Abstract：
:Tumour necrosis factor-alpha (TNF-alpha)-converting enzyme (TACE) is a membrane-bound metalloprotease and disintegrin. It is produced by a number of host cells and is known to shed and release cell-bound cytokines, particularly members of the TNF family. No investigations into the regulation of this enzyme by autoantibodies have been reported. In this study, we tested the hypothesis that anti-Ro/SSA autoantibodies, purified from IgG fractions of patients with primary Sjögren's syndrome, are capable to regulate TACE expression and activation in human salivary gland epithelial cells (SGEC). We also evaluated the potential physiological and therapeutic consequences of TNF-alpha blocking by the biological agent adalimumab, the first fully human (100% human peptide sequences) therapeutic anti-TNF-alpha antibody, on post-translational regulation of TACE. Taken together, our results show a dose-dependent increase in TACE expression in anti-Ro/SSA Abs-treated SGEC, followed by internalization, pro-domain shedding and activation of TACE protein. Adalimumab treatment brought TACE expression to levels than those observed in untreated SGEC. These findings, showing the presence of autoantibodies-dependent mechanisms by which TACE levels are regulated in human SGECs, may have implications in the context of current investigations on the pathological role of autoantibodies.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Lisi S,Sisto Mdoi
10.1038/icb.2009.97subject
Has Abstractpub_date
2010-03-01 00:00:00pages
297-304issue
3eissn
0818-9641issn
1440-1711pii
icb200997journal_volume
88pub_type
杂志文章abstract：:Activin A, a member of the transforming growth factor-β superfamily, is a critical early mediator of acute inflammation. Activin A release coincides with the release of tumour necrosis factor-α (TNF-α) in models of lipopolysaccharide (LPS)-induced inflammation. The source of circulating activin A during acute inflamma...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2011.12
更新日期：2011-11-01 00:00:00
abstract：:Severe stress decreases the resistance of hosts exposed to microbial infections. As compared with two groups of control mice (normal mice, food-and-water-deprived mice [FWD mice]), restraint-stressed mice (RST mice) were shown to be greatly susceptible to intracerebral growth of Cryptococcus neoformans. The susceptibi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2006.01466.x
更新日期：2006-12-01 00:00:00
abstract：:The ability of a tunicate immunomodulatory protein, tunIL1, to interact with mammalian cells was investigated. TunIL1 is known to regulate inflammatory defence reactions in tunicates and to stimulate the proliferation of mammalian thymocytes. In the current study, tunIL1 was shown to enchance L929 fibroblast prolifera...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1996.3
更新日期：1996-02-01 00:00:00
abstract：:Within the immune system multiple mechanisms balance the need for efficient pathogen recognition and destruction with the prevention of tissue damage by excessive, inappropriate or even self-targeting (auto)immune reactions. This immune homeostasis is a tightly regulated system which fails during tumor development, of...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/imcb.12001
更新日期：2018-05-01 00:00:00
abstract：:Even though smallpox has been eradicated, the threat of accidental or intentional release has highlighted the fact there is little consensus about correlates of protective immunity or immunity against re-infection with the causative poxvirus, variola virus (VARV). As the existing vaccine for smallpox has unacceptable ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/sj.icb.7100118
更新日期：2008-01-01 00:00:00
abstract：:The Kunjin replicon was used to express a polytope that consisted of seven hepatitis C virus cytotoxic T lymphocyte epitopes and one influenza cytotoxic T lymphocyte epitope for vaccination studies. The self-replicating nature of, and expression from, the ribonucleic acid was confirmed in vitro. Initial vaccinations w...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.0818-9641.2002.01137.x
更新日期：2003-02-01 00:00:00
abstract：:Lethal giant larvae-1 (Lgl-1) is an evolutionary conserved protein that regulates cell polarity in diverse lineages; however, the role of Lgl-1 in the polarity and function of immune cells remains to be elucidated. To assess the role of Lgl-1 in T cells, we generated chimeric mice with a hematopoietic system deficient...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2015.82
更新日期：2016-03-01 00:00:00
abstract：:The NZB/W F1 (F1) mice develop severe disease that is similar to human systemic lupus erythematosus. By contrast, each parent strain, NZB or NZW, has limited autoimmunity, suggesting traits of both strains contribute to pathogenesis. Although many of the contributing genes have been identified, the contributing cellul...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12313
更新日期：2020-03-01 00:00:00
abstract：:Intravenous (i.v.) immunization of mice with recombinant vaccinia viruses stimulated the highest antibody and cytotoxic T lymphocyte (CTL) responses when i.v., intraperitoneal (i.p.), intranasal (i.n.), footpad (f.p.) and tail scarification (t.s.) routes were compared. Intraperitoneal immunization of mice resulted in ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1989.48
更新日期：1989-10-01 00:00:00
abstract：:Non-toxic agents that target intracellular signalling pathways in apoptosis may have potential therapeutic use in many diseases. One such agent is the transition metal Zn, a dietary cytoprotectant and anti-oxidant, which stimulates cell proliferation and suppresses apoptosis. Zn is maintained in discrete subcellular p...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1999.00825.x
更新日期：1999-06-01 00:00:00
abstract：:Thy-1 (CD90) expressed by mouse T cells is known to have signal transducing properties, but the ability of Thy-1 to enhance cytotoxic T lymphocyte (CTL) development is not well understood. Here we show that stimulation of mouse T cells with monoclonal antibodies (mAb) to CD3, CD28 and Thy-1 (clone G7), which were coim...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01342.x
更新日期：2005-08-01 00:00:00
abstract：:Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as bein...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1999.00877.x
更新日期：1999-12-01 00:00:00
abstract：:Mucosal addressin cell adhesion molecule (MAdCAM-1) is a key player in mediating the infiltration of leucocytes into chronically inflamed tissues. Five anti-MAdCAM-1 monoclonal antibodies (mAb), designated 17F5, 201F7, 314G8, 377D10 and 355G8, were generated by fusion of P3 x 63Ag8.653 myeloma cells with spleen cells ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.0818-9641.2004.01247.x
更新日期：2004-08-01 00:00:00
abstract：:CD8(+) T cells kill pancreatic β-cells in a cell-cell contact-dependent mechanism in the non-obese diabetic mouse. CD4(+) T lymphocytes are also able to kill pancreatic β-cells, but they do not directly contact β-cells and may use another cell type as the actual cytotoxic cell. Natural killer (NK) cells could have thi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2011.16
更新日期：2012-02-01 00:00:00
abstract：:This article attempts to assemble theoretical-teleological argument to explore possible answers to the question of why class I MHC molecules bind smaller peptides than class II MHC molecules and the associated question of why the size of peptides binding to class I molecules is approaching the limit of the self-non-se...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1995.15
更新日期：1995-02-01 00:00:00
abstract：:Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2003.01196.x
更新日期：2003-12-01 00:00:00
abstract：:Latex beads, 250 and 610 nm in diameter, and parapox virus isolated from ecthyma in sheep, were injected into intestinal loops containing either jejunal or ileal Peyer's patches (PP) of 3-4 week old calves. Uptake of latex and parapox virus was restricted to the ileal PP, 30-60 min after injection. The latex beads see...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.35
更新日期：1988-08-01 00:00:00
abstract：:The mucosal system is the first line of defense against many pathogens. It is continuously exposed to dietary and microbial antigens, and thus the host must maintain a homeostatic environment between commensal microbiota and pathogenic infections. Following infections and inflammatory events, a rapid innate immune res...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2012.13
更新日期：2012-05-01 00:00:00
abstract：:The molecular interactions between the T-cell receptor (TCR) and peptide-MHC (pMHC) have been elucidated in recent years. Nevertheless, the fact that binding of only slightly different ligands by a TCR, or ligation of the same pMHC at different developmental stages of the T cell, can have opposing consequences, contin...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2001.00994.x
更新日期：2001-04-01 00:00:00
abstract：:It has been described that polymorphonuclear neutrophils (PMNs) enhance the replication of CC-chemokine receptor 5/macrophage-tropic (R5) HIV in cultures of monocyte-derived macrophages (MDMs). In this study, the inhibitory effect of glycyrrhizin (GL) on R5 HIV replication influenced by PMNs was investigated in MDM cu...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2009.40
更新日期：2009-10-01 00:00:00
abstract：:Cells with a low density of parasite-specific antigens on their surface are postulated to be susceptible to a cell-mediated attack but not to effector mechanisms normally activated following the binding of specific antibody to the infected cell. It is further postulated that such infected cells normally induce a cell-...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1992.44
更新日期：1992-10-01 00:00:00
abstract：:In order to analyse Th2-type immune responses in sheep by the assay of interleukin (IL)-5 in biological fluids, the ovine IL-5 gene was cloned and expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus expression vector system. The recombinant product was purified as BAC-OV-IL-5 from the supernatant...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.1999.00831.x
更新日期：1999-08-01 00:00:00
abstract：:The mechanisms involved in sequential immunoglobulin G (IgG) class switching are still largely unknown. Sequential IG class switching is linked to higher levels of somatic hypermutation (SHM) in vivo, but it remains unclear if these are generated temporally during an immune response or upon activation in a secondary r...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2017.43
更新日期：2017-10-01 00:00:00
abstract：:Using 'knockin' mice to track and ablate dendritic cells (DCs) expressing notably the langerin (Cd207) gene, it has been possible to identify five DC subsets within the skin and to assess whether functional specialization exists among them. The present review summarizes recent information concerning the phenotype and ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2010.34
更新日期：2010-05-01 00:00:00
abstract：:Previous studies established that the high density (resting) natural killer (NK) cell subset (R-NK) of peripheral blood NK cells is unresponsive to interleukin-2 (IL-2) but can be induced to proliferate when cultured with gamma-irradiated malignant melanoma (MM-170) cells or mitomycin-C-treated activated T cells in th...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.9
更新日期：1993-04-01 00:00:00
abstract：:Natural killer (NK) cells are potent immune effector cells that can respond to infection and cancer, as well as allowing maternal adaptation to pregnancy. In response to malignant transformation or pathogenic invasion, NK cells can secrete cytokine and may be directly cytolytic, as well as exerting effects indirectly ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2010.78
更新日期：2011-02-01 00:00:00
abstract：:A hypothesis if formulated for the mode of action of the 'atopy gene': the gene(s) causing an increased tendency to mount an IgE immune response to trivial environmental antigens. It is postulated that some antigens are not efficiently transported to the lymphoid organs, the location of the 'eutopic' immune system; mi...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1996.28
更新日期：1996-04-01 00:00:00
abstract：:Lipid rafts are small laterally mobile microdomains that are highly enriched in lymphocyte signaling molecules. GM1 gangliosides are a common lipid raft component and have been shown to be important in many T-cell functions. The aggregation of specific GM1 lipid rafts can control many T-cell activation events, includi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2008.103
更新日期：2009-05-01 00:00:00
abstract：:The review articles in this special feature reflect the current status of knowledge in the field of exercise immunology, with a focus on how exercise affects the human immune system and the health implications for resistance to infections and neoplastic diseases. In an Olympic year, the emphasis of exercise immunology...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2000.t01-13-.x
更新日期：2000-10-01 00:00:00
abstract：:The secreted hexameric form of the dengue virus (DENV) non-structural protein 1 (NS1) has recently been shown to elicit inflammatory cytokine release and disrupt endothelial cell monolayer integrity. This suggests that circulating NS1 contributes to the vascular leak that plays a major role in the pathology of dengue ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2017.5
更新日期：2017-05-01 00:00:00
