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Production of a recombinant form of early pregnancy factor that can prolong allogeneic skin graft survival time in rats.

Abstract：

:Early pregnancy factor (EPF), an extracellular chaperonin 10 homologue, has immunosuppressive and growth factor properties. In order to carry out more extensive studies on the in vivo characteristics of EPF, a recombinant form of the molecule has been prepared. Recombinant human EPF (rEPF) was expressed in Escherichia coli using the plasmid pGEX-2T expression system. Potency of rEPF in vitro in the rosette inhibition test, the bioassay for EPF, was equivalent to that of native EPF (nEPF), purified from human platelets, and synthetic EPF (sEPF). However, the half-life of activity (50% decrease in the log value) in serum, following i.p. injection, was significantly decreased (3.2 h, compared with nEPF 6.2 days, sEPF 5.8 days). This was thought to be due to modification of the N-terminus of the recombinant molecule inhibiting binding to serum carrier proteins. Because EPF can modify Th1 responses, the ability of the recombinant molecule to suppress allogeneic graft rejection was investigated. Following skin grafts from Lewis rats to DA rats and vice versa, rEPF was delivered locally at the graft site and the effect on survival time of the allografts noted. Results demonstrated that rEPF treatment significantly prolonged skin graft survival time by as much as 55% in stringent models of transplantation across major histocompatibility barriers.

journal_name

Immunol Cell Biol

journal_title

Immunology and cell biology

authors

Morton H,McKay DA,Murphy RM,Somodevilla-Torres MJ,Swanson CE,Cassady AI,Summers KM,Cavanagh AC

doi

10.1046/j.1440-1711.2000.00951.x

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

603-7

issue

6

eissn

0818-9641

issn

1440-1711

pii

icb951

journal_volume

78

pub_type

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