Abstract:
:In order to analyse Th2-type immune responses in sheep by the assay of interleukin (IL)-5 in biological fluids, the ovine IL-5 gene was cloned and expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus expression vector system. The recombinant product was purified as BAC-OV-IL-5 from the supernatant fluid. The ovine IL-5 was biologically active in a bioassay using IL-5-dependent Baf cells, which have been used previously to specifically detect human IL-5. The specificity of Baf cells for ovine IL-5 was examined by two methods. First, Baf cells only proliferated in response to BAC-OV-IL-5 and did not respond to addition of recombinant ovine cytokines granulocyte-macrophage colony stimulating factor (GM-CSF), IL-1beta, IL-2, IL-3, IL-6, IL-8, stem cell factor (SCF) or IFN-gamma at doses from 0.01 to 1 microg/well. Second, the rat monoclonal antibody to murine IL-5, TRFK-5, neutralized murine, but not ovine, IL-5. However, rabbit antisera to BAC-OV-IL-5 neutralized murine and ovine recombinant IL-5 and abolished responses of Baf cells to IL-5 activity in supernatant fluids from mesenteric lymph node cells (MLNC) of parasitized sheep. The bioassay had a sensitivity to detect 8 ng in a 200 microL assay (40 ng/mL). Thus, the specificity of Baf cells to detect human IL-5 also extends to ovine IL-5 and therefore provides a method for monitoring the production of Th2 immune reactivity in sheep.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Bosward K,Emery DL,McWaters PW,Husband AJ,Bendixsen Tdoi
10.1046/j.1440-1711.1999.00831.xsubject
Has Abstractpub_date
1999-08-01 00:00:00pages
331-6issue
4eissn
0818-9641issn
1440-1711pii
icb831journal_volume
77pub_type
杂志文章abstract::This report summarizes recent advances on host-pathogen interactions, innate and adaptive responses to infection, as well as novel strategies for the control of infectious diseases. ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2012.40
更新日期:2012-09-01 00:00:00
abstract::Tumour necrosis factor-alpha (TNF-alpha)-converting enzyme (TACE) is a membrane-bound metalloprotease and disintegrin. It is produced by a number of host cells and is known to shed and release cell-bound cytokines, particularly members of the TNF family. No investigations into the regulation of this enzyme by autoanti...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2009.97
更新日期:2010-03-01 00:00:00
abstract::Elucidating pathways that regulate cytokine production in the context of autoimmune disease will likely lead to the development of novel therapeutics. Herein, we review data suggesting that microRNAs (miRs) represent one such level of regulatory activity, with particular emphasis on the pathogenesis of rheumatoid arth...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2011.114
更新日期:2012-03-01 00:00:00
abstract::The MS4A (membrane-spanning 4-domain family, subfamily A) family of proteins contains some well-known members including MS4A1 (CD20), MS4A2 (FcɛRIβ) and MS4A3 (HTm4). These three MS4A family members are expressed on the cell surface of specific leukocyte subsets and have been well characterized as having key roles in ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2015.48
更新日期:2016-01-01 00:00:00
abstract::The discovery of functional long noncoding RNAs (lncRNAs) coupled with the ever-increasing accessibility of genomic and transcriptomic technology has led to an explosion of functional and mechanistic investigation and discovery into what was once dismissed as junk DNA. Over the past decade, a significant number of lnc...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2015.2
更新日期:2015-03-01 00:00:00
abstract::PVG rats are resistant to actively induced experimental autoimmune encephalomyelitis (EAE) and this appears to be directly related to high and sustained systemic levels of reactive nitrogen intermediates(RNI) following sensitization for EAE when compared to the highly susceptible Lewis rat. An apparent cellular basis ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2002.01072.x
更新日期:2002-04-01 00:00:00
abstract::T-cell activation RhoGTPase-activating protein (TAGAP) is a GTPase-activating protein specific for RhoA that is exclusively expressed in activated T cells. Genome-wide association studies and metagenome SNPs analyses have indicated that TAGAP is associated with the pathogenesis of multiple autoimmune diseases, includi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2017.27
更新日期:2017-09-01 00:00:00
abstract::There has been a tremendous explosion in the area of DNA vaccine research over the last 4 years, particularly in relation to antiviral vaccines. This report discusses the development and application of this new technology with regard to parasitic infections. Progress has been made towards the development of a vaccine ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1997.58
更新日期:1997-08-01 00:00:00
abstract::For the past 20 years footrot vaccines have evolved from simple bacterins to highly specific recombinant DNA (rDNA) fimbrial vaccines. The development of these vaccines has left a trail of discoveries, challenges and solutions; these processes continue as we move closer to understanding the requirements of a footrot v...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1993.53
更新日期:1993-10-01 00:00:00
abstract::After positive selection, the newly differentiated single-positive (SP) thymocytes undergo negative selection to eliminate autoreactive T cells, functional maturation to acquire immunocompetence and egress capability. To investigate whether the intracellular reduction/oxidation (redox) balance has an important role on...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2013.57
更新日期:2013-11-01 00:00:00
abstract::FOXP3+ regulatory T (Treg) cells are indispensable for immune homeostasis, but their study in humans is complicated by heterogeneity within Treg, the difficulty in purifying Tregs using surface marker expression (e.g. CD25) and the transient expression of FOXP3 by activated effector cells. Here, we report that express...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2015.90
更新日期:2016-03-01 00:00:00
abstract::Leishmaniasis, a group of visceral and cutaneous diseases, is caused by parasites belonging to one genus comprising approximately 13 different species. Many methods including serological, biochemical and molecular biological techniques have been used by various workers to characterize these different species and isola...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.1998.00731.x
更新日期:1998-04-01 00:00:00
abstract::Th-cell differentiation is highly influenced by the local cytokine environment. Although cytokines such as IL-12 and IL-4 are known to polarize the Th-cell response towards Th1 or Th2, respectively, it is not known whether these cytokines instruct the developmental fate of uncommitted Th cells or select cells that hav...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2006.01425.x
更新日期:2006-04-01 00:00:00
abstract::Previous reports have documented that mice bearing plasmacytomas (PC) are suppressed in their ability to mount a primary antibody response, whereas cellular immunity appears to be normal. Studies presented here provide evidence that T cell responsiveness is also depressed in BALB/c mice bearing the Lieberman plasmacyt...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1991.3
更新日期:1991-02-01 00:00:00
abstract::Biallelic mutations in the human lipopolysaccharide responsive beige-like anchor (LRBA) gene lead to a primary immunodeficiency known as LRBA deficiency, characterized by a broad range of clinical manifestations including autoimmunity, organomegaly, hypogammaglobulinemia and recurrent infections. Considering the pheno...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2017.52
更新日期:2017-10-01 00:00:00
abstract::Peyer's patches (PP) are important inductive sites for the mucosal immune response. It is well known that lymphocytes that migrate into PP are mainly of T-cell lineage from thymus-derived cells (i.e. alphabetaTCR(high) cells). In this study, we further characterized the properties of PP lymphocytes in mice using a mou...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2005.01361.x
更新日期:2005-10-01 00:00:00
abstract::Mycobacterium tuberculosis uses numerous mechanisms to avoid elimination by the infected host. In this study, we investigated the possibility whether, similar to other pathogens, M. tuberculosis exploits natural CD4+ CD25+ T-regulatory cells (Treg) to suppress the effector function of responding host lymphocytes, thus...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2006.01460.x
更新日期:2006-10-01 00:00:00
abstract::Adoptive transfer of tumor-specific cytotoxic T cells is a promising advance in cancer therapy. Similarly, checkpoint inhibition has shown striking clinical results in some patients. Here we combine adoptive cell transfer with ablation of the checkpoint protein Src homology 2-domain-containing phosphatase 1 (SHP-1, Pt...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.45
更新日期:2016-09-01 00:00:00
abstract::Peroxidase-conjugated anti-surface immunoglobulin (sIg) was used quantitatively to monitor endocytosis of crosslinked sIg on murine B lymphocytes. The role of biochemical second messengers in the initiation of endocytosis was assessed by employing several inhibitors. A novel peroxidase detection system was used and te...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.1
更新日期:1993-02-01 00:00:00
abstract::Mice infected with Sarcocystis muris showed a significant reduction in plaque-forming cells (PFC) and delayed-type hypersensitivity (DHS) responses to an unrelated protein antigen, bovine gamma-globulin, when compared with uninfected controls. This immunosuppression was observed only when infection preceded immunizati...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1988.26
更新日期:1988-01-01 00:00:00
abstract::The Australian populations of rainbow trout (Onchorhynchus mykiss) and Atlantic salmon (Salmo salar) were found to have similar immunological responses to local strains of Vibrio anguillarum as those reported for the more genetically diverse populations of these fish and strains of V. anguillarum found in the Northern...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1992.51
更新日期:1992-12-01 00:00:00
abstract::The CD8 co-receptor engages peptide-major histocompatibility complex class I (pMHCI) molecules at a largely invariant site distinct from the T-cell receptor (TCR)-binding platform and enhances the sensitivity of antigen-driven activation to promote effective CD8+ T-cell immunity. A small increase in the strength of th...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.85
更新日期:2017-01-01 00:00:00
abstract::Early pregnancy factor (EPF), an extracellular chaperonin 10 homologue, has immunosuppressive and growth factor properties. In order to carry out more extensive studies on the in vivo characteristics of EPF, a recombinant form of the molecule has been prepared. Recombinant human EPF (rEPF) was expressed in Escherichia...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2000.00951.x
更新日期:2000-12-01 00:00:00
abstract::Cells with a low density of parasite-specific antigens on their surface are postulated to be susceptible to a cell-mediated attack but not to effector mechanisms normally activated following the binding of specific antibody to the infected cell. It is further postulated that such infected cells normally induce a cell-...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1992.44
更新日期:1992-10-01 00:00:00
abstract::The Kunjin replicon was used to express a polytope that consisted of seven hepatitis C virus cytotoxic T lymphocyte epitopes and one influenza cytotoxic T lymphocyte epitope for vaccination studies. The self-replicating nature of, and expression from, the ribonucleic acid was confirmed in vitro. Initial vaccinations w...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.0818-9641.2002.01137.x
更新日期:2003-02-01 00:00:00
abstract::In the developing forebrain, mounting evidence suggests that neural stem cell proliferation and differentiation is regulated by growth factors. In vitro in the presence of serum, stem cell proliferation is predominantly mediated by fibroblast growth factor-2 (FGF-2) whereas neuronal differentiation can be triggered by...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.1998.00762.x
更新日期:1998-10-01 00:00:00
abstract::T-cell repertoire is selected according to self peptide-MHC (major histocompatibility complex) complexes in the thymus. Although most peripheral T cells recognize specific pathogen-derived peptides complexed to self-MHC exclusively, some possess cross-reactivity to other self or foreign peptides presented by self-MHC ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2014.113
更新日期:2015-05-01 00:00:00
abstract::Three murine monoclonal antibodies (BC1, BC2 and BC3) were developed against human milk fat globule membrane (HMFGM). By immunoperoxidase staining, it was found that the antigenic determinants had a predominant distribution in breast cancer tissue. In addition, the antibodies reacted preferentially with mucin derived ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1989.29
更新日期:1989-06-01 00:00:00
abstract::Ikaros, Aiolos and Helios encode zinc finger transcription factors that are important regulators of lymphoid development and differentiation. These proteins are involved in the control of gene expression and when associated with nuclear complexes, participate in nucleosome remodeling. Because differential splicing pro...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.2003.01159.x
更新日期:2003-06-01 00:00:00
abstract::A feature common to many blood cell cancers is the uncoupling of normal proliferative and differentiative events, both of which are intimately linked in the cell's developmental programme. In some cancers, further differentiative events have been associated with oncogenic progression and, in other cancers, terminal di...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1995.32
更新日期:1995-06-01 00:00:00