Abstract:
:A long-term culture (LTC) system has been established that supports the continuous production of dendritic cells (DC) from haemopoietic cells present in the culture. The production of cells depends on the presence of an intact stromal cell layer containing a mixture of fibroblasts and endothelial cells. Cells are shed from foci of dividing cells in contact with the stromal cell matrix. They resemble DC in terms of morphology and cell surface marker expression. The LTC can be derived from different lymphoid tissues, but most success has been achieved with murine spleen. Different LTC vary in capacity to produce immunostimulatory DC. Some LTC produce DC that are very effective APC and can stimulate both mixed lymphocyte and antigen-specific T cell responses. The DC produced in others are weak APC. Different LTC appear to produce DC reflecting different stages of maturation or development, reflected by different phenotypic and functional characteristics. The production of cells within LTC occurs independently of added cytokines and is dependent on maintenance of the stromal cell layer and the presence of a subset of smaller progenitor cells. Long-term cultures remain a valuable source of cells for study of DC development and function.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
O'Neill HC,Ni K,Wilson Hdoi
10.1046/j.1440-1711.1999.00851.xsubject
Has Abstractpub_date
1999-10-01 00:00:00pages
434-41issue
5eissn
0818-9641issn
1440-1711pii
icb851journal_volume
77pub_type
杂志文章,评审abstract::Genetic variants of interleukin 2 (IL-2) and its receptor are associated with murine and human susceptibility to Type 1 diabetes, yet the role of IL-2 in controlling pancreatic islet-reactive T cells is unknown. Here, we develop a model where IL-2 deficiency precipitates a breakdown of self-tolerance and progression t...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100049
更新日期:2007-06-01 00:00:00
abstract::The multimammate rat Mastomys natalensis, when infected with the filarial parasite Acanthocheilonema viteae, develops amicrofilaraemia. Worm recovery and the duration and intensity of microfilaraemia were analysed and related to the humoral and cellular immune responses of the host by using an antibody-dependent cell-...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1990.3
更新日期:1990-02-01 00:00:00
abstract::Somatostatin inhibits in vitro lymphocyte proliferative responses from a variety of species including human, mouse and rat. The immunoinhibitory effects of somatostatin are thought to involve binding to specific cell surface somatostatin receptors on immunocompetent cells. This report describes an in vivo immunoinhibi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.13
更新日期:1993-04-01 00:00:00
abstract::Plasma cells play a pivotal role in the immune system and are responsible for the synthesis and release of immunoglobulins. Numerous in vitro culture experiments on the yolk sac demonstrated the generation of mature cells of the myeloid and lymphoid lineages under appropriate culture conditions. However, there are no ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2003.t01-1-01178.x
更新日期:2003-10-01 00:00:00
abstract::Mucosal mast cells (MMCs) have an important role in allergic inflammation, and effective antagonists are required for their regulation. To discover a possible mechanism of controlling the activation of MMCs, we investigated the expression and function of syntaxin4, one of the soluble membrane N-ethylmaleimide-sensitiv...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2011.41
更新日期:2012-03-01 00:00:00
abstract::FOXP3+ regulatory T (Treg) cells are indispensable for immune homeostasis, but their study in humans is complicated by heterogeneity within Treg, the difficulty in purifying Tregs using surface marker expression (e.g. CD25) and the transient expression of FOXP3 by activated effector cells. Here, we report that express...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2015.90
更新日期:2016-03-01 00:00:00
abstract::Tumour cell heterogeneity is probably a principal cause of treatment failure and represents a formidable barrier for effective antibody-targeted chemotherapy. Idarubicin (Ida), a more potent and less cardiotoxic analogue of daunomycin, has been demonstrated to specifically target and eradicate homogeneous, cloned, mur...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.19
更新日期:1993-06-01 00:00:00
abstract::Arginase-1 (Arg-1)-expressing M2-like macrophages are associated with Th2-skewed immune responses, allergic airway pathology, ectopic B16 melanoma cancer growth in murine models, and can be induced by Oncostatin M (OSM) transient overexpression in vivo. Here, we compare OSM to the gp130-cytokine IL-6 in mediating macr...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.1035
更新日期:2018-03-01 00:00:00
abstract::CD8(+) T cells kill pancreatic β-cells in a cell-cell contact-dependent mechanism in the non-obese diabetic mouse. CD4(+) T lymphocytes are also able to kill pancreatic β-cells, but they do not directly contact β-cells and may use another cell type as the actual cytotoxic cell. Natural killer (NK) cells could have thi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2011.16
更新日期:2012-02-01 00:00:00
abstract::Multiple levels of regulation of collagenase (matrix metalloproteinase 1; MMP-1), have been demonstrated in a clonal rat epithelial cell line (A5P/B10). Secreted enzyme could not be demonstrated in culture medium from A5P/B10 cells but, using antibodies specific for collagenase, the enzyme was detected within the cyto...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.4
更新日期:1993-02-01 00:00:00
abstract::During an allergic inflammatory response in the airway, if a failure of the epithelial cell barrier occurs before the systemic immune response is triggered by allergens, more allergens can invade. Using a rat model of asthma, we previously found that mucosal mast cells, which localise to the epithelial layer of the ai...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2010.90
更新日期:2011-02-01 00:00:00
abstract::The broadest definition of dendritic cells (DCs) is white blood cells that can take up antigen, process it and then present antigen-derived peptides to activate cognate naive T cells. Although this definition is by no means perfect, it is nevertheless now textbook. The source of frustration more recently has focused o...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2008.28
更新日期:2008-07-01 00:00:00
abstract::Infection of mice with the flaviviruses West Nile virus (WNV) and Murray Valley encephalitis (MVE) induces cytolytic T-cell responses which are highly cross-reactive on target cells infected with heterologous flaviviruses. Of C57BL/6 mice infected with low doses (10(2)-10(6) PFU) of either virus, 30-40% develop enceph...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.0818-9641.2004.01227.x
更新日期:2004-04-01 00:00:00
abstract::Natural suppressor (NS) cell line (Clone 59) was established from the bone marrow of adult C3H/Hej mice in the presence of WEHI-3 conditioned media. Clone 59 cells suppressed the generation of cytotoxic T lymphocytes from normal mouse spleen. This suppression was seen at a responder-to-suppressor cell ratio of 1000:1 ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1995.51
更新日期:1995-08-01 00:00:00
abstract::Latex beads, 250 and 610 nm in diameter, and parapox virus isolated from ecthyma in sheep, were injected into intestinal loops containing either jejunal or ileal Peyer's patches (PP) of 3-4 week old calves. Uptake of latex and parapox virus was restricted to the ileal PP, 30-60 min after injection. The latex beads see...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1988.35
更新日期:1988-08-01 00:00:00
abstract::Intravenous (i.v.) immunization of mice with recombinant vaccinia viruses stimulated the highest antibody and cytotoxic T lymphocyte (CTL) responses when i.v., intraperitoneal (i.p.), intranasal (i.n.), footpad (f.p.) and tail scarification (t.s.) routes were compared. Intraperitoneal immunization of mice resulted in ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1989.48
更新日期:1989-10-01 00:00:00
abstract::Dendritic cell (DC)-based immunotherapy has recently demonstrated a great potential for clinical applications; however, additional progress in the methods of tumor-specific antigen delivery to DCs is necessary for the further development of anti-tumor vaccines. To this end, a capsid-optimized adeno-associated virus se...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2013.74
更新日期:2014-02-01 00:00:00
abstract::Human regulatory T cells (Tregs) expressing the transcription factor FoxP3 play indispensable roles for the maintenance of immunological self-tolerance and immune homeostasis. In this review, we discuss immunological characteristics of human FoxP3(+) Tregs and to what extent they are similar to or different from the m...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2010.137
更新日期:2011-03-01 00:00:00
abstract::Regulating the strength and class of an immune response requires lymphocytes to act as complex signal integrating "machines", taking information from multiple sources while making decisions that affect the final outcome. Describing and understanding the decision-making behaviour of lymphocytes within the context of th...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100061
更新日期:2007-06-01 00:00:00
abstract::Fundamentally new approaches are required for the development of vaccines to pre-empt and protect against emerging and pandemic influenzas. Current strategies involve post-emergent homotypic vaccines that are modelled upon select circulating 'seasonal' influenzas, but cannot induce cross-strain protection against newl...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2011.38
更新日期:2012-02-01 00:00:00
abstract::It has been described that polymorphonuclear neutrophils (PMNs) enhance the replication of CC-chemokine receptor 5/macrophage-tropic (R5) HIV in cultures of monocyte-derived macrophages (MDMs). In this study, the inhibitory effect of glycyrrhizin (GL) on R5 HIV replication influenced by PMNs was investigated in MDM cu...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2009.40
更新日期:2009-10-01 00:00:00
abstract::A general criterion is formulated to decide if the recycling of B cells exists in germinal centre (GC) reactions. The criterion is independent of the selection and affinity maturation process and based solely on total centroblast population arguments. An experimental test is proposed to verify whether the criterion is...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2002.01052.x
更新日期:2002-02-01 00:00:00
abstract::Immunotherapy of tumours using T cells expanded in vitro has met with mixed clinical success suggesting that a greater understanding of tumour/T-cell interaction is required. We used a HPV16E7 oncoprotein-based mouse tumour model to study this further. In this study, we demonstrate that a HPV16E7 tumour passes through...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.0818-9641.2004.01273.x
更新日期:2004-10-01 00:00:00
abstract::Th-cell differentiation is highly influenced by the local cytokine environment. Although cytokines such as IL-12 and IL-4 are known to polarize the Th-cell response towards Th1 or Th2, respectively, it is not known whether these cytokines instruct the developmental fate of uncommitted Th cells or select cells that hav...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2006.01425.x
更新日期:2006-04-01 00:00:00
abstract::The central pathogenic feature of AIDS is the dramatic loss of CD4+ lymphocytes. Despite more than a decade of intense research, the exact mechanism by which HIV causes this is still not understood. A major model for T cell depletion, proposed originally by Ameison and Capron in a report published in 1991, is that HIV...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.1999.00798.x
更新日期:1999-02-01 00:00:00
abstract::Endothelial cell (EC)-neutrophil (PMN) interactions are crucial in the resolution of bacterial infections. Prokaryotic RNA (pRNA) has been reported as a pathogen-associated molecular pattern that is released from bacteria upon death and is able to activate PMN. In this work, we studied the effects of pRNA on EC and in...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12282
更新日期:2019-10-01 00:00:00
abstract::Previous reports have documented that mice bearing plasmacytomas (PC) are suppressed in their ability to mount a primary antibody response, whereas cellular immunity appears to be normal. Studies presented here provide evidence that T cell responsiveness is also depressed in BALB/c mice bearing the Lieberman plasmacyt...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1991.3
更新日期:1991-02-01 00:00:00
abstract::Our earlier studies have demonstrated that gonadotropin-releasing hormone (GnRH) agonists suppress immune system function in female mice. No systematic studies regarding the effect of gender on immune system function following GnRH agonist treatment, however, have been reported. This study, therefore, investigated seq...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1996.18
更新日期:1996-04-01 00:00:00
abstract::Technetium-99 m (99mTc)-labelled conjugates of an anti-fibrin monoclonal antibody, DD-3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD-3B6/22 binds to a D-dimer epitope present on cross-linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a 99mTc-labelled...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1992.22
更新日期:1992-06-01 00:00:00
abstract::There is increasing evidence that a small percentage of individuals exposed to the hepatitis C virus have the capacity to generate a strong cellular immune response against the virus and avoid persistent infection, and perhaps do so repeatedly after re-exposure. This article reviews the evidence that the responses ide...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1111/j.1440-1711.2006.01427.x
更新日期:2006-06-01 00:00:00