当前位置： SCI文献检索 > IMMUNOLOGY AND CELL BIOLOGY期刊下所有文献 > TLR3 drives IRF6-dependent IL-23p19 expression and p19/EBI3 heterodimer formation in keratinocytes.

TLR3 drives IRF6-dependent IL-23p19 expression and p19/EBI3 heterodimer formation in keratinocytes.

Abstract：

:Interferon regulatory factor (IRF) family members impart cell-type specificity to toll-like receptor (TLR) signalling, and we recently identified a role for IRF6 in TLR2 signalling in epithelial cells. TLR3 has a well-characterized role in wound healing in the skin, and here, we examined TLR3-dependent IRF6 functions in human keratinocytes. Primary keratinocytes responded robustly to the TLR3 agonist poly(IC) with upregulation of mRNAs for interferon-β (IFN-β), the interleukin-12 (IL-12) family member IL-23p19 and the chemokines IL-8 and chemokine (C-C motif) ligand 5 (CCL5). Silencing of IRF6 expression enhanced poly(IC)-inducible IFN-β mRNA levels and inhibited poly(IC)-inducible IL-23p19 mRNA expression in primary keratinocytes. Consistent with these data, co-transfection of IRF6 increased poly(IC)-inducible IL-23p19 promoter activity, but inhibited poly(IC)-inducible IFN-β promoter activity in reporter assays. Surprisingly, poly(IC) did not regulate IL-12p40 expression in keratinocytes, suggesting that TLR3-inducible IL-23p19 may have an IL-23-independent function in these cells. The only other IL-12 family member that was strongly poly(IC) inducible was EBI3, which has not been shown to heterodimerize with IL-23p19. Both co-immunoprecipitation and proximity ligation assays revealed that IL-23p19 and EBI3 interact in cells. Co-expression of IL-23p19 and EBI3, as compared with IL-23p19 alone, resulted in increased levels of secreted IL-23p19, implying a functional role for this heterodimer. In summary, we report that IRF6 regulates a subset of TLR3 responses in human keratinocytes, including the production of a novel IL-12 family heterodimer (p19/EBI3). We propose that the TLR3-IRF6-p19/EBI3 axis may regulate keratinocyte and/or immune cell functions in the context of cell damage and wound healing in the skin.

journal_name

Immunol Cell Biol

journal_title

Immunology and cell biology

authors

Ramnath D,Tunny K,Hohenhaus DM,Pitts CM,Bergot AS,Hogarth PM,Hamilton JA,Kapetanovic R,Sturm RA,Scholz GM,Sweet MJ

doi

10.1038/icb.2015.77

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

771-9

issue

9

eissn

0818-9641

issn

1440-1711

pii

icb201577

journal_volume

93

pub_type

杂志文章

相关文献

IMMUNOLOGY AND CELL BIOLOGY文献大全
  • Deoxyspergualin inhibits cytotoxic T lymphocytes but not NK or LAK cells.

    abstract：:Deoxyspergualin (DOSP) is a new immunosuppressive agent which probably inhibits various functions of monocytes, B cells and T cells. We examined the effects of deoxyspergualin on cellular cytotoxicity, including cytotoxic T lymphocyte (CTL) mediated killer, natural killer (NK) cell and lymphokine activated killer (LAK...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1991.26

    authors： Kerr PG,Atkins RC

    更新日期：1991-06-01 00:00:00

  • How adaptive antibodies facilitate the evolution of natural antibodies.

    abstract：:It is shown how Ig specificities, randomly generated in conventional B cells, come to be expressed in the genetically-determinate B1 population. Thus the adaptive antibody population facilitates the evolution of the natural antibody repertoire, in accordance with the Baldwin effect in the evolution of instinct. The ev...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/icb.1996.50

    authors： Anderson RW

    更新日期：1996-06-01 00:00:00

  • Diverse intracellular signalling systems used by growth hormone-releasing hormone in regulating voltage-gated Ca2+ or K channels in pituitary somatotropes.

    abstract：:Influx of Ca2+ via Ca2+ channels is the major step triggering exocytosis of pituitary somatotropes to release growth hormone (GH). Voltage-gated Ca2+ and K+ channels, the primary determinants of the influx of Ca2+, are regulated by GH-releasing hormone (GHRH) through G-protein-coupled intracellular signalling systems....

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1046/j.1440-1711.2000.00917.x

    authors： Chen C,Xu R,Clarke IJ,Ruan M,Loneragan K,Roh SG

    更新日期：2000-08-01 00:00:00

  • Use of CD107-based cell sorting ex vivo to enrich subdominant CD8+ T cells in culture.

    abstract：:CD8+ T lymphocytes are key effectors in the control of viral diseases and some tumours. In general, the majority of CD8+ T cells recognize a few immunodominant epitopes, but in some circumstances, subdominant specificities may be more relevant as targets for vaccines or immunotherapy. Epstein-Barr virus (EBV)-associat...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/sj.icb.7100083

    authors： Moor RJ,Morrison LE,Moss DJ,Tscharke DC

    更新日期：2007-10-01 00:00:00

  • A CD2 high-expressing stress-resistant human plasmacytoid dendritic-cell subset.

    abstract：:Human plasmacytoid dendritic cells (pDCs) were considered to be a phenotypically and functionally homogeneous cell population; however, recent analyses indicate potential heterogeneity. This is of major interest, given their importance in the induction of anti-viral responses and their role in creating immunologically...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.2015.116

    authors： Bryant C,Fromm PD,Kupresanin F,Clark G,Lee K,Clarke C,Silveira PA,Suen H,Brown R,Newman E,Cunningham I,Ho PJ,Gibson J,Bradstock K,Joshua D,Hart DNj

    更新日期：2016-05-01 00:00:00

  • Tumour-specific CTL response requiring interactions of four different cell types and recognition of MHC class I and class II restricted tumour antigens.

    abstract：:This study demonstrates that a syngeneic specific cytotoxic T lymphocyte (CTL) response to a class I major histocompatibility complex (MHC) positive tumour requires dual processing and recognition of tumour antigens. One type of antigen is processed and expressed in association with class I MHC at the surface of intac...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1993.36

    authors： Schirrmacher V,Schild HJ,Gückel B,von Hoegen P

    更新日期：1993-08-01 00:00:00

  • Differential expression of CD45R0 on natural killer (NK) cells in patients with an NK lymphocytosis.

    abstract：:NK cells from three donors with a NK (CD3- CD56+ CD16+) lymphocytosis of unknown aetiology showed differential expression of CD45R0, an isoform of CD45 not expressed by NK cells from normal donors unless stimulated to proliferate in vitro. For donor FC, 60% of NK cells expressed CD45R0 over a 16 month period during wh...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1994.74

    authors： Warren HS,Kinnear BF,Skipsey LJ,Pembrey RG

    更新日期：1994-12-01 00:00:00

  • Functional aspects of iscoms.

    abstract：:The iscom is a delivery system, designed for both parenteral and mucosal modes of administration, for both antigens and adjuvants, components which are interchangeable. By the parenteral route a prominent systemic Th1 type of response is evoked, but the mucosal immunoglobulin A (IgA) response was insignificant. Intran...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1440-1711.1998.00756.x

    authors： Morein F,Bengtsson KL

    更新日期：1998-08-01 00:00:00

  • DNA vaccines for parasitic infections.

    abstract：:There has been a tremendous explosion in the area of DNA vaccine research over the last 4 years, particularly in relation to antiviral vaccines. This report discusses the development and application of this new technology with regard to parasitic infections. Progress has been made towards the development of a vaccine ...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/icb.1997.58

    authors： Kalinna BH

    更新日期：1997-08-01 00:00:00

  • Effect of a tapasin mutant on the assembly of the mouse MHC class I molecule H2-K(d).

    abstract：:Major histocompatibility complex (MHC) class I heavy chain/beta(2)m heterodimers assemble with antigenic peptides through interactions with peptide-loading complex proteins, including tapasin and ERp57. In human cells, a cysteine residue within tapasin (C95) has been shown to form a covalent bond with ERp57. In this s...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.2009.59

    authors： Simone LC,Wang X,Tuli A,Solheim JC

    更新日期：2010-01-01 00:00:00

  • Relevance of necroptosis in cancer.

    abstract：:Resistance to caspase-dependent apoptosis is often responsible for treatment failures in cancer. Finding novel therapeutic strategies that can activate alternative cell death programs appears to be appealing. Necroptosis is a form of programmed necrosis that occurs under caspase-deficient conditions. This alternative ...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/icb.2016.120

    authors： Lalaoui N,Brumatti G

    更新日期：2017-02-01 00:00:00

  • Role for plasmacytoid dendritic cells in anti-HIV innate immunity.

    abstract：:The role of plasmacytoid dendritic cells (pDC) in anti-HIV immunity is mostly represented by the production of type I IFN in response to HIV infection in vitro and in vivo. This production is decreased in HIV-1 infected patients at the time of primary infection and during chronic disease in association with progressio...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1440-1711.2005.01394.x

    authors： Müller-Trutwin M,Hosmalin A

    更新日期：2005-10-01 00:00:00

  • Antigen fusion with C3d3 augments or inhibits humoral immunity to AAV genetic vaccines in a transgene-dependent manner.

    abstract：:Genetic fusion of tandem repeats of the complement molecule C3d has been shown to considerably enhance immune responses to genetic vaccines. We have investigated the applicability of this approach to augment humoral immune responses toward vaccines delivered by recombinant adeno-associated virus (AAV) vectors. C3d(3)-...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.2009.92

    authors： Logan GJ,Wang L,Zheng M,Coppel RL,Alexander IE

    更新日期：2010-02-01 00:00:00

  • Heterogeneity, functional specialization and differentiation of monocyte-derived dendritic cells.

    abstract：:Dendritic cells (DCs) are professional antigen-presenting cells that consist of functionally and phenotypically heterogeneous populations. Monocyte-derived DCs (moDCs) are a DC subset that have been attracting increasing interest owing to their potent influence on adaptive immune function and their rapid accumulation ...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1038/icb.2016.104

    authors： Chow KV,Sutherland RM,Zhan Y,Lew AM

    更新日期：2017-03-01 00:00:00

  • Mechanisms involved in the inhibition of growth of a human B lymphoma cell line, B104, by anti-MHC class II antibodies.

    abstract：:The mechanisms involved in the inhibition of growth of a human B lymphoma cell line, B104, by anti-MHC class II antibodies (Ab) were compared with those in anti-IgM Ab-induced B104 growth inhibition. Two anti-MHC class II Ab, L227 and 2.06, inhibited the growth of B104 cells, although 2.06, but not L227, needed to be ...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1994.31

    authors： Higaki Y,Hata D,Kanazashi S,Horiguchi Y,Yamaoka K,Ohshima Y,Kim KM,Heike T,Mayumi M

    更新日期：1994-06-01 00:00:00

  • The good, the (not so) bad and the ugly of immune homeostasis in melanoma.

    abstract：:Within the immune system multiple mechanisms balance the need for efficient pathogen recognition and destruction with the prevention of tissue damage by excessive, inappropriate or even self-targeting (auto)immune reactions. This immune homeostasis is a tightly regulated system which fails during tumor development, of...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章,评审

    doi：10.1111/imcb.12001

    authors： da Gama Duarte J,Woods K,Andrews MC,Behren A

    更新日期：2018-05-01 00:00:00

  • Shining LIGHT on the metabolic role of the cytokine TNFSF14 and the implications on hepatic IL-6 production.

    abstract：:The cytokine Tumor Necrosis Factor Superfamily member 14, TNFSF14 (or LIGHT), is a controversial player in numerous diseases. We investigated the role of endogenously expressed TNFSF14 in diet-induced obesity in vivo. Firstly, we studied the effects of Tnfsf14 ablation on the development of obesity, glucose intoleranc...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1111/imcb.1002

    authors： Saunders BM,Rudnicka C,Filipovska A,Davies S,Ward N,Hricova J,Schlaich MP,Matthews VB

    更新日期：2018-01-01 00:00:00

  • Clearance of Brugia pahangi microfilariae in immunized mice.

    abstract：:Active Brugia pahangi microfilariae (Mf) were injected into naive male BALB/c mice intraperitoneally. Microfilaraemia was studied for 28 days; and Mf circulated in blood in optimum numbers from 3 to 14 days. Anti-Mf response was assessed by the rate of disappearance of Mf from blood as well as their absence from visce...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1988.43

    authors： Rajasekariah GR,Puri PM,Chandrashekar R,Subrahmanyam D

    更新日期：1988-10-01 00:00:00

  • Effects of IL-10- and FasL-overexpressing dendritic cells on liver transplantation tolerance in a heterotopic liver transplantation rat model.

    abstract：:Acute rejection is the major determinant for the long-term survival of donor liver after liver transplantation (LT). The aim of this study was to examine the therapeutic potential of interleukin (IL)-10-FasL-overexpressing immature dendritic cells (imDCs) to induce local immunosuppression in liver grafts. imDCs derive...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1111/imcb.12252

    authors： Chen L,Zhang L,Zhu Z,He W,Gao L,Zhang W,Liu J,Huang A

    更新日期：2019-09-01 00:00:00

  • Regulation of human Th9 differentiation by type I interferons and IL-21.

    abstract：:Interleukin (IL)-9-producing CD4(+) T cells are a novel subset of T helper (Th) cells that develops independently of the Th1, Th2, Th17 and regulatory T-cell lineages. Similar to the murine model, transforming growth factor (TGF)-beta and IL-4 directed human naive CD4(+) T cells to produce IL-9. Whereas IL-4 suppresse...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.2010.53

    authors： Wong MT,Ye JJ,Alonso MN,Landrigan A,Cheung RK,Engleman E,Utz PJ

    更新日期：2010-08-01 00:00:00

  • Lymphocyte activation as measured by interleukin-2 receptor expression to gluten fraction 111 in coeliac disease.

    abstract：:Lymphocyte activation was examined by interleukin-2 (IL-2) receptor expression on peripheral blood mononuclear cells from coeliac and control subjects. Purified T cells were incubated with gluten fraction 111 (a known toxic peptide for coeliac subjects), soyabean hydrolysate (an unrelated hydrolysed food antigen), and...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1990.22

    authors： Penttila IA,Gibson CE,Forrest BD,Cummins AG,LaBrooy JT

    更新日期：1990-06-01 00:00:00

  • In vivo inhibition of the rat primary antibody response to antigenic stimulation by somatostatin.

    abstract：:Somatostatin inhibits in vitro lymphocyte proliferative responses from a variety of species including human, mouse and rat. The immunoinhibitory effects of somatostatin are thought to involve binding to specific cell surface somatostatin receptors on immunocompetent cells. This report describes an in vivo immunoinhibi...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1993.13

    authors： Eglezos A,Andrews PV,Helme RD

    更新日期：1993-04-01 00:00:00

  • Heterogeneity in heat shock protein genes in Leishmania isolates.

    abstract：:Leishmaniasis, a group of visceral and cutaneous diseases, is caused by parasites belonging to one genus comprising approximately 13 different species. Many methods including serological, biochemical and molecular biological techniques have been used by various workers to characterize these different species and isola...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1046/j.1440-1711.1998.00731.x

    authors： Arora SK,Kapoor GS,Sehgal S

    更新日期：1998-04-01 00:00:00

  • An antigen valence theory to explain the evolution and organization of the humoral immune response.

    abstract：:The three modes of antibody production, natural, T independent (TI) and T dependent (TD) are conserved among vertebrate species suggesting an important role for each in protection against pathogens. Here, I use an artificial 'universe' to argue that the three modes of antibody production represent layers that evolved ...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1997.95

    authors： Hodgkin PD

    更新日期：1997-12-01 00:00:00

  • Microglial cells from psychologically stressed mice as an accelerator of cerebral cryptococcosis.

    abstract：:Severe stress decreases the resistance of hosts exposed to microbial infections. As compared with two groups of control mice (normal mice, food-and-water-deprived mice [FWD mice]), restraint-stressed mice (RST mice) were shown to be greatly susceptible to intracerebral growth of Cryptococcus neoformans. The susceptibi...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1111/j.1440-1711.2006.01466.x

    authors： Shimoda M,Jones VC,Kobayashi M,Suzuki F

    更新日期：2006-12-01 00:00:00

  • Molecular associations involving CD16, CD45 and zeta and gamma chains on human natural killer cells.

    abstract：:zeta (CD3-zeta) and gamma (Fc epsilon RI gamma) chains associate with CD16, the low affinity receptor for IgG (Fc gamma RIII) on human NK cells and are essential for the cell surface expression of CD16 and for CD16-mediated effector functions. This study has investigated whether, on NK cells, molecules other than CD16...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1994.13

    authors： Altin JG,Pagler EB,Kinnear BF,Warren HS

    更新日期：1994-02-01 00:00:00

  • Tamm-Horsfall glycoprotein engages human Siglec-9 to modulate neutrophil activation in the urinary tract.

    abstract：:Urinary tract infections are a major problem in human medicine for which better understanding of native immune defenses may reveal new pathways for therapeutic intervention. Tamm-Horsfall glycoprotein (THP), the most abundant urinary protein, interacts with bacteria including uropathogenic Escherichia coli (UPEC) as w...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.2017.63

    authors： Patras KA,Coady A,Olson J,Ali SR,RamachandraRao SP,Kumar S,Varki A,Nizet V

    更新日期：2017-11-01 00:00:00

  • Multiple levels of post-transcriptional regulation of collagenase (matrix metalloproteinase 1) in an epithelial cell line.

    abstract：:Multiple levels of regulation of collagenase (matrix metalloproteinase 1; MMP-1), have been demonstrated in a clonal rat epithelial cell line (A5P/B10). Secreted enzyme could not be demonstrated in culture medium from A5P/B10 cells but, using antibodies specific for collagenase, the enzyme was detected within the cyto...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1993.4

    authors： Whitelock JM,Paine ML,Gibbins JR,Kefford RF,O'Grady RL

    更新日期：1993-02-01 00:00:00

  • In silico simulations suggest that Th-cell development is regulated by both selective and instructive mechanisms.

    abstract：:Th-cell differentiation is highly influenced by the local cytokine environment. Although cytokines such as IL-12 and IL-4 are known to polarize the Th-cell response towards Th1 or Th2, respectively, it is not known whether these cytokines instruct the developmental fate of uncommitted Th cells or select cells that hav...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1111/j.1440-1711.2006.01425.x

    authors： Jansson A,Fagerlind M,Karlsson D,Nilsson P,Cooley M

    更新日期：2006-04-01 00:00:00

  • Production of a polyspecific human monoclonal antibody reacting with an epidermal antigen.

    abstract：:A clone of cells secreting an antibody to an epidermal antigen was generated from a patient with a blistering skin lesion. Although produced by fusion of human lymphocytes to a HAT-sensitive myeloma, this clone of cells did not have characteristics of a hybridoma. A true hybridoma was produced by fusion of this clone ...

    journal_title：Immunology and cell biology

    pub_type： 杂志文章

    doi：10.1038/icb.1988.30

    authors： Hohmann AW,Coleman M,Comacchio RM,Skinner JM,Bradley J

    更新日期：1988-01-01 00:00:00