Abstract：
:Granzyme B (GrB) plays a well-established intracellular role in cytotoxic lymphocyte (CL)-mediated killing of abnormal cells; however, emerging evidence suggests that it participates in extracellular matrix remodeling and target cell destruction through anoikis. As these processes require the release of GrB from the CL into the extracellular environment, we examined the secretion of GrB from natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). We found that a proportion of GrB is constitutively secreted by both CTLs and NK cells in the absence of target cell engagement. In NK cells, the protease is primarily released in an active form through secretory granules. By contrast, T lymphocytes primarily secrete inactive GrB zymogen, bypassing the granules. The release of GrB through two routes from unconjugated CLs suggests that it functions outside the cell and may contribute to pathology in cases of immune dysregulation, such as familial hemophagocytic lymphohistiocytosis (FHL). Our findings also predict the existence of an extracellular activator of GrB.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Prakash MD,Bird CH,Bird PIdoi
10.1038/icb.2008.98subject
Has Abstractpub_date
2009-03-01 00:00:00pages
249-54issue
3eissn
0818-9641issn
1440-1711pii
icb200898journal_volume
87pub_type
杂志文章abstract：:zeta (CD3-zeta) and gamma (Fc epsilon RI gamma) chains associate with CD16, the low affinity receptor for IgG (Fc gamma RIII) on human NK cells and are essential for the cell surface expression of CD16 and for CD16-mediated effector functions. This study has investigated whether, on NK cells, molecules other than CD16...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.13
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1993-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1996-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：1996-04-01 00:00:00
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更新日期：2013-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：2009-07-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2020-03-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：2012-01-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2002.01125.x
更新日期：2002-12-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/sj.icb.7100109
更新日期：2007-11-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1987-12-01 00:00:00
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journal_title：Immunology and cell biology
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更新日期：2014-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2000-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 临床试验,杂志文章,多中心研究
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更新日期：2014-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2003.t01-1-01170.x
更新日期：2003-08-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1997-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1997-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：2016-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1990-12-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12031
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