Abstract：
:Acute rejection is the major determinant for the long-term survival of donor liver after liver transplantation (LT). The aim of this study was to examine the therapeutic potential of interleukin (IL)-10-FasL-overexpressing immature dendritic cells (imDCs) to induce local immunosuppression in liver grafts. imDCs derived from donors were transduced by lentiviral vectors expressing human IL-10 and/or Fas ligand (FasL) gene(s), and the expression of surface molecules and the ability to induce T-cell proliferation were measured. imDCs were intraperitoneally injected into recipient rats as a model of LT to examine the rejection grade [Banff rejection activity index (RAI)], liver functions [Alanine aminotransferase, Aspartate aminotransferase (AST) and total bilirubin (TBIL)] and post-transplant survival. IL-10 and FasL co-transduction of imDCs induced a greater reduction in CD80, CD86 and major histocompatibility complex class II (MHC II) expression, as well as T-cell proliferation, but increased levels of IL-10 and FasL in culture supernatants compared with mono-transduced or untransduced imDCs (P < 0.05). The infusion of co-transduced imDCs in LT recipients reduced RAI scores, decreased plasma AST and TBIL, and prolonged survival compared with mono-transduced or untransduced imDC-treated liver allografts. These findings demonstrated that the transfusion of IL-10-FasL/imDCs enhanced immune tolerance and prolonged the survival of liver allografts after LT. The immunomodulatory activity of IL-10- and FasL-modified imDCs might be a new therapeutic approach to prevent organ rejection in clinical transplantation.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Chen L,Zhang L,Zhu Z,He W,Gao L,Zhang W,Liu J,Huang Adoi
10.1111/imcb.12252subject
Has Abstractpub_date
2019-09-01 00:00:00pages
714-725issue
8eissn
0818-9641issn
1440-1711journal_volume
97pub_type
杂志文章abstract：:Mammalian barrier surfaces, including the skin, lung and intestine, are constantly exposed to environmental stimuli, including beneficial and pathogenic microbes, dietary substances and non-organic materials. At these anatomical sites it is essential to maintain barrier integrity to promote tissue homeostasis and prev...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
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doi：10.1038/icb.2015.88
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2016.16
更新日期：2016-07-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/imcb.12346
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1996.18
更新日期：1996-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2003-10-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.9
更新日期：1993-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1996.85
更新日期：1996-12-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1990.15
更新日期：1990-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2000.00883.x
更新日期：2000-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2011.114
更新日期：2012-03-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2012.59
更新日期：2013-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.4
更新日期：1997-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2002-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.55
更新日期：1994-10-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1999-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1997-02-01 00:00:00
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更新日期：2015-05-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2007-10-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2005-08-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2000.00921.x
更新日期：2000-08-01 00:00:00
abstract：:Th-cell differentiation is highly influenced by the local cytokine environment. Although cytokines such as IL-12 and IL-4 are known to polarize the Th-cell response towards Th1 or Th2, respectively, it is not known whether these cytokines instruct the developmental fate of uncommitted Th cells or select cells that hav...
journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
abstract：:Two related folate antagonists aminopterin (AMN) and methotrexate (MTX) were used to produce drug-antibody conjugates and their tumouricidal effects compared in vitro and in vivo. Active ester derivatives were produced with the use of N-hydroxysuccinimide (NHS) and covalently coupled to monoclonal antibodies (MoAb) re...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1987.57
更新日期：1987-12-01 00:00:00
