Abstract:
:Human cytomegalovirus (HCMV), the β-herpesvirus prototype, has evolved a wide spectrum of mechanisms to counteract host immunity. Among them, HCMV uses cellular captured genes encoding molecules capable of interfering with the original host function or of fulfilling new immunomodulatory tasks. Here, we report on UL7, a novel HCMV heavily glycosylated transmembrane protein, containing an Ig-like domain that exhibits remarkable amino acid similarity to CD229, a cell-surface molecule of the signalling lymphocyte-activation molecule (SLAM) family involved in leukocyte activation. The UL7 Ig-like domain, which is well-preserved in all HCMV strains, structurally resembles the SLAM-family N-terminal Ig-variable domain responsible for the homophilic and heterophilic interactions that trigger signalling. UL7 is transcribed with early-late kinetics during the lytic infectious cycle. Using a mAb generated against the viral protein, we show that it is constitutively shed, through its mucine-like stalk, from the cell-surface. Production of soluble UL7 is enhanced by PMA and reduced by a broad-spectrum metalloproteinase inhibitor. Although UL7 does not hold the ability to interact with CD229 or other SLAM-family members, it shares with them the capacity to mediate adhesion to leukocytes, specifically to monocyte-derived DCs. Furthermore, we demonstrate that UL7 expression attenuates the production of proinflammatory cytokines TNF, IL-8 and IL-6 in DCs and myeloid cell lines. Thus, the ability of UL7 to interfere with cellular proinflammatory responses may contribute to viral persistence. These results enhance our understanding of those HCMV-encoded molecules involved in sustaining the balance between HCMV and the host immune system.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Engel P,Pérez-Carmona N,Albà MM,Robertson K,Ghazal P,Angulo Adoi
10.1038/icb.2011.55subject
Has Abstractpub_date
2011-10-01 00:00:00pages
753-66issue
7eissn
0818-9641issn
1440-1711pii
icb201155journal_volume
89pub_type
杂志文章abstract::Alzheimer's disease (AD) is characterized by amyloid beta (Aβ) accumulation, tau pathology and neuroinflammation. Recently, there has been considerable interest in the role of neuroinflammation in directly contributing to the progression of AD. Studies in mice and humans have identified a role for microglial cells, th...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1111/imcb.12301
更新日期:2020-01-01 00:00:00
abstract::Viral infections can disturb the functions of adipose tissues and thus result in metabolic diseases. Polyinosinic-polycytidylic acid (poly(I:C)), a synthetic analog of viral double-stranded RNA, induces innate antiviral responses by mimicking viral infection through the activation of pattern recognition receptors (PRR...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2016.57
更新日期:2016-10-01 00:00:00
abstract::As host immunological defenses are impaired during HIV infection, it is difficult to elicit good responses when attempting to develop therapeutic vaccines against HIV. To try to solve this situation, adjuvants, particularly cytokines, are currently under evaluation. Owing to the fact that adenosine deaminase (ADA) is ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2009.53
更新日期:2009-11-01 00:00:00
abstract::This paper reports the production and characterization of three monoclonal antibodies (mAb) recognizing ovine vascular cell adhesion molecule-1 (VCAM-1). The mAb were raised against sheep umbilical vein endothelial cells (ShUVEC) and flow cytometric analysis demonstrated that one mAb, QE4G9, was cross-reactive with hu...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1997.85
更新日期:1997-12-01 00:00:00
abstract::Mycobacterium tuberculosis uses numerous mechanisms to avoid elimination by the infected host. In this study, we investigated the possibility whether, similar to other pathogens, M. tuberculosis exploits natural CD4+ CD25+ T-regulatory cells (Treg) to suppress the effector function of responding host lymphocytes, thus...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2006.01460.x
更新日期:2006-10-01 00:00:00
abstract::The molecular interactions between the T-cell receptor (TCR) and peptide-MHC (pMHC) have been elucidated in recent years. Nevertheless, the fact that binding of only slightly different ligands by a TCR, or ligation of the same pMHC at different developmental stages of the T cell, can have opposing consequences, contin...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.2001.00994.x
更新日期:2001-04-01 00:00:00
abstract::Technetium-99 m (99mTc)-labelled conjugates of an anti-fibrin monoclonal antibody, DD-3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD-3B6/22 binds to a D-dimer epitope present on cross-linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a 99mTc-labelled...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1992.22
更新日期:1992-06-01 00:00:00
abstract::The effects of endotoxin treatment on host metabolism in tumour-bearing rats were investigated. Metabolism in control rats (non-tumour-bearing) was slightly altered by endotoxin treatment, whereas in tumour-bearing rats a number of biochemical parameters that were initially perturbed by the presence of the tumour had ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1992.1
更新日期:1992-02-01 00:00:00
abstract::Epstein-Barr virus achieves its ubiquitous and uniform epidemiological distribution by a dual strategy of latency to guarantee lifelong persistence and intermittent replication to guarantee transmission. These two functions appear to dictate residence in different cell types: latency in B lymphocytes and replication i...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.1995.79
更新日期:1995-12-01 00:00:00
abstract::Irradiated malaria sporozoites induce better protection than viable untreated sporozoites. We observed early differences between irradiated and viable untreated sporozoites in priming responses in vivo to a protective CD8 T-cell epitope, pb9, of the circumsporozoite protein of Plasmodium berghei. Sporozoites were proc...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/j.1440-1711.2005.01325.x
更新日期:2005-06-01 00:00:00
abstract::Dendritic cells (DCs) orchestrate the innate and adaptive immune systems to induce tolerance and immunity. DC plasticity and subsets are prominent determinants in the regulation of immune responses. Our recent studies suggest that humoral and cellular immunity is regulated by different myeloid DC subsets with distinct...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2010.28
更新日期:2010-05-01 00:00:00
abstract::The 120-kDa protein product of the c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic protein that is especially abundant in the thymus, indicating an important role for Cbl in thymic signalling. c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain, a C3HC4 RING finger motif, multiple...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.1998.00768.x
更新日期:1998-10-01 00:00:00
abstract::The antitumour efficacy of Ida-anti-Ly-2.1 immunoconjugate in combination with murine TNF-alpha (mTNF-alpha) has been evaluated in a disseminated murine thymic lymphoma model E3. This tumour model shows characteristics similar to lymphoma in humans with widely disseminated tumour in major organs. In vitro both idarubi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1997.39
更新日期:1997-06-01 00:00:00
abstract::In their recent publication, Kuczma and colleagues have provided evidence that advances the relationship between naive T cells, T cell anergy and T regulatory cells. Their findings strengthen the understanding of how these T cell subsets function in relation to self and microbiota-derived epitopes to promote and maint...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.12424
更新日期:2020-12-04 00:00:00
abstract::The cellular effects of biodiesel emissions particulate matter (BDEP) and petroleum diesel emissions particulate matter (PDEP) were compared using a human airway cell line, A549. At concentrations of 25 microg/ml, diesel particulate matter induced the formation of multinucleate cells. In cells treated with a mixture o...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100109
更新日期:2007-11-01 00:00:00
abstract::Arginase-1 (Arg-1)-expressing M2-like macrophages are associated with Th2-skewed immune responses, allergic airway pathology, ectopic B16 melanoma cancer growth in murine models, and can be induced by Oncostatin M (OSM) transient overexpression in vivo. Here, we compare OSM to the gp130-cytokine IL-6 in mediating macr...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1111/imcb.1035
更新日期:2018-03-01 00:00:00
abstract::After positive selection, the newly differentiated single-positive (SP) thymocytes undergo negative selection to eliminate autoreactive T cells, functional maturation to acquire immunocompetence and egress capability. To investigate whether the intracellular reduction/oxidation (redox) balance has an important role on...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2013.57
更新日期:2013-11-01 00:00:00
abstract::Listeria monocytogenes is the causative agent of infections like sepsis and meningitis, especially in immunocompromised hosts. Human macrophages are able to phagocytose and digest L. monocytogenes but IL-4 prevents human macrophages from killing the bacteria, the mechanisms of which are unknown. In the present study, ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1711.2003.01196.x
更新日期:2003-12-01 00:00:00
abstract::The ability of a tunicate immunomodulatory protein, tunIL1, to interact with mammalian cells was investigated. TunIL1 is known to regulate inflammatory defence reactions in tunicates and to stimulate the proliferation of mammalian thymocytes. In the current study, tunIL1 was shown to enchance L929 fibroblast prolifera...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1996.3
更新日期:1996-02-01 00:00:00
abstract::The V region antigenic determinants (idiotopes (Ids)) of antibodies (Abs) have been suggested to be involved in regulating the immune system. Certain diseases such as diabetes mellitus have recently been associated with a disequilibrium between Id(+) and anti-Id Abs. However, it is unknown how Abs carrying complementa...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2009.118
更新日期:2010-07-01 00:00:00
abstract::The three modes of antibody production, natural, T independent (TI) and T dependent (TD) are conserved among vertebrate species suggesting an important role for each in protection against pathogens. Here, I use an artificial 'universe' to argue that the three modes of antibody production represent layers that evolved ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1997.95
更新日期:1997-12-01 00:00:00
abstract::zeta (CD3-zeta) and gamma (Fc epsilon RI gamma) chains associate with CD16, the low affinity receptor for IgG (Fc gamma RIII) on human NK cells and are essential for the cell surface expression of CD16 and for CD16-mediated effector functions. This study has investigated whether, on NK cells, molecules other than CD16...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1994.13
更新日期:1994-02-01 00:00:00
abstract::Using 'knockin' mice to track and ablate dendritic cells (DCs) expressing notably the langerin (Cd207) gene, it has been possible to identify five DC subsets within the skin and to assess whether functional specialization exists among them. The present review summarizes recent information concerning the phenotype and ...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2010.34
更新日期:2010-05-01 00:00:00
abstract::A murine monoclonal antibody has been used to characterise a 45,000 Da antigen that is associated with the surface membrane of merozoites of the human malaria parasite Plasmodium falciparum. The antigen is a glycoprotein and incorporates myristic acid. ...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1987.48
更新日期:1987-10-01 00:00:00
abstract::Granzyme B (GrB) plays a well-established intracellular role in cytotoxic lymphocyte (CL)-mediated killing of abnormal cells; however, emerging evidence suggests that it participates in extracellular matrix remodeling and target cell destruction through anoikis. As these processes require the release of GrB from the C...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.2008.98
更新日期:2009-03-01 00:00:00
abstract::Activins and inhibins are members of the transforming growth factor-β superfamily that have been considered crucial regulators of cell processes, such as differentiation, proliferation and apoptosis, in different cell types. Initial studies about the function of activin A in the immune system focused on the regulation...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2011.32
更新日期:2012-02-01 00:00:00
abstract::Influx of Ca2+ via Ca2+ channels is the major step triggering exocytosis of pituitary somatotropes to release growth hormone (GH). Voltage-gated Ca2+ and K+ channels, the primary determinants of the influx of Ca2+, are regulated by GH-releasing hormone (GHRH) through G-protein-coupled intracellular signalling systems....
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1046/j.1440-1711.2000.00917.x
更新日期:2000-08-01 00:00:00
abstract::Somatostatin inhibits in vitro lymphocyte proliferative responses from a variety of species including human, mouse and rat. The immunoinhibitory effects of somatostatin are thought to involve binding to specific cell surface somatostatin receptors on immunocompetent cells. This report describes an in vivo immunoinhibi...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/icb.1993.13
更新日期:1993-04-01 00:00:00
abstract::Mammalian barrier surfaces, including the skin, lung and intestine, are constantly exposed to environmental stimuli, including beneficial and pathogenic microbes, dietary substances and non-organic materials. At these anatomical sites it is essential to maintain barrier integrity to promote tissue homeostasis and prev...
journal_title:Immunology and cell biology
pub_type: 杂志文章,评审
doi:10.1038/icb.2013.2
更新日期:2013-03-01 00:00:00
abstract::Regulating the strength and class of an immune response requires lymphocytes to act as complex signal integrating "machines", taking information from multiple sources while making decisions that affect the final outcome. Describing and understanding the decision-making behaviour of lymphocytes within the context of th...
journal_title:Immunology and cell biology
pub_type: 杂志文章
doi:10.1038/sj.icb.7100061
更新日期:2007-06-01 00:00:00