当前位置： SCI文献检索 > HUMAN IMMUNOLOGY期刊下所有文献 > Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro.

Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro.

Abstract：

:Rat soluble MHC class I synthesis was studied at both RNA and protein levels to determine whether multiple forms of soluble MHC class I molecules are produced by different mechanisms. RT-PCR and sequencing of MHC class I transcripts identified an alternatively spliced nonclassical MHC class I gene product, lacking both exon 5 and 6, in both spleen and liver. Immunoprecipitation and SDS-PAGE identified two distinct soluble MHC class I proteins in both splenocyte- and hepatocyte-culture supernatants. The 36Kd classical soluble MHC class I protein (RT1.Aa) was precipitated by both allele-specific (MN4.91.6, R3/13, R2/15S) and pan-reactive (OX18) mAbs. The 39Kd non-RT1.A soluble MHC class I protein was precipitated only by OX18. The production of soluble RT1.Aa was inhibited by a metalloproteinase inhibitor, but not by serine/thiol protease inhibitors. None of these protease inhibitors interfered with the soluble non-RT1.A production, suggesting that it might be derived from an alternatively spliced MHC class I transcript. The soluble non-RT1.A was always associated with beta2m. However, soluble RT1.Aa molecule was cleaved in beta2m-free form and was reassociated with beta2m in culture supernatants. Thus two soluble MHC class I molecules, classical (36Kd RT1.Aa) and nonclassical (the alternatively spliced transcript), were produced from rat cells. Alternative splicing led to the nonclassical soluble MHC class I synthesis. Proteolytic cleavage by metalloproteinase led to the classical soluble MHC class I synthesis.

journal_name

Hum Immunol

journal_title

Human immunology

authors

Zhai Y,Knechtle S

doi

10.1016/s0198-8859(98)00039-1

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

404-14

issue

7

eissn

0198-8859

issn

1879-1166

pii

S0198885998000391

journal_volume

59

pub_type

杂志文章

相关文献

HUMAN IMMUNOLOGY文献大全
  • Streptococcus pyogenes type 5 M protein is an antigen, not a superantigen, for human T cells.

    abstract：:M proteins are coiled-coil dimers expressed on group A streptococcal cell surfaces. They have an important role in host antistreptococcal immunity and in poststreptococcal autoimmune sequelae. Controversy has arisen regarding whether type 5 M proteins are superantigenic for human T cells. To investigate this, we have ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/S0198-8859(97)00028-1

    authors： Degnan B,Taylor J,Hawkes C,O'Shea U,Smith J,Robinson JH,Kehoe MA,Boylston A,Goodacre JA

    更新日期：1997-04-01 00:00:00

  • Molecular complexity of HLA-DQw3: the TA10 determinant is located on a subset of DQw3 beta chains.

    abstract：:In attempts to examine the relationships between serologic and structural polymorphisms of HLA-DQ molecules we have analyzed several monoclonal antibodies generated against polymorphic determinants on HLA-DQ molecules. One antibody, SFR20-DQw3, has a serologic reactivity like that of the previously characterized anti-...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(87)90076-0

    authors： Radka SF,Scott RG,Stewart SJ

    更新日期：1987-04-01 00:00:00

  • Cytofluorometric analysis of major histocompatibility antigens on human monocytes using monoclonal antibodies.

    abstract：:The expression of HLA-A, B, C, and DR antigens was investigated on monocyte preparations by flow cytometry using various monoclonal antibodies. Essentially all human monocytes, either freshly isolated or after culture for several days, were stained for HLA-A, B, C, and DR antigens. When monocytes were incubated with C...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(83)90069-1

    authors： Nunez G,Stastny P

    更新日期：1983-01-01 00:00:00

  • Lack of shared lymphocyte-stimulating determinants between H-2I and HLA-D/DR using mouse primed lymphocyte typing cells.

    abstract：:A number of anti-H-2 alloantisera containing antibody reactive with I region gene products (Ia) of the major histocompatibility complex cross-react with determinants expressed by human peripheral blood B lymphocytes. Such data have led to the conclusion that Ia and DR antigens share cross-reacting determinants. We hav...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(83)80011-1

    authors： Shaut DM,Ireland J,Scornik JC,Peck AB

    更新日期：1983-06-01 00:00:00

  • Detection of HLA-G5 secreting cells.

    abstract：:Soluble human leukocyte antigen-G molecules (HLA-G5) can be found in the peripheral blood of healthy females and males, and in other body fluids. To identify cells secreting HLA-G5 we generated a rabbit antiserum against a peptide motif encoded by intron-4 of HLA-G (RaHLA-G/I-4). Utilizing this antiserum as capture an...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2003.08.354

    authors： Rebmann V,Busemann A,Lindemann M,Grosse-Wilde H

    更新日期：2003-11-01 00:00:00

  • Infection with a virus generates a polyclonal immune response with broad alloreactive potential.

    abstract：:Virus-specific T cells have been shown to cross-react with allogeneic HLA (allo-HLA) at a clonal level. However, the impact of a single virus on the allorepertoire has never been investigated at the polyclonal level. We made an inventory of the incidence and specificity of allo-HLA-cross-reactive-virus-specific CD8+ T...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2018.10.014

    authors： van den Heuvel H,van der Meer-Prins EMW,van Miert PPMC,Zhang X,Anholts JDH,Claas FHJ

    更新日期：2019-02-01 00:00:00

  • Increased expressions of CD69 and HLA-DR but not of CD25 or CD71 on endometrial T lymphocytes of nonpregnant women.

    abstract：:To investigate lymphocyte subpopulations and the status of T-cell activation at different phases of the menstrual cycle, lymphocytes in endometrial tissue were analyzed by dual-color flow cytometry in 39 patients. Compared with peripheral blood, the lymphocytes in the endometrium had a higher CD3-/(CD56+ or CD16+) rat...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(94)00105-y

    authors： Chen CK,Huang SC,Chen CL,Yen MR,Hsu HC,Ho HN

    更新日期：1995-03-01 00:00:00

  • Therapeutic potential of FOXP3(+) regulatory T cells and their interactions with dendritic cells.

    abstract：:FOXP3(+) regulatory T cells, a unique subset of T cells, are critical for orchestrating an immune response and preventing self-reactivity. With the increasing prevalence and unsatisfactory treatment of autoimmunity, allergic diseases, cancer and chronic infections, much attention has been focused on understanding thei...

    journal_title：Human immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.humimm.2009.02.007

    authors： Tran DQ,Shevach EM

    更新日期：2009-05-01 00:00:00

  • Large-scale preparation of human anti-third-party veto cytotoxic T lymphocytes depleted of graft-versus-host reactivity: a new source for graft facilitating cells in bone marrow transplantation.

    abstract：:Induction of donor type chimerism in mildly prepared hosts without graft-versus-host disease (GvHD) is a most desirable goal in bone morrow transplantation. We have recently demonstrated in a mouse model that donor veto cytotoxic T lymphocytes (CTLs) can facilitate the induction of donor type chimerism in sublethally ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2005.03.004

    authors： Aviner S,Yao X,Krauthgamer R,Gan Y,Goren-Arbel R,Klein T,Tabilio A,McMannis JD,Champlin R,Martelli MF,Bachar-Lustig E,Reisner Y

    更新日期：2005-06-01 00:00:00

  • Sequential analysis by immunoprecipitation-MALDI-TOF: a novel method for detection and identification of alloantibody specificities.

    abstract：:Alloantibodies are known to influence transplant outcomes. Apart from human leukocyte antigens (HLA), non-HLA targets have been suggested to play a significant role, but little is known about their nature. Here, we present a novel method for identification and characterization of cell surface antigens bound by allorea...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2010.02.006

    authors： Heinold A,Kuehl B,Brenner-Weiss G,Opelz G,Tran TH

    更新日期：2010-05-01 00:00:00

  • DNA typing for class II HLA antigens with allele-specific or group-specific amplification. I. Typing for subsets of HLA-DR4.

    abstract：:DNA sequences that distinguish the subsets of HLA-DR4 are also found on several other alleles. This makes typing of heterozygotes with oligonucleotide probes quite impractical. We have therefore developed a procedure in which, in a first step, DNA of the genes to be analyzed is amplified selectively, using group-speci...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(90)90094-6

    authors： Gao XJ,Fernandez-Vina M,Shumway W,Stastny P

    更新日期：1990-01-01 00:00:00

  • Concomitant augmentation of type 1 CD4+ and CD8+ T-cell responses during successful interferon-alpha and ribavirin treatment for chronic hepatitis C virus infection.

    abstract：:The combined interferon-alpha (IFN-alpha) and ribavirin (IFN-alpha/ribavirin) therapy for chronic hepatitis C virus (HCV) infection results in sustained viral eradication in 31%-64% of the patients. Previous studies have strongly suggested that HCV-specific T-cell responses maybe modulated during this therapy. The obj...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(03)00041-7

    authors： Sreenarasimhaiah J,Jaramillo A,Crippin J,Lisker-Melman M,Chapman WC,Mohanakumar T

    更新日期：2003-05-01 00:00:00

  • MHC haplotype analysis by artificial neural networks.

    abstract：:Conventional matching is based on numbers of alleles shared between donor and recipient. This approach, however, ignores the degree of relationship between alleles and haplotypes, and therefore the actual degree of difference. To address this problem, we have compared family members using a block matching technique wh...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(97)00231-0

    authors： Bellgard MI,Tay GK,Hiew HL,Witt CS,Ketheesan N,Christiansen FT,Dawkins RL

    更新日期：1998-01-01 00:00:00

  • T-cell receptor bias in patients with allergic bronchopulmonary aspergillosis.

    abstract：:CD4(+) Th2 helper cell mediated immune responses have been shown to play a crucial role in the pathogenesis of ABPA. HLA and TCR are the candidate genes, which can influence the specificity of these responses. We have previously established a strong association of HLA DR2/5 in ABPA susceptibility. The study was design...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(02)00361-0

    authors： Chauhan B,Hutcheson PS,Slavin RG,Bellone CJ

    更新日期：2002-04-01 00:00:00

  • Cytotoxic T-cell role in renal allograft rejection and regulation. Renal allograft infiltrating cytotoxic T-cell line with suppressor-inducer function.

    abstract：:We previously investigated the characteristics of renal allograft infiltrating T-cell lines that were propagated from biopsy and nephrectomy specimens designated as IG-Bip and IG-Neph, respectively, or analogous line designated IG-T-eff, which was generated by co-culturing pretransplant recipient blood with irradiated...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(94)00077-4

    authors： Emara MA,Mozes MF

    更新日期：1995-02-01 00:00:00

  • Chronic active autoimmune hepatitis in children. Strong association with a particular HLA-DR6 (DRB1*1301) haplotype.

    abstract：:The association of HLA antigens and type I or "lupoid" CAH-C was investigated in a population of 52 Argentinian Caucasoid patients. When compared with a population of normal individuals of the same ethnic group (n = 197), a significant increase of HLA-DR6 was observed (68.6% in patients vs 17.3% in controls; RR = 12.3...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(94)90008-6

    authors： Fainboim L,Marcos Y,Pando M,Capucchio M,Reyes GB,Galoppo C,Badía I,Remondino G,Ciocca M,Ramonet M

    更新日期：1994-10-01 00:00:00

  • Sustained outgrowth of autotumor-reactive T lymphocytes from human ovarian carcinomas in the presence of tumor necrosis factor alpha and interleukin 2.

    abstract：:Tumor-infiltrating lymphocytes (TIL) freshly isolated from human ovarian carcinomas were phenotyped (up to 75% CD3+ HLA-DR+ cells) and cultured in the presence of recombinant interleukin 2 and tumor necrosis factor alpha. In short-term cultures, an initial outgrowth of CD3+ CD8+ T lymphocytes resulted in the enrichmen...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(90)90022-h

    authors： Vaccarello L,Wang YL,Whiteside TL

    更新日期：1990-06-01 00:00:00

  • HLA-G regulatory haplotypes and implantation outcome in couples who underwent assisted reproduction treatment.

    abstract：:The role of HLA-G in several clinical conditions related to reproduction has been investigated. Important polymorphisms have been found within the 5'URR and 3'UTR regions of the HLA-G promoter. The aim of the present study was to investigate 16 SNPs in the 5'URR and 14-bp insertion/deletion (ins/del) polymorphism loca...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2012.06.002

    authors： Costa CH,Gelmini GF,Wowk PF,Mattar SB,Vargas RG,Roxo VM,Schuffner A,Bicalho Mda G

    更新日期：2012-09-01 00:00:00

  • Association of osteopontin regulatory polymorphisms with systemic sclerosis.

    abstract：:To test the involvement of osteopontin gene (OPN) in systemic sclerosis (SSc) susceptibility, two OPN single nucleotide polymorphisms previously reported to be associated with systemic lupus erythematosus, namely -156G/GG (proximal promoter) and +1239A/C (3' untranslated region (UTR)), were tested in 357 Italian patie...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2011.06.009

    authors： Barizzone N,Marchini M,Cappiello F,Chiocchetti A,Orilieri E,Ferrante D,Corrado L,Mellone S,Scorza R,Dianzani U,D'Alfonso S

    更新日期：2011-10-01 00:00:00

  • TLR4 upregulation underpins airway neutrophilia in smokers with chronic obstructive pulmonary disease and acute respiratory failure.

    abstract：:Activation of Toll-like receptors (TLR) seems to be involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Upon TLR activation the release of defensins, including human beta defensin 2 (HBD-2), may occur. In this study, we explored the innate responses in patients with respiratory failure, with ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2010.09.009

    authors： Pace E,Giarratano A,Ferraro M,Bruno A,Siena L,Mangione S,Johnson M,Gjomarkaj M

    更新日期：2011-01-01 00:00:00

  • Specific "intra-allele" and "intra-broad antigen" human leukocyte antigen alloantibodies in kidney graft transplantation.

    abstract：:Human leukocyte antigen (HLA) antibodies are epitope specific and not antigen specific. This work presents a case of intra-allele (IA) sensitization. A 40-year-old-man underwent transplantion with identical "broad" DR. He was apparently not sensitized to HLA antigens by complement-dependent cytotoxicity (CDC), with on...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2010.05.018

    authors： Muro M,González-Soriano MJ,Salgado G,López R,Boix F,López M,Campillo JA,Martínez P,Botella C,Gimeno L,Minguela A,Alvarez-López MR,Llorente S

    更新日期：2010-09-01 00:00:00

  • Identification of single nucleotide polymorphisms in the tumor necrosis factor (TNF) and TNF receptor superfamily in the Korean population.

    abstract：:The tumor necrosis factor (TNF) and TNF receptor (TNF-TNFR) superfamily plays crucial roles in immune regulation and host immune responses. The superfamily has been also associated with many immune-mediated diseases such as asthma, rheumatoid arthritis, inflammatory bowel disease, and diabetes. In order to investigate...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2004.04.005

    authors： Cho SM,Kim J,Ryu HJ,Kim JJ,Kim HH,Park JH,Kim HT,Kim KH,Cho HY,Oh B,Park C,Kimm K,Jo I,Lee JE,Shin HD,Lee JK

    更新日期：2004-07-01 00:00:00

  • Detection of HLA restricted anti-minor histocompatibility antigen(s) reactive cells from skin GVHD lesions.

    abstract：:Recipients of marrow grafts from HLA-identical, (including MLC non-reactive) sibling donors have a 40-50% incidence of acute graft vs. host disease (GVHD); the involvement of non-HLA linked, minor histocompatibility antigens (miHA) has been implicated. One of the target tissues of GVHD is the skin where morphological ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(84)90064-8

    authors： Reinsmoen NL,Kersey JH,Bach FH

    更新日期：1984-12-01 00:00:00

  • Isolation and allelic polymorphism of cDNA clones and genomic clones of HLA-DP heavy and light chains.

    abstract：:From a human cDNA library constructed from consanguineous HLA-homozygous cell line AKIBA (HLA-A24, Bw52, DR2, Dw12, Cp63 Cp63: a new DP type), cDNA clones encoding the heavy and light chains of an HLA-DP Cp63 alloantigen were isolated and analyzed by restriction enzyme mapping and nucleotide sequence determination. Al...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(86)90286-7

    authors： Ando A,Inoko H,Kimura M,Ogata S,Tsuji K

    更新日期：1986-11-01 00:00:00

  • TCRBV12 genes are polymorphic but the protein products encoded by each gene are conserved.

    abstract：:The human TCRBV gene complex contains three BV12 genes and all are polymorphic. There are two alleles of BV12S4; one allele contains a stop codon in the coding region which precludes its expression. There are four alleles of BV12S2 and two alleles of BV12S3 genes; however, the substitutions present in both genes are c...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(94)00152-g

    authors： Deulofeut H,Wilderson JL,Barron KS,Robinson MA

    更新日期：1995-07-01 00:00:00

  • Human leukocyte antigen class I alleles and haplotypes associated with primary hepatocellular carcinoma in persistent HBV-infected patients.

    abstract：:Many studies have shown that Human leukocyte antigen (HLA) class I alleles are associated with the development of various cancers. However, its role in hepatocellular carcinoma (HCC) is still unknown. To investigate whether HLA class I allelic polymorphism is related to the development of hepatitis B virus(HBV)-associ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2013.02.007

    authors： Pan N,Chen K,Qiu J,Sun H,Xu J,Miao F,Shi Q,Jiang W,Jin H,He Y,Xie W,Zhang J

    更新日期：2013-06-01 00:00:00

  • TAP1 and TAP2 gene polymorphisms and HLA-TAP haplotypes in Koreans based on 90 families.

    abstract：:We have investigated the polymorphism of TAP genes and the distribution of human leukocyte antigen (HLA)-TAP haplotypes in 90 Korean families (180 parents and 233 children), previously typed for HLA class II alleles. TAP1 (codons 333 and 637) and TAP2 (codons 379, 565, 577, 651, 665, and 687) typings were carried out ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2005.07.001

    authors： Whang DH,Park H,Roh EY,Park MH

    更新日期：2005-09-01 00:00:00

  • Improving the performance of virtual crossmatch results by correlating with nationally-performed physical crossmatches: Obtaining additional value from proficiency testing activities.

    abstract：PURPOSE:When donor specific HLA antibodies (DSA) are identified, the predictive value of whether a certain strength of reactivity (mean fluorescence intensity, MFI) leads to a positive crossmatch is uncertain. To determine this, we compared the DSA MFI results we generated locally for nationally distributed proficiency...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/j.humimm.2018.05.009

    authors： Wrenn SM,Marroquin CE,Hain DS,Harm SK,Pineda JA,Hammond PB,Shah DH,Hillyard SE,Fung MK

    更新日期：2018-08-01 00:00:00

  • Bone marrow transplantation in Brazil.

    abstract：:The first program of bone marrow transplantation in Latin America was started 5 years ago at the Federal University Hospital, Curitiba, Paraná. The results of 62 patients who underwent bone marrow transplantation are presented and discussed. ...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/0198-8859(85)90240-x

    authors： Ferreira E,Dulley FL,Morsoletto F,Neto JZ,Pasquini R

    更新日期：1985-11-01 00:00:00

  • A clonal culture assay for human cord blood lymphohematopoietic progenitors.

    abstract：:We describe a two-step clonal culture assay system for human lymphohematopoietic progenitors present in umbilical cord blood which are capable of differentiation along both myeloid and B-lymphoid lineages. Human cord blood CD34+ cells were plated in methylcellulose in the presence of stem cell factor (SCF), granulocyt...

    journal_title：Human immunology

    pub_type： 杂志文章

    doi：10.1016/s0198-8859(98)00094-9

    authors： Yoshikawa Y,Ikebuchi K,Ohkawara J,Hirayama F,Yamaguchi M,Sato N,Mori KJ,Kasai M,Sekiguchi S

    更新日期：1999-01-01 00:00:00