Abstract:
:To simplify PCR-SSO HLA-DRB generic typing, we labeled eight of 15 oligonucleotide probes with DIG and the others with biotin, and hybridized each dot blot with both a biotin-labeled probe and a DIG-labeled probe simultaneously. We chose oligonucleotide pairs which require the same hybridization and stringent washing conditions and do not compete with each other during hybridization. After incubation with a mixture of anti-DIG Fab fragment-alkaline phosphatase and streptavidin-peroxidase conjugates, specific binding of the DIG-labeled probe was revealed by a chemiluminescent substrate (CSPD) and specific binding of the biotin-labeled probe was subsequently visualized by a chromogenic substrate (TMB). The sensitivity of both probes was similar and gave comparable hybridization signals. Using this simplified procedure, the number of hybridizations or dot blots can be reduced to half the usual amount and the labor involved in PCR-SSO typing significantly reduced.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Chen DF,Endres W,Meyer SA,Stangel Wdoi
10.1016/0198-8859(94)90097-3subject
Has Abstractpub_date
1994-01-01 00:00:00pages
25-30issue
1eissn
0198-8859issn
1879-1166pii
0198-8859(94)90097-3journal_volume
39pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract::The purpose of this article is to study the amounts of gamma-aminobutyric acid-transaminase (GABA-t) during immune response in the human thymus. GABA-t was studied by biochemical and histochemical methods in 7 immunostimulated (treated) and 7 non-immunostimulated (untreated) patients (4 young adult, age range: 24-36 y...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00130-0
更新日期:2000-07-01 00:00:00
abstract::The chemokine C receptor 7 (CCR7) is a G-protein-coupled heptahelical receptor (GPCR) that is expressed on a wide variety of cells including memory T cells, B cells, mature dendritic cells, and cancer cells. Activated by its ligands CCL19 or CCL21, CCR7 plays a major role in metastasis of cancer cells. Recent studies ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2015.03.010
更新日期:2015-05-01 00:00:00
abstract::A proinflammatory cytokine interleukin-6 (IL-6) plays an important role in the development, pathogenesis and outcome of SIRS, sepsis and septic shock. We have evaluated the role of the IL-6 gene polymorphisms in pediatric patients. A total of 421 consecutive pediatric patients admitted to the pediatric intensive care ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2007.06.003
更新日期:2007-09-01 00:00:00
abstract::While modern high-throughput sequence-based HLA genotyping methods generally provide highly accurate typing results, artefacts may nonetheless arise for numerous reasons, such as sample contamination, sequencing errors, read misalignments, or PCR amplification biases. To help detecting spurious typing results, we test...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.01.012
更新日期:2016-03-01 00:00:00
abstract::Virus-specific T cells have been shown to cross-react with allogeneic HLA (allo-HLA) at a clonal level. However, the impact of a single virus on the allorepertoire has never been investigated at the polyclonal level. We made an inventory of the incidence and specificity of allo-HLA-cross-reactive-virus-specific CD8+ T...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2018.10.014
更新日期:2019-02-01 00:00:00
abstract::Felty's syndrome (FS) is a rare complication of rheumatoid arthritis (RA) previously shown to be strongly associated with HLA-DR4 and less significantly with HLA-DQw7. To map more precisely the HLA locus responsible for susceptibility to FS, we have examined HLA-DR4 and DQ beta-chain polymorphisms in FS patients and c...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90117-r
更新日期:1991-09-01 00:00:00
abstract::Alloantibodies are known to influence transplant outcomes. Apart from human leukocyte antigens (HLA), non-HLA targets have been suggested to play a significant role, but little is known about their nature. Here, we present a novel method for identification and characterization of cell surface antigens bound by allorea...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2010.02.006
更新日期:2010-05-01 00:00:00
abstract::Juvenile dermatomyositis (JDMS) is an inflammatory disease associated with HLA-DR3. We therefore undertook molecular genetic studies of HLA region genes to determine whether HLA-DR3 itself confers susceptibility to JDMS or whether susceptibility is conferred by alleles in linkage disequilibrium with HLA-DR3. Our resul...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90085-n
更新日期:1991-12-01 00:00:00
abstract::The polymorphic nature of the KIR3DL1/S1 gene complex has been investigated through the development of a polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) allele typing system. The KIR3DL1/S1 system was applied to a healthy group of Northern Irish individuals to establish allele frequencies...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2004.03.003
更新日期:2004-06-01 00:00:00
abstract::The aim of this study was to examine the role of single nucleotide polymorphisms (SNPs) and haplotypes of the tumor necrosis factor ligand superfamily member 15 (TNFSF15) gene in Hungarians with psoriasis and psoriatic arthritis. A case-control study was performed, and five TNFSF15 SNPs (rs3810936, rs6478108, rs647810...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.11.006
更新日期:2014-02-01 00:00:00
abstract::Rheumatoid arthritis is associated with HLA-DR4 in several ethnic groups. Since DR4 haplotypes encode a diverse array of class II molecules, it is of interest to characterize the nature of the primary association. We have examined molecular polymorphisms of HLA class II gene products expressed by normals and rheumatoi...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(89)90033-5
更新日期:1989-09-01 00:00:00
abstract::The reactivity of monoclonal antibodies (mAbs) R1, S1, and S5, shown previously to recognize polymorphic epitopes on HLA-DQ molecules, have been found to correlate with the presence of certain DQB1 alleles. mAb S5 reacts with cells expressing DQB1*0503, 0601, 0602, 0603, or 0604 alleles while R1 and S1 react with all ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90009-x
更新日期:1991-06-01 00:00:00
abstract::The association of Ig allotypes with celiac disease was analyzed in a sample of 95 Italian patients typed for HLA polymorphisms. No significant association was found for any Gm, A2m, or Km specificity when the overall sample was considered. However, significant different sex ratios were shown by patients' fnb positive...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(83)90065-4
更新日期:1983-02-01 00:00:00
abstract::The multidrug resistance gene (mdr1) is a member of the recently described ATP binding cassette (ABC) superfamily of transporters. Family members include: (1) the cystic fibrosis transmembrane conductance regulator gene; (2) the hlyB gene of bacteria, and (3) the histocompatibility antigen modifier (HAM) gene. The lev...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90110-u
更新日期:1991-10-01 00:00:00
abstract::The purpose of this paper is to report a pedigree with hereditary hemochromatosis in which a recombination between the HLA-A and B loci occurred. Both the maternal and paternal HLA-A3, B7 haplotypes were carrying an allele for hemochromatosis. The A3, B12 haplotype of the proband was a recombinant of the maternal A3, ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(80)90005-1
更新日期:1980-07-01 00:00:00
abstract::The purpose of this study was to identify polymorphic residues of DQ2 beta chains which are involved in the epitopes recognized by DQ2-reactive mAbs. Binding studies were performed on a panel of DQ2 mutant cells made by transfection of a DQ (alpha 1*0501, beta 1*0301)-positive B-LCL with DQB1 genes encoding either wil...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(95)00047-8
更新日期:1995-10-01 00:00:00
abstract::The association of HLA antigens and type I or "lupoid" CAH-C was investigated in a population of 52 Argentinian Caucasoid patients. When compared with a population of normal individuals of the same ethnic group (n = 197), a significant increase of HLA-DR6 was observed (68.6% in patients vs 17.3% in controls; RR = 12.3...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)90008-6
更新日期:1994-10-01 00:00:00
abstract::There is increased evidence about the deleterious effect of delayed graft function (DGF) in both short- and long-term kidney graft outcome. Among the mechanisms involved in the production of DGF, immune factors play a role, especially in the level of hypersensitization. From the 1389 patients transplanted at our hospi...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2005.01.026
更新日期:2005-04-01 00:00:00
abstract::To clarify the molecular relationship between HLA loci and ulcerative colitis (UC) in Japanese patients, we performed HLA-DP genotyping by the PCR-RFLP method and studied tumor necrosis factor beta-chain genetic polymorphism by Southern hybridization, in addition to conventional serologic typing. Significant increase ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(93)90113-f
更新日期:1993-02-01 00:00:00
abstract::The metalloproteinase (MPase)-mediated pathway of MHC class I processing is a distinct cellular mechanism that generates soluble HLA proteins. It has been implicated in modulation of immune responses induced during transplantation events. It is, therefore, important to define the characteristics of soluble HLA species...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00213-5
更新日期:2000-12-01 00:00:00
abstract::Human leukocyte antigen-G (HLA-G) is a nonclassical tolerogenic molecule that can be expressed either as membrane bound (HLA-G1) or secreted (HLA-G5) isoforms. Upregulation of HLA-G1 or HLA-G5 expression by tumor cells constitutes an efficient way to escape from antitumoral immune responses. The inhibitory role of HLA...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.07.019
更新日期:2009-12-01 00:00:00
abstract::It has been extensively documented the role of the indirect pathway of allorecognition in allograft rejection. However, recent data demonstrate that the manipulation of this pathway could be also sufficient to promote prolongation of allograft survival. In the present study we evaluated the effect of preoperative immu...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(02)00451-2
更新日期:2002-10-01 00:00:00
abstract::MUC1 mucin, primarily known as an epithelial antigen, has been demonstrated to be expressed on activated human T cells. In the present study, we first examined the expression of MUC1 on different subsets of T cells (naive, effector, effector/memory). MUC1 appears to be strongly upregulated on activated CD4(+) T cells ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2012.02.024
更新日期:2012-05-01 00:00:00
abstract::The aim of this study was to assess the possible association of the functional (GT)(n) microsatellite polymorphism in the FOXP3 gene with predisposition to several autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), ulcerative colitis (UC), Crohn's disease, and celiac disease. We...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2005.06.001
更新日期:2005-08-01 00:00:00
abstract::Previously, most models that sought to explain the misregulation of immune cell function assumed molecular similarities between the disease-causing pathogens and the host's proteins. In recent time several different models have been proposed and in this study, these concepts are compared to a new hypothesis proposing ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.05.010
更新日期:2009-09-01 00:00:00
abstract::The loss of melanocytes in vitiligo is mainly attributed to defective autoimmune mechanisms and lately autoinflammatory mediators have become more emphasized. Among these, a number of class II cytokines and their receptors have displayed altered expression patterns in vitiligo. Thus, we selected 30 SNPs from the regio...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2015.09.050
更新日期:2016-05-01 00:00:00
abstract::The co-stimulatory molecule CD28 plays an important role in T-cell-mediated immune response like acute cellular liver transplant rejection. The aim of the retrospective case- control study was to examine whether the single nucleotide polymorphisms (SNPs) rs3116487, rs3116494, and rs3116496 of the CD28 gene are associa...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2020.10.002
更新日期:2020-12-01 00:00:00
abstract::We have investigated if recombinant granulocyte-macrophage colony stimulating factor (GM-CSF), alone or in concert with recombinant gamma interferon, affects the endothelial cell expression of class I major histocompatibility complex antigen. Results obtained show that the GM-CSF increases class I expression on the en...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(90)90016-i
更新日期:1990-06-01 00:00:00
abstract::The purpose of this study was to examine the expression of human leukocyte antigen-G (HLA-G) in patients with systemic lupus erythematosus (SLE) and its relation with interleukin-10 (IL-10) production. The study included 50 female SLE patients and 59 healthy female donors. HLA-G expression in peripheral blood and cuta...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2007.11.001
更新日期:2008-01-01 00:00:00
abstract::We compared the immunohistochemical reaction patterns of HLA-G-specific antibodies 87G, 4H84, G233, 16G1, and BFL.1 on human placentas under three different preparative conditions and on cryosections of other human tissues. Human and murine cell lines, either naturally expressing or transfected with HLA-G, were analyz...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00207-x
更新日期:2000-11-01 00:00:00