Abstract：
:The Kunjin replicon was used to express a polytope that consisted of seven hepatitis C virus cytotoxic T lymphocyte epitopes and one influenza cytotoxic T lymphocyte epitope for vaccination studies. The self-replicating nature of, and expression from, the ribonucleic acid was confirmed in vitro. Initial vaccinations with one dose of Kun-Poly ribonucleic acid showed that an influenza-specific cytotoxic T lymphocyte response was elicited more efficiently by intradermal inoculation compared with intramuscular delivery. Two micrograms of ribonucleic acid delivered in the ear pinnae of mice was sufficient to elicit a detectable cytotoxic T lymphocyte response 10 days post-vaccination. Further vaccination studies showed that four of the seven hepatitis C virus cytotoxic T lymphocyte epitopes were able to elicit weak cytotoxic T lymphocyte responses whereas the influenza epitope was able to elicit strong, specific cytotoxic T lymphocyte responses following three doses of Kun-Poly ribonucleic acid. These studies vindicate the use of the Kunjin replicon as a vector to deliver encoded proteins for the development of cell-mediated immune responses.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Ward SM,Tindle RW,Khromykh AA,Gowans EJdoi
10.1046/j.0818-9641.2002.01137.xsubject
Has Abstractpub_date
2003-02-01 00:00:00pages
73-8issue
1eissn
0818-9641issn
1440-1711pii
1137journal_volume
81pub_type
杂志文章abstract：:The MS4A (membrane-spanning 4-domain family, subfamily A) family of proteins contains some well-known members including MS4A1 (CD20), MS4A2 (FcɛRIβ) and MS4A3 (HTm4). These three MS4A family members are expressed on the cell surface of specific leukocyte subsets and have been well characterized as having key roles in ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2015.48
更新日期：2016-01-01 00:00:00
abstract：:Natural suppressor (NS) cell line (Clone 59) was established from the bone marrow of adult C3H/Hej mice in the presence of WEHI-3 conditioned media. Clone 59 cells suppressed the generation of cytotoxic T lymphocytes from normal mouse spleen. This suppression was seen at a responder-to-suppressor cell ratio of 1000:1 ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1995.51
更新日期：1995-08-01 00:00:00
abstract：:Mast cells (MC) can undergo significant changes in number and phenotype; these alterations result in the differential expression of growth factors and cytokines. Kit ligand (KL; stem cell factor) is produced by mesenchymal cells, and in the liver by biliary epithelial cells. Recent studies suggest that KL, and its rec...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01368.x
更新日期：2005-12-01 00:00:00
abstract：:The 120-kDa protein product of the c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic protein that is especially abundant in the thymus, indicating an important role for Cbl in thymic signalling. c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain, a C3HC4 RING finger motif, multiple...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1998.00768.x
更新日期：1998-10-01 00:00:00
abstract：:Substance abuse is a growing world-wide problem. The big four drugs of abuse that might lend themselves to immunotherapy are nicotine, cocaine, morphine/heroin and methamphetamine. Tobacco abuse has a well-known enormous impact on major chronic cardiovascular and pulmonary diseases, while the last three, aside from th...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2009.17
更新日期：2009-05-01 00:00:00
abstract：:The ability to accurately quantitate and experimentally examine epigenetic modifications across the human genome has exploded in the past decade. This has given rise to a wealth of new information concerning the contributions of epigenetic regulatory networks to the pathogenesis of human disease. In particular, immuno...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2014.105
更新日期：2015-03-01 00:00:00
abstract：:Interleukin (IL)-15 overexpression in eosinophilic gastrointestinal disorders is reported, but IL-15's role in promoting eosinophilic gastroenteritis is largely unknown. Therefore, we generated enterocyte-overexpressed IL-15 transgenic mice using Fabpi promoter. The Fabpi-IL-15 (iIL-15) transgenic mice showed induced ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.1036
更新日期：2018-03-01 00:00:00
abstract：:The NK gene complex (NKC) on mouse chromosome 6 encodes receptors that are expressed on NK cells, such as Ly49H, and is involved in regulating NK cell control of virus infections, such as murine cytomegalovirus (MCMV). In the present study, we investigated the level of allelic heterogeneity in NKC loci in populations ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01311.x
更新日期：2005-04-01 00:00:00
abstract：:During a chronic infection such as tuberculosis, the pool of tissue dendritic cells (DC) must be renewed by recruitment of both circulating DC progenitors and monocytes (Mo). However, the microenvironment of the inflammatory site affects Mo differentiation. As DC are critical for initiating a Mycobacterium tuberculosi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2010.22
更新日期：2010-10-01 00:00:00
abstract：:Chronic HIV infection is associated with increased risk of cardiovascular disease (CVD), including in patients with virological suppression. Persistent innate immune activation may contribute to the development of CVD via activation of monocytes in these patients. We investigated whether changes in monocyte phenotype ...
journal_title：Immunology and cell biology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/icb.2013.84
更新日期：2014-02-01 00:00:00
abstract：:A clone of cells secreting an antibody to an epidermal antigen was generated from a patient with a blistering skin lesion. Although produced by fusion of human lymphocytes to a HAT-sensitive myeloma, this clone of cells did not have characteristics of a hybridoma. A true hybridoma was produced by fusion of this clone ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.30
更新日期：1988-01-01 00:00:00
abstract：:The goal of this study was to further characterize the interaction between an abundant urinary glycoprotein, Tamm-Horsfall protein, and complement 1q to determine the robustness of this reaction under different environmental conditions (particularly pH) and to begin to determine the specificity of this reaction. The i...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2002.01125.x
更新日期：2002-12-01 00:00:00
abstract：:Lymphocytes exchanged rapidly and extensively between mutually tolerant, allogeneic rats following surgical parabiosis. Tolerated allogeneic cells persisted for an extended period in parabiotic partners after separation. A rapid exchange of cells was also observed between parabiosed syngeneic pairs of normal and allog...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1987.29
更新日期：1987-06-01 00:00:00
abstract：:Interleukin (IL)-9-producing CD4(+) T cells are a novel subset of T helper (Th) cells that develops independently of the Th1, Th2, Th17 and regulatory T-cell lineages. Similar to the murine model, transforming growth factor (TGF)-beta and IL-4 directed human naive CD4(+) T cells to produce IL-9. Whereas IL-4 suppresse...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2010.53
更新日期：2010-08-01 00:00:00
abstract：:Mycobacterial disease remains a serious global health problem. Tuberculosis causes more than 2 million deaths a year, and leprosy is still a cause of severe disability in many parts of the world. As a result of the study of individuals with marked susceptibility to usually nonpathogenic mycobacteria, as well as case-c...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/j.1440-1711.2006.01420.x
更新日期：2006-04-01 00:00:00
abstract：:Adjuvants are components that when added to subunit antigen (Ag) vaccines boost their immunogenicity and thus immune efficacy. However, there are few adjuvants that are approved for clinical use resulting in a critical need for the development of safe and effective adjuvants for use in both prophylactic and therapeuti...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2009.21
更新日期：2009-07-01 00:00:00
abstract：:The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and b...
journal_title：Immunology and cell biology
pub_type： 临床试验,杂志文章
doi：10.1038/icb.2014.23
更新日期：2014-07-01 00:00:00
abstract：:There are estimated to be 350 million chronic carriers of hepatitis B infection worldwide. Patients with chronic hepatitis B are at risk of liver cirrhosis with associated mortality because of hepatocellular carcinoma and other complications. An important goal, therefore, is the development of an effective therapeutic...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.0818-9641.2004.01278.x
更新日期：2004-10-01 00:00:00
abstract：:The effects of endotoxin treatment on host metabolism in tumour-bearing rats were investigated. Metabolism in control rats (non-tumour-bearing) was slightly altered by endotoxin treatment, whereas in tumour-bearing rats a number of biochemical parameters that were initially perturbed by the presence of the tumour had ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1992.1
更新日期：1992-02-01 00:00:00
abstract：:Depending on the microenvironment conditions, macrophages display phenotypic and functional heterogeneity. This study characterized the programmed cell death-ligand 2 (PD-L2)-expressing macrophage-like cells drained from surgical wound zones, and investigated their influence on helper T (Th) cell responses. Although a...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12310
更新日期：2020-02-01 00:00:00
abstract：:Numerous studies have associated carriage of HLA-DRB1*1501, DQA1*0102 and DQB1*0602 (DR15, DQ6) with dominant resistance to type 1 diabetes and have concluded that one or more of the component HLA class II molecules mediate this effect. Mechanisms for MHC class II-mediated resistance to diabetes have been proposed fro...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2001.01043.x
更新日期：2001-12-01 00:00:00
abstract：:One of the major limitations for cancer immunotherapy is related to the frequent existence of an intra-tumoral immunosuppressive environment, to which STAT3 (Signal transducer and activator of transcription-3) activation in tumor and dendritic cells (DCs) are believed to contribute. In this study, we tested the hypoth...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2008.27
更新日期：2008-08-01 00:00:00
abstract：:Infections caused by group A streptococcus (GAS) represent a public health problem in both developing and developed countries. The current available methods of prevention are either inadequate or ineffective, which is highlighted by the resurgence in invasive GAS infections over the past two decades. The management of...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2009.29
更新日期：2009-07-01 00:00:00
abstract：:The effect of serum on LPS-induced activation of a murine macrophage-like cell line, WEHI-3, was examined. Foetal calf serum strongly inhibited the production of nitric oxide (NO) and TNF-alpha by LPS-stimulated WEHI-3 cells, while it enhanced the production of both by other macrophage-like cell lines, J774.1 and BAM3...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.1999.00807.x
更新日期：1999-04-01 00:00:00
abstract：:The iscom is a delivery system, designed for both parenteral and mucosal modes of administration, for both antigens and adjuvants, components which are interchangeable. By the parenteral route a prominent systemic Th1 type of response is evoked, but the mucosal immunoglobulin A (IgA) response was insignificant. Intran...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1998.00756.x
更新日期：1998-08-01 00:00:00
abstract：:A derivative of ammonia caramel colour (AC) is known to induce a selective lymphopenia in rats. Accordingly, the haematological effects were studied in mice of oral administration in drinking water of 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI), the component of AC responsible for lymphopenia. Initially five groups...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.51
更新日期：1988-10-01 00:00:00
abstract：:NK cell activity has been detected in the early murine decidua, and hypothesized to be mediated by granulated metrial gland (GMG) cells. The possibility that natural cytotoxic (NC) cells are also present in the decidua has not been investigated. In this study mAb to NK cells (anti-NK-1.1) and NC cells (anti-NC-1.1) we...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.23
更新日期：1994-04-01 00:00:00
abstract：:Multiple levels of regulation of collagenase (matrix metalloproteinase 1; MMP-1), have been demonstrated in a clonal rat epithelial cell line (A5P/B10). Secreted enzyme could not be demonstrated in culture medium from A5P/B10 cells but, using antibodies specific for collagenase, the enzyme was detected within the cyto...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.4
更新日期：1993-02-01 00:00:00
abstract：:This paper reports the production and characterization of three monoclonal antibodies (mAb) recognizing ovine vascular cell adhesion molecule-1 (VCAM-1). The mAb were raised against sheep umbilical vein endothelial cells (ShUVEC) and flow cytometric analysis demonstrated that one mAb, QE4G9, was cross-reactive with hu...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.85
更新日期：1997-12-01 00:00:00
abstract：:The evolutionarily conserved catabolic process of autophagy involves the degradation of cytoplasmic components through lysosomal enzymes. Basal levels of autophagy maintain cellular homeostasis and under stress conditions high levels of autophagy are induced. It is often under such stress conditions that high levels o...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2014.85
更新日期：2015-01-01 00:00:00
