscle fibres, and the expression of certain marker molecules. We tested if two of those markers, endothelin-1 (ET-1) and growth differentiation factor 15 (GDF-15), can be used as immunohistochemical markers for myocardial ischaemia/infarcti...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1726-z
更新日期：2018-03-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1193-3
更新日期：2015-07-01 00:00:00
abstract：:Sequence variation of the hypervariable segments (HVS) I/II of mitochondrial DNA (mtDNA) and the haplogroup affiliation were determined in a sample of 271 Italian subjects. This analysis showed that 42% of the individuals could be ascribed to H, the most frequent haplogroup in European Caucasian populations. This frac...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-005-0059-5
更新日期：2006-05-01 00:00:00
abstract：:Reports on penetrating ballistic head injuries in the literature are dominated by case studies of suicides; the penetrating ammunition usually being .22 rimfire or shotgun. The dominating cause of injuries in modern warfare is fragmentation and hence, this is the primary threat that military helmets protect the brain ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1073-2
更新日期：2015-05-01 00:00:00
abstract：:The alcoholism marker CDT was determined on 257 blood samples 1-2 days after the blood samples were taken and again after storage for 7 months at +4 degrees C. The differences between the pairs of CDT values were so large that the determination of CDT after long term storage of the blood sample has no evidential value...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050013
更新日期：2000-01-01 00:00:00
abstract：:A method has been developed for the simultaneous determination of lysergic acid diethylamide (LSD), its epimer iso-LSD, and its main metabolites nor-LSD and 2-oxo-3-hydroxy LSD in blood, urine, and, for the first time, vitreous humor samples. The method is based on liquid/liquid extraction and liquid chromatography-mu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0078-x
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVE:To elucidate postmortem computed tomography (PMCT) and postmortem magnetic resonance (PMMR) imaging findings suggesting massive fat embolism. MATERIALS AND METHODS:Consecutive forensic cases with PMCT and PMMR scans of subjects prior to autopsy were assessed. For PMCT, 16- or 64-row multidetector CT scans we...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02128-8
更新日期：2020-03-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-005-0053-y
更新日期：2007-01-01 00:00:00
abstract：:Current analysis of forensic DNA stains relies on the probabilistic interpretation of bulk-processed samples that represent mixed profiles consisting of an unknown number of potentially partial representations of each contributor. Single-cell methods, in contrast, offer a solution to the forensic DNA mixture problem b...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-021-02503-4
更新日期：2021-01-23 00:00:00
abstract：:A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly de...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-005-0013-6
更新日期：2006-03-01 00:00:00
abstract：:Single nucleotide polymorphism (SNP) typing offers promise to forensic genetics. Various strategies and panels for analyzing SNP markers for individual identification have been published. However, the best panels with fewer identity SNPs for all major population groups are still under discussion. This study aimed to f...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1250-y
更新日期：2016-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0734-2
更新日期：2012-09-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050156
更新日期：1998-01-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1342-3
更新日期：2016-11-01 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1377-5
更新日期：2016-11-01 00:00:00
abstract：:The aim of this study was to measure catecholamines and their O-methylated metabolites in urine and vitreous humor collected in cardiac deaths and noncardiac control cases that underwent medicolegal investigations. Our first goal was to assess whether cardiac events of different types are characterized by different ca...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1303-2
更新日期：2016-07-01 00:00:00
abstract：:A publically available database of the most highly cited scientists in all disciplines was used to identify people that belonged to the subject category "forensic science and legal medicine." This bibliometric information was derived from Elsevier's SCOPUS database containing eight million scientists with at least fiv...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02491-x
更新日期：2021-01-02 00:00:00
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journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050080
更新日期：1997-01-01 00:00:00
abstract：:In many countries, assessment of legal age, also known as age of majority, has become increasingly important over the years. In China, individuals older than 18 years of age have full capacity regarding civil conduct and can be tried as an adult for criminal charges. Therefore, from a legal point of view, it is crucia...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1907-4
更新日期：2018-11-01 00:00:00
abstract：:Various aspects concerning the practical application and forensic interpretation of data obtained by saliva drug testing and drug monitoring from the skin surface are discussed. Basic information on the composition of saliva and skin secretions and their particular transport mechanisms, as far as known, are given. For...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s004140050239
更新日期：1999-01-01 00:00:00
abstract：:The case of a man's death as a consequence of an accident with a mountain bike was examined. Despite only slight external injuries, a general examination revealed the existence of a rupture of the diaphragm which was the ultimate cause of death. The discussion reconstructs the way the accident occurred and briefly ana...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100237
更新日期：2001-12-01 00:00:00
abstract：:Using AGCU Y24 Plus PCR Amplification Kit, 32 Y short tandem repeat (STR) loci were analyzed in 355 unrelated male participants of Meizhou city in Guangdong Province of China. By analyzing 341 different haplotypes, it was found that haplotype diversity (HD) and discrimination capacity (DC) were 0.9995 and 0.9605, resp...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1845-1
更新日期：2019-03-01 00:00:00
