Abstract:
:DNA sequences that distinguish the subsets of HLA-DR4 are also found on several other alleles. This makes typing of heterozygotes with oligonucleotide probes quite impractical. We have therefore developed a procedure in which, in a first step, DNA of the genes to be analyzed is amplified selectively, using group-specific primers. In the case of DR4-DRB1, a primer matching codons 5 to 13 when used in the polymerase chain reaction resulted in products that were entirely suitable for typing for the DR4 subsets. Using appropriate probes, eight distinct subsets were identified. However, only six of them were represented in a normal North American Caucasian panel. In conjunction with a method for rapid DNA extraction, the procedure offers a simple, highly specific and reproducible method for determining subtypes of HLA-DR4 that at present cannot be recognized by serologic methods.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Gao XJ,Fernandez-Vina M,Shumway W,Stastny Pdoi
10.1016/0198-8859(90)90094-6subject
Has Abstractpub_date
1990-01-01 00:00:00pages
40-50issue
1eissn
0198-8859issn
1879-1166pii
0198-8859(90)90094-6journal_volume
27pub_type
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