Abstract：
:Major histocompatibility complex (MHC) class I heavy chain/beta(2)m heterodimers assemble with antigenic peptides through interactions with peptide-loading complex proteins, including tapasin and ERp57. In human cells, a cysteine residue within tapasin (C95) has been shown to form a covalent bond with ERp57. In this study, we focused on the effect of this tapasin amino-acid residue in mouse cells expressing the MHC class I molecule H2-K(d). We showed that a large disulfide-bonded complex was present in the mouse cells that included ERp57, tapasin, and K(d). Furthermore, in mouse cells, unlike human cells, we found that tapasin mutated at C95 can participate in a non-covalent complex with ERp57. Comparison of our findings to earlier findings with a human molecule (HLA-B(*)4402) also revealed that a tapasin C95 mutation has a stronger effect on the maturation and stability of K(d) than HLA-B(*)4402. Overall, our results characterize the influence of this tapasin cysteine residue on the stable surface expression of a mouse MHC class I molecule and reveal differences in tapasin C95 interactions and effects between mouse and human systems.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Simone LC,Wang X,Tuli A,Solheim JCdoi
10.1038/icb.2009.59subject
Has Abstractpub_date
2010-01-01 00:00:00pages
57-62issue
1eissn
0818-9641issn
1440-1711pii
icb200959journal_volume
88pub_type
杂志文章abstract：:The discovery of functional long noncoding RNAs (lncRNAs) coupled with the ever-increasing accessibility of genomic and transcriptomic technology has led to an explosion of functional and mechanistic investigation and discovery into what was once dismissed as junk DNA. Over the past decade, a significant number of lnc...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2015.2
更新日期：2015-03-01 00:00:00
abstract：:The iscom is a delivery system, designed for both parenteral and mucosal modes of administration, for both antigens and adjuvants, components which are interchangeable. By the parenteral route a prominent systemic Th1 type of response is evoked, but the mucosal immunoglobulin A (IgA) response was insignificant. Intran...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1998.00756.x
更新日期：1998-08-01 00:00:00
abstract：:Human plasmacytoid dendritic cells (pDCs) were considered to be a phenotypically and functionally homogeneous cell population; however, recent analyses indicate potential heterogeneity. This is of major interest, given their importance in the induction of anti-viral responses and their role in creating immunologically...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2015.116
更新日期：2016-05-01 00:00:00
abstract：:The peroxisome proliferator-activated receptors (PPAR) are ligand-activated transcription factors. There are three genes that code for the PPAR isoforms: PPARalpha, PPARbeta and PPARgamma. In the present review, studies characterizing the various PPAR isoforms are discussed. Peroxisome proliferator-activated receptor ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2000.00921.x
更新日期：2000-08-01 00:00:00
abstract：:See also: Outstanding Observation by Wirasinha et al., Short Communication by Barnes & Cyster. ...
journal_title：Immunology and cell biology
pub_type： 评论,杂志文章
doi：10.1111/imcb.12027
更新日期：2018-04-01 00:00:00
abstract：:The role of plasmacytoid dendritic cells (pDC) in anti-HIV immunity is mostly represented by the production of type I IFN in response to HIV infection in vitro and in vivo. This production is decreased in HIV-1 infected patients at the time of primary infection and during chronic disease in association with progressio...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/j.1440-1711.2005.01394.x
更新日期：2005-10-01 00:00:00
abstract：:The surface phenotype of T cells reflects both their relative maturity and their activation state. To determine the pattern of surface markers characteristic of activated T cells, purified mature T cells were stimulated in vitro for periods of 0.5-5 days with Concanavalin-A (Con-A) or phorbol myristic acetate (PMA) an...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.39
更新日期：1988-08-01 00:00:00
abstract：:The gut mucosal system must fulfil conflicting roles in suppressing immune responses against orally fed antigens (tolerance) while still retaining the ability to respond to potential enteric pathogens. It must also, to a large degree, not mount an immune response against commensal enteric bacteria and the administrati...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2000.00883.x
更新日期：2000-02-01 00:00:00
abstract：:The possible role and influence of tumour necrosis factor-alpha (TNF-alpha) in the human mixed lymphocyte culture (MLC) reaction was investigated. Polyclonal rabbit sera to recombinant human-TNF-alpha (rhTNF-alpha) consistently inhibited the MLC, and this could be reversed by simultaneous addition of rhTNF-alpha to th...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1992.15
更新日期：1992-04-01 00:00:00
abstract：:Mast cells (MC) can undergo significant changes in number and phenotype; these alterations result in the differential expression of growth factors and cytokines. Kit ligand (KL; stem cell factor) is produced by mesenchymal cells, and in the liver by biliary epithelial cells. Recent studies suggest that KL, and its rec...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01368.x
更新日期：2005-12-01 00:00:00
abstract：:Influx of Ca2+ via Ca2+ channels is the major step triggering exocytosis of pituitary somatotropes to release growth hormone (GH). Voltage-gated Ca2+ and K+ channels, the primary determinants of the influx of Ca2+, are regulated by GH-releasing hormone (GHRH) through G-protein-coupled intracellular signalling systems....
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2000.00917.x
更新日期：2000-08-01 00:00:00
abstract：:The Australian populations of rainbow trout (Onchorhynchus mykiss) and Atlantic salmon (Salmo salar) were found to have similar immunological responses to local strains of Vibrio anguillarum as those reported for the more genetically diverse populations of these fish and strains of V. anguillarum found in the Northern...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1992.51
更新日期：1992-12-01 00:00:00
abstract：:Infection of mice with the flaviviruses West Nile virus (WNV) and Murray Valley encephalitis (MVE) induces cytolytic T-cell responses which are highly cross-reactive on target cells infected with heterologous flaviviruses. Of C57BL/6 mice infected with low doses (10(2)-10(6) PFU) of either virus, 30-40% develop enceph...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.0818-9641.2004.01227.x
更新日期：2004-04-01 00:00:00
abstract：:The utility of T-cell receptor (TCR) transgenic mice in medical research has been considerable, with applications ranging from basic biology all the way to translational and clinical investigations. Crossing of TCR transgenic mice with either recombination-activating gene (RAG)-1 or RAG-2 knockouts is frequently used ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/imcb.12033
更新日期：2018-07-01 00:00:00
abstract：:γδ T cells producing interleukin-17A (γδT17) are thought to develop spontaneously in the thymus and to be maintained in the periphery. Previous studies suggested a role for T-helper 17 (Th17) cells in the maintenance of γδT17 via the expression of transforming growth factor-β1 (TGFβ1). However, we have previously foun...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2016.94
更新日期：2017-03-01 00:00:00
abstract：:The ability of a tunicate immunomodulatory protein, tunIL1, to interact with mammalian cells was investigated. TunIL1 is known to regulate inflammatory defence reactions in tunicates and to stimulate the proliferation of mammalian thymocytes. In the current study, tunIL1 was shown to enchance L929 fibroblast prolifera...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1996.3
更新日期：1996-02-01 00:00:00
abstract：:Regulating the strength and class of an immune response requires lymphocytes to act as complex signal integrating "machines", taking information from multiple sources while making decisions that affect the final outcome. Describing and understanding the decision-making behaviour of lymphocytes within the context of th...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/sj.icb.7100061
更新日期：2007-06-01 00:00:00
abstract：:NK cells from three donors with a NK (CD3- CD56+ CD16+) lymphocytosis of unknown aetiology showed differential expression of CD45R0, an isoform of CD45 not expressed by NK cells from normal donors unless stimulated to proliferate in vitro. For donor FC, 60% of NK cells expressed CD45R0 over a 16 month period during wh...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.74
更新日期：1994-12-01 00:00:00
abstract：:As previously reported by others for immunoglobulin (Ig)G, we observed that IgA can induce interleukin (IL)-10 expression in human monocytes. In this study, we explored the molecular mechanisms of IL-10 induction by IgA in monocytes and monocyte-derived dendritic cells (MD-DCs). Monomeric IgA induced IL-10 production ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2009.120
更新日期：2010-05-01 00:00:00
abstract：:Dendritic cells (DCs) are professional antigen-presenting cells that consist of functionally and phenotypically heterogeneous populations. Monocyte-derived DCs (moDCs) are a DC subset that have been attracting increasing interest owing to their potent influence on adaptive immune function and their rapid accumulation ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2016.104
更新日期：2017-03-01 00:00:00
abstract：:Urinary tract infections are a major problem in human medicine for which better understanding of native immune defenses may reveal new pathways for therapeutic intervention. Tamm-Horsfall glycoprotein (THP), the most abundant urinary protein, interacts with bacteria including uropathogenic Escherichia coli (UPEC) as w...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2017.63
更新日期：2017-11-01 00:00:00
abstract：:Deoxyspergualin (DOSP) is a new immunosuppressive agent which probably inhibits various functions of monocytes, B cells and T cells. We examined the effects of deoxyspergualin on cellular cytotoxicity, including cytotoxic T lymphocyte (CTL) mediated killer, natural killer (NK) cell and lymphokine activated killer (LAK...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1991.26
更新日期：1991-06-01 00:00:00
abstract：:Two related folate antagonists aminopterin (AMN) and methotrexate (MTX) were used to produce drug-antibody conjugates and their tumouricidal effects compared in vitro and in vivo. Active ester derivatives were produced with the use of N-hydroxysuccinimide (NHS) and covalently coupled to monoclonal antibodies (MoAb) re...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1987.57
更新日期：1987-12-01 00:00:00
abstract：:Inhaled allergens are known for their immediate and ongoing effects in the respiratory tract (RT). In this report, we track inhaled antigen in normal mice for 7 days and find that while it is cleared from the airways, inhaled antigen persists in peripheral lung tissue and the draining lymph nodes (DLNs). The persisten...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2010.18
更新日期：2010-08-01 00:00:00
abstract：:Recombinant DNA techniques were used to clone, construct and express the bifunctional molecule FV/IL-2. The FV/IL-2 is a single-chain 37 kDa fusion protein expressed in Escherichia coli under control of the strong T7 bacteriophage promoter in the expression vector pT7-7-FV-IL-2. The fused gene fragment FV-IL-2 contain...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1994.42
更新日期：1994-08-01 00:00:00
abstract：:The V region antigenic determinants (idiotopes (Ids)) of antibodies (Abs) have been suggested to be involved in regulating the immune system. Certain diseases such as diabetes mellitus have recently been associated with a disequilibrium between Id(+) and anti-Id Abs. However, it is unknown how Abs carrying complementa...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2009.118
更新日期：2010-07-01 00:00:00
abstract：:By hybridization with a human interleukin-6 (IL-6) cDNA fragment a corresponding ruminant (ovine) cDNA was isolated from a lipopolysaccharide (LPS)-stimulated alveolar macrophage library. The nucleotide sequence of the cDNA and the predicted amino acid sequence of the protein showed significant homology to the human a...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.39
更新日期：1993-08-01 00:00:00
abstract：:Retinoic acid (RA) in the steady state enhances induction of Foxp3(+) regulatory T (Treg) cells and inhibits differentiation of Th1 and Th17 cells, thereby maintaining tolerance, but can in inflammatory conditions promote effector Th1 and Th17 cells that mediate inflammation. IL-17-producing γδ T cells have recently b...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2016.39
更新日期：2016-09-01 00:00:00
abstract：:Alzheimer's disease (AD) is characterized by amyloid beta (Aβ) accumulation, tau pathology and neuroinflammation. Recently, there has been considerable interest in the role of neuroinflammation in directly contributing to the progression of AD. Studies in mice and humans have identified a role for microglial cells, th...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1111/imcb.12301
更新日期：2020-01-01 00:00:00
abstract：:The mouse peritoneal cavity contains a unique population of B cells (Bla) with a high IgM/low IgD ratio, CD5+ (Ly1), MAC-1 + phenotype. These cells arise early in ontogeny, utilize a limited repertoire of immunoglobulin V genes, produce polyreactive IgM antibodies and have been implicated as the source of many auto-re...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1995.66
更新日期：1995-10-01 00:00:00
