Abstract：
:Screening of a human cosmid library representing genomic DNA from an individual homozygous for the HLA-DR2 B7 A2 haplotype yielded 109 class I HLA-specific clones. One cosmid clone, Ice 6.23, had a full-length nonclassical class I gene within a 5.4-kb HindIII fragment. The Ice 6.23-5.4H gene was cloned into the unique NotI site of an expression vector pSV2.Not, a derivative of pSV2neo, which was constructed to contain a second SV40 early region promoter adjacent to an introduced NotI site. The resulting construct was transfected into the P815-B2M cell line, a derivative of the mouse mastocytoma P815 (HTR) line which expressed human beta2-microglobulin following stable transfection with a cloned human beta2-microglobulin gene. Following transfection the Ice 6.23-5.4 H gene was found to be expressed at both the mRNA and cell surface product levels. DNA sequencing of this gene suggests that it is allelic to the HLA-6.0 gene clone (HLA-G) of Geraghty et al. (Proceedings of the National Academy of Sciences USA, 84:9145, 1987); thereby revealing a HindIII restriction fragment length polymorphism at the HLA-G locus. An extraordinarily high degree of sequence similarity (99.92%) between these two genes, which derive from unrelated HLA haplotypes, suggests strong conservative selection pressure at the HLA-G locus. A flanking single copy sequence probe 4 kb distant from the Ice 6.23-5.4H gene was used to generate long-range restriction mapping at the HLA-G locus.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Pook MA,Woodcock V,Tassabehji M,Campbell RD,Summers CW,Taylor M,Strachan Tdoi
10.1016/0198-8859(91)90106-jsubject
Has Abstractpub_date
1991-10-01 00:00:00pages
102-9issue
2eissn
0198-8859issn
1879-1166pii
0198-8859(91)90106-Jjournal_volume
32pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract：:The aim of this study was to determine the prognostic significance of soluble human leukocyte antigen (HLA) class I (sHLA-I) and HLA-G molecules in lung cancer patients. A total of 23 small-cell lung cancer (SCLC) and 114 non-small-cell lung cancer (NSCLC) patients, including 55 adenocarcinoma, 46 squamous cell carcin...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2010.02.015
更新日期：2010-05-01 00:00:00
abstract：:Conventional matching is based on numbers of alleles shared between donor and recipient. This approach, however, ignores the degree of relationship between alleles and haplotypes, and therefore the actual degree of difference. To address this problem, we have compared family members using a block matching technique wh...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(97)00231-0
更新日期：1998-01-01 00:00:00
abstract：:In the present study, we explored the specific requirements for lysis of human activated T cells by CD4+ CTLs. This was achieved by using human CD4+ T cell lines or clones specific for a peptidic fragment of influenza virus as both CTL effectors and target T cells (TTCs). Our results further establish that human activ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(92)90094-4
更新日期：1992-09-01 00:00:00
abstract：:The monoclonal antibodies (MoAbs) KS3 and KS4 are secreted by hybridomas constructed with splenocytes from a BALB/c mouse sequentially immunized with the cultured lymphoid cells JKu and LG-2 which share only the HLA-B27 specificity. Serologic and immunochemical assays have shown that the two MoAbs recognize the same (...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(88)90071-7
更新日期：1988-03-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) infection has been associated with enhanced expression of HLA antigens on the endothelium and with cellular infiltrates within the graft following human organ transplantation. We investigated the interactions between human cytomegalovirus-infected cultured endothelial cells and cocultured ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(95)00095-x
更新日期：1995-11-01 00:00:00
abstract：:The aim of this work was to investigate the longitudinal evolution of plasmatic soluble HLA-G (sHLA-G: shed HLA-G1 plus HLA-G5) during pregnancy, and if peripheral maternal antigen presenting cells (APC) can be a source of sHLA-G. Blood samples were obtained from 45 volunteers during normal pregnancy, 8 of them monthl...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2007.04.007
更新日期：2007-08-01 00:00:00
abstract：:4-Hydroxynonenal (HNE) is the most abundant and toxic aldehyde generated by the oxidation of plasma membrane polyunsaturated fatty acids. Systemic lupus erythematosus (SLE), a chronic autoimmune disease, is primarily characterized by increased levels of autoantibodies, predominantly against ds-DNA. However, the initia...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2012.08.011
更新日期：2012-11-01 00:00:00
abstract：:In attempts to examine the relationships between serologic and structural polymorphisms of HLA-DQ molecules we have analyzed several monoclonal antibodies generated against polymorphic determinants on HLA-DQ molecules. One antibody, SFR20-DQw3, has a serologic reactivity like that of the previously characterized anti-...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(87)90076-0
更新日期：1987-04-01 00:00:00
abstract：:Previous studies indicate the distribution of major histocompatibility complex class I chain-related genes A (MICA) and B (MICB) alleles and haplotypes varies widely between different ethnic populations and geographic areas. It is meaningful to investigate allelic frequencies and establish a genetic database. In this ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2016.03.005
更新日期：2016-05-01 00:00:00
abstract：:Recent studies have suggested a clinical significance to the detection of anti-major histocompatibility complex class I-related chain A (MICA) antibodies in transplantation. We have developed an eplet-based version of the HLAMatchmaker algorithm to assess the epitope specificity of these antibodies. Molecular viewing ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2008.10.003
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND:X-linked lymphoproliferative disease (XLP) is a rare life-threatening syndrome. Rapid recognition and definitive diagnosis are critical to improve the prognosis and survival of patients with XLP. Nowadays, little is known about patients with XLP in China. METHODS:We report the characterization of five Chine...
journal_title：Human immunology
pub_type： 杂志文章,评审
doi：10.1016/j.humimm.2016.06.005
更新日期：2016-08-01 00:00:00
abstract：:A PCR-based SSO-assay has been developed to characterize the allelic polymorphism at the HLA-DPA1 locus. To validate the performance of this assay, 77 samples were typed side by side in a blinded fashion by the SSO assay and sequencing-based typing (SBT); 100% concordance was seen between the two methods. To address q...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(97)00208-5
更新日期：1997-12-01 00:00:00
abstract：:The purpose of this paper is to report a pedigree with hereditary hemochromatosis in which a recombination between the HLA-A and B loci occurred. Both the maternal and paternal HLA-A3, B7 haplotypes were carrying an allele for hemochromatosis. The A3, B12 haplotype of the proband was a recombinant of the maternal A3, ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(80)90005-1
更新日期：1980-07-01 00:00:00
abstract：:The presence of complement fixing anti-human leukocyte antigen (HLA) antibodies in the circulation of organ transplant recipients may result in heart allograft rejection. Here, we assessed the clinical impact of pre- and post-transplantation allosensitization on long-term survival of heart allografts. Sequential sampl...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2009.06.015
更新日期：2009-10-01 00:00:00
abstract：:The expression pattern of several genes associated with different processes in melanocytes, including melanogenesis, is changed in vitiligo patients. We evaluated possible changes in the expression of interleukin (IL)-10 family cytokines (IL26, IL-28A, IL28B, IL29), their receptor subunits (IL20RB, IL22RA2, IL28RA), a...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2012.01.011
更新日期：2012-04-01 00:00:00
abstract：UNLABELLED:Vesicoureteral reflux (VUR) is a common childhood problem, causing renal wounds and escalating the risk of renal deficiency and hypertension. A vast literature exists suggesting that genetic variations play a significant role in the pathogenesis of VUR. The aim of the present study was to estimate whether ge...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2012.09.005
更新日期：2013-01-01 00:00:00
abstract：:The occurrence of graft rejection episodes after orthotopic liver transplantation (OLT) despite the use of immunosuppressive drugs designed to suppress T lymphocyte functions, indicates the involvement of other types of cells in this process. The activity of natural killer cells and their killer immunoglobulin-like re...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2005.10.016
更新日期：2005-11-01 00:00:00
abstract：:Childhood B-cell precursor acute lymphoblastic leukemia (BCP ALL) is usually initiated in utero and is thought to progress to overt leukemia under the influence of delayed exposure to a common infection. Based on the hypothesis that polymorphic HLA-DP variants can restrict T-cell responses to infection, we previously ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2011.05.011
更新日期：2011-10-01 00:00:00
abstract：:Despite advances in HLA matching, graft failure remains a serious complication after allogeneic hematopoietic cell transplantation (HCT). Pre-formed anti-HLA donor-specific antibodies (DSAs) have been associated with graft failure, but the impact of newly developing (de novo) anti-HLA antibodies on HCT outcomes remain...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2018.08.009
更新日期：2018-12-01 00:00:00
abstract：:Several complement regulatory proteins exist on self-cells to prevent damage by the serum complement system. In the present study, we aimed to perform quantitative analysis of membrane-bound complement regulators, CR1 (CD35), MCP (CD46), DAF (CD55), and MIRL (CD59), on peripheral blood neutrophils, monocytes, and lymp...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2013.01.011
更新日期：2013-05-01 00:00:00
abstract：:Comparison of human leukocyte antigen (HLA) frequencies in patients with hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC) and in patients with HCV-associated non-Hodgkin's lymphoma (NHL) has not been addressed previously. To this aim, we investigated the distribution of HLA class II alleles in two sel...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2004.08.183
更新日期：2004-11-01 00:00:00
abstract：:Despite the large local donor registries, for some Israeli patients an HLA-matched unrelated donor cannot be identified within a favorable time period. This study aims to retrospectively analyze donor search processes in our transplantation center in order to provide a basis to enhance the efficacy of these procedures...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2013.04.012
更新日期：2013-08-01 00:00:00
abstract：:The association between single-nucleotide polymorphisms -174G/C (rs1800795) and -572G/C (rs1800796) in the interleukin-6 (IL-6) gene promoter region and ischemic heart disease (IHD)/ischemic stroke (IS) remains controversial and ambiguous. In this study, we performed a more precise estimation of the relationship by a ...
journal_title：Human immunology
pub_type： 杂志文章,meta分析
doi：10.1016/j.humimm.2011.03.019
更新日期：2011-08-01 00:00:00
abstract：:The HLA-A, HLA-B and HLA-C molecules have turned out to be highly polymorphic and functionally complex. They not only serve as peptide receptors, but also interact with beta 2-microglobulin, an alpha beta T cell receptor, CD8 and NK inhibitory molecules, all at different sites. The fact that more than 300 class I alle...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(97)00175-4
更新日期：1997-09-15 00:00:00
abstract：:The cytotoxic T-lymphocyte antigen 4 (CTLA4, CD152) gene is a positional and functional candidate gene to susceptibility to systemic lupus erythematosus (SLE) because CTLA4 gene maps in the described SLE risk region 2q33 and CTLA4 molecule has an inhibitory effect on T-cell activation. Several polymorphisms have been ...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/s0198-8859(03)00171-x
更新日期：2003-10-01 00:00:00
abstract：:Human leukocyte antigen G (HLA-G) molecules are crucial for the maternal tolerance against the fetus during pregnancy. Thus, the presence of soluble HLA-G (sHLA-G) in embryo cultures is thought to be correlated to a successful pregnancy after assisted reproductive techniques (ART). Here, we established a rapid detecti...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2006.11.003
更新日期：2007-04-01 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) in the T-cell receptor (TCR) gene segments might play a role in shaping the TCR repertoire. Three polymorphisms have been described for the TCRBV20S1 gene segment, one of which is responsible for a nucleotide substitution at position 524, resulting in the introduction of a stop c...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2007.01.011
更新日期：2007-05-01 00:00:00
abstract：:CD4+ T cells were cultured from posttransplant cardiac biopsies placed on irradiated feeder cells of autologous cloned donor major histocompatibility complex class II-specific T-cell lines cultured and grown from previous biopsies. Fourteen of the CD4+ T-cell cultures were expanded and cloned using the same feeder cel...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/0198-8859(90)90011-d
更新日期：1990-06-01 00:00:00
abstract：:HLA-G molecule has considerable impact in various clinical fields, therefore many studies attempted to predict its expression based on HLA-G genotype. These studies have focused on polymorphisms in either the coding region or in one of the two untranslated regions (UTR) of the gene. The aim of our study was to determi...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2012.10.027
更新日期：2013-02-01 00:00:00
abstract：:The complement system (CS) is a key element of immunity against pathogens but also seems to influence other events, such as tumorigenesis and tissue repair. Complement component 7 (C7) is a key component of the lytic pathway of CS, leading to the formation of the membrane attack complex (MAC). This study aimed to inve...
journal_title：Human immunology
pub_type： 杂志文章
doi：10.1016/j.humimm.2018.06.009
更新日期：2018-09-01 00:00:00
