Abstract：
:Lipid rafts are small laterally mobile microdomains that are highly enriched in lymphocyte signaling molecules. GM1 gangliosides are a common lipid raft component and have been shown to be important in many T-cell functions. The aggregation of specific GM1 lipid rafts can control many T-cell activation events, including their novel association with T-cell integrins. We found that clustering GM1 lipid rafts can regulate beta1 integrin function. This was apparent through increased resistance to shear flow-dependent detachment of T cells adherent to the alpha4beta1 and alpha5beta1 integrin ligand fibronectin (FN). Adhesion strengthening as a result of clustering GM1 enriched lipid rafts correlated with increased cellular rigidity and morphology through the localization of cortical F-actin, the resistance to shear-induced cell stretching, and an increase in the surface area and symmetry of the contact area between the cell surface and adhesive substrate. Furthermore, clustering GM1 lipid rafts could initiate integrin 'inside-out' signaling mechanisms. This was seen through increased integrin-cytoskeleton associations and enhanced soluble binding of FN and VCAM-1, suggesting the induction of high-affinity integrin conformations. The activation of these adhesion-strengthening characteristics appears to be specific for the aggregation of GM1 lipid rafts as the aggregation of the heterogeneous raft-associated molecule CD59 failed to activate these functions. These findings indicate a novel mechanism to signal to beta1 integrins and to activate adhesion-strengthening processes.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Mitchell JS,Brown WS,Woodside DG,Vanderslice P,McIntyre BWdoi
10.1038/icb.2008.103subject
Has Abstractpub_date
2009-05-01 00:00:00pages
324-36issue
4eissn
0818-9641issn
1440-1711pii
icb2008103journal_volume
87pub_type
杂志文章abstract：:After positive selection, the newly differentiated single-positive (SP) thymocytes undergo negative selection to eliminate autoreactive T cells, functional maturation to acquire immunocompetence and egress capability. To investigate whether the intracellular reduction/oxidation (redox) balance has an important role on...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2013.57
更新日期：2013-11-01 00:00:00
abstract：:Human cytomegalovirus (HCMV), the β-herpesvirus prototype, has evolved a wide spectrum of mechanisms to counteract host immunity. Among them, HCMV uses cellular captured genes encoding molecules capable of interfering with the original host function or of fulfilling new immunomodulatory tasks. Here, we report on UL7, ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2011.55
更新日期：2011-10-01 00:00:00
abstract：:It is shown how Ig specificities, randomly generated in conventional B cells, come to be expressed in the genetically-determinate B1 population. Thus the adaptive antibody population facilitates the evolution of the natural antibody repertoire, in accordance with the Baldwin effect in the evolution of instinct. The ev...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1996.50
更新日期：1996-06-01 00:00:00
abstract：:Tumour cell heterogeneity is probably a principal cause of treatment failure and represents a formidable barrier for effective antibody-targeted chemotherapy. Idarubicin (Ida), a more potent and less cardiotoxic analogue of daunomycin, has been demonstrated to specifically target and eradicate homogeneous, cloned, mur...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1993.19
更新日期：1993-06-01 00:00:00
abstract：:Non-toxic agents that target intracellular signalling pathways in apoptosis may have potential therapeutic use in many diseases. One such agent is the transition metal Zn, a dietary cytoprotectant and anti-oxidant, which stimulates cell proliferation and suppresses apoptosis. Zn is maintained in discrete subcellular p...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1999.00825.x
更新日期：1999-06-01 00:00:00
abstract：:Human plasmacytoid dendritic cells (pDCs) were considered to be a phenotypically and functionally homogeneous cell population; however, recent analyses indicate potential heterogeneity. This is of major interest, given their importance in the induction of anti-viral responses and their role in creating immunologically...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2015.116
更新日期：2016-05-01 00:00:00
abstract：:Natural suppressor (NS) cell line (Clone 59) was established from the bone marrow of adult C3H/Hej mice in the presence of WEHI-3 conditioned media. Clone 59 cells suppressed the generation of cytotoxic T lymphocytes from normal mouse spleen. This suppression was seen at a responder-to-suppressor cell ratio of 1000:1 ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1995.51
更新日期：1995-08-01 00:00:00
abstract：:A general criterion is formulated to decide if the recycling of B cells exists in germinal centre (GC) reactions. The criterion is independent of the selection and affinity maturation process and based solely on total centroblast population arguments. An experimental test is proposed to verify whether the criterion is...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2002.01052.x
更新日期：2002-02-01 00:00:00
abstract：:Active Brugia pahangi microfilariae (Mf) were injected into naive male BALB/c mice intraperitoneally. Microfilaraemia was studied for 28 days; and Mf circulated in blood in optimum numbers from 3 to 14 days. Anti-Mf response was assessed by the rate of disappearance of Mf from blood as well as their absence from visce...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.43
更新日期：1988-10-01 00:00:00
abstract：:This article attempts to assemble theoretical-teleological argument to explore possible answers to the question of why class I MHC molecules bind smaller peptides than class II MHC molecules and the associated question of why the size of peptides binding to class I molecules is approaching the limit of the self-non-se...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1995.15
更新日期：1995-02-01 00:00:00
abstract：:The NK gene complex (NKC) on mouse chromosome 6 encodes receptors that are expressed on NK cells, such as Ly49H, and is involved in regulating NK cell control of virus infections, such as murine cytomegalovirus (MCMV). In the present study, we investigated the level of allelic heterogeneity in NKC loci in populations ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2005.01311.x
更新日期：2005-04-01 00:00:00
abstract：:Antigen presentation by enterocytes isolated from the rat small bowel was studied by using T cell proliferation, and immunoregulatory function in an antigen-driven culture system, as indicator systems. Lymph node T cells obtained from rats immunized with ovalbumin (OA) failed to divide when cultured for 4 days in the ...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1990.52
更新日期：1990-12-01 00:00:00
abstract：:Rats primed with bovine myelin (BM) in complete Freunds adjuvant, develop acute experimental autoimmune encephalomyelitis (EAE). We have previously described that intraperitoneal administration prior to the active induction of the disease of a bovine synaptosomal fraction (BSF) and BM were effective ways of suppressin...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2004.01200.x
更新日期：2004-02-01 00:00:00
abstract：:Cloned Th1 cells, but not Th2 cells, specific for malaria antigen, produce nitric oxide (NO) when activated with specific antigen or Con A. Furthermore, NO inhibits proliferation of, and production of IL-2 and IFN-gamma by, Th1 but not Th2 cells. Here, it is demonstrated that the inhibition of Th1 cell proliferation b...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.23
更新日期：1997-04-01 00:00:00
abstract：:Cells with a low density of parasite-specific antigens on their surface are postulated to be susceptible to a cell-mediated attack but not to effector mechanisms normally activated following the binding of specific antibody to the infected cell. It is further postulated that such infected cells normally induce a cell-...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1992.44
更新日期：1992-10-01 00:00:00
abstract：:This paper reports the production and characterization of three monoclonal antibodies (mAb) recognizing ovine vascular cell adhesion molecule-1 (VCAM-1). The mAb were raised against sheep umbilical vein endothelial cells (ShUVEC) and flow cytometric analysis demonstrated that one mAb, QE4G9, was cross-reactive with hu...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.85
更新日期：1997-12-01 00:00:00
abstract：:MicroRNAs (miRNAs) are abundant small molecules of ≈ 22 nucleotides that reprogram gene expression by targeting mRNA degradation and translation disruption. An emerging concept implicates miRNA coupling with transcription factors in myeloid-based development of dendritic cells, monocytes and granulocytes, as well as f...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2011.74
更新日期：2012-07-01 00:00:00
abstract：:The discovery of functional long noncoding RNAs (lncRNAs) coupled with the ever-increasing accessibility of genomic and transcriptomic technology has led to an explosion of functional and mechanistic investigation and discovery into what was once dismissed as junk DNA. Over the past decade, a significant number of lnc...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.2015.2
更新日期：2015-03-01 00:00:00
abstract：:Th-cell differentiation is highly influenced by the local cytokine environment. Although cytokines such as IL-12 and IL-4 are known to polarize the Th-cell response towards Th1 or Th2, respectively, it is not known whether these cytokines instruct the developmental fate of uncommitted Th cells or select cells that hav...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2006.01425.x
更新日期：2006-04-01 00:00:00
abstract：:Natural killer (NK) cells are part of the first line defense against tumors, parasites and virus-infected cells. Therefore, factors that control NK-cell numbers and their function are important. CD27 is constitutively expressed on NK cells and its expression correlates with sequential phases in NK-cell development, di...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2010.171
更新日期：2011-10-01 00:00:00
abstract：:The 120-kDa protein product of the c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic protein that is especially abundant in the thymus, indicating an important role for Cbl in thymic signalling. c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain, a C3HC4 RING finger motif, multiple...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.1998.00768.x
更新日期：1998-10-01 00:00:00
abstract：:Genetic fusion of tandem repeats of the complement molecule C3d has been shown to considerably enhance immune responses to genetic vaccines. We have investigated the applicability of this approach to augment humoral immune responses toward vaccines delivered by recombinant adeno-associated virus (AAV) vectors. C3d(3)-...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2009.92
更新日期：2010-02-01 00:00:00
abstract：:Follicular dendritic cells (FDC) play a crucial role in the regulation of humoral immunity. They are believed to be responsible for long-term persistence of antibody, due to their role in antibody response induction and their ability to retain antigen in immunogenic form for long periods. In this article, a regulatory...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2017.55
更新日期：2017-10-01 00:00:00
abstract：:During a chronic infection such as tuberculosis, the pool of tissue dendritic cells (DC) must be renewed by recruitment of both circulating DC progenitors and monocytes (Mo). However, the microenvironment of the inflammatory site affects Mo differentiation. As DC are critical for initiating a Mycobacterium tuberculosi...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2010.22
更新日期：2010-10-01 00:00:00
abstract：:The surface phenotype of T cells reflects both their relative maturity and their activation state. To determine the pattern of surface markers characteristic of activated T cells, purified mature T cells were stimulated in vitro for periods of 0.5-5 days with Concanavalin-A (Con-A) or phorbol myristic acetate (PMA) an...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1988.39
更新日期：1988-08-01 00:00:00
abstract：:The peroxisome proliferator-activated receptors (PPAR) are ligand-activated transcription factors. There are three genes that code for the PPAR isoforms: PPARalpha, PPARbeta and PPARgamma. In the present review, studies characterizing the various PPAR isoforms are discussed. Peroxisome proliferator-activated receptor ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1046/j.1440-1711.2000.00921.x
更新日期：2000-08-01 00:00:00
abstract：:The gut mucosal system must fulfil conflicting roles in suppressing immune responses against orally fed antigens (tolerance) while still retaining the ability to respond to potential enteric pathogens. It must also, to a large degree, not mount an immune response against commensal enteric bacteria and the administrati...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2000.00883.x
更新日期：2000-02-01 00:00:00
abstract：:Heterogeneity of T cells is a hallmark of a successful adaptive immune response, harnessing the vast diversity of antigen-specific T cells into a coordinated evolution of effector and memory outcomes. The T cell receptor (TCR) repertoire is highly diverse to account for the highly heterogeneous antigenic world. During...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.2016.16
更新日期：2016-07-01 00:00:00
abstract：:Herpesviruses are an ancient, ubiquitous family of DNA viruses, most of which share a lifestyle of latently or persistently infecting a young host, and spreading to infect a new host a generation later. Most herpesviruses interfere with antigen presentation via the MHC class I-restricted pathway of antigen presentatio...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/icb.1996.85
更新日期：1996-12-01 00:00:00
abstract：:Previous data from this laboratory have shown that fluorescein-specific cytotoxic T cells derived from naive mice are capable of distinguishing between different isomeric forms of the fluorescein hapten, as well as between the iodoacetamido-, isothiocyanate- and dichlorotriazinyl amino-fluorescein derivatives. In this...
journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1995.24
更新日期：1995-04-01 00:00:00
