Abstract：
:Piper methysticum (Kava) is a plant whose roots are used in the preparation of traditional beverages with spiritual, medicinal, and social importance for the Pacific Islanders. Kava is also sold as a herbal supplement or recreational beverage consumed for its mild inebriating effect in Europe and North America. With an ongoing interest in the safety and quality of kava products, it is necessary to develop a validated method for determination of kava chemical composition to ensure confidence in quality assessment. Thus, an high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was developed, optimized, and validated for determining six major kavalactones and three flavokavains in kava raw materials and finished products based on AOAC single-laboratory validation guidelines. This is the first fully validated analytical method for measuring kavalactones and flavokavains in a single run. The separation of the analytes was achieved in 10 min with an Agilent Poroshell C18 column using gradient separation. The sample was extracted with methanol first and then acetone. The signals were detected at 240 nm and 355 nm. The limit of quantification was under 1.2 µg/mL (0.3 mg/g) for kavalactones and under 0.35 µg/mL (0.01 mg/g) for flavokavains. The Horwitz ratio values described ranged from 0.3 to 1.82. The spike recovery experiments showed an accuracy between 92 and 105% for all analytes. The results of the study demonstrate that the method is fit for the purpose of determining methysticin, dihydromethysticin, kavain, dihydrokavain, yangonin, desmethoxyyangonin, flavokavain A, flavokavain B, and flavokavain C in kava raw material and finished products (dry-filled capsule, liquid phytocaps, and tincture).
journal_name
Planta Medjournal_title
Planta medicaauthors
Liu Y,Lund JA,Murch SJ,Brown PNdoi
10.1055/a-0637-2400subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1213-1218issue
16eissn
0032-0943issn
1439-0221journal_volume
84pub_type
杂志文章相关文献
PLANTA MEDICA文献大全abstract：:Lyophilised aqueous extract of Euphorbia hirta L. (Euphorbiaceae) has been evaluated for analgesic, antipyretic and anti-inflammatory properties in mice and rats, in order to complete its activity profile, after the confirmation of the existence of a central depressant activity particularly expressed by a strong sedat...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-960079
更新日期：1991-06-01 00:00:00
abstract：:Vitex agnus-castus, the chaste tree, is used against disorders of the female sexual organs and as an anaphrodisiac. Active constituents are not known. In this study the essential oil of the fruits was investigated by GC and GC-MS. ...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-957814
更新日期：1996-02-01 00:00:00
abstract：:YANG XIN is a traditional Chinese medicine formulation used for nervous fatigue and consists of a proprietary blend of concentrated extracts from 18 plant ingredients. The 18 constituent plant ingredients, YANG XIN capsules, and formulations 2014-005_1 A and 1B were extracted by consecutive 24-hour macerations with di...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0042-106170
更新日期：2016-08-01 00:00:00
abstract：:Four new phenanthroindolizidine alkaloids, tylophoridicines C-F, together with three known ones, R-(+)-deoxytylophorinidine, tylophorinine and tylophorinidine, were isolated from the roots of Tylophora atrofolliculata. The structures were determined on the basis of spectral evidence. These alkaloids exhibited cytotoxi...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2004-818973
更新日期：2004-05-01 00:00:00
abstract：:The chloroform extract of Valeriana officinalis led to the isolation of clionasterol-3-O-beta-D-glucopyranoside and a mixture of 6'-O-acyl-beta-D-glucosyl-clionasterols. The acyl moieties were identified as hexadecanoyl, 8 E,11 E-octadecadienoyl and 14-methylpentadecanoyl by alkaline hydrolysis followed by GC-MS analy...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2005-864179
更新日期：2005-10-01 00:00:00
abstract：:The culture medium of Beauveria amorpha Hohn. exhibited strong radical scavenging properties. Activity-directed fractionation assisted by a radical scavenging assay on TLC afforded two active compounds which were identified as 6-methyl-2,4-dihydroxyphenyl 4-O-methyl-beta-D-glucopyranoside (1) and (-)-terredionol (2). ...
journal_title：Planta medica
pub_type： 信件
doi：10.1055/s-2002-19868
更新日期：2002-01-01 00:00:00
abstract：:From the stem bark of Strychnos malacoclados, one new bisindole alkaloid, 3-hydroxylongicaudatine Y (1), was isolated along with the known alkaloids vomicine (2), bisnordihydrotoxiferine (3), divarine (4), longicaudatine (5), longicaudatine Y (6), and longicaudatine F (7). All the compounds were tested for their antim...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0031-1280473
更新日期：2012-03-01 00:00:00
abstract：:Ten new nervogenic acid derivatives (1-4, 6-11) and one known compound (5) have been isolated from Liparis nervosa. Their structures were determined using extensive spectroscopic analysis, including 1D and 2D NMR experiments. Compounds 3, 4, 9, 10, and 11 were evaluated for their cytotoxicity against A549, H460, Hela,...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0032-1328109
更新日期：2013-03-01 00:00:00
abstract：:The traditional therapeutic indications for the use of Morinda citrifolia L. (Rubiaceae) have been investigated. The lyophilised aqueous extract of roots of M. citrifolia was evaluated for analgesic and behavioural effects in mice. The extract did not exhibit any toxic effects but did show a significant, dose-related,...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-961004
更新日期：1990-10-01 00:00:00
abstract：:Alpinia galanga, or galangal, has been a popular condiment used in Thai and Asian cuisine for many years. However, relatively little is known of the potential beneficial or adverse health effects of this spice. This study was conducted to analyze the capacity of galangal extract to induce cytotoxicity and DNA damage i...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2007-981542
更新日期：2007-07-01 00:00:00
abstract：:The absolute stereostructure of a novel skeletal diterpene, standishinal (1), from the bark of Thuja standishii was confirmed by X-ray crystallographic analyses of 1 and its p-bromobenzoate derivative. Aromatase inhibitory activities of standishinal, eight known diterpenes, totarol, 12-methoxyabieta-8,11,13-trien-11-o...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2002-33787
更新日期：2002-08-01 00:00:00
abstract：:From the fresh rhizomes of Tamus communis five phenanthrenes (1 - 5) were isolated under the guidance of cytotoxic assays in HeLa cells. The compounds were obtained from the highly active CHCl (3) fraction of the MeOH extract by using multistep chromatographic purifications, including VLC, preparative TLC, HPLC and ge...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-941505
更新日期：2006-06-01 00:00:00
abstract：:Pancreatic cancer is one of the most aggressive human malignancies with an increasing incidence worldwide. In addition to the poor survival rates, combinations using gemcitabine as a backbone have failed to show any benefit beyond monotherapy. These facts underscore an urgent need for novel therapeutic options and mot...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0030-1249931
更新日期：2010-08-01 00:00:00
abstract：:Chemical investigation of the stems of Casearia velutina led to the isolation and structural elucidation of three new acylated glycosides, casearicosides A-C (1-3), together with 13 known compounds. The structures of the new compounds were established by spectroscopic and chemical methods. These isolates were evaluate...
journal_title：Planta medica
pub_type： 信件
doi：10.1055/s-0029-1185902
更新日期：2010-01-01 00:00:00
abstract：:Sungucine and isosungucine are two bisindole alkaloids isolated from the roots of the African plant Strychnos icaja Baillon. They both exhibit antiplasmodial activities but also show cytotoxic effects against human cancer cell lines. In order to elucidate their mechanism of action, we have investigated the interaction...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2002-32889
更新日期：2002-07-01 00:00:00
abstract：:The medicinal plants, Coutarea latiflora Sesse & Moc. ex. DC. (Hintonia latiflora Bullock) (Rubiaceae) and Exostema caribaeum (Jacq.) Roem. et Schult. (Rubiaceae) were examined for antimalarial properties. Among different crude solvent extracts of the stem bark, the hydrolysed ethyl acetate extracts were shown to have...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-960885
更新日期：1990-02-01 00:00:00
abstract：:Phytoestrogens are discussed as candidate substances to treat symptoms related to estrogen deficiency. In in vitro experiments, the naturally occurring flavonoid 6-(1,1-dimethylallyl)naringenin (6-DMAN) emerged as one of the most potent phytoestrogenic substances. 6-DMAN is not as well characterized as other flavonoid...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2004-827178
更新日期：2004-07-01 00:00:00
abstract：:Herbal medicine has been used to treat diseases for centuries; however, the biological active components and the mechanistic understanding of actions of plant-derived drugs are permanently discussed. MicroRNAs are a class of small, non-coding RNAs that play crucial roles as regulators of gene expression. In recent yea...
journal_title：Planta medica
pub_type： 杂志文章,评审
doi：10.1055/s-0042-108450
更新日期：2016-08-01 00:00:00
abstract：:Four new benzofurans trans-5-(3-hydroxypropyl)-7-methoxy-2-[2,3-dihydro-3-hydroxymethyl-7-methoxy-2-(3-methoxy-4-hydroxyphenyl)-benzofuran-5-yl]benzofuran (1), (E)-5-(2-formylvinyl)-7-methoxy-2-(3,4-methylenedioxyphenyl)benzofuran (2), 5-(3-butanoyloxypropyl)-7-methoxy-2-(3,4-methylenedioxyphenyl)benzofuran (3), and 5...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2005-871226
更新日期：2005-09-01 00:00:00
abstract：:In this study, hemolysis, Western blot, and real-time RT-PCR assays were performed to evaluate silibinin's activity against S. aureus α-toxin secretion. In addition, live/dead cell staining and lactate dehydrogenase activity assays were introduced to examine the influence of silibinin on α-toxin-induced cell injury in...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0032-1328068
更新日期：2013-01-01 00:00:00
abstract：:A cell-based method for screening drug candidates from herbal extracts that have possible anti-type II diabetic effects was established. The differentiation of preadipocytes into adipocytes was used as a sensitive primary indicator of a drug's potential effect on type II diabetes. We established a quantitative method ...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2005-916215
更新日期：2006-01-01 00:00:00
abstract：:Two new phenolic glycosides characterized as idesin salicylate (1) and 2-hydroxyphenol-1-O-beta-D-glucopyranosyl-(6-->1)- alpha-L-rhamnopyranoside (2) were isolated with four known compounds, idescarpin (3), idesin (4), 1-hydroxy-6-oxocyclohex-2-enecarboxylic acid methyl ester (5) and salirepin (6), from the fruits of...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-951771
更新日期：2007-02-01 00:00:00
abstract：:Blue cohosh, Caulophyllum thalictroides (L.) Michx. is a popular herb, roots and rhizomes of which have been extensively used for women's health. Alkaloids and saponins are considered to be responsible for its pharmacological effects. In this investigation the methanolic extract of the roots of blue cohosh, the alkalo...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0028-1112207
更新日期：2009-03-01 00:00:00
abstract：:The gamma-amino butyric acid (GABA) type A (GABA(A)) receptor represents a crucial target for clinical agents in the treatment of anxiety and insomnia. Using the two-microelectrode voltage clamp technique on recombinant α₁β₂γ(2S) GABA (A) receptors, effusol (1) and dehydroeffusol (2) were isolated in a bioactivity-gui...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0031-1298174
更新日期：2012-03-01 00:00:00
abstract：:This paper reports the phytochemical investigation of the 50% aq. EtOH extract of Houttuynia cordata, an effective TCM and functional food in China, which led to the isolation of 17 flavonoids including four new ones. The four new compounds were flavonoid derivatives tethered with houttuynin (3-oxododecanal). Each of ...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-0033-1351051
更新日期：2013-12-01 00:00:00
abstract：:Plumbagin, an active principle from Plumbaginaceous plants, produced a triphasic inotropic response (first positive, second negative, and third positive phases) in guinea-pig papillary muscle. The inotropic potency of plumbagin at the first positive phase was pD2 6.40, and the methylation of 5-hydroxy group of plumbag...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2006-960106
更新日期：1991-08-01 00:00:00
abstract：:Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles. Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an incre...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/a-0835-6622
更新日期：2019-04-01 00:00:00
abstract：:An ionpair-HPLC-technic using a reversed-phase column was developed to screen plant extracts for cardiotonic amines and to isolate them in a preparative scale for direct MS and pharmacological investigations. By this method the positive inotropic tyramine could be isolated from Selenicereus grandiflorus (L.) Britt et ...
journal_title：Planta medica
pub_type： 杂志文章
doi：10.1055/s-2007-971397
更新日期：1982-01-01 00:00:00
abstract：:Mulberroside A, a major stilbene constituent of MORUS ALBA L. (Moraceae), displays significant antitussive and antiasthmatic effects in animals. As part of our ongoing research on its biotransformation in rats, mulberroside A was orally administered to rats, and the metabolites in the gastrointestinal contents and fec...
journal_title：Planta medica
pub_type： 信件
doi：10.1055/s-0029-1186160
更新日期：2010-03-01 00:00:00
abstract：:The essential oil composition of three Zingiberaceae widely used as medicinal aromatic plants from S. Tomé and Príncipe: Aframomum danielli (Hook. f.) K. Schum., Curcuma longa L. and Zingiber officinale Rosc. was studied. Two samples of the essential oils from fruit of A. danielli and from rhizomes of the other two sp...
journal_title：Planta medica
pub_type： 信件
doi：10.1055/s-2001-16494
更新日期：2001-08-01 00:00:00
