Abstract：
:The present paper describes a comparative analysis of light chains associated with primary and secondary IgM, as well as with secondary IgG antibodies to fluorescein, undertaken in order to explore the relationship between light chain somatic hypermutation and the isotype switch. The data reveal a disparity in the frequency of somatic hypermutation of secondary IgM heavy versus light chains. Among 20 secondary IgM light chains, a mutation frequency of 1/777 nucleotides was defined. In contrast, our previous analysis of the heavy chains of these molecules had identified a mutation frequency of 1/129. Among 17 IgG-derived light chains, obtained from animals killed at the same time point as those from which the secondary IgM antibodies were obtained, we measured a mutation frequency of 1/77. Finally, analysis of 20 light chains derived from primary IgM antibodies revealed a mutation frequency of only 1/1192 nucleotides. These data demonstrate that, prior to the class switch, light chain mutation occurs at a frequency considerably lower than that measured for the associated heavy chain gene. Six additional apparent mutations in the secondary IgM antibody 95B3 were all shared with a set of IgG antifluorescein antibodies belonging to the Vkappa 34 family. It is suggested that these light chains represent the products of a previously uncharacterized germ line gene.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
van der Keyl H,Gellad ZF,Owen JAdoi
10.1046/j.1440-1711.2000.00903.xsubject
Has Abstractpub_date
2000-06-01 00:00:00pages
224-37issue
3eissn
0818-9641issn
1440-1711pii
icb903journal_volume
78pub_type
杂志文章abstract：:Cancer immunosurveillance representing, till recently, the explanatory framework relating cancer and the immune system, does not convincingly explain tumor escape. At the beginning of the decade, a new theory emerged, namely the immunoediting theory, and it comprehensively defines the role of the immune system in carc...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1989-10-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2006-10-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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