Abstract：
:There are clinical situations in which it may be advantageous to monitor delayed type hypersensitivity (DTH) responses, an index of cell-mediated immunity, without exposing patients directly to the challenge antigens. For example, transplant patients may be at risk for becoming sensitized to donor antigens if injected with donor antigen during traditional skin tests. We describe an alternative method for human DTH testing, which involves the transfer of human peripheral blood mononuclear cells plus antigen into the pinnae or footpads of naive mice. This induces a measurable DTH-like swelling response, which we refer to as the "trans vivo DTH response." As proof of principle, we provide data obtained during trans vivo DTH studies with tetanus toxoid, cytomegalovirus (CMV) and alloantigens. In general, human T cells must be co-localized with antigen and human macrophages to produce swelling responses, and such responses are antigen-specific and require prior antigen sensitization. Not only does this assay offer a simple, reliable clinical monitoring device, but it also provides a model with which to study the in vivo mechanisms of human DTH responses.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Carrodeguas L,Orosz CG,Waldman WJ,Sedmak DD,Adams PW,VanBuskirk AMdoi
10.1016/s0198-8859(99)00002-6subject
Has Abstractpub_date
1999-08-01 00:00:00pages
640-51issue
8eissn
0198-8859issn
1879-1166pii
S0198-8859(99)00002-6journal_volume
60pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract：:A novel cell surface molecule, DN4, defined by an mAb raised against human thymic epithelial cells, showed a specificity for epithelial cells of the thymic cortex. This antigen was not expressed at detectable levels on any other types of tissues in the human body except for the thymus and bone marrow. Immunohistochemi...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(95)00168-9
更新日期：1996-02-01 00:00:00
abstract：:The eotaxin gene family (eotaxin, eotaxin-2, and eotaxin-3) has been implicated in the recruitment of eosinophils, basophiles and Th2 lymphocytes that is a central aspect of allergic diseases. We previously suggested that Eo2+179T>C and Eo2+275C>T of the eotaxin-2, and Eo3+2497T>G of the eotaxin-3 were significantly a...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2005.01.002
更新日期：2005-03-01 00:00:00
abstract：:A proinflammatory cytokine interleukin-6 (IL-6) plays an important role in the development, pathogenesis and outcome of SIRS, sepsis and septic shock. We have evaluated the role of the IL-6 gene polymorphisms in pediatric patients. A total of 421 consecutive pediatric patients admitted to the pediatric intensive care ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2007.06.003
更新日期：2007-09-01 00:00:00
abstract：:Cord blood, donated by mothers after the birth of their children, has become an accepted source of related and unrelated hematopoietic stem cells for marrow reconstitution. We estimate that some 7-8000 unrelated-donor cord blood transplants have been performed worldwide since 1993. The development of cord blood as a s...
journal_title：Human immunology
pub_type： 杂志文章,评审
doi：10.1016/j.humimm.2006.03.015
更新日期：2006-06-01 00:00:00
abstract：:Little is known about the etiologies of diseases associated with circulating antineutrophil cytoplasm autoantibodies (ANCA), such as primary vasculitides and inflammatory bowel diseases. However, the understanding of immune mechanisms supposedly involved in the pathogenesis of these diseases is still growing. In the p...
journal_title：Human immunology
pub_type： 杂志文章,评审
doi：10.1016/j.humimm.2003.09.013
更新日期：2004-01-01 00:00:00
abstract：:Screening of a human cosmid library representing genomic DNA from an individual homozygous for the HLA-DR2 B7 A2 haplotype yielded 109 class I HLA-specific clones. One cosmid clone, Ice 6.23, had a full-length nonclassical class I gene within a 5.4-kb HindIII fragment. The Ice 6.23-5.4H gene was cloned into the unique...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(91)90106-j
更新日期：1991-10-01 00:00:00
abstract：:We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in 1217 Mexicans from the Mexico City Metropolitan Area living in the northern (N = 751), southern (N = 52), eastern (N = 79), western (N = 33), and central (N = 152) Mexico City, and rural communities (N = 150), to obtai...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2019.07.297
更新日期：2020-09-01 00:00:00
abstract：:In our earlier studies, single nucleotide polymorphisms (SNPs) associated with anti-inflammatory cytokines were found to influence risk for breast cancer in western Indian women. Analysis of Interleukin 6 (IL-6) -174G>C polymorphism in this cohort (patients = 182; controls = 236) suggested a protective role for IL-6 -...
journal_title：Human immunology
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.humimm.2014.06.018
更新日期：2014-08-01 00:00:00
abstract：:A panel of 34 clones was established from a cell line derived from the skin biopsy of a patient (genotype: A1, A2, B7, B8, DR3, DR6) undergoing acute graft-versus-host disease after semiallogeneic bone marrow transplantation with his mother's bone marrow (genotype: A1, A1, B7, B8, DR3, DR6). The T-cell line obtained p...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(90)90074-y
更新日期：1990-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to determine whether the SUMO4 M55V (A163G) polymorphism confers susceptibility to type 1 diabetes (T1D). METHODS:A meta-analysis was conducted on the association between the SUMO4 M55V polymorphism and T1D using; (1) allelic contrast (G vs. A), and the (2) recessive (GG vs. GA+AA),...
journal_title：Human immunology
pub_type： 杂志文章,meta分析
doi：10.1016/j.humimm.2012.07.341
更新日期：2012-10-01 00:00:00
abstract：:This study was undertaken to determine the prevalence of dengue clinical symptom persistence during 60days of disease follow up, in patients of Espírito Santo state (ES)-Brazil and to evaluate the relation of single nucleotide polymorphisms (SNPs) in FcγRIIa, CD209, VDR, TNF-α, IL-4, IL-6 and IFN-γ genes with symptom ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2015.09.026
更新日期：2015-10-01 00:00:00
abstract：:Human T suppressor cells (Ts), capable of preventing autologous T helper cells (Th) from reacting against xenogeneic pig endothelial cells and pig APC can be generated in vitro. Ts derive from a population of CD3(+)CD8(+)CD28(-) T lymphocytes and specifically recognize the MHC class I antigens of the APC used for in v...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(01)00238-5
更新日期：2001-05-01 00:00:00
abstract：:CTLA-4 inhibitory molecule plays an important role in regulating T cell activation. It is considered a crucial element in keeping the immune balance and has been implicated in cancer, autoimmunity and transplantation immunology. Inconsistent observations are reported regarding its association with hematopoietic stem c...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2013.08.002
更新日期：2013-12-01 00:00:00
abstract：:One of the major categories of disease-causing micro-organisms are viruses. New studies on many different viruses have shown that virus attachment and cell entry is often a multistep process, requiring many interactions between the virus and cell surface molecules. In this study, we have attempted to identify the cell...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(00)00151-8
更新日期：2000-09-01 00:00:00
abstract：:Recent studies have suggested a clinical significance to the detection of anti-major histocompatibility complex class I-related chain A (MICA) antibodies in transplantation. We have developed an eplet-based version of the HLAMatchmaker algorithm to assess the epitope specificity of these antibodies. Molecular viewing ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2008.10.003
更新日期：2008-12-01 00:00:00
abstract：:This review focuses on the role of antigen-specific T cells that mediate active inhibition of immune responses over the past 35 years since their initial description. The field has experienced several changes in the accepted paradigm of such suppressor/regulatory T cells, from initial indications that such cells were ...
journal_title：Human immunology
pub_type： 杂志文章,评审
doi：10.1016/j.humimm.2008.07.018
更新日期：2008-11-01 00:00:00
abstract：:Rat soluble MHC class I synthesis was studied at both RNA and protein levels to determine whether multiple forms of soluble MHC class I molecules are produced by different mechanisms. RT-PCR and sequencing of MHC class I transcripts identified an alternatively spliced nonclassical MHC class I gene product, lacking bot...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(98)00039-1
更新日期：1998-07-01 00:00:00
abstract：UNLABELLED:Early allograft dysfunction (EAD) after liver transplantation is mostly a reversible event caused by factors related to ischemia/reperfusion (I/R) injury. EAD represents a hepatic injury associated with pre- and early post-transplant inflammatory cytokine responses. Aim of the present study was to evaluate t...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2016.07.234
更新日期：2016-10-01 00:00:00
abstract：:Killer immunoglobulin-like receptors (KIRs) regulate function of NK cells and subsets of T cells. HLA class I molecules are ligands for inhibitory KIRs while specificity of activating KIRs is mainly unknown. Both KIR and HLA genotypes are highly polymorphic. In this study we analyzed associations of KIR and KIR ligand...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2014.04.002
更新日期：2014-06-01 00:00:00
abstract：:The complement system (CS) is a key element of immunity against pathogens but also seems to influence other events, such as tumorigenesis and tissue repair. Complement component 7 (C7) is a key component of the lytic pathway of CS, leading to the formation of the membrane attack complex (MAC). This study aimed to inve...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2018.06.009
更新日期：2018-09-01 00:00:00
abstract：:The susceptibility to type 2 diabetes (T2D) involves genetic factors. We studied the distribution of KIR and MHC class I ligands phenotype and genotype frequencies, as well as immunoglobulin KM and GM allotype frequencies in a group of patients (N = 95) with T2D and ethnically matched healthy controls (N = 74) with Pu...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2006.08.297
更新日期：2006-11-01 00:00:00
abstract：:The purpose of this article is to study the amounts of gamma-aminobutyric acid-transaminase (GABA-t) during immune response in the human thymus. GABA-t was studied by biochemical and histochemical methods in 7 immunostimulated (treated) and 7 non-immunostimulated (untreated) patients (4 young adult, age range: 24-36 y...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(00)00130-0
更新日期：2000-07-01 00:00:00
abstract：:Dendritic cells play an important role in the initiation of immune reactions. Due to their high capacity to prime T-cell responses, the activation of dendritic cells must be tightly controlled. Because Interleukin-1β (IL-1β) is a key player in autoinflammatory diseases, we compared the ability of IL-1β to activate hum...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2018.06.002
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVE:The aims of this study were to identify the candidate causal single nucleotide polymorphisms (SNPs) and candidate causal mechanisms of asthma and to generate SNP to gene to pathway hypotheses. METHODS:SNPs that met a threshold of p ≤ 0.001 in a genome-wide association study (GWAS) dataset of asthma, which in...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2012.11.003
更新日期：2013-02-01 00:00:00
abstract：:DC-10 is a distinct subset of human tolerogenic dendritic cells (DCs) which express high levels of human leukocyte antigen-G (HLA-G). DC-10 could induce adaptive type 1 regulatory T cells through the IL-10 dependent ILT4/HLA-G signaling pathway. However, the significance of DC-10 in malignancies remains unclear. In th...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2016.01.003
更新日期：2016-09-01 00:00:00
abstract：:To commence proper antibiotic treatment in sepsis, timely knowledge of whether the cause of systemic infection is gram-negative (gram(-)) or gram-positive (gram(+)) bacteria in origin would be beneficial for clinicians. In this clinical prospective study, our objective was to develop a method for distinguishing betwee...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2009.01.009
更新日期：2009-04-01 00:00:00
abstract：:To simplify PCR-SSO HLA-DRB generic typing, we labeled eight of 15 oligonucleotide probes with DIG and the others with biotin, and hybridized each dot blot with both a biotin-labeled probe and a DIG-labeled probe simultaneously. We chose oligonucleotide pairs which require the same hybridization and stringent washing ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(94)90097-3
更新日期：1994-01-01 00:00:00
abstract：:A large percentage of healthy individuals (50-90%) is chronically infected with Cytomegalovirus (CMV). Over the past few years, several techniques were developed in order to monitor CMV-specific T-cell responses. In addition to the identification of antigen-specific T cells with peptide-loaded MHC complexes, most of t...
journal_title：Human immunology
pub_type： 杂志文章,评审
doi：10.1016/j.humimm.2004.02.012
更新日期：2004-05-01 00:00:00
abstract：:To clarify the molecular relationship between HLA loci and ulcerative colitis (UC) in Japanese patients, we performed HLA-DP genotyping by the PCR-RFLP method and studied tumor necrosis factor beta-chain genetic polymorphism by Southern hybridization, in addition to conventional serologic typing. Significant increase ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(93)90113-f
更新日期：1993-02-01 00:00:00
abstract：:The intriguing observation made by Geczy et al. (1) showing the possibility of generating specific ankylosing spondylitis--cytotoxic T lymphocytes by presenting HLA-B27+AS+ cells as antigen-specific stimulator cells prompted us (by using Geczy's approach) to identify cytotoxic T lymphocytes specific for this apparent ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(89)90078-5
更新日期：1989-07-01 00:00:00
