Abstract:
:This paper reports the production and characterization of three monoclonal antibodies (mAb) recognizing ovine vascular cell adhesion molecule-1 (VCAM-1). The mAb were raised against sheep umbilical vein endothelial cells (ShUVEC) and flow cytometric analysis demonstrated that one mAb, QE4G9, was cross-reactive with human VCAM-1 expressed on Chinese hamster ovary cell transfectants. Protein modulation studies on ShUVEC and immunoperoxidase staining of inflamed renal tissue further indicated the reactivity of the other two ovine mAb, QE1F3 and QE2G4, with ovine VCAM-1. The flow cytometric profile of the three mAb on stimulated ShUVEC was identical to that observed with the cross-reactive anti-human VCAM- mAb, HAE2-1. Peak expression occurred between 6-12 h after stimulation, followed by a slight decrease to a plateau extending beyond 48 h. In functional assays, all mAb inhibited adhesion of PMA-activated sheep PBMC to stimulated ShUVEC. In addition, QE4G9 inhibited proliferation of sheep PBMC in the mixed lymphocyte-endothelial cell reaction (MLER) by 56%. The results demonstrate that the three anti-ovine VCAM-1 mAb recognize functional epitopes on sheep vascular endothelial cells. These mAb will be valuable tools in the investigation of VCAM-1 expression in various pathophysiological conditions using sheep models, and in the study of VCAM-1-mediated leucocyte-endothelial cell interactions, both in vitro and in vivo.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Grooby WL,Krishnan R,Russ GRdoi
10.1038/icb.1997.85subject
Has Abstractpub_date
1997-12-01 00:00:00pages
546-53issue
6eissn
0818-9641issn
1440-1711journal_volume
75pub_type
杂志文章abstract::A derivative of ammonia caramel colour (AC) is known to induce a selective lymphopenia in rats. Accordingly, the haematological effects were studied in mice of oral administration in drinking water of 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI), the component of AC responsible for lymphopenia. Initially five groups...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1988.51
更新日期:1988-10-01 00:00:00
abstract::Infection of mice with the flaviviruses West Nile virus (WNV) and Murray Valley encephalitis (MVE) induces cytolytic T-cell responses which are highly cross-reactive on target cells infected with heterologous flaviviruses. Of C57BL/6 mice infected with low doses (10(2)-10(6) PFU) of either virus, 30-40% develop enceph...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.0818-9641.2004.01227.x
更新日期:2004-04-01 00:00:00
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journal_title:Immunology and cell biology
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doi:10.1111/imcb.12001
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journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1996.77
更新日期:1996-10-01 00:00:00
abstract::Antigen presentation by enterocytes isolated from the rat small bowel was studied by using T cell proliferation, and immunoregulatory function in an antigen-driven culture system, as indicator systems. Lymph node T cells obtained from rats immunized with ovalbumin (OA) failed to divide when cultured for 4 days in the ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1990.52
更新日期:1990-12-01 00:00:00
abstract::Human regulatory T cells (Tregs) expressing the transcription factor FoxP3 play indispensable roles for the maintenance of immunological self-tolerance and immune homeostasis. In this review, we discuss immunological characteristics of human FoxP3(+) Tregs and to what extent they are similar to or different from the m...
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journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
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更新日期:1999-06-01 00:00:00
abstract::Construction of libraries of artificial antibodies has been reported by several groups of investigators. Various forms of antibody fused to surface protein, cpII, are expressed on the surface of filamentous phage. Since phages that encode desired antibodies can be easily grown in Escherichia coli after selection with ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1997.33
更新日期:1997-04-01 00:00:00
abstract::Fundamentally new approaches are required for the development of vaccines to pre-empt and protect against emerging and pandemic influenzas. Current strategies involve post-emergent homotypic vaccines that are modelled upon select circulating 'seasonal' influenzas, but cannot induce cross-strain protection against newl...
journal_title:Immunology and cell biology
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doi:10.1038/icb.2011.38
更新日期:2012-02-01 00:00:00
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journal_title:Immunology and cell biology
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journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.89
更新日期:2017-02-01 00:00:00
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journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/sj.icb.7100006
更新日期:2007-01-01 00:00:00
abstract::The broadest definition of dendritic cells (DCs) is white blood cells that can take up antigen, process it and then present antigen-derived peptides to activate cognate naive T cells. Although this definition is by no means perfect, it is nevertheless now textbook. The source of frustration more recently has focused o...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2008.28
更新日期:2008-07-01 00:00:00
abstract::Lymphocytes exchanged rapidly and extensively between mutually tolerant, allogeneic rats following surgical parabiosis. Tolerated allogeneic cells persisted for an extended period in parabiotic partners after separation. A rapid exchange of cells was also observed between parabiosed syngeneic pairs of normal and allog...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1987.29
更新日期:1987-06-01 00:00:00
abstract::The surface phenotype of T cells reflects both their relative maturity and their activation state. To determine the pattern of surface markers characteristic of activated T cells, purified mature T cells were stimulated in vitro for periods of 0.5-5 days with Concanavalin-A (Con-A) or phorbol myristic acetate (PMA) an...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1988.39
更新日期:1988-08-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is a common virus that causes lower respiratory infections across a wide range of ages. A licensed RSV vaccine is not available because vaccination with formalin-inactivated RSV (FI-RSV) and the subsequent RSV infection cause not only insufficient induction of neutralizing antibodies ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2017.61
更新日期:2017-11-01 00:00:00
abstract::See also: Outstanding Observation by Wirasinha et al., Short Communication by Barnes & Cyster. ...
journal_title:Immunology and cell biology
pub_type: 评论,杂志文章
doi:10.1111/imcb.12027
更新日期:2018-04-01 00:00:00
abstract::Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.2003.01196.x
更新日期:2003-12-01 00:00:00
abstract::PVG rats are resistant to actively induced experimental autoimmune encephalomyelitis (EAE) and this appears to be directly related to high and sustained systemic levels of reactive nitrogen intermediates(RNI) following sensitization for EAE when compared to the highly susceptible Lewis rat. An apparent cellular basis ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2002.01072.x
更新日期:2002-04-01 00:00:00
abstract::Somatostatin inhibits in vitro lymphocyte proliferative responses from a variety of species including human, mouse and rat. The immunoinhibitory effects of somatostatin are thought to involve binding to specific cell surface somatostatin receptors on immunocompetent cells. This report describes an in vivo immunoinhibi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.13
更新日期:1993-04-01 00:00:00
abstract::The effects of endotoxin treatment on host metabolism in tumour-bearing rats were investigated. Metabolism in control rats (non-tumour-bearing) was slightly altered by endotoxin treatment, whereas in tumour-bearing rats a number of biochemical parameters that were initially perturbed by the presence of the tumour had ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1992.1
更新日期:1992-02-01 00:00:00
abstract::Over the past few years a great deal of research has examined how T cell-dependent immune responses are initiated and subsequently regulated. Ligation of the TCR with an antigenic peptide bound to an MHC protein on a professional APC provides the crucial antigen-specific stimulus required for T cell activation. Intera...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.1999.00795.x
更新日期:1999-02-01 00:00:00
abstract::Understanding how inhibitor of apoptosis proteins (IAPs) regulate apoptosis and necroptosis has been fast-forwarded by the use of Smac mimetics (SMs) to deplete or inhibit the IAPs, specifically cIAP1, cIAP2 and XIAP. The loss or inhibition of cIAP1, cIAP2 and XIAP causes the majority of cells to be sensitized to deat...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2016.118
更新日期:2017-02-01 00:00:00
abstract::In order to analyse Th2-type immune responses in sheep by the assay of interleukin (IL)-5 in biological fluids, the ovine IL-5 gene was cloned and expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus expression vector system. The recombinant product was purified as BAC-OV-IL-5 from the supernatant...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.1999.00831.x
更新日期:1999-08-01 00:00:00
abstract::NK cell activity has been detected in the early murine decidua, and hypothesized to be mediated by granulated metrial gland (GMG) cells. The possibility that natural cytotoxic (NC) cells are also present in the decidua has not been investigated. In this study mAb to NK cells (anti-NK-1.1) and NC cells (anti-NC-1.1) we...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1994.23
更新日期:1994-04-01 00:00:00
abstract::Two related folate antagonists aminopterin (AMN) and methotrexate (MTX) were used to produce drug-antibody conjugates and their tumouricidal effects compared in vitro and in vivo. Active ester derivatives were produced with the use of N-hydroxysuccinimide (NHS) and covalently coupled to monoclonal antibodies (MoAb) re...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1987.57
更新日期:1987-12-01 00:00:00
abstract::The cellular effects of biodiesel emissions particulate matter (BDEP) and petroleum diesel emissions particulate matter (PDEP) were compared using a human airway cell line, A549. At concentrations of 25 microg/ml, diesel particulate matter induced the formation of multinucleate cells. In cells treated with a mixture o...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100109
更新日期:2007-11-01 00:00:00
abstract::The present paper describes a comparative analysis of light chains associated with primary and secondary IgM, as well as with secondary IgG antibodies to fluorescein, undertaken in order to explore the relationship between light chain somatic hypermutation and the isotype switch. The data reveal a disparity in the fre...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2000.00903.x
更新日期:2000-06-01 00:00:00
abstract::Asthma is a common and complex inflammatory disease of the airways that remains incurable. Current forms of therapy are long term and may exhibit associated side-effect problems. Major participants in the development of an asthma phenotype include the triggering stimuli such as the allergens themselves, cells such as ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.2001.00987.x
更新日期:2001-04-01 00:00:00
abstract::Activin A, a member of the transforming growth factor-β superfamily, is a critical early mediator of acute inflammation. Activin A release coincides with the release of tumour necrosis factor-α (TNF-α) in models of lipopolysaccharide (LPS)-induced inflammation. The source of circulating activin A during acute inflamma...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2011.12
更新日期:2011-11-01 00:00:00