Abstract：
:Mucosal addressin cell adhesion molecule (MAdCAM-1) is a key player in mediating the infiltration of leucocytes into chronically inflamed tissues. Five anti-MAdCAM-1 monoclonal antibodies (mAb), designated 17F5, 201F7, 314G8, 377D10 and 355G8, were generated by fusion of P3 x 63Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human MAdCAM-1-Fc. The latter four mAb recognize the ligand-binding first Ig domain, and block T -cell adhesion to MAdCAM-1. The non-blocking mAb 17F5 recognizes the mucin domain. Extensive analysis of a large panel of paraffin-embedded human tissues revealed that the 314G8 mAb detected MAdCAM-1 on venules in the spleen and small intestine. MAdCAM-1 was strongly expressed in the synovium of osteoarthritis patients, predominantly on the endothelial lining of blood vessels, but also within the vessel lumen. An ELISA, based on mAb 377D10 and 355G8, was developed to determine whether soluble MAdCAM-1 was present in body fluids, and to measure the levels present. The assay detected soluble MAdCAM-1 in the serum and urine of healthy donors, at levels similar to those of soluble forms of the related CAM, ICAM-1 and VCAM-1. The anti-MAdCAM-1 antibodies and assay developed here may be useful therapeutically in the treatment of inflammation in humans. Similarly, they may be useful diagnostically to monitor the presence and levels of MAdCAM-1.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Leung E,Lehnert KB,Kanwar JR,Yang Y,Mon Y,McNeil HP,Krissansen GWdoi
10.1111/j.0818-9641.2004.01247.xsubject
Has Abstractpub_date
2004-08-01 00:00:00pages
400-9issue
4eissn
0818-9641issn
1440-1711pii
ICB1247journal_volume
82pub_type
杂志文章abstract：:γδ T cells producing interleukin-17A (γδT17) are thought to develop spontaneously in the thymus and to be maintained in the periphery. Previous studies suggested a role for T-helper 17 (Th17) cells in the maintenance of γδT17 via the expression of transforming growth factor-β1 (TGFβ1). However, we have previously foun...
journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1994-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Immunology and cell biology
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pub_type： 杂志文章,评审
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更新日期：2005-12-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1989.16
更新日期：1989-04-01 00:00:00
