Abstract：
:Peroxidase-conjugated anti-surface immunoglobulin (sIg) was used quantitatively to monitor endocytosis of crosslinked sIg on murine B lymphocytes. The role of biochemical second messengers in the initiation of endocytosis was assessed by employing several inhibitors. A novel peroxidase detection system was used and temperature-dependent decreases in sIg density on immunoperoxidase-labelled murine lymphocytes were monitored. Metabolic inhibitors as well as colchicine and cytochalasin D were utilized to confirm that the internalization of sIg could be blocked by classical inhibitors of the endocytosis process. The role of tyrosine kinase activity was established by the fact that endocytosis was significantly reduced with 100 micrograms/mL genistein. Experiments using EGTA or 1,2-bis(beta-aminophenoxy)ethane-N-N,N'-tetraacetic acid (BAPTA) to chelate Ca2+ indicated that Ca2+ plays little role in endocytosis. Likewise, protein kinase C (PKC) was not found to be involved in endocytosis, as activation of PKC with 50 ng/mL phorbol 12-myristate 13-acetate, or inhibition of the enzyme with 1 nmol/L or 5 nmol/L staurosporin, did not modulate endocytosis. Taken together, results suggested that ligand-induced endocytosis of antigen receptors is mediated primarily through localized membrane events and is not dependent upon the classical B lymphocyte activation signals, such as the biochemical events in the inositol phosphate cascade.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Shuler RL,Owen CSdoi
10.1038/icb.1993.1subject
Has Abstractpub_date
1993-02-01 00:00:00pages
1-11eissn
0818-9641issn
1440-1711journal_volume
71 ( Pt 1)pub_type
杂志文章abstract：:NKT cells are a minor subset of T cells that have important roles in controlling immune responses in disease states including cancer, autoimmunity and pathogenic infections. In contrast to conventional T cells, NKT cells express an invariant TCR and respond to glycolipids presented by CD1d. In this study, we sought to...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01396.x
更新日期：2006-02-01 00:00:00
abstract：:The three modes of antibody production, natural, T independent (TI) and T dependent (TD) are conserved among vertebrate species suggesting an important role for each in protection against pathogens. Here, I use an artificial 'universe' to argue that the three modes of antibody production represent layers that evolved ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.95
更新日期：1997-12-01 00:00:00
abstract：:Many specific antiviral and antitumour immune responses have been attributed to the protective effects of antigen-specific CD8+ cytotoxic T lymphocytes (CTL). Recognition of virus infected or tumour cells by CTL requires presentation of at least one peptide epitope from a virus or tumour-specific antigen by the releva...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2003.01130.x
更新日期：2003-02-01 00:00:00
abstract：:It has been described that polymorphonuclear neutrophils (PMNs) enhance the replication of CC-chemokine receptor 5/macrophage-tropic (R5) HIV in cultures of monocyte-derived macrophages (MDMs). In this study, the inhibitory effect of glycyrrhizin (GL) on R5 HIV replication influenced by PMNs was investigated in MDM cu...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2009.40
更新日期：2009-10-01 00:00:00
abstract：:The multimammate rat Mastomys natalensis, when infected with the filarial parasite Acanthocheilonema viteae, develops amicrofilaraemia. Worm recovery and the duration and intensity of microfilaraemia were analysed and related to the humoral and cellular immune responses of the host by using an antibody-dependent cell-...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1990.3
更新日期：1990-02-01 00:00:00
abstract：:For the past 20 years footrot vaccines have evolved from simple bacterins to highly specific recombinant DNA (rDNA) fimbrial vaccines. The development of these vaccines has left a trail of discoveries, challenges and solutions; these processes continue as we move closer to understanding the requirements of a footrot v...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1993.53
更新日期：1993-10-01 00:00:00
abstract：:Today's generally accepted view of the self-nonself discrimination was voiced by Miller(1) in 2004 in a thought-provoking essay. In spite of its popularity, this position has its limitations, which are analyzed here with a view toward establishing an interactive discussion that hopefully will culminate in agreed upon ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2008.96
更新日期：2009-02-01 00:00:00
abstract：:By hybridization with a human interleukin-6 (IL-6) cDNA fragment a corresponding ruminant (ovine) cDNA was isolated from a lipopolysaccharide (LPS)-stimulated alveolar macrophage library. The nucleotide sequence of the cDNA and the predicted amino acid sequence of the protein showed significant homology to the human a...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.39
更新日期：1993-08-01 00:00:00
abstract：:Mycobacterial disease remains a serious global health problem. Tuberculosis causes more than 2 million deaths a year, and leprosy is still a cause of severe disability in many parts of the world. As a result of the study of individuals with marked susceptibility to usually nonpathogenic mycobacteria, as well as case-c...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/j.1440-1711.2006.01420.x
更新日期：2006-04-01 00:00:00
abstract：:Irradiated malaria sporozoites induce better protection than viable untreated sporozoites. We observed early differences between irradiated and viable untreated sporozoites in priming responses in vivo to a protective CD8 T-cell epitope, pb9, of the circumsporozoite protein of Plasmodium berghei. Sporozoites were proc...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01325.x
更新日期：2005-06-01 00:00:00
abstract：:Early pregnancy factor (EPF), an extracellular chaperonin 10 homologue, has immunosuppressive and growth factor properties. In order to carry out more extensive studies on the in vivo characteristics of EPF, a recombinant form of the molecule has been prepared. Recombinant human EPF (rEPF) was expressed in Escherichia...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2000.00951.x
更新日期：2000-12-01 00:00:00
abstract：:There is little evidence that cutaneous dendritic cells (DC), including epidermal Langerhans cells (LC), can induce immunity to UV radiation (UVR)-induced skin tumours. Here, it is shown that cells within skin can induce protective antitumour immunity against a UVR-induced fibrosarcoma. Transplantation of the skin ove...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2001.00967.x
更新日期：2001-02-01 00:00:00
abstract：:Ikaros, Aiolos and Helios encode zinc finger transcription factors that are important regulators of lymphoid development and differentiation. These proteins are involved in the control of gene expression and when associated with nuclear complexes, participate in nucleosome remodeling. Because differential splicing pro...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2003.01159.x
更新日期：2003-06-01 00:00:00
abstract：:The present study demonstrates that SRBC can be opsonized with untreated human serum such that lysis by active complement components is minimal but sufficient opsonization occurs to permit high rates of complement-mediated phagocytosis. Phagocytosis of SRBC opsonized with 2% whole human serum by human monocyte-derived...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2001.01027.x
更新日期：2001-10-01 00:00:00
abstract：:Three murine monoclonal antibodies (BC1, BC2 and BC3) were developed against human milk fat globule membrane (HMFGM). By immunoperoxidase staining, it was found that the antigenic determinants had a predominant distribution in breast cancer tissue. In addition, the antibodies reacted preferentially with mucin derived ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1989.29
更新日期：1989-06-01 00:00:00
abstract：:Regulatory mechanisms in mucosal secretions and tissues recognize antigens and attenuate pro-inflammatory cytokine responses. Here, we asked whether human beta-defensin 3 (HBD3) serves as an upstream suppressor of cytokine signaling that binds and attenuates pro-inflammatory cytokine responses to recombinant hemagglut...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2008.56
更新日期：2008-11-01 00:00:00
abstract：:The germinal centre reaction (GCR) is a fundamental component of the immune response to T-dependent antigens, during which the immunoglobulin (Ig) genes of B cells experience somatic hypermutation and selection. A maximum-likelihood method on DNA sequence data from 16 individual germinal centres was used to infer that...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.1998.00753.x
更新日期：1998-08-01 00:00:00
abstract：:This study investigated the role of gamma delta T cells in experimental allergic encephalomyelitis (EAE), a chronic inflammatory disease of the central nervous system (CNS) that resembles multiple sclerosis. The strategy was to assess the effect on EAE of TCR peptide immunization directed against V gamma 6 T cells, sh...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.14
更新日期：1997-02-01 00:00:00
abstract：:In their recent publication, Kuczma and colleagues have provided evidence that advances the relationship between naive T cells, T cell anergy and T regulatory cells. Their findings strengthen the understanding of how these T cell subsets function in relation to self and microbiota-derived epitopes to promote and maint...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12424
更新日期：2020-12-04 00:00:00
abstract：:Acute rejection is the major determinant for the long-term survival of donor liver after liver transplantation (LT). The aim of this study was to examine the therapeutic potential of interleukin (IL)-10-FasL-overexpressing immature dendritic cells (imDCs) to induce local immunosuppression in liver grafts. imDCs derive...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12252
更新日期：2019-09-01 00:00:00
abstract：:T lymphocytes are characterized by the use of structurally diverse TCR. The discovery of subsets of canonical T cells that have structurally homogeneous TCR presents an enigma: What antigens do these T cells recognize, and how does their antigen specificity relate to their functions? One subset of canonical T cells is...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/j.0818-9641.2004.01257.x
更新日期：2004-06-01 00:00:00
abstract：:It is known today that the immune system is influenced by various types of psychological and physiological stressors, including physical activity. It is well known that physical activity can influence neuropeptide levels both in the central nervous system as well as in peripheral blood. The reported changes of immune ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/j.1440-1711.2000.t01-10-.x
更新日期：2000-10-01 00:00:00
abstract：:The identification of the genes that make up rearranged immunoglobulin genes is critical to many studies. For example, the enumeration of mutations in immunoglobulin genes is important for the prognosis of chronic lymphocytic leukemia, and this requires the accurate identification of the germline genes from which a pa...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/sj.icb.7100144
更新日期：2008-02-01 00:00:00
abstract：:Urinary tract infections are a major problem in human medicine for which better understanding of native immune defenses may reveal new pathways for therapeutic intervention. Tamm-Horsfall glycoprotein (THP), the most abundant urinary protein, interacts with bacteria including uropathogenic Escherichia coli (UPEC) as w...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2017.63
更新日期：2017-11-01 00:00:00
abstract：:The cellular effects of biodiesel emissions particulate matter (BDEP) and petroleum diesel emissions particulate matter (PDEP) were compared using a human airway cell line, A549. At concentrations of 25 microg/ml, diesel particulate matter induced the formation of multinucleate cells. In cells treated with a mixture o...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/sj.icb.7100109
更新日期：2007-11-01 00:00:00
abstract：:There has been a tremendous explosion in the area of DNA vaccine research over the last 4 years, particularly in relation to antiviral vaccines. This report discusses the development and application of this new technology with regard to parasitic infections. Progress has been made towards the development of a vaccine ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1997.58
更新日期：1997-08-01 00:00:00
abstract：:The evolutionarily conserved catabolic process of autophagy involves the degradation of cytoplasmic components through lysosomal enzymes. Basal levels of autophagy maintain cellular homeostasis and under stress conditions high levels of autophagy are induced. It is often under such stress conditions that high levels o...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2014.85
更新日期：2015-01-01 00:00:00
abstract：:A derivative of ammonia caramel colour (AC) is known to induce a selective lymphopenia in rats. Accordingly, the haematological effects were studied in mice of oral administration in drinking water of 2-acetyl-4(5)-tetrahydroxybutylimidazole (THI), the component of AC responsible for lymphopenia. Initially five groups...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.51
更新日期：1988-10-01 00:00:00
abstract：:γδ T cells producing interleukin-17A (γδT17) are thought to develop spontaneously in the thymus and to be maintained in the periphery. Previous studies suggested a role for T-helper 17 (Th17) cells in the maintenance of γδT17 via the expression of transforming growth factor-β1 (TGFβ1). However, we have previously foun...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2016.94
更新日期：2017-03-01 00:00:00
abstract：:The central pathogenic feature of AIDS is the dramatic loss of CD4+ lymphocytes. Despite more than a decade of intense research, the exact mechanism by which HIV causes this is still not understood. A major model for T cell depletion, proposed originally by Ameison and Capron in a report published in 1991, is that HIV...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1999.00798.x
更新日期：1999-02-01 00:00:00
