Abstract：
:Pseudomonas aeruginosa ocular infection causes extensive corneal neovascularization. The purpose of the present study was to investigate the role of the angiogenic factors macrophage inflammatory protein-2 (MIP-2) and vascular endothelial growth factor (VEGF) in the regulation of corneal neovascularization during P. aeruginosa ocular infection. After administering anti-MIP-2 antibody or control antibody, mouse corneas were challenged with P. aeruginosa. The expression of MIP-2 and VEGF was detected using an ELISA from ocular homogenates. Corneal neovascularization was examined by histology. The cellular sources of MIP-2 and VEGF were identified by immunohistochemistry. In addition, protein expression of MIP-2 and VEGF in isolated corneas was measured to determine the ability of the cornea to produce these two mediators. Results showed that the expression of MIP-2 and VEGF was significantly (P < 0.05) elevated after bacterial infection, and high levels of these two mediators paralleled the extensive corneal neovascularization seen at later stages of the infection. Anti-MIP-2 antibody treatment resulted in a significant (P < 0.05) reduction in VEGF expression and in corneal neovascularization. Both corneal resident cells and infiltrating neutrophils had the ability to produce MIP-2 and VEGF after stimulation. The present study demonstrates that both MIP-2 and VEGF are important mediators in the regulation of corneal neovascularization caused by P. aeruginosa infection, and that MIP-2 regulates the production of VEGF.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Xue ML,Thakur A,Willcox Mdoi
10.1046/j.1440-1711.2002.01094.xsubject
Has Abstractpub_date
2002-08-01 00:00:00pages
323-7issue
4eissn
0818-9641issn
1440-1711pii
1094journal_volume
80pub_type
杂志文章abstract：:zeta (CD3-zeta) and gamma (Fc epsilon RI gamma) chains associate with CD16, the low affinity receptor for IgG (Fc gamma RIII) on human NK cells and are essential for the cell surface expression of CD16 and for CD16-mediated effector functions. This study has investigated whether, on NK cells, molecules other than CD16...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.13
更新日期：1994-02-01 00:00:00
abstract：:Ikaros, Aiolos and Helios encode zinc finger transcription factors that are important regulators of lymphoid development and differentiation. These proteins are involved in the control of gene expression and when associated with nuclear complexes, participate in nucleosome remodeling. Because differential splicing pro...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2003.01159.x
更新日期：2003-06-01 00:00:00
abstract：:Natural killer (NK) cells are potent immune effector cells that can respond to infection and cancer, as well as allowing maternal adaptation to pregnancy. In response to malignant transformation or pathogenic invasion, NK cells can secrete cytokine and may be directly cytolytic, as well as exerting effects indirectly ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2010.78
更新日期：2011-02-01 00:00:00
abstract：:Somatostatin inhibits in vitro lymphocyte proliferative responses from a variety of species including human, mouse and rat. The immunoinhibitory effects of somatostatin are thought to involve binding to specific cell surface somatostatin receptors on immunocompetent cells. This report describes an in vivo immunoinhibi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.13
更新日期：1993-04-01 00:00:00
abstract：:Genetic variants of interleukin 2 (IL-2) and its receptor are associated with murine and human susceptibility to Type 1 diabetes, yet the role of IL-2 in controlling pancreatic islet-reactive T cells is unknown. Here, we develop a model where IL-2 deficiency precipitates a breakdown of self-tolerance and progression t...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/sj.icb.7100049
更新日期：2007-06-01 00:00:00
abstract：:Severe stress decreases the resistance of hosts exposed to microbial infections. As compared with two groups of control mice (normal mice, food-and-water-deprived mice [FWD mice]), restraint-stressed mice (RST mice) were shown to be greatly susceptible to intracerebral growth of Cryptococcus neoformans. The susceptibi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2006.01466.x
更新日期：2006-12-01 00:00:00
abstract：:See also: Outstanding Observation by Wirasinha et al., Short Communication by Barnes & Cyster. ...
journal_title：Immunology and cell biology
pub_type： 评论,杂志文章
doi：10.1111/imcb.12027
更新日期：2018-04-01 00:00:00
abstract：:The multimammate rat Mastomys natalensis, when infected with the filarial parasite Acanthocheilonema viteae, develops amicrofilaraemia. Worm recovery and the duration and intensity of microfilaraemia were analysed and related to the humoral and cellular immune responses of the host by using an antibody-dependent cell-...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1990.3
更新日期：1990-02-01 00:00:00
abstract：:The NK gene complex (NKC) on mouse chromosome 6 encodes receptors that are expressed on NK cells, such as Ly49H, and is involved in regulating NK cell control of virus infections, such as murine cytomegalovirus (MCMV). In the present study, we investigated the level of allelic heterogeneity in NKC loci in populations ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01311.x
更新日期：2005-04-01 00:00:00
abstract：:Understanding how inhibitor of apoptosis proteins (IAPs) regulate apoptosis and necroptosis has been fast-forwarded by the use of Smac mimetics (SMs) to deplete or inhibit the IAPs, specifically cIAP1, cIAP2 and XIAP. The loss or inhibition of cIAP1, cIAP2 and XIAP causes the majority of cells to be sensitized to deat...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2016.118
更新日期：2017-02-01 00:00:00
abstract：:Productive immune responses to T-cell-dependent antigens ultimately generate two long-lived compartments: memory B cells and bone marrow-resident plasma cells, which both arise from within germinal centres. The ability of a B-cell clone to populate these effector compartments requires its descendents to outcompete tho...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/sj.icb.7100148
更新日期：2008-02-01 00:00:00
abstract：:Despite the potential for extensive diversity of gamma delta TCR, especially for delta-chains, expression of the gamma delta TCR repertoire in various mouse epithelial tissues is highly restricted. This implies that the recognition of antigen by gamma delta T cells may also be limited. To date, however, few studies ha...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1996.56
更新日期：1996-08-01 00:00:00
abstract：:Intravenous (i.v.) immunization of mice with recombinant vaccinia viruses stimulated the highest antibody and cytotoxic T lymphocyte (CTL) responses when i.v., intraperitoneal (i.p.), intranasal (i.n.), footpad (f.p.) and tail scarification (t.s.) routes were compared. Intraperitoneal immunization of mice resulted in ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1989.48
更新日期：1989-10-01 00:00:00
abstract：:T-cell repertoire is selected according to self peptide-MHC (major histocompatibility complex) complexes in the thymus. Although most peripheral T cells recognize specific pathogen-derived peptides complexed to self-MHC exclusively, some possess cross-reactivity to other self or foreign peptides presented by self-MHC ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2014.113
更新日期：2015-05-01 00:00:00
abstract：:Active Brugia pahangi microfilariae (Mf) were injected into naive male BALB/c mice intraperitoneally. Microfilaraemia was studied for 28 days; and Mf circulated in blood in optimum numbers from 3 to 14 days. Anti-Mf response was assessed by the rate of disappearance of Mf from blood as well as their absence from visce...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.43
更新日期：1988-10-01 00:00:00
abstract：:The signals that determine the survival/death of the thymic epithelial cells (TECs) component during embryonic development of the thymus are largely unknown. In this study, we combine different in vivo and in vitro experimental approaches to define the role played by the tyrosine kinase receptors EphB2 and EphB3 and t...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2012.59
更新日期：2013-02-01 00:00:00
abstract：:Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as bein...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1999.00877.x
更新日期：1999-12-01 00:00:00
abstract：:We recently demonstrated that 17beta-estradiol (E2) enhances killing of Leishmania mexicana in macrophages from both male and female DBA/2 mouse by increasing nitric oxide (NO) production. Here, we analyzed the effect of E2 on leishmanicidal activity and cytokine production by bone marrow-derived macrophages (BMDMs) f...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2008.39
更新日期：2008-08-01 00:00:00
abstract：:The present study demonstrates that SRBC can be opsonized with untreated human serum such that lysis by active complement components is minimal but sufficient opsonization occurs to permit high rates of complement-mediated phagocytosis. Phagocytosis of SRBC opsonized with 2% whole human serum by human monocyte-derived...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2001.01027.x
更新日期：2001-10-01 00:00:00
abstract：:Even though smallpox has been eradicated, the threat of accidental or intentional release has highlighted the fact there is little consensus about correlates of protective immunity or immunity against re-infection with the causative poxvirus, variola virus (VARV). As the existing vaccine for smallpox has unacceptable ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/sj.icb.7100118
更新日期：2008-01-01 00:00:00
abstract：:A murine monoclonal antibody has been used to characterise a 45,000 Da antigen that is associated with the surface membrane of merozoites of the human malaria parasite Plasmodium falciparum. The antigen is a glycoprotein and incorporates myristic acid. ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1987.48
更新日期：1987-10-01 00:00:00
abstract：:CD4 T cells play a key role in immunological memory. We have demonstrated that professional memory CD4 T cells reside and rest in the bone marrow (BM). However, the molecular mechanisms of their establishment in the BM and their maintenance remain unclear. We here show that memory CD4 T cells express high levels of CD...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2013.36
更新日期：2013-09-01 00:00:00
abstract：:Recombinant DNA techniques were used to clone, construct and express the bifunctional molecule FV/IL-2. The FV/IL-2 is a single-chain 37 kDa fusion protein expressed in Escherichia coli under control of the strong T7 bacteriophage promoter in the expression vector pT7-7-FV-IL-2. The fused gene fragment FV-IL-2 contain...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.42
更新日期：1994-08-01 00:00:00
abstract：:Depending on the microenvironment conditions, macrophages display phenotypic and functional heterogeneity. This study characterized the programmed cell death-ligand 2 (PD-L2)-expressing macrophage-like cells drained from surgical wound zones, and investigated their influence on helper T (Th) cell responses. Although a...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12310
更新日期：2020-02-01 00:00:00
abstract：:Within the immune system multiple mechanisms balance the need for efficient pathogen recognition and destruction with the prevention of tissue damage by excessive, inappropriate or even self-targeting (auto)immune reactions. This immune homeostasis is a tightly regulated system which fails during tumor development, of...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/imcb.12001
更新日期：2018-05-01 00:00:00
abstract：:S100A8 and S100A9 are myeloid cell-derived proteins that are elevated in several types of inflammatory lung disorders. Pro- and anti-inflammatory properties are reported and these proteins are proposed to activate TLR4. S100A8 and S100A9 can function separately, likely through distinct receptors but a systematic compa...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2017.2
更新日期：2017-05-01 00:00:00
abstract：:A general criterion is formulated to decide if the recycling of B cells exists in germinal centre (GC) reactions. The criterion is independent of the selection and affinity maturation process and based solely on total centroblast population arguments. An experimental test is proposed to verify whether the criterion is...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2002.01052.x
更新日期：2002-02-01 00:00:00
abstract：:The HECT-type E3 ligase Itch is increasingly being shown to have a vital role in immune regulation. Itch deficiency leads to deleterious inflammatory disorders both in mice and humans. By adding ubiquitin to the key signaling intermediates, Itch functions as a critical regulator of lymphocyte-cell activation, differen...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2014.118
更新日期：2015-05-01 00:00:00
abstract：:For the past 20 years footrot vaccines have evolved from simple bacterins to highly specific recombinant DNA (rDNA) fimbrial vaccines. The development of these vaccines has left a trail of discoveries, challenges and solutions; these processes continue as we move closer to understanding the requirements of a footrot v...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1993.53
更新日期：1993-10-01 00:00:00
abstract：:The 120-kDa protein product of the c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic protein that is especially abundant in the thymus, indicating an important role for Cbl in thymic signalling. c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain, a C3HC4 RING finger motif, multiple...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1998.00768.x
更新日期：1998-10-01 00:00:00
