Abstract：
:Mucosal mast cells (MMCs) have an important role in allergic inflammation, and effective antagonists are required for their regulation. To discover a possible mechanism of controlling the activation of MMCs, we investigated the expression and function of syntaxin4, one of the soluble membrane N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, in RBL-2H3 cells, which is a rat mucosal mast cell line. Syntaxin4 silencing was induced by transfection of short interfering RNAs (siRNAs). Syntaxin4 was knocked down in mast cells at both the mRNA and protein levels. The release of granule contents that are involved in inflammation, such as histamine and hexosaminidase, was significantly suppressed by the gene silencing of syntaxin4. Silencing of this gene was also induced in the trachea and bronchi of rats by intratracheal application of the siRNAs using an atelocollagen delivery system. The activation of MMCs, which was monitored by the level of rat mast cell protease-II (RMCPII) in the bronchoalveolar lavage fluid (BALF), was inhibited, and asthmatic airway constriction was prevented by administration of the syntaxin/atelocollagen complex. These results indicate that siRNAs targeting syntaxin4 can stabilize mucosal mast cells and may have beneficial therapeutic effects on the asthmatic response.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Liu S,Nugroho AE,Shudou M,Maeyama Kdoi
10.1038/icb.2011.41subject
Has Abstractpub_date
2012-03-01 00:00:00pages
337-45issue
3eissn
0818-9641issn
1440-1711pii
icb201141journal_volume
90pub_type
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pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：1999-06-01 00:00:00
