Abstract:
:Thy-1 (CD90) expressed by mouse T cells is known to have signal transducing properties, but the ability of Thy-1 to enhance cytotoxic T lymphocyte (CTL) development is not well understood. Here we show that stimulation of mouse T cells with monoclonal antibodies (mAb) to CD3, CD28 and Thy-1 (clone G7), which were coimmobilized on polystyrene microbeads, resulted in a greater proliferative response than stimulation with only anti-CD3 and anti-CD28 mAb, indicating that Thy-1 cross-linking enhanced T cell receptor/CD28-driven T cell activation. Consistent with this finding, Thy-1 blockade with a soluble nonactivating anti-Thy-1 mAb (clone 30-H12) inhibited anti-CD3-induced proliferation of CD4+ and CD8+ T cells, and the induction of cytotoxic effector cells in a dose-dependent fashion. Interleukin-2 synthesis and CD25 expression were also impaired by Thy-1 blockade. The inhibitory effect involved a defect at or before the level of protein kinase C activation because the addition of phorbol ester ablated the anti-Thy-1-mediated inhibition of anti-CD3-induced T cell activation. The CTL that were induced in the presence of blocking anti-Thy-1 mAb adhered to target cells but showed reduced expression of granzyme B and perforin. In contrast, Fas ligand expression and function was not affected by Thy-1 blockade. We conclude that Thy-1 signalling promotes the in vitro generation of CTL that kill in a granule-dependent fashion.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Haeryfar SM,Conrad DM,Musgrave B,Hoskin DWdoi
10.1111/j.1440-1711.2005.01342.xsubject
Has Abstractpub_date
2005-08-01 00:00:00pages
352-63issue
4eissn
0818-9641issn
1440-1711pii
ICB1342journal_volume
83pub_type
杂志文章abstract::The role of the immunoproteasome is perceived as confined to adaptive immune responses given its ability to produce peptides ideal for MHC Class-I binding. Here, we demonstrate that the immunoproteasome subunit, LMP2, has functions beyond its immunomodulatory role. Using LMP2-deficient mice, we demonstrate that LMP2 i...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12163
更新日期:2018-10-01 00:00:00
abstract::Previous studies established that the high density (resting) natural killer (NK) cell subset (R-NK) of peripheral blood NK cells is unresponsive to interleukin-2 (IL-2) but can be induced to proliferate when cultured with gamma-irradiated malignant melanoma (MM-170) cells or mitomycin-C-treated activated T cells in th...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.9
更新日期:1993-04-01 00:00:00
abstract::Dendritic cells (DCs) orchestrate the innate and adaptive immune systems to induce tolerance and immunity. DC plasticity and subsets are prominent determinants in the regulation of immune responses. Our recent studies suggest that humoral and cellular immunity is regulated by different myeloid DC subsets with distinct...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2010.28
更新日期:2010-05-01 00:00:00
abstract::The alpha-gal epitope (Galalpha1-3Galbeta1-(3)4GlcNAc-R) is abundantly synthesized on glycolipids and glycoproteins of non-primate mammals and New World monkeys by the glycosylation enzyme alpha1,3galactosyltransferase (alpha1,3GT). In humans, apes and Old World monkeys, this epitope is absent because the alpha1,3GT g...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1111/j.1440-1711.2005.01366.x
更新日期:2005-12-01 00:00:00
abstract::Hereditary angioedema as a result of deficiency of the C1 inhibitor (HAE-C1INH; MIM# 106100) is a rare autosomal disorder and affected individuals are generally heterozygous for dominant negative variants in the SERPING1 gene. Homozygosity for SERPING1 pathogenic variants was long considered to be embryonically lethal...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12362
更新日期:2020-09-01 00:00:00
abstract::Hepatocyte growth factor (HGF) and its receptor c-Met signaling have been implicated in regulating various types of cells including epithelial cells. We have previously reported that c-Met is expressed by thymic epithelial cells (TECs), and that in vivo administration of hybrid cytokines containing IL-7 and the beta- ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.1026
更新日期:2018-02-01 00:00:00
abstract::Substance abuse is a growing world-wide problem. The big four drugs of abuse that might lend themselves to immunotherapy are nicotine, cocaine, morphine/heroin and methamphetamine. Tobacco abuse has a well-known enormous impact on major chronic cardiovascular and pulmonary diseases, while the last three, aside from th...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2009.17
更新日期:2009-05-01 00:00:00
abstract::The glycoprotein processing inhibitor castanospermine (CS) and the monosaccharide mannose-6-phosphate (M6P), as well as some sulfated polysaccharides (SPS), have been shown to inhibit inflammation in rat models of experimental autoimmune encephalomyelitis and adjuvant-induced arthritis. Here, the anti-inflammatory eff...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1994.55
更新日期:1994-10-01 00:00:00
abstract::Deoxyspergualin (DOSP) is a new immunosuppressive agent which probably inhibits various functions of monocytes, B cells and T cells. We examined the effects of deoxyspergualin on cellular cytotoxicity, including cytotoxic T lymphocyte (CTL) mediated killer, natural killer (NK) cell and lymphokine activated killer (LAK...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1991.26
更新日期:1991-06-01 00:00:00
abstract::Th-cell development has been suggested to include selective mechanisms in which certain cytokines select either Th1 or Th2 cells to proliferate and grow. The selective theory is based on the observation that Th2 cells secrete IL-4, a cytokine that promotes Th2 development, whereas Th1 cells secrete interferon-gamma (I...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100023
更新日期:2007-04-01 00:00:00
abstract::Granzyme B (GrB) plays a well-established intracellular role in cytotoxic lymphocyte (CL)-mediated killing of abnormal cells; however, emerging evidence suggests that it participates in extracellular matrix remodeling and target cell destruction through anoikis. As these processes require the release of GrB from the C...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2008.98
更新日期:2009-03-01 00:00:00
abstract::Sustained-release vaccine delivery systems may enhance the immunogenicity of subunit vaccines and reduce the need for multiple vaccinations. The aim of this study was to develop a thermoresponsive hydrogel using poloxamer 407-chitosan (CP) grafted copolymer as a delivery system for single-shot sustained-release vaccin...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12031
更新日期:2018-07-01 00:00:00
abstract::CD8+ T lymphocytes are key effectors in the control of viral diseases and some tumours. In general, the majority of CD8+ T cells recognize a few immunodominant epitopes, but in some circumstances, subdominant specificities may be more relevant as targets for vaccines or immunotherapy. Epstein-Barr virus (EBV)-associat...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100083
更新日期:2007-10-01 00:00:00
abstract::Tumour cell heterogeneity is probably a principal cause of treatment failure and represents a formidable barrier for effective antibody-targeted chemotherapy. Idarubicin (Ida), a more potent and less cardiotoxic analogue of daunomycin, has been demonstrated to specifically target and eradicate homogeneous, cloned, mur...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.19
更新日期:1993-06-01 00:00:00
abstract::Biallelic mutations in the human lipopolysaccharide responsive beige-like anchor (LRBA) gene lead to a primary immunodeficiency known as LRBA deficiency, characterized by a broad range of clinical manifestations including autoimmunity, organomegaly, hypogammaglobulinemia and recurrent infections. Considering the pheno...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2017.52
更新日期:2017-10-01 00:00:00
abstract::Interleukin-4 (IL-4) has previously been implicated in a protective response to Staphylococcus aureus corneal infection. Consequently, the specific role of IL-4 during S. aureus corneal infection was investigated using IL-4 gene knockout mice. The eyes of IL-4-/- mice and wild-type mice were challenged topically with ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100043
更新日期:2007-06-01 00:00:00
abstract::Even though smallpox has been eradicated, the threat of accidental or intentional release has highlighted the fact there is little consensus about correlates of protective immunity or immunity against re-infection with the causative poxvirus, variola virus (VARV). As the existing vaccine for smallpox has unacceptable ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/sj.icb.7100118
更新日期:2008-01-01 00:00:00
abstract::Peroxidase-conjugated anti-surface immunoglobulin (sIg) was used quantitatively to monitor endocytosis of crosslinked sIg on murine B lymphocytes. The role of biochemical second messengers in the initiation of endocytosis was assessed by employing several inhibitors. A novel peroxidase detection system was used and te...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.1
更新日期:1993-02-01 00:00:00
abstract::For the past 20 years footrot vaccines have evolved from simple bacterins to highly specific recombinant DNA (rDNA) fimbrial vaccines. The development of these vaccines has left a trail of discoveries, challenges and solutions; these processes continue as we move closer to understanding the requirements of a footrot v...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1993.53
更新日期:1993-10-01 00:00:00
abstract::Acute rejection is the major determinant for the long-term survival of donor liver after liver transplantation (LT). The aim of this study was to examine the therapeutic potential of interleukin (IL)-10-FasL-overexpressing immature dendritic cells (imDCs) to induce local immunosuppression in liver grafts. imDCs derive...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12252
更新日期:2019-09-01 00:00:00
abstract::The relationship between interleukin-1 (IL-1) and interleukin-2 (IL-2) production and immunophenotype marker profiles was studied in a panel of 29 leukaemia and human T cell lymphotropic virus-1 (HTLV-1)-transformed T cell lines. Culture supernatants from six of the 29 T cell lines tested increased IL-2 production by ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.56
更新日期:1993-12-01 00:00:00
abstract::The 120-kDa protein product of the c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic protein that is especially abundant in the thymus, indicating an important role for Cbl in thymic signalling. c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain, a C3HC4 RING finger motif, multiple...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.1998.00768.x
更新日期:1998-10-01 00:00:00
abstract::Lymphocyte activation was examined by interleukin-2 (IL-2) receptor expression on peripheral blood mononuclear cells from coeliac and control subjects. Purified T cells were incubated with gluten fraction 111 (a known toxic peptide for coeliac subjects), soyabean hydrolysate (an unrelated hydrolysed food antigen), and...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1990.22
更新日期:1990-06-01 00:00:00
abstract::In the last lustrum single-cell techniques such as single-cell quantitative PCR, RNA and DNA sequencing, and the state-of-the-art cytometry by time of flight (CyTOF) mass cytometer have allowed a detailed analysis of the sub-composition of different organs from the bone marrow hematopoietic compartment to the brain. T...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2015.106
更新日期:2016-03-01 00:00:00
abstract::There is an urgent need for new, powerful adjuvants suitable for use with sub-unit and peptide vaccines in humans. We have measured the humoral immune response in BALB/c mice to vaccine formulations using recombinant HBsAg antigens, and gamma inulin and alum adjuvants. Using Merck, Sharpe & Dohme (MSD) HBsAg at 10 mic...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1990.15
更新日期:1990-04-01 00:00:00
abstract::The NZB/W F1 (F1) mice develop severe disease that is similar to human systemic lupus erythematosus. By contrast, each parent strain, NZB or NZW, has limited autoimmunity, suggesting traits of both strains contribute to pathogenesis. Although many of the contributing genes have been identified, the contributing cellul...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12313
更新日期:2020-03-01 00:00:00
abstract::Mucosal mast cells (MMCs) have an important role in allergic inflammation, and effective antagonists are required for their regulation. To discover a possible mechanism of controlling the activation of MMCs, we investigated the expression and function of syntaxin4, one of the soluble membrane N-ethylmaleimide-sensitiv...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2011.41
更新日期:2012-03-01 00:00:00
abstract::Herpesviruses are an ancient, ubiquitous family of DNA viruses, most of which share a lifestyle of latently or persistently infecting a young host, and spreading to infect a new host a generation later. Most herpesviruses interfere with antigen presentation via the MHC class I-restricted pathway of antigen presentatio...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1996.85
更新日期:1996-12-01 00:00:00
abstract::Pseudomonas aeruginosa ocular infection causes extensive corneal neovascularization. The purpose of the present study was to investigate the role of the angiogenic factors macrophage inflammatory protein-2 (MIP-2) and vascular endothelial growth factor (VEGF) in the regulation of corneal neovascularization during P. a...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2002.01094.x
更新日期:2002-08-01 00:00:00
abstract::Heterogeneity of T cells is a hallmark of a successful adaptive immune response, harnessing the vast diversity of antigen-specific T cells into a coordinated evolution of effector and memory outcomes. The T cell receptor (TCR) repertoire is highly diverse to account for the highly heterogeneous antigenic world. During...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.16
更新日期:2016-07-01 00:00:00