Abstract:
:The possible role and influence of tumour necrosis factor-alpha (TNF-alpha) in the human mixed lymphocyte culture (MLC) reaction was investigated. Polyclonal rabbit sera to recombinant human-TNF-alpha (rhTNF-alpha) consistently inhibited the MLC, and this could be reversed by simultaneous addition of rhTNF-alpha to the culture medium. Under optimal cell culture conditions, the exogenous addition of 1000 U/mL of rh TNF-alpha had a variable but generally small enhancing effect on the MLC reaction. Additional studies have clearly highlighted the importance of MLC assay conditions in influencing the effect of exogenous rhTNF-alpha. Enhancement of the MLC reaction was only consistently observed under suboptimal cell culture conditions. While the present study clearly supports an important role for TNF in controlling the proliferative response in the human MLC reaction it also highlights the influence of in vitro tissue culture conditions in determining the effect of exogenous TNF-alpha in this assay.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Matthews SJ,Sullivan JSdoi
10.1038/icb.1992.15subject
Has Abstractpub_date
1992-04-01 00:00:00pages
107-10eissn
0818-9641issn
1440-1711journal_volume
70 ( Pt 2)pub_type
杂志文章abstract::zeta (CD3-zeta) and gamma (Fc epsilon RI gamma) chains associate with CD16, the low affinity receptor for IgG (Fc gamma RIII) on human NK cells and are essential for the cell surface expression of CD16 and for CD16-mediated effector functions. This study has investigated whether, on NK cells, molecules other than CD16...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1994.13
更新日期:1994-02-01 00:00:00
abstract::The pathogenesis of autoimmune disease remains an enigma. Here, the condition is analysed from an evolutionary standpoint, and the thesis developed that viruses, in particular retroviruses, are important to our evolution, and that their inappropriate re-expression by repetitive (? ischaemic) cell damage in individuals...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1997.78
更新日期:1997-10-01 00:00:00
abstract::Natural killer (NK) cells are potent immune effector cells that can respond to infection and cancer, as well as allowing maternal adaptation to pregnancy. In response to malignant transformation or pathogenic invasion, NK cells can secrete cytokine and may be directly cytolytic, as well as exerting effects indirectly ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2010.78
更新日期:2011-02-01 00:00:00
abstract::Natural killer (NK) cells are part of the first line defense against tumors, parasites and virus-infected cells. Therefore, factors that control NK-cell numbers and their function are important. CD27 is constitutively expressed on NK cells and its expression correlates with sequential phases in NK-cell development, di...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2010.171
更新日期:2011-10-01 00:00:00
abstract::The recognition of T cell receptors (TCR) by purified major histocompatibility complex (MHC) class II-peptide complexes in the absence of costimulatory signals leads to the induction of T cell non-responsiveness or anergy. In a recent study using human T cell clones, it was observed that prolonged incubation of restin...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1996.13
更新日期:1996-02-01 00:00:00
abstract::Genetic variants of interleukin 2 (IL-2) and its receptor are associated with murine and human susceptibility to Type 1 diabetes, yet the role of IL-2 in controlling pancreatic islet-reactive T cells is unknown. Here, we develop a model where IL-2 deficiency precipitates a breakdown of self-tolerance and progression t...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100049
更新日期:2007-06-01 00:00:00
abstract::Respiratory syncytial virus causes lower respiratory tract infections in infancy and old age, affecting also immunocompromised patients. The viral fusion protein is an important vaccine candidate eliciting antibody and cell-mediated immune responses. CD8(+) cytotoxic T lymphocytes (CTLs) are known to have a role in bo...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2012.43
更新日期:2012-11-01 00:00:00
abstract::Interferon regulatory factor (IRF) family members impart cell-type specificity to toll-like receptor (TLR) signalling, and we recently identified a role for IRF6 in TLR2 signalling in epithelial cells. TLR3 has a well-characterized role in wound healing in the skin, and here, we examined TLR3-dependent IRF6 functions ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2015.77
更新日期:2015-10-01 00:00:00
abstract::Productive immune responses to T-cell-dependent antigens ultimately generate two long-lived compartments: memory B cells and bone marrow-resident plasma cells, which both arise from within germinal centres. The ability of a B-cell clone to populate these effector compartments requires its descendents to outcompete tho...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/sj.icb.7100148
更新日期:2008-02-01 00:00:00
abstract::Gαq, the α-subunit of Gq protein, is ubiquitously expressed in mammalian cells. It initially attracted attention for its physiological significance in cardiovascular system. In recent years, studies have also indicated the important roles of Gαq in regulating immunity, supplying us a new insight into the mechanism of ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2015.13
更新日期:2015-08-01 00:00:00
abstract::Rats primed with bovine myelin (BM) in complete Freunds adjuvant, develop acute experimental autoimmune encephalomyelitis (EAE). We have previously described that intraperitoneal administration prior to the active induction of the disease of a bovine synaptosomal fraction (BSF) and BM were effective ways of suppressin...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2004.01200.x
更新日期:2004-02-01 00:00:00
abstract::Peripheral tolerance mechanisms limit autoimmunity by constitutively eliminating self-reactive CD8+ T cells from the periphery in a process called deletion. Previous work has demonstrated that this deletion process is mediated by BIM-dependent apoptotic death due to transcriptional induction of the Bim gene. Currently...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.53
更新日期:2016-10-01 00:00:00
abstract::Adult Nematospiroides dubius excretory-secretory (ES) products were collected from worms cultured in vitro, radiolabelled and separated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). The components were characterized and assessed for molecular weight (MW) after autoradiography and gel staining, for a...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1989.16
更新日期:1989-04-01 00:00:00
abstract::There is a need for non-living adjuvant vectors that will allow a full range of local and systemic immune responses to orally administered purified antigens. Here we describe our experience with lipophilic immune-stimulating complexes (ISCOMs) containing the saponin adjuvant Quil A. When given orally, ISCOMs containin...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.1998.00749.x
更新日期:1998-06-01 00:00:00
abstract::During a chronic infection such as tuberculosis, the pool of tissue dendritic cells (DC) must be renewed by recruitment of both circulating DC progenitors and monocytes (Mo). However, the microenvironment of the inflammatory site affects Mo differentiation. As DC are critical for initiating a Mycobacterium tuberculosi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2010.22
更新日期:2010-10-01 00:00:00
abstract::Adoptive transfer of tumor-specific cytotoxic T cells is a promising advance in cancer therapy. Similarly, checkpoint inhibition has shown striking clinical results in some patients. Here we combine adoptive cell transfer with ablation of the checkpoint protein Src homology 2-domain-containing phosphatase 1 (SHP-1, Pt...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.45
更新日期:2016-09-01 00:00:00
abstract::A general criterion is formulated to decide if the recycling of B cells exists in germinal centre (GC) reactions. The criterion is independent of the selection and affinity maturation process and based solely on total centroblast population arguments. An experimental test is proposed to verify whether the criterion is...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2002.01052.x
更新日期:2002-02-01 00:00:00
abstract::CD4(+) T cells are activated upon recognition of peptide antigen in the context of MHC class II molecules, expressed by specialized APC. In this study, we show that CD4(+) T cells cross-compete for antigenic complexes on the surface of APC, inhibiting activation of other potentially reactive T cells of the same and di...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.0818-9641.2004.01233.x
更新日期:2004-04-01 00:00:00
abstract::One of the major limitations for cancer immunotherapy is related to the frequent existence of an intra-tumoral immunosuppressive environment, to which STAT3 (Signal transducer and activator of transcription-3) activation in tumor and dendritic cells (DCs) are believed to contribute. In this study, we tested the hypoth...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2008.27
更新日期:2008-08-01 00:00:00
abstract::Mycobacterial disease remains a serious global health problem. Tuberculosis causes more than 2 million deaths a year, and leprosy is still a cause of severe disability in many parts of the world. As a result of the study of individuals with marked susceptibility to usually nonpathogenic mycobacteria, as well as case-c...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1111/j.1440-1711.2006.01420.x
更新日期:2006-04-01 00:00:00
abstract::CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leucocytes, and which plays a crucial role in the function of these cells. On T cells the extracellular domain of CD45 is expressed in several different isoforms, and the particular isoform(s) expressed depends ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1997.68
更新日期:1997-10-01 00:00:00
abstract::The mechanisms involved in the inhibition of growth of a human B lymphoma cell line, B104, by anti-MHC class II antibodies (Ab) were compared with those in anti-IgM Ab-induced B104 growth inhibition. Two anti-MHC class II Ab, L227 and 2.06, inhibited the growth of B104 cells, although 2.06, but not L227, needed to be ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1994.31
更新日期:1994-06-01 00:00:00
abstract::A long-term culture (LTC) system has been established that supports the continuous production of dendritic cells (DC) from haemopoietic cells present in the culture. The production of cells depends on the presence of an intact stromal cell layer containing a mixture of fibroblasts and endothelial cells. Cells are shed...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.1999.00851.x
更新日期:1999-10-01 00:00:00
abstract::Previous data from this laboratory have shown that fluorescein-specific cytotoxic T cells derived from naive mice are capable of distinguishing between different isomeric forms of the fluorescein hapten, as well as between the iodoacetamido-, isothiocyanate- and dichlorotriazinyl amino-fluorescein derivatives. In this...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1995.24
更新日期:1995-04-01 00:00:00
abstract::Viral infections can disturb the functions of adipose tissues and thus result in metabolic diseases. Polyinosinic-polycytidylic acid (poly(I:C)), a synthetic analog of viral double-stranded RNA, induces innate antiviral responses by mimicking viral infection through the activation of pattern recognition receptors (PRR...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.57
更新日期:2016-10-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most prevalent cancers worldwide with rising mortality rates predicted in the coming decades. In light of this, there is a continued need for improvement in our understanding of CRC biology and the development of novel treatment options. Tumor-derived extracellular vesicles (tEVs)...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1111/imcb.12038
更新日期:2018-03-25 00:00:00
abstract::Dendritic cell (DC)-based immunotherapy has recently demonstrated a great potential for clinical applications; however, additional progress in the methods of tumor-specific antigen delivery to DCs is necessary for the further development of anti-tumor vaccines. To this end, a capsid-optimized adeno-associated virus se...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2013.74
更新日期:2014-02-01 00:00:00
abstract::Using 'knockin' mice to track and ablate dendritic cells (DCs) expressing notably the langerin (Cd207) gene, it has been possible to identify five DC subsets within the skin and to assess whether functional specialization exists among them. The present review summarizes recent information concerning the phenotype and ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2010.34
更新日期:2010-05-01 00:00:00
abstract::The HECT-type E3 ligase Itch is increasingly being shown to have a vital role in immune regulation. Itch deficiency leads to deleterious inflammatory disorders both in mice and humans. By adding ubiquitin to the key signaling intermediates, Itch functions as a critical regulator of lymphocyte-cell activation, differen...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2014.118
更新日期:2015-05-01 00:00:00
abstract::Selection of conventional CD4(+) or CD8(+) T cells is usually driven by the interaction of double-positive CD4(+)CD8(+) thymocytes with epithelial cells. Here, we demonstrate preferential selection of CD8(+) thymocytes from in vitro differentiation of CD4(+)CD8(+) double-positive thymocytes exhibiting the characterist...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2013.94
更新日期:2014-04-01 00:00:00